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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... The integrity and quantity of RNA was verified through gel electrophoresis and NanoDrop One/OneC spectrophotometer (Thermo Fisher Scientific, US). Total RNA was submitted to Novogene Hong Kong for library construction and sequencing on the Illumina Novaseq6000 (PE150 ...
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bioRxiv - Microbiology 2024Quote: ... with the MBTuni-12 [5’-ACGCGTGATCAGCAAAAGCAGG] and MBTuni-13 [5’-ACGCGTGATCAGTAGAAACAAGG] primer sets for Influenza A (59, 60) with the SuperScript III One-Step RT-PCR kit as per manufacturers guidelines (Invitrogen).
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... The total DNA quantity and quality were evaluated using a NanoDrop One UV-Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA) and a Qubit 3.0 Fluorometer (Invitrogen Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... One microgram of total RNA was reverse-transcribed to cDNA using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher) and stored at −20°C ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... grown in semi-suspension in 150 mm plates in a mixture of serum free media (ACF CHO medium EX-CELL Merck, CHO ONE media Capricorn Scientific, OptiMEM-CHO Gibco), supplemented with 1% Primatone (Merck) ...
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bioRxiv - Evolutionary Biology 2024Quote: Fragments of Po-exd and Po-N were amplified using standard PCR protocols and cloned using a TOPO TA Cloning Kit using One Shot Top10 chemically competent Escherichia coli (ThermoFisher) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... and one 7 mm surgical cotton ball was placed in each well of the 96 well plates (Thermo Fisher scientific). Black plates were used to minimize light reflection and penetration ...
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Assessment of the transcriptional regulation of EMT and MET through accessible transposable elementsbioRxiv - Genomics 2024Quote: ... One microgram of total RNA was used for cDNA synthesis using the Maxima First Strand cDNA Synthesis kit (Thermo Scientific). Primers and probes used for qPCR were previously reported (Alotaibi et al ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of RNA was used for cDNA synthesis using a ML-MLV reverse transcriptase (Invitrogen, San Diego, CA, USA) with a mixture of random hexamer and oligo-(dT)18 primers ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription and qRT-PCR was performed in one step using the SuperScript III Platinum Kit (Thermo Fisher Scientific, #11732088) on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Biophysics 2021Quote: ... about ~5 μg of bacmid were transfected using 6 μl of CellfectinII reagent (Thermo Fisher Scientific). 5 days after initial transfection ...
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bioRxiv - Cancer Biology 2021Quote: Cells seeded in 6-well dishes were washed twice with HBSS (Cat# 14025-134, Life Technologies) and collected using a manual cell lifter ...
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bioRxiv - Cancer Biology 2021Quote: ... HRas samples were heated at 95 °C for 6 min using a MiniAmp thermocycler (Applied Biosystems), resulting in protein denaturation ...
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bioRxiv - Cancer Biology 2021Quote: ... A375 cells were seeded in the wells of non-treated 6-well-plate (ThermoFisher, Waltham, MA) coated with POSTN (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR for both mRNAs and miRNAs was performed on a QuantStudio 6 Flex machine (Applied Biosystems) using SYBR-green fluorescent dye (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 X 105 PC3 or MCF7 cells were plated in either 6 well culture dishes (Nunc™ ...
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bioRxiv - Developmental Biology 2021Quote: ... and day 6 control and Pogz-/- ESC-derived EBs were collected and treated with Trizol (Invitrogen). RNAs were quantified by a Nanodrop instrument ...
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bioRxiv - Developmental Biology 2020Quote: ... was supplemented to the medium for the first 6 days and B-27 Supplement (Gibco, 17504044) was used afterwards ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted from pools of glands form 6 mice using TRIzol Reagent (Invitrogen, Carlsbad, CA). RNA from HEK293 cells was also extracted with TRIzol Reagent (12) ...
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bioRxiv - Molecular Biology 2020Quote: ... 6’-diamidino-2-phenylindole (DAPI) for nuclear staining (Fluoromount-G™ Mounting Medium, with DAPI, Invitrogen), and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were performed using either a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, USA) with PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Co-transfection was carried out in 6-well dishes using lipofectamine 2000 reagent (Thermo Fisher Scientific) by adding 1.2 μg of HLJ1-shRNA and 2 μg of IL-12p35 plasmid DNA according to the protocol of the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of 2-6 μg/mL S was plated onto 384-well Nunc Maxisorp (ThermoFisher) plates in PBS and sealed overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... qRT-PCR was carried out using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) and SYBR Green GoTaq qPCR Master Mix (Promega) ...
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bioRxiv - Biophysics 2019Quote: ... 30 μg protein was separated on Novex Wedgewell 6% Tris-Glycine gels (Life Technologies, cat #XP00060box) and subsequently transferred to PVDF Transfer Membrane ...
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bioRxiv - Genetics 2019Quote: ... for 20 min at room temperature before separating on a 6% retardation gel (Thermo Fisher Scientific). With regard to competitive EMSA ...
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bioRxiv - Biochemistry 2021Quote: ... ∼105 cells were seeded in a 6-well plate in a reduced-serum media (Optimem, Gibco), grown for 24h and treated with 100µM H2O2 dissolved in Optimem (37°C ...
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bioRxiv - Biophysics 2021Quote: ... 2.5 × 105 MCF7 cells were seeded per well in a 6-well Lab-Tek chamber (Nunc) until 80% confluence was reached ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5 mM NEAA + DMEM/Sodium Bicarbonate (2.438 g/L) media in 6-well plates (Thermo Fisher). After 48 hours cells were scratched ...
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bioRxiv - Neuroscience 2020Quote: ... 150,000 neurons/well were added to 6-well plates in NBA plus [Neurobasal-A medium (Gibco) supplemented with Glutamax (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 cm or 10 cm cell culture dishes or 96 well flat-bottom plates (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer’s protocol on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems), with data analyzed using GraphPad Prism 6 software ...
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bioRxiv - Bioengineering 2021Quote: ... cells were cultured in Nunc™ cell-culture treated 6-well plates (140675; Thermo Scientific™). HepatoSTIM™ medium was supplemented with 10 ng mL−1 of recombinant human TGF-β1 (100-21 ...
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bioRxiv - Microbiology 2020Quote: ... and gene expression was quantified using relative standard curves on a QuantStudio 6 Flex (Applied Biosystems) with PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... A qRT-PCR assay was performed using the QuantStudio™ 6 Flex system (Thermo Fisher Scientific) and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR was then conducted using QuantStudio 6 Flex Real Time PCR Instrument (Applied Biosystems, USA). The cDNA was diluted 1:10 and 5 µl of the solution were used as template for qRT-PCR with SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX cells were plated in 6-well ULA plates (Fisher Scientific, cat. no. 07-200-601) at 2.5 × 105 cells/well and topped off with 1 mL of either EpiCult™ Medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... spleens from C57Bl/6 mice were smashed through a 70 µm cell strainer (Thermo Fisher Scientific). After red blood cell lysis (1:10 dilution for 3 min at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instruction and carried out on Quant Studio 6 Flex System (Applied Biosystems). Relative microRNA expression was calculated by the 2−ΔΔCT method and RnU6b was used as the internal control for normalization.
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bioRxiv - Cell Biology 2022Quote: ... YPD plates containing 0.33 M acetate pH 6 were prepared by adding 3M sodium acetate (Ambion) to 1L sterilized YPD ...
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bioRxiv - Developmental Biology 2022Quote: ... Each well was incubated with pre-warmed enzymatic mix of 6 mg/ml Dispase (Gibco, 17105041) and 1 mg/ml Liberase TH Research Grade (Roche ...
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bioRxiv - Developmental Biology 2022Quote: A total of 500K cells were seeded per well of 6-well plate (Thermo Scientific, # 1483210) to reach confluent monolayer ...
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bioRxiv - Bioengineering 2022Quote: ... levels of mIL-6 and mTNF-α were estimated using their respective ELISA kits (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was run on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... either alone or with anti-IL-1β or anti-IL-6 neutralizing antibodies (Thermofisher, Waltham, MA), and ensuing protein lysates blotted for phosphorylated STAT-3 (pSTAT3) ...
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bioRxiv - Cell Biology 2022Quote: ... 3-6 dpf larval zebrafish were anesthetized using 0.2 mg/mL Tricaine-S (Fisher Scientific, NC0872873) solution ...
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bioRxiv - Cancer Biology 2020Quote: ... and Type 6 primary probes targeting MYCN were designed and synthesized by Affymetrix (Supplementary Table 2). Hybridization was performed according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... on 6-well plates (Thermo Fischer: 140675) coated with Geltrex basement membrane matrix (Thermo Fisher, A1413302). Cells were passaged upon reaching 60-70% confluency ...
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bioRxiv - Developmental Biology 2020Quote: ... Other media components included Insulin-Transferrin-Selenium-Ethanolamine (ITS-X; Thermo Fisher Scientific; days 6-10), retinoic acid (RA ...
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bioRxiv - Molecular Biology 2020Quote: ... Cloned vectors (1.5 μg each per 6 well plate well) were transfected using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions into U2OS PER2::LUC cells ...