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Citations for Thermo Fisher :
6001 - 6050 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Primary human parenchymal lung fibroblasts from non-COPD and COPD patients (n=8) were grown in DMEM (Gibco) supplemented with 5% FBS and 2% antibiotics at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: Human HAP1 derivative reporter cell line (HAP1-AAVS-EGFP, Tan et al., 2022) were cultured in IMDM (Gibco) supplemented with 10% FBS (Corning ...
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bioRxiv - Genetics 2023Quote: ... the pseudo- haploid calling was carried out on the 593,124 autosomal sites on the Human Origins Array (Affymetrix). Contamination was estimated based on the X-chromosome and mitochondrial DNA using ANGSD(86 ...
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bioRxiv - Bioengineering 2023Quote: Human lung squamous cell carcinoma cell line NCI-H226 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human Osteosarcoma cell line SJSA-1 (formerly OsA-CL) was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human lung squamous cell carcinoma cell line NCI-H1975 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human Osteosarcoma cell line SJSA-1 (formerly OsA-CL) was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: CEM were counted and resuspended at a concentration of 2-3 x 106 cells/mL in ice cold FACS buffer (PBS, 10% FCS, 2mM EDTA) containing 1.25 µL anti-human CD71 (0.625 µg/mL, OKT9, Invitrogen) for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck) ...
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bioRxiv - Neuroscience 2023Quote: Total tau content was quantified in brain extracts by ELISA (Human Tau (total) ELISA kit (#KHB0041, Invitrogen, UK), see supplementary methods ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human embryonic kidney cell lines (HEK293) containing a Flp-In expression vector (HEK293 Flp-In) obtained from ThermoFisher Scientific were utilized in this study ...
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bioRxiv - Microbiology 2023Quote: ... The human KIF1A coding sequence (DNASU Plasmid Repository, #HsCD00829423, NCBI Nucleotide reference NM_001244008.2) was inserted by Gateway recombination (Invitrogen) to make an in-frame KIF1A-EmGFP fusion.
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bioRxiv - Neuroscience 2023Quote: ... HPCs in suspension were transferred to plates containing an adherent layer of confluent primary human astrocytes (ThermoFisher, N7805100), in media containing Iscove’s Modified Dulbecco’s Medium (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Human cMyc-mGlu1 cDNA sequence was subsequently transferred from pcDNA3.1(+) into the destination vector pcDNA5/FRT/TO (Invitrogen) using NEBuilder HiFi assembly (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... The human cutaneous T-cell lymphoma cell line HUT78 was cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco) with 20% FBS and penicillin-streptomycin 100X ...
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bioRxiv - Immunology 2023Quote: ... candidate regulatory elements (RE) were PCR amplified from human or mouse genomic DNAwith Accuprime Pfx DNA polymerase (Invitrogen) using primers with a NheI or XhoI cut site added to their 5′ end (primer sequences available in Table S3) ...
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bioRxiv - Bioengineering 2023Quote: Cell culture medium Essential 8 (E8) Flex Medium and recombinant human vitronectin (rhVTN-N) were purchased from Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Human embryonic kidney cells (HEK293T) were cultured in 10% DMEM (90% DMEM high glucose with GlutaMAX (Gibco, 10569010), 10% FBS (GE ...
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bioRxiv - Microbiology 2023Quote: ... 30 nM of siRNA was transfected into the human macrophages using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Human monocyte cell line THP-1 cells were cultured in RPMI 1640 medium with added 10% FBS (Gibco) and 1% penicillin-streptomycin (Hyclone) ...
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bioRxiv - Bioengineering 2023Quote: Human lung squamous cell carcinoma cell line NCI-H1975 was maintained in RPMI-1640 (GIBCO, Thermo Fisher Scientific) plus 10% fetal bovine serum (GIBCO ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma gondii strain RH (expressing YFP off of the GRA1 promoter) (18) was cultured in human foreskin fibroblast (HFF) in complete Dulbeccòs modified Eagle’s medium (cDMEM; ThermoFisher Scientific plus 100 U/ml penicillin/streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... We maintained K562 cells (human chronic myelogenous leukemia, ATCC CCL-243) in RPMI 1640 medium (Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293) cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2022Quote: ... The resulting monocytes were then differentiated with 50 ng/ml human M-CSF (Miltenyi) for 6 days in Iscove’s modified Dulbecco’s medium (Gibco) containing 5 % fetal calf serum (CAPRICORN ...
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bioRxiv - Immunology 2023Quote: ... DC:T cell co-cultures were performed in the presence of soluble anti-human CD3 (16-0037-85, ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: MS/MS spectra were searched against human database from Uniprot (downloaded on 04/2015) with Proteome Discoverer 2.1.1.21 (ThermoFisher Scientific). Methionine oxidation (+15.9949 Da) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA extraction was performed from the medial layer of the aorta and subsequently sequenced by HTA 2.0 (Human Transcriptome Array 2.0 - Affymetrix) platform.
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bioRxiv - Cell Biology 2023Quote: ... 0.055 mM β-mercaptoethanol and 10 ng/ml recombinant human full-length bFGF (All reagents from Gibco, USA)].
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bioRxiv - Developmental Biology 2023Quote: Human ESCs were thawed and maintained on mitotically inactivated MEFs in Knock-Out DMEM media (Thermo Fisher Scientific) supplemented with 20% Knock-Out Serum Replacement (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... Human GM12878 lymphoblastoid cell line (Coriell Institute) which has a relatively normal karyotype was cultured in RPMI (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Cell Biology 2023Quote: Human colon adenocarcinoma HT-29 cells were routinely cultured in Dulbecco’s Modified Eagle’s Medium (Cellutron Life Technologies, USA) with a high glucose concentration ...
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bioRxiv - Bioengineering 2023Quote: ... The plasma insulin concentration was determined using a Human Insulin ELISA kit according to the manufacturer’s protocol (Invitrogen). The relative bioavailability (RA ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
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bioRxiv - Cancer Biology 2023Quote: Human osteosarcoma cell line U2OS (ATCC) and COV362 (ATCC) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM; Gibco) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: Human umbilical vein endothelial cells (HUVECs) were isolated and cultured in an M199 medium (Thermo Fisher Scientific, 31100035) supplemented with fibroblast growth factor ...
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bioRxiv - Molecular Biology 2023Quote: ... The human cell lines were cultured in DMEM/High glucose medium (Cat # SH30243.02, Thermo Fisher Scientific, Logan, UT) supplemented with 10% FBS (Cat # F2442 ...
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bioRxiv - Microbiology 2022Quote: ... TNF-alpha treatments were performed for 45 min using 25 ng/ml of human TNF-alpha (Thermo Fisher). Cells were permeabilized with 0.1% Triton X in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: Human RPE-1 cells were cultured in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F- 12 (DMEM/F12) (GIBCO), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Combined libraries were dried down with universal blockers and human cot DNA in a Savant SpeedVac (ThermoFisher Scientific) vacuum concentrator ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Neuroscience 2024Quote: Human CSF2RB WT (exons 11 to 14) and A455D mutant donor targeting sequences were cloned into pDONR221 (ThermoFisher) with the following layout (Supplementary Figure S1) ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco by Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco by Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2023Quote: ... Huh7 (Human hepatocellular carcinoma) cells were grown in DMEM (Dulbecco’s Modified Eagle Medium) (Gibco; 11885-084; Low glucose), and HepG2 (Human hepatocellular carcinoma ...
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bioRxiv - Immunology 2023Quote: ... human samples were stained with eBioscience™ Fixability Viability Dye eFluor™ 780 (65-0865-18, ThermoFisher, Canada), while murine samples were stained with Zombie Green™ Fixable Viability Dye (423111) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the top 14-HAP depleted and fractionated sample were searched for peptides through the human UniProtKB/SwissProt database (v2021-11-30, 20,306 canonical sequences) using Proteome Discoverer (version 2.5.0.400, Thermo Fisher Scientific). The parameters applied to the search engines Sequest HT and Mascot were set as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... MCF10A (mammary gland epithelial; human female origin, ATCC #CRL-10317) cells were maintained in DMEM/F12 (Life Technologies) supplemented with 5% horse serum ...