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Citations for Thermo Fisher :
5901 - 5950 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The viral DNA was transfected into UT7/Epo cells (2×105) seeded one day prior using lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed and part of the mutated region within the ZIKV genome was PCR amplified using SuperScript III One-Step RT-PCR System with Platinum Taq Polymerase (Invitrogen) together with either the 5’-aagtggagaaaaagatggg-3’ (WT and SCR ...
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bioRxiv - Microbiology 2021Quote: ... We performed one-step multiplex real-time PCR using TaqPath™ 1-Step Multiplex Master Mix (#A28526, Thermo Fisher Scientific), targeting three different SARS-CoV-2 genes with primer and probe sets specific for spike (S) ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... Each gene segment was synthesized by gene specific primers (primers available upon request) using SuperScript III One-Step RT-PCR System (Invitrogen).
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bioRxiv - Plant Biology 2020Quote: Regions screened for transcription factor binding via Yeast One-Hybrid were first inserted into pENTR 5’TOPO TA entry vector (Thermofisher) and subsequently placed into the pMW2 and pMW3 destination vectors containing HIS3 and LACZ marker genes respectively (Deplancke et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Microarray probes were synthetized in one cycle of RNA amplification in which molecules were labeled (Affymetrix microarray station, Affymetrix, CA). The labeled microarray probes were hybridized on a Mouse Clariom S (mm10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Microarray probes were synthetized in one cycle of RNA amplification in which molecules were labeled (Affymetrix microarray station, Affymetrix, CA). The labeled microarray probes were hybridized on a Mouse Clariom S (mm10 ...
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bioRxiv - Molecular Biology 2019Quote: The SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (catalog- no. 12574-026, Invitrogen™ ...
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bioRxiv - Evolutionary Biology 2019Quote: ... small RNA fraction and the large one were isolated using the microRNA & small RNA Isolation kit (Thermo Fisher Scientific, Germany). Traces of genomic DNA were removed from 10 µg of RNA by digestion in a total volume of 500 µl containing 20 units of TURBO DNase ...
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bioRxiv - Cancer Biology 2019Quote: ... Fast SYBR™ Green quantitative PCR was performed on a Step One™ Plus Real-Time PCR System (Applied Biosystems). Sequence of all RT-qPCR primers are shown in Supplementary Table 1.
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bioRxiv - Cell Biology 2019Quote: ... cells were harvested by using trypsin one day after transfection and washed once in HBSS buffer (containing calcium and magnesium, Gibco) supplemented with 1% (w:v ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... One testis was randomly selected for whole tubule immunofluorescence and was immediately placed in cold PBS (Gibco, Cat# 10010-023) on ice ...
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bioRxiv - Genomics 2021Quote: ... Quality and concentration of the extracted DNA were evaluated using NanoDrop™ One/OneC UV-Vis Spectrophotometer (Thermo Scientific™). Library construction and whole genome sequencing were conducted by Beijing Genomics Institute (BGI ...
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bioRxiv - Cell Biology 2021Quote: ... one-step quantitative real-time PCR was performed using the SuperScript III Platinum SYBR Green One-Step qRT–PCR Kit (Invitrogen) with primers specific for TRS (listed above ...
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bioRxiv - Microbiology 2021Quote: ... qPCRs measurements were conducted in an Applied Biosystems Step One Real-Time PCR system (Life Technologies, Grand Island, NY, USA). The thermal cycling protocol was performed as follows ...
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bioRxiv - Microbiology 2021Quote: ... Reporter cells were then grown at 37°C for one hour in a Multiskan GO 96-well plate reader and shaker incubator (ThermoFisher), monitoring growth of reporter cells over time at OD650 ...
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bioRxiv - Immunology 2021Quote: RT-PCR reactions were performed using 4 µl of resuspended RNA in a 20 µl reaction volume using the Superscript III one step RT-qPCR system with Platinum Taq Polymerase (Invitrogen) with 400 nM concentrations of each primer and 200 nM of probe ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... One μg of RNA was reverse transcribed using the high-capacity cDNA reverse transcription kit (Applied Biosystems; Grand Island, NY) following manufacturer’s protocols ...
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bioRxiv - Pathology 2021Quote: ... Real-time PCR assays were run on an ABI Prism One step real-time PCR machine (Applied Biosystems, Courtaboeuf, France). Normalization was performed using 36B4 as a reference gene ...
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β-importins Tnpo-SR and Cadmus and the small GTPase Ran promote ovarian cyst formation in DrosophilabioRxiv - Developmental Biology 2021Quote: ... dissected ovaries were incubated for one hour at room temperature in Grace's media containing 10 μM 5-ethynyl-2′-deoxyuridine (EdU; Life Technologies). The following primary antibodies were diluted in blocking solution and used overnight at 4°C ...
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bioRxiv - Genomics 2019Quote: Toxoplasma-exposed and unexposed monocytes from one donor were harvested and washed three times in cold PBS supplemented with 0.1% bovine serum albumin (BSA, Thermo Fisher). The scRNA-seq libraries were generated using the Chromium Single Cell 3’ Library & Gel Bead Kit v.2 (10x Genomics ...
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bioRxiv - Microbiology 2021Quote: ... Standards for nifH reactions were obtained by amplifying one cloned nifH sequence with flanking regions of the plasmid vector (TOPO TA cloning Kit, Invitrogen). Standard curves were obtained by serial dilutions (E = 0.9 – 1.1 ...
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bioRxiv - Microbiology 2021Quote: ... The enrichment phase was performed by using the One-Touch 2 device with the Ion Sequencing 400 Kit according to the manufacturer’s instruction (Life Technologies) (Moitinho et al ...
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bioRxiv - Immunology 2020Quote: ... Wells were washed three times with 1xDPBS followed by a one hour incubation with horse radish peroxidase-conjugated goat anti-human total Ig at a dilution of 1:6000 (Invitrogen). Wells were then washed four times with 1xDPBS followed by a 5-15 minute incubation with TMB substrate (SeraCare) ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA purity was confirmed via an A260/A280 measurement (target ≥1.8) using NanoDrop™ One (Thermo Scientific™, DE), and DNA concentration was quantified using Qubit® 3.0 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2021Quote: RNA was isolated from 12 fetal brains (one per litter) using TRIzol Reagent as per the TRI Reagent Protocol (Invitrogen), and delivered to the Genomics Shared Resource lab at The Ohio State University Comprehensive Cancer Center ...
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bioRxiv - Immunology 2021Quote: ... cells were alternatively pre-activated with IL-2 as above and one of: 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads Human T-Expander or Human Treg Expander) ...
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bioRxiv - Molecular Biology 2020Quote: ... pU6-2-gRNA plasmids (Drosophila Genomics Resource Center stock# 1363) were amplified in One Shot Top 10 competent E.coli cells (Invitrogen# C404010) on SOC Medium (Corning# 46003CR) ...
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bioRxiv - Microbiology 2021Quote: ... double-stranded cDNA was synthesized by real-time reverse-transcription (RT-PCR) using Superscript III platinium One-step Quantitative RT-PCR System (Invitrogen) as described previously with minor modifications (Corman et al. ...
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bioRxiv - Molecular Biology 2020Quote: One μg of DNase-treated total RNA was reverse transcribed using the M-MLV Reverse Transcriptase kit (Thermo Fisher Scientific) according to manufacturer’s protocol with the use of random hexamers to prime the reaction ...
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bioRxiv - Microbiology 2021Quote: ... one tube was prepared with 16 μg of plasmid diluted in 1.5 mL of OPTI-Mem (Gibco; Ref# 31985-070). The second tube was prepared with PEI (1mg/mL ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Microbiology 2021Quote: One of the F-buffer cultures was then treated with crosslinker DSP (dithiobis(succinimidyl propionate)) (Thermo Scientific Catalog number 22585) to a final concentration of 1 mM and incubated for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Immunology 2020Quote: ... qPCR reactions were run on a Step one Plus real-time PCR apparatus using the absolute quantification program (Applied Biosystems), with the following conditions for FAM-TAMRA probes ...
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bioRxiv - Genomics 2021Quote: ... Clinical isolates were subcultured for 14 days before inoculation onto one of two CRyPTIC designed 96-well microtiter plates manufactured by ThermoFisher. The first plate used (termed UKMYC5 ...
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bioRxiv - Microbiology 2021Quote: RT-PCR was performed using SuperScript™ III One-Step qRT-PCR System kit (commercial kit #1732-020, Life Technologies) with primers ORF1ab_Fw ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of total RNA was used for poly-A selection with a Dynabeads mRNA purification kit (Invitrogen, CA, USA) followed by cDNA generation (NEB 1st strand and NEB 2nd strand ultra directional Kits ...
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bioRxiv - Microbiology 2022Quote: ... This DNA-free RNA was then subjected to RT-PCR using the SuperScript III One-Step RT-PCR system with Platinum Taq DNA Polymerase kit (Invitrogen) with primers specific to the gene of interest (Supplementary Table 2).
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bioRxiv - Microbiology 2022Quote: ... The culture was split in half with one set being used for genomic DNA extraction while the other was used for total RNA extraction using TRIzol (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the nano-LC system was operated in one-column setup with an EasySpray PEPMAP RSLC C18 column (Thermo Fisher Scientific) kept at the constant temperature of 45°C ...
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bioRxiv - Neuroscience 2022Quote: ... and one CTL (SP11) were differentiated into dopaminergic neurons using the three-step system PSC Dopaminergic Neuron Differentiation Kit (GIBCO) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: The DNA origami was folded in a one-pot reaction by gradually decreasing the temperature using a Proflex 3×32-well PCR system (ThermoFisher). The scaffold strands (p7249 and p7560 variants of single-stranded M13mp18 ...
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bioRxiv - Cancer Biology 2022Quote: ... UM-SCC-47 cell line was cultured in three parts of Hams F-12 nutrient mixture medium and one part of DMEM with 10% FCS (Gibco) and 100 U/mL penicillin–streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... The terminated LR clonase reactions were used to transform One Shot™ MAX Efficiency™ DH5α-T1R competent cells (Invitrogen) and transformants were plated on ampicillin agar plates ...
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bioRxiv - Cell Biology 2022Quote: ... The terminated BP clonase reactions were used to transform One Shot™ MAX Efficiency™ DH5α-T1R competent cells (Invitrogen) and transformants were plated on kanamycin agar plates ...
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bioRxiv - Cell Biology 2022Quote: ... AS ONE) were transfected with 1.5 μg expression plasmids using Lipofectamine LTX with Plus Reagent (15338-100, Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Quality and quantity of the isolated RNA were measured using the NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific). 1μg of RNA was reverse transcribed into cDNA using the qScript cDNA Synthesis Kit (Quantabio) ...