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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Coding sequences were amplified using total RNA from 7 days post-fertilization (dpf) zebrafish using the SuperScript IV One-step RT-PCR kit (ThermoFisher) using the primers listed in Table S1 ...
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bioRxiv - Bioengineering 2023Quote: ... The concentration of RNA in each sample was measured using a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific). Reverse transcription of RNA was performed using iScript™ Select cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... according to Agilent’s One-Color Gene Expression Microarray Protocol followed by the quantification using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific). The hybridization of 600 ng labeled cRNA to the microarray slide was carried out at 65°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in one or more primary antibody: rabbit anti-GFP (1:2000; Molecular Probes Cat# A-11122, RRID:AB_221569), chicken anti-GFP (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... two mouse monoclonal antibodies were utilized: one for the purpose of IP/ immuno-blotting (Thermo Fisher Scientific catalog # MA5-25915) (Figure 3A and S3A) ...
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bioRxiv - Microbiology 2023Quote: ... We preincubated (at 37°C for one h) 40 µl of half-log10 serially diluted Abs in Minimum Essential Media (MEM) media (Gibco) supplemented with 10mM Hepes (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... We preincubated (at 37°C for one h) 40 µl of half-log10 serially diluted Abs in Minimum Essential Media (MEM) media (Gibco) supplemented with 10mM Hepes (Gibco) ...
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bioRxiv - Physiology 2023Quote: qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems) on an Applied Biosystems 7900HT real-time PCR machine in 96-well-plate format ...
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bioRxiv - Developmental Biology 2023Quote: ... The total concentration of isolated RNA was measured by using a NanoDrop One device (Fisher Scientific, catalog #13-400-519). The RNA was then subjected gDNA removal using DNase I enzyme mix following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: One mg of mCherry mRNA was taken for cDNA synthesis using Superscript III first strand synthesis kit (Invitrogen catalog # 18080051). The cDNA synthesis was done as per the manufacturer’s recommended protocol ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the samples protein content was measured at 280 nm using NanoDrop One (NanoDrop Ins., Thermo Fisher Scientific, Waltham, MA, USA). The cortisol data are expressed in pg/mg protein.
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bioRxiv - Bioengineering 2023Quote: ... the tissue was cut down the center and one half was placed in RNAlater (AM7020; ThermoFisher Scientific, Waltham, MA, USA) for future RNA isolation ...
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bioRxiv - Genomics 2023Quote: ... The quality and purity of the extracted DNA were assessed using NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Scientific). Purified genomic DNA was sent to Beijing Genomics Institute (16th Dai Fu Street ...
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bioRxiv - Genetics 2023Quote: ... One microgram of total RNA was reverse transcribed using the Taqman Reverse Transcription kit (Applied Biosystems; Cat# N8080234; Waltham, MA) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... (donkey and rabbit -HRP [1:500] or Goat anti mouse-HRP [1:500] and incubated for one hour at room temperature Membranes were developed with enhanced chemiluminescence substrate (ECL) and visualized on an iBright FL1000 (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... The quantity and quality of eluted DNA were measured using NanoDrop One spectrophotometer and Qubit 2 (both by ThermoFisher Scientific) to ensure that samples meet the minimum requirements for sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... DNA removal from RNA fractions was confirmed by RT-PCR using the SuperScript IV One-Step RT-PCR System (Invitrogen) with and without addition of SuperScript IV RT Mix ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative Real-Time PCR was performed using KAPA SYBR FAST One-Step kit (KAPA) on a QuantStudio 7 Flex (ThermoFisher) with the following cycling conditions ...
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bioRxiv - Neuroscience 2023Quote: ... One microgram of total RNA was reverse transcribed to cDNA using poly (T) primer and SuperScript III Reverse Transcriptase (Invitrogen). Quantitative PCR was performed on a LightCyler 480 (Roch Applied Science) ...
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bioRxiv - Cell Biology 2023Quote: ... then were excised and placed in a C-Tube (Miltenyi) (one C-tube/mouse) containing 5mL lung digest solution (5mL phenol free DMEM (Gibco), Dispase (Roche 04942078001) ...
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bioRxiv - Cancer Biology 2023Quote: ... and reverse transcription of total RNA was performed with SuperScript™ IV One-Step RT-PCR System (Thermo Fisher Scientific). Quantitative PCR was performed using the SYBR Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used in reverse transcription reactions using the Superscript III RT (Invitrogen, Carlsbad, CA, USA) to obtain the first strain cDNA ...
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bioRxiv - Physiology 2024Quote: ... One-half μg of RNA was converted to cDNA using the Verso cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA). PCR amplification of the cDNA was performed by quantitative real-time PCR using TrueAmp SYBR Green qPCR SuperMix (Smart Bioscience ...
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bioRxiv - Physiology 2024Quote: ... The following day slides were washed with 1XPBS and incubated for one hour at room temperature with Alexa-Fluor555 anti-rabbit secondary antibody (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were then stained for one and a half hours with Alexa Fluor 568 donkey anti-goat (1:400; A11057, Invitrogen) and DAPI (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated for one hour with 5% normal donkey serum (NDS) in TKBPS (KBPS with 0.25% Triton X-100 (Fisher Scientific)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Specificity and integrity of dsRNA were checked on 1% agarose gel and quantified with a NanoDrop One© spectrophotometer (ThermoFisher). Double-stranded RNA targeting the gene encoding green fluorescent protein (GFP ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot was boiled at 85°C for 15 min and subjected to a Pierce 660 (Thermo Fisher, Waltham, MA) total protein quantification following manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Sufficient quality and quantity of extracted DNA was assessed using a NanoDrop One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... one with RNAse A treatment (1 µL at 10 mg/mL, ThermoScientific, #EN0531) and one with DNAse treatment (1µL at 2U/µL, Invitrogen, #AM2238). Nucleic acids were separated using denaturing polyacrylamide gel (TBE-Urea gel ...
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bioRxiv - Molecular Biology 2023Quote: The TaqMan assays were performed using a one-step TaqMan probe kit (TaqManTM RNA-to CTTM 1-Step Kit, Invitrogen). As the TaqMan probe we used 12➔7 Probe FAM–ACCATCAGCCCCCTTTTTATTTCCT-MGBNFQ ...
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bioRxiv - Molecular Biology 2023Quote: ... One microgram of total RNA was used for the first strand cDNA synthesis using the Verso cDNA Synthesis Kit (ThermoFisher) with RT-enhancer to remove residual genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: ... the kidneys from one mouse were harvested and minced into small pieces (∼1 mm3) in Hanks’ Balanced Salt Solution (HBSS, #2323615, Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... Digested samples were loaded onto Evotips and washed once with aqueous acidic buffer (0.1% formic acid in water) before loading onto an Evosep One system coupled to an Orbitrap Fusion Lumos (ThermoFisher Scientific). The Evosep One was fitted with a 15 cm column (PepSep ...
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bioRxiv - Molecular Biology 2023Quote: ... or used directly as a template for RT-PCR using SuperScript IV One-Step RT-PCR System (Cat# 12594100, Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted as described above and the region of interest was amplified using specific primer pairs and the SuperScript IV One-Step RT-PCR System (Cat# 12594100, Invitrogen). Amplified region was directly sent for overnight sequencing service at Microsynth AG ...
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bioRxiv - Bioengineering 2024Quote: ... CAR T cells were thawed one day prior to the start of the experiment in RPMI-GlutaMAX 1640 + 25 mM Hepes (ThermoFisher) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Physiology 2024Quote: ... One microliter of 500 µM cell-impermeant calcein (ex./em. maxima of 494/514 nm) (Thermo Fisher Scientific, Waltham, MA) stock solution in dimethyl sulfoxide (DMSO ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The genomic DNA amount and quality were evaluated with NanoDropTM One UV-Vis spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and Qubit ® 1.0 Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... 5’ probes FAM-ACCCCGCATTACGTTTGGTGGACC-QSY 3’) and Quantitative Superscript III Platinum One-step RT-qPCR systems with the ROX kit (Invitrogen), according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: At least 200 million cells (one flash-frozen pellet) per sample was resuspended in 400μL Trizol (Thermo Fisher 15596-026) in a 1.5-ml tube and vortexed with 500μl of glass beads (Sigma G8772 ...
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bioRxiv - Evolutionary Biology 2024Quote: Fragments of Po-exd and Po-N were amplified using standard PCR protocols and cloned using a TOPO TA Cloning Kit using One Shot Top10 chemically competent Escherichia coli (ThermoFisher) following the manufacturer’s protocol ...
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Assessment of the transcriptional regulation of EMT and MET through accessible transposable elementsbioRxiv - Genomics 2024Quote: ... One microgram of total RNA was used for cDNA synthesis using the Maxima First Strand cDNA Synthesis kit (Thermo Scientific). Primers and probes used for qPCR were previously reported (Alotaibi et al ...
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bioRxiv - Plant Biology 2024Quote: ... and one 7 mm surgical cotton ball was placed in each well of the 96 well plates (Thermo Fisher scientific). Black plates were used to minimize light reflection and penetration ...
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bioRxiv - Plant Biology 2024Quote: ... One μg of total RNA was used for each RT reaction implementing Maxima First Strand cDNA Synthesis Kit (ThermoFisher, K1641) and oligodT primers provided with the kit ...
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bioRxiv - Neuroscience 2024Quote: ... were pretreated with or without MaR1 (10 nM) for 30 min and then stimulated with Dynabeads CD3/CD28 T-Cell Expander (one bead per cell; Invitrogen) for 8 h ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was incubated for 30 min at 23 °C and stopped by the addition of one volume of 2x concentrated NuPAGE LDS loading buffer (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... tissues were stained for one and a half hours with Alexa Fluor 568 donkey anti-goat (1:400; A11057, Invitrogen) and DAPI (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... One part of this RNA was reverse-transcribed into cDNA using random hexamer primers and SuperScript III Reverse Transcriptase (Invitrogen), as per the provided guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... One day after infection (day 0), the medium was replaced with F12-N2 (DMEM/F12, 1% N2, 1% NEAA (Gibco)) ...