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Citations for Thermo Fisher :
5951 - 6000 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then incubated with a 1:10,000 dilution of goat anti-human Fc HRP secondary antibody (Invitrogen, A18817) in PBST for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: ... PBMCs were labeled with 5 μM CFSE and activated with Dynabeads™ Human T-Activator CD3/CD28 (Thermo Fisher Scientific). To evaluate immunosuppressive potential ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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Cell cycle plasticity underlies fractional resistance to palbociclib in ER+/HER2- breast tumor cellsbioRxiv - Cancer Biology 2023Quote: ... T47D (ATCC HTB-133) or primary cells from human tumors were fixed by adding 8% PFA (Thermo Scientific cat#28908) directly to the samples (1:1 v/v with media ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ cells were combined at a 1:1 ratio and activated using anti-human CD3/CD28 Dynabeads (Invitrogen, #40203D) at a 3:1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... Detroit 562 human nasopharyngeal epithelial cells (ATCC ref# CCL-138) were maintained in DMEM supplemented with 1x sodium pyruvate (Gibco) and 1x GlutaMax (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... commercially available kits were purchased and the assays done following the manufacturer’s protocol: IL-1β (Mouse: BMS6002; Human: BMS224INST, Thermo Fisher) and HMGB1 (Mouse ...
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bioRxiv - Genetics 2023Quote: ... was extracted from skin fibroblasts derived from two different Welsh Mountain ponies that were overexpressing human SOX11 (as described above) using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Cancer Biology 2023Quote: ... of the target genes together with a human GAPDH endogenous control and the use of 7500 SDS software (Applied Biosystems). The primers used in this study were as follows ...
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bioRxiv - Bioengineering 2023Quote: Human mammary gland epithelial cell adenocarcinoma cell line MDA-MB-231 was maintained in F-12K (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The pENTR-hMCAK construct was generated by amplifying the coding sequence of human MCAK by PCR and inserting it into a pENTR entry clone using the pENTRTM/D-TOPO kit (ThermoFisher). The pFB-6HF2hMCAK construct was created from pFB-6HFRET2-DEST vector and pENTR-hMCAK constructs using Gateway® cloning technology (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... Clathrin Heavy Chain siRNA (Human CLTC, sequence: GGUUGCUCUUGUUACG, ID: s475) and Negative Control#1 siRNA Silencer Select were purchased from ThermoFisher Scientific.
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bioRxiv - Bioengineering 2023Quote: Human mammary gland epithelial cell adenocarcinoma cell line MDA-MB-231 was maintained in F-12K (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: ... TJC4) was detected using a 1:500 dilution of goat anti-human Alexa Fluor 488 or Alexa Fluor 647 (Invitrogen). Secondary antibodies were incubated for 15 min at 4°C in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were labelled with phycoerythrin (PE)-conjugated anti-human-HLA-ABC (clone W6/32, Invitrogen 12-9983-42; 1:20), biotin-conjugated anti-mouse-H-2Kb (clone AF6-88.5 ...
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bioRxiv - Microbiology 2023Quote: ... Human alveolar A549 cells were seeded in an 8 well chamber slide (Lab-Tek II Chamber Slide w/ Cover, NUNC), and once confluent ...
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bioRxiv - Cell Biology 2023Quote: ... MiniBAR-GFP sta-ble cell line was transfected with 25 nM of siRNAs targeting luciferase (5’-CGUACGCGGAAUACUUCGA-3’) or human Rab35 (5’-GCUCACGAAGAACAGUAAA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... human pluripotent stem cells were dissociated using Accutase and plated at high density on Geltrex (#A14133-02, Thermo Fisher Scientific) coated wells ...
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bioRxiv - Cancer Biology 2023Quote: ... Viably frozen primary AML cells were thawed and CD3-depleted with biotinylated anti-human CD3 (SK7) and biotin-binder Dynabeads (Invitrogen) and subsequently injected via the lateral tail vein into 2.8 Gy irradiated (24h before transplant ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human endogenous PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T) cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Immunology 2023Quote: ... The low and high molecular weight membranes proteins were incubated with respective primary antibodies (Rabbit anti-human BAIAP2L1, PA5-54000, Invitrogen) or (Goat anti-mouse GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells or MiniBAR-GFP stable cell lines were transfected with 25 nM of siRNAs targeting either luciferase or human Mini-BAR (5’-CUGCAAAUUUUACGGAUCA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed 5x and incubated with 100 µl/well with an HRP-conjugated anti-human IgG Fc antibody (1:10000 dilution, Invitrogen) for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... purified Treg cells (5 × 104) were cultured with Dynabeads Human T-activator CD3/CD28 (12.5 μl/ml) (Thermo Fisher Scientific) and IL-2 (100 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Human TSC media (TSCm) used herein was previously described in 70 with slight modifications: 470 ml DMEM/F12 (Invitrogen 21331), 5 ml Commercial N2 supplement (Invitrogen 17502048) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Genomics 2023Quote: The human lung fibroblast cell line IMR-90 was purchased from ATCC and cultured in EMEM (ATCC) with 10% FBS (Gibco) and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... Unbound ACE2-Fc was removed by washing the cells with ice cold media and the bound ACE2 detected using goat anti-human secondary antibody conjugated to Alexa647 (ThermoFisher Scientific Cat ...
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bioRxiv - Genomics 2023Quote: ... These mRNAs were generated from cloned human cDNAs inserted into pCS2+ and in vitro transcribed using mMessage mMachine SP6 kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Transcriptome libraries were generated using automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The transcriptome libraries were barcoded ...
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bioRxiv - Cell Biology 2023Quote: NK cells were transfected with scrambled or ON-TARGET plus human WWTR1 SMARTpool siRNA from Dharmacon Inc, (CCGCAGGGCUCAUGAGUAU, GGACAAACACCCAUGAACA, AGGAACAAACGUUGACUUA, CCAAAUCUCGUGAUGAAUC) using the Neon Transfection System (Invitrogen) and according to the manufacturer recommended parameters ...
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bioRxiv - Genomics 2023Quote: Pulmonary arterial adventitial cells isolated from idiopathic PAH and control lungs were obtained from PHBI and grown in Human Vascular Smooth Muscle Cell Basal Medium (M231500, ThermoFisher) supplemented with Smooth Muscle Growth Supplement (S00725 ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for 2 hr at room temperature with the following antibodies at the manufacturer-recommended dilutions in 1× TFP Perm/Wash buffer: APC- eFluor780 anti-human CD3 (clone UCHT1, Invitrogen); PerCP-Cy5.5 anti-human CD4 (clone SK3 ...
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bioRxiv - Developmental Biology 2023Quote: ... floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher, Cat#12634010), 2 mM Glutamax (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The brain sections were then incubated with goat anti-mouse Alexa Fluor 488 and goat anti-human Alexa Fluor 647 (1:500, Invitrogen). Following secondary staining ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The donor template for homologous recombination was generated by PCR amplification of a human DNA sample carrying 51 CAG repeat into the pCR-Blunt II TOPO plasmid (Invitrogen). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... in 6-well tissue culture plates at 37°C with 5% CO2 and 5% O2 They were grown in human embryonic stem cell (hESC-10) medium made up of a base of DMEM/F12 (50:50 Gibco) with 20% knock-out serum replacement (KOSR ...