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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The GST- fusion proteins were purified under native conditions using glutathione agarose (ThermoFisher Scientific) and the LRRCC1 fragments were recovered by Tev protease cleavage and dialyzed before rabbit immunization (Covalab) ...
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bioRxiv - Molecular Biology 2019Quote: 6xHis 12-heptad SRS fusion proteins were expressed in SoluBL21 Escherichia coli cells (Invitrogen) from a pet42a vector ...
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bioRxiv - Bioengineering 2021Quote: Fusion proteins were purified using Ni-NTA resin according to manufacturer’s instructions (Thermofisher, R90115). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... 250 μg of fusion proteins were mixed to 50 μg of Biotin-NHS (Invitrogen) in a total volume of 1 mL ...
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bioRxiv - Genetics 2022Quote: ... while cellular protein fractions were analyzed on an Orbitrap Fusion mass spectrometer (Thermo Fisher). The 6-plex Tandem Mass Tag (TMT ...
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bioRxiv - Molecular Biology 2019Quote: ... The Cas12a fusion protein was expressed in BL21 star (DE3) cells (Thermo Fisher Scientific) using a previously established protocol with minor modifications (Strohkendl et al. ...
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bioRxiv - Microbiology 2023Quote: Protein identification by LC‒MS/MS was conducted on Orbitrap Fusion Lumos (Thermo Fisher) by Shandong University at core facilities for life and environmental sciences ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were then incubated using FITC-labelled goat-anti-human IgG-FC antibody (ThermoFisher Scientific) (200 ng/ul ...
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bioRxiv - Microbiology 2021Quote: ... R-phycoerythrin (PE) conjugated goat anti-human IgG Fc secondary antibody (Invitrogen, Cat#12-4998-82) and PE conjugated mouse anti-PSGL-1 mAb (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... competition was detected by HRP-goat anti-human IgG Fc secondary antibody (A18817, Thermo Fisher Scientific). The kinetics of the antibody fragments were determined using a Biacore X100 (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc or DC-SIGN-Fc (Thermofisher) in binding buffer (BB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by binding of antigen antibody complexes to protein A/G magnetic beads (Thermo scientific, Rockford, USA) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... Protein antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). OSP and LPS antigens were modified by 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium and conjugated to Luminex Magplex carboxylated beads ...
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bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant proteins were labeled with Alexa Fluor(tm) 647 NHS ester (Thermo Fisher) as described previously18 ...
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bioRxiv - Biochemistry 2022Quote: ... the recombinant protein mixtures were combined with 12.5 μM diFMUP (Life Technologies, E12020), added to 384-well plates ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... the recombinant proteins were dialyzed using Slide-A-Lyzer™ cassettes (Thermo Fisher) into cold coupling buffer containing 100mM NaHCO3 and 500mM NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... Recombinant protein expression was triggered using 0.5mM isopropyl β-D-1-thiogalactopyranoside (ThermoFisher) and incubated for 18 h in an orbital shaker at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant Spike protein was labelled with Alexa Fluor™ 546 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Pathology 2022Quote: ... and incubated with PierceTM Recombinant Protein L Biotinylated (60µL per kidney, ThermoFisher #29997) in a tube rotator (25min. ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant N protein was purified using Ni2+ resin (Thermo Fisher Scientific, USA). An indirect ELISA assay was conducted with the recombinant N protein as the coating antigen (3 ng/μl ...
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bioRxiv - Cancer Biology 2022Quote: ... and purified recombinant Cas9 proteins (250 ng/ml: Thermo Fisher Scientific, MA, USA). The gRNA sequences are as follows:
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bioRxiv - Cell Biology 2019Quote: Mouse TGF-β 1 recombinant protein was obtained from Affymetrix (#14-8342-62) and used at the concentration of 2 ng/ml unless stated otherwise ...
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bioRxiv - Cell Biology 2020Quote: ... His6-tagged recombinant proteins were produced in BL21 (DE3) grown in MagicMedia (Invitrogen) 24h ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... The samples were mixed with recombinant protein G-Sepharose 4B beads (Thermo Fisher) and 1 µl anti-Flag antibodies (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific). On-bead recombinant proteins were washed with the respective lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Biochemistry 2021Quote: Recombinant proteins were labeled with Alexa Fluor™ 647 NHS ester (Thermo Fisher) as described previously.20 In brief ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM Pierce™ recombinant protein A (#21184, Thermo Fisher Scientific, Waltham, USA) 50 nM bovine serum albumin (#A4612 ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
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bioRxiv - Immunology 2020Quote: ... Both RSV strains were propagated on HEp-2 cells (ATCC, CCL-23) cultured in DMEM (Gibco) supplemented with 5% FBS (Gibco) ...
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bioRxiv - Biochemistry 2023Quote: Insect cell derived recombinant tag-less eIF2A was expressed in ExpiSf9 cells using the ExpiSf Expression System Starter Kit (Thermo Fisher # A38841) following the manufacturer’s protocol and adapting the IMPACT (Intein Mediated Purification with an Affinity Chitin-binding Tag ...
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bioRxiv - Microbiology 2024Quote: ... polygyrus E/S-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugate (Invitrogen, cat no. A10551).
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bioRxiv - Cancer Biology 2023Quote: ... Respiratory chain complexes and supercomplexes were separated on Native PAGE Novex 3%–12% Bis-Tris gels (Invitrogen) for approximatively 3 h and transferred to polyvinylidene fluoride membranes (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Molecular Biology 2021Quote: ... Fab and Fc regions were separated using a Nab Protein A column (Thermo Scientific). Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13 was purified with Protein A agarose resin (Thermo Fisher Scientific; 20334) according to the manufacturer’s instructions with the exception that all buffers were supplemented with 1tab/50mL of cOmplete Protease Inhibitor Cocktail and 10uM Pepstatin A ...
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bioRxiv - Cancer Biology 2019Quote: ... iPSC line WTC-11 (Coriell Institute) was maintained on recombinant human vitronectin[36,37](Thermo Fisher Scientific), coating six-well tissue culture plastic plates (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Microbiology 2020Quote: Mice engrafted with J-Lat cells were treated with recombinant human TNF-α (Cat. #PHC3011, Gibco), a potent and well-known LRA ...