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Citations for Thermo Fisher :
5901 - 5950 of 7821 citations for HEPACAM Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... hEPSCs were grown using ‘human Expanded Potential’ (hEP) medium consisting of DMEM/F12 (Thermo Fisher Scientific, 11320-033), Neurobasal-A (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Tetramers were mixed with the following anti-human antibody cocktail: anti-CD16 APC-eFluor780 (Invitrogen, #47-0168-41), anti-CD8α APC-eFluor780 (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... parasites were cultured in human red blood cells (RBC) in RPMI-1640 culture medium (Thermo Fisher # 53400-025) supplemented with 10% v/v Albumax I (Thermo Fisher # 11020039) ...
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bioRxiv - Cancer Biology 2020Quote: ... and human embryonic kidney epithelial cell line (HEK293T) were maintained at 37°C in RPMI 1640 (Life Technologies) and DMEM supplemented with 10% fetal bovine serum and Penicillin and Streptomycin (1mM) ...
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bioRxiv - Molecular Biology 2020Quote: SNP 6.0 analysis was performed by Aros AB (Denmark) using a Genome-Wide Human SNP Array 6.0 (Affymetrix) on RPE1 cells and two edited RPE1 lines derived from the parental RPE1 line ...
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bioRxiv - Evolutionary Biology 2020Quote: Cells in 6-well plates were transfected with 1 ug human or cow CD44-promoter-pNL2.1 or empty vector pNL2.1 using Lipofectamine 3000 (Invitrogen) as manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was extracted from both medial and adventitia layer of the aortic wall and subsequently sequenced on HTA 2.0 (Human Transcriptome Array 2.0 - Affymetrix) platform.
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bioRxiv - Neuroscience 2021Quote: ... 200 µg of human serum) were separated by SDS-PAGE on 3-8% tris-acetate gels (NuPAGE, Invitrogen) under reducing conditions at 150 V (cellular fraction ...
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bioRxiv - Cancer Biology 2021Quote: Cells were stimulated for a total of 6 hours with human T-activator anti-CD3/CD28 Dynabeads (ThermoFisher) stimulation with GolgiStop solution being added for a total of 5 hours block intra-cellular protein transport (BD Bioscience) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...
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bioRxiv - Synthetic Biology 2021Quote: BMP4 concentration released into the media was quantified using the BMP-4 Human ELISA Kit from (ThermoFisher, EHBMP4). Standards and protocols were done according to the instructions of the kit ...
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bioRxiv - Immunology 2021Quote: ... and anti-Spike IgG was quantified using a Human SARS-CoV-2 Spike (Trimer) IgG ELISA Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Human retinal pigment epithelial ARPE-19 cells (ATCC® CRL-2302™) were cultivated in DMEM/F12 (Gibco) with 10% fetal bovine serum (Lonza) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Addgene No. 154106) are previously described. Human codon-optimized S (GenBank No. YP_009724390.1) was subcloned into pCEP4 (Invitrogen) from pUC57-2019-nCoV-S(Human ...
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bioRxiv - Cell Biology 2020Quote: ... anti-human Ig Alexa Fluor 488 (A11013) or anti-rabbit Ig Alexa Fluor 568 (A11036; Thermo Fisher Scientific), according to primary antibody species.
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bioRxiv - Cell Biology 2020Quote: The human bronchial epithelial cell (HBEC6-KT) line was cultured in Keratinocyte Serum-Free Medium 1X (Gibco®) according to the manufacturers directions and supplemented with P/S (VWR® ...
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bioRxiv - Cancer Biology 2021Quote: ... Monoclonal human osteosarcoma cell (U2OS 3XFlag-HaloTag-Atg9A, see below) were cultured in RPMI media (Gibco/ThermoFisher, A4192301) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: ... Monoclonal human osteosarcoma cell (U2OS 3XFlag-HaloTag-Atg9A, see below) were cultured in RPMI media (Gibco/ThermoFisher, A4192301) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with FACS buffer and stained with goat anti-Human IgG Alexa Fluor 647 (Invitrogen) or goat anti-Mouse IgG Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed using buffer sets of Human Regulatory T-cell Staining Kit (eBioscience/Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and TLR4 on the beads were stained with mouse monoclonal APC anti-human TLR4 antibody (clone HTA125; ThermoFisher) for 1 h on ice prior to flow cytometric analysis ...
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bioRxiv - Immunology 2020Quote: ... 1 mM EDTA.) with the following anti human antibodies: anti CD3-APC-eFluor 780 (Invitrogen, 47-0037-41), anti CD14-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: SV40-transformed normal (MRC5-V1) and XPV (XP30RO) human fibroblasts were grown in Minimum Eagle Medium (MEM, Gibco) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2021Quote: The human hepatocellular carcinoma Huh7 cell line (CLS 300156) was cultured in Dulbecco’s modified eagle medium (DMEM, Gibco) with glutamax supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Labelled cDNA was hybridised to an Affymetrix GeneChip® Human Transcriptome Array 2.0 (Applied Biosystems, Thermo Fisher Scientific). Raw intensity files (CEL files ...
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bioRxiv - Immunology 2020Quote: ... Labelled cDNA was hybridised to an Affymetrix GeneChip® Human Transcriptome Array 2.0 (Applied Biosystems, Thermo Fisher Scientific). Raw intensity files (CEL files ...
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bioRxiv - Microbiology 2021Quote: Human primary fibroblasts (HFFs, ATCC® CCL-171™) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% heat inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Positive proliferation control wells were set up by plating 1 Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) for every 200 T-cells ...
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bioRxiv - Immunology 2021Quote: Isolation of CD3+ T-cells was performed using Dynabeads® Untouched™ Human T-cells Kit (11344D, Invitrogen). CD3+ T-cells were stained with CellTrace Violet Cell Proliferation Kit (C34571 ...
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bioRxiv - Immunology 2020Quote: ... BM14 (ECACC 88052033) human HLA-Typed Lymphoblastoid Cell Line was obtained from ECACC and maintained in RPMI (ThermoFisher) media supplemented with 10% FBS in the absence of antibiotics ...
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bioRxiv - Immunology 2021Quote: ... The human multiple myeloma cell line XG-1 was cultured in Iscove modified Dulbecco medium (Invitrogen Life Technologies) containing 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... The human multiple myeloma cell line XG-1 was cultured in Iscove modified Dulbecco medium (Invitrogen Life Technologies) containing 10% fetal bovine serum ...
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bioRxiv - Genomics 2021Quote: ... Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher, #A1049101) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated using a 1:1 ratio of DynaBeads Human T-Activator CD3/CD28 (Thermo Fisher) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Human foreskin fibroblast (HFF) cells were grown and maintained in DMEM containing 4.5g/L glucose (Gibco 11965-092), supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: HEK293F is a female human embryonic kidney cell line transformed and adapted to grow in suspension (Life Technologies). HEK293F cells were grown in 293FreeStyle expression medium (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Immunology 2022Quote: ... Human Raji (Burkitt’s lymphoma) and SKOV3 (ovarian carcinoma) were cultured in complete RMPI (Roswell Park Memorial Institute, Gibco) and DMEM (Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Immunology 2022Quote: ... podocytes were treated with patient sera in the presence of a control antibody (human IgG, Invitrogen #02-7102) or anti uPAR antibody (2G10 ...
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bioRxiv - Biophysics 2022Quote: ... Poly-L-lysine coated chambers were then incubated with anti-human CD3 antibody (HIT3a clone, Thermo Fisher Scientific) in PBS at a concentration of 10 µg/mL for 2 hours at 37°C or overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells and MDA-MB-231 human breast cancer cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) with reduced glucose (1 g/L) ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs using the Dynabeads® Untouched Human T Cells Kit (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... labeled with Alexa Fluor 488 from the Zenon™ Human IgG Labeling kit (Molecular Probes, Eugene, OR, USA) and 15 μg/mL of 7-amino-actinomycin D (7-AAD ...
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bioRxiv - Biophysics 2022Quote: ... designated as ‘Cav1.2(ΔC)’) construct was obtained from a full-length human Cav1.2 α1 (Uniprot Q13936-20) template in pcDNA3.1 (Invitrogen) using a Gibson Assembly kit (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... Histidine rich glycoprotein (HRG) was purified from human plasma with a cobalt-loaded resin (Thermo Scientific, Waltham, America) using IMAC-based enrichment (56) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 μl each) using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (ThermoFisher, Catalog number: EPX650-16500-901), as per manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and aged (>60 years) human primary keratinocytes (HPK) were isolated with trypsin (Gibco, Life Technologies, Carlsbad, CA, USA) and dispase (Dispase II ...
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bioRxiv - Cell Biology 2022Quote: ... and aged (>60 years) human primary keratinocytes (HPK) were isolated with trypsin (Gibco, Life Technologies, Carlsbad, CA, USA) and dispase (Dispase II ...
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bioRxiv - Cell Biology 2022Quote: Human acute monocytic leukemia THP-1 (ATCC, TIB-202™) cells were cultured in RPMI-1640 medium (Gibco) supplemented with heat-inactivated 10% fetal bovine serum (FBS ...