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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... unprimed cells were transfected with LPS (5 μg/ml) using Lipofectamine 2000 (1% v/w; Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... culture medium was completely aspirated and macrophages were lysed with 5 ml/dish of Trizol (Invitrogen) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... APEX2 ORF was then PCR amplified and inserted into pcDNA™5/FRT Vector (Invitrogen™) using NEBuilder® HiFi DNA Assembly (NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... The eggs were then loaded with the calcium indicator Fura-2 AM (5 μM; Thermo Fisher) for 30 min in KSOM containing 0.02% pluronic F-127 (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes with 5 μM of CellROX Deep Red (Thermo Fisher Scientific #C10422) or CellROX Green (Thermo Fisher Scientific #C10444 ...
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bioRxiv - Genomics 2020Quote: ... and total RNA was extracted from five (n=5) COVID-19 positive patients using TRIzol (Invitrogen) reagent following manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... cDNA was then quantified utilizing Taqman Q-PCR probes (QuantStudio 5 Real-Time PCR System, ThermoFisher). Data was then analyzed using Microsoft Excel and plotted in GraphPad PRISM.
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bioRxiv - Neuroscience 2022Quote: ... Sequence PCR (total volume 5 µl) was carried out with 1 µl Big dye (Applied Biosystems), 1 µl Forward primer ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative Real Time PCR assays were carried out by using QuantStudio 5 (Thermo Fisher Scientific, Germany) and GoTaq®aPCR Master Mix with SYBR green fluorescence (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... actin polymerization was inhibited by pretreating cells with 5 μM Cytochalasin D (Invitrogen; Cat. No. PHZ1063) for 30 min before the addition of phagocytic substrate media ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were manually triturated in cell medium (DMEM + GlutaMAX with 5% horse serum, Thermo fisher, 26050-070 and 0.5% Penicillin-Streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... the drinking water contained thymidine analogue EdU (5-ethynyl-2’-deoxyuridine, Thermo Fisher, cat. no: E10415) to label newly-generated cells ...
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bioRxiv - Neuroscience 2021Quote: ... and cultured at 35°C with 5% CO2 in 7.4 g/L MEM (ThermoFisher, #11700-077) with the addition of (in mM unless labeled otherwise) ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated at 37°C in humidified 5% CO2 incubators (Eppendorf, GmBH or Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2020Quote: ... lysates (~5 × 106 cells per experiment) were pre-cleared with PierceTM Protein A Agarose (Thermo Scientific) for 2 hr at 4°C ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... the Click-iT EdU (5-ethynyl-2’-deoxyuridine) Alexa Fluor (AF) 568 imaging kit (Molecular Probes) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... male) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with 10% fetal bovine serum (EUROBIO ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% sodium dodecyl sulfate (SDS) in 50 mM triethylammonium bicarbonate (TEAB, Thermo Fisher Scientific, Rockford, IL), pH=8.0) ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 μl of 5 M NaCl and 10 μl of 10 mg/ml proteinase K (Invitrogen) was then added to the input ...
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bioRxiv - Microbiology 2021Quote: Peptides were labelled with Alexa Fluor® 488 (A488) 5-sulfodichlorophenol ester (SDP) (Thermo Fisher Scientific) using previously described methods [66] ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl RNase A/T1 mix (2 μg μl−1 / 5 U μl−1, Thermo Scientific) was added to 100 μl lysate (OD260 ~ 150 ...
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bioRxiv - Genomics 2020Quote: ... The 5’ end cap structure was biotinylated and captured with streptavidin-coated magnetic beads (Thermo Fisher). After ligation of 5’ and 3’ adaptors ...
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bioRxiv - Immunology 2021Quote: ... Plates were developed by adding 50 μl TMB substrate for 5 min with shaking (Thermo Fisher), followed by 50 μl TMB stop solution (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: Human erythroleukemia K562 cells were grown at 37°C at 5% CO2 in RPMI 1640 (Gibco) with 10% FBS (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 mM NaCl and 78 mM MgCl2) and stained with 5 μg/mL propidium iodide (Invitrogen) in FACS buffer ...
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bioRxiv - Bioengineering 2019Quote: Template DNA was amplified using 0.5 μL of Taq DNA polymerase (5 units/μL, Invitrogen, 100021276) in a 50 μL reaction containing 1X Taq polymerase Rxn Buffer (Invitrogen ...
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Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cyclebioRxiv - Evolutionary Biology 2019Quote: ... The reaction mixture consisted of 5 µL of SYBR Green MasterMix (Applied Biosystems, Carlsbad, California, USA), 0.3 µL of 10 µM primers (Table S3 ...
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bioRxiv - Immunology 2020Quote: ... Sample (5 μL) was injected onto a C18 packed-tip column (75μm x 100mm, Thermo Scientific) at 55°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and an aliquot of 5 μL was loaded onto a 15% polyacrylamide TBE-Urea gel (Invitrogen). The gel was run at 180 volts for 75 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... The disaggregation of ICM was performed with 5 min of 0.05% trypsin-EDTA (GIBCO, 25300-054) treatment and the cell suspension put on culture in ES derivation medium on MEFs ...
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bioRxiv - Molecular Biology 2019Quote: ... We added this sequence to the 5’-end of the arrO gene through GeneArt (ThermoFisher Scientific) and incorporated this arrO-N sequence into the DinoIII vector (DinoIII-arrO-N ...
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bioRxiv - Microbiology 2019Quote: ... HEp-2 cells were initially seeded in growth medium (DMEM medium with 5% FBS (ThermoFisher, USA), 1% Penicillin/Streptomycin (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... EGF stimulation was performed for 5 minutes with 100 ng/µl of epidermal growth factor (Gibco) at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... Nucleotide substrates were purchased from: ATP (Adenosine 5′-triphosphate Disodium Salt Trihydrate, Fisher Scientific BP413-25) GTP (Guanosine 5′-triphosphate sodium salt hydrate ≥95% ...
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bioRxiv - Neuroscience 2019Quote: HEK T293 cells were cultured at 37°C in 5% CO2 in DMEM+GlutaMax (Thermo Fisher) with 10% fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... The cells were stimulated using plate-bound anti-CD3 (5 µg/mL; eBioscience, Thermo Fisher Scientific) and soluble anti-CD28 (1 µg/mL ...
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bioRxiv - Genetics 2019Quote: ... the oligonucleotide probes were 5’ end labeled with [γ-32P]ATP using polynucleotide kinase (Thermo Scientific), purified through Sephadex G-50 chromatography ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µg of the respective antibodies (Anti-ERα; ThermoFisher MA5-13065 or Anti-Flag; Sigma F3165) were added to the lysate and incubated on a rotating wheel at 4°C overnight.
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bioRxiv - Neuroscience 2019Quote: ... Cells were grown at 35 °C and 5% CO2 in DMEM with GlutaMAX (Thermo Scientific, #10569010) supplemented with 5% heat-inactivated FBS (Thermo Scientific #10082147 ...
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bioRxiv - Biochemistry 2019Quote: ... 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA; Molecular Probes, Eugene, OR, USA) was used to detect NO production in sorghum genotype stalks in response to inoculation treatment at 7 DPI ...
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bioRxiv - Microbiology 2019Quote: ... and anti-Foxp3 (FJK-16s) and anti-CD4 (RM4-5) antibodies were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were grown at 35 °C and 5% CO2 in DMEM with GlutaMAX (Thermo Scientific, #10569010) supplemented with 5% heat-inactivated FBS (Thermo Scientific #10082147 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected for 5 hours with 500 ng CD31 plasmid and 0.3% Lipofectamine 2000 (Invitrogen). After 48 hr selection with 5 μg/ml blasticidin began ...
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bioRxiv - Neuroscience 2019Quote: ... 5% CO2 and 37°C in neuronal culture medium (Gibco Neurobasal medium supplemented with 1x Gibco B-27 supplement ...
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bioRxiv - Developmental Biology 2019Quote: ... 10μl of transfection mix [12.5μg siRNA in 100μl 5% glucose and 1.5μl TurbofectTM (Thermo Fisher Scientific)] in 490μl of DMEM (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... Samples were loaded onto a trapping column (Thermo Scientific, PepMap100, C18, 300 μm X 5 mm), using partial loop injection ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1.5 min followed by three 5 second washes in 95% Reagent Alcohol (Thermo Fisher, Histoprep), two 5 second washes in 100% Reagent Alcohol (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... incubated in 500 uL of PBS-0.5% BSA containing 5 ug/mL RNAse A (Thermo Scientific) and 1 ug/mL DAPI (#564907 ...
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bioRxiv - Cancer Biology 2019Quote: ... The digested antibody was loaded onto a 5 ml CaptureSelect FcXL column (ThermoFisher Scientific, Waltham, MA), equilibrated with 1x PBS and the Fab collected in the flow through ...
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bioRxiv - Immunology 2019Quote: ... Excised lungs were places in DMEM substituted with 5% FBS and 1% Penicillin/Streptomycin (all Gibco). Lungs were immediately sliced into 300μm thick sections on a vibratome and stained with directly conjugated Ab in complete medium (phenol-red free DMEM substituted with 10% FBS ...