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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody used was R-Phycoerythrin labelled Goat anti-Human IgG Fc (eBioscienceTM; 12-4998-82, Invitrogen).
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bioRxiv - Microbiology 2023Quote: Human pharyngeal carcinoma Detroit 562 epithelial cells (ATCC_CCL-138) were grown in 10% FCS in alpha MEM media (Gibco). For each set of experiments ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Immunology 2024Quote: ... antigen proteins of the target were biotinylated using the EZ-link™Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher), then coupled to the fluorescent neutravidin beads (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... containing N-terminal ten histidine tag (His-tag) and human rhinovirus 3C (HRV 3C) protease site was amplified by PCR and cloned into the pFastBac vector (Invitrogen) using seamless cloning method ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Detection of EDA positive fibronectin was performed using biotinylated fusion proteins followed by Streptavidin Alexa 488 (Invitrogen). In parallel the neovasculature marker CD31 was detected using either rat anti-mouse CD31 (BD Pharmingen ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were transiently transfected with the corresponding fusion protein cDNA using Lipofectamine 3000 (Invitrogen, Paisley, Scotland, UK).
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bioRxiv - Plant Biology 2020Quote: ... The GST and GST-XIK fusion proteins were extracted with Pierce Immobilized Glutathione resin (Thermo Fisher Scientific) with the protein interaction buffer (20 mM Hepes-KOH pH7.2 ...
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bioRxiv - Immunology 2022Quote: For purification of the fusion proteins by His Pur™ Ni-NTA Resin (Thermo Scientific, Beijing, USA), the 250 mL IPTG-induced bacterial solution was centrifuged at 4 °C at 12000 rpm for 10 minutes ...
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bioRxiv - Plant Biology 2019Quote: ... Constructs for CRK2–YFP and YFP–Myc fusion proteins were created using the MultiSite Gateway technology (Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The SALM3 fusion protein expression in transfected cell was detected with Floid™ Cell Imaging system (ThermoFisher) using immunofluorescence.
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bioRxiv - Cell Biology 2022Quote: ... MBP fusion proteins were eluted in MBP buffer supplemented with 10 mM maltose (Fisher Scientific; M75-100) and GST proteins were eluted in GST elution buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell expressing protein fragments with an MBP tag were lysed first using 1 mL Bacterial Protein Extraction Reagent (B-PER; Thermo Scientific Catalog number: 78243) and 1-2x of protease inhibitor (Thermo Scientific A32963) ...
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bioRxiv - Biochemistry 2022Quote: ... followed by C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For SARS-CoV-2 RBD VOCs proteins ...
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bioRxiv - Immunology 2022Quote: ... followed by a foldon trimerization motif and C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For competition ELISA experiments ...
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with pcineo plasmids (0.4 μg) carrying the RSV codon-optimised NS1 or FLAG-NS1 WT or mutant constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... with each of the three injections consisting of 10 µg for RSV-F and 1% Alum (Thermo Scientific, 77161) adjuvant ...
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bioRxiv - Biochemistry 2023Quote: ... carrying the RSV A2 WT or T205D M along with pcDNA3.1 codon-optimized plasmids carrying RSV A2 P and F using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... and goat anti-mouse IgG1 Alexa 547 (Invitrogen, A21123) (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Anti β-actin (mouse IgG1) antibody (Thermo Fisher Scientific) used for staining of primary ...
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bioRxiv - Cell Biology 2022Quote: ... and goat anti-mouse IgG1 Alexa 547 (Invitrogen, A21123) (1:100 ...
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bioRxiv - Bioengineering 2019Quote: ... or a control isotype antibody (IgG1 kappa, Thermo Fisher).
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bioRxiv - Neuroscience 2020Quote: ... mouse IgG1 anti-ZO1 (Invitrogen, 33-9100, 1:350), mouse IgG2a anti-V5 peptide (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... AF-conjugated goat anti-mouse IgG1 (Thermo Fisher Scientific), AF-conjugated goat anti-mouse IgG2a (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 555 anti-Mouse IgG1 (Invitrogen; A-21127), and Alexa Fluor 488 anti-Mouse IgG2b (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 555 anti-Mouse IgG1 (Invitrogen; A-21127), and Alexa Fluor 488 anti-Mouse IgG2b (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse IgG1 AlexaFluor 647 (Life Technologies, A21240), goat-anti-guinea pig AlexaFluor 633 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor® 488 anti-mouse IgG1 (A21121, ThermoFisher); Alexa Fluor 568® anti-mouse IgG2b (A21144 ...
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bioRxiv - Cell Biology 2022Quote: ... using GAPDH (mouse monoclonal IgG1, 1:5,000; Thermo Fisher) as housekeeping control ...
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bioRxiv - Neuroscience 2022Quote: ... mouse IgG1 anti-ZO1 (Invitrogen, 33–9100, 1:350). Compatible near-infrared secondary antibodies were used for visualization with the Odyssey system (LI-COR).
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bioRxiv - Bioengineering 2019Quote: ... Mouse IgG1 anti-cardiac troponin (Thermofisher MS-295-P) was used at a dilution of 1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... and Alexa fluor 555 (Invitrogen, goat anti mouse, IgG1). Nuclei were shown by DAPI (4’,6-diamidino-2-phenylindole ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-mouse IgG1 Alexafluor488 (1:500, Life Technologies), goat anti-mouse IgG Alexafluor555 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG1 647 (Life Technologies A21240, 1:500), anti-mouse IgG2A 488 (Life Technologies A21131 ...
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bioRxiv - Neuroscience 2022Quote: ... mouse IgG1 anti-ZO1 (Invitrogen, 33–9100, 1:350), mouse IgG2a anti-V5 peptide (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... mouse IgG1 anti-HA-594 (1:500, Life Technologies), and Alexa 350 ...
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bioRxiv - Microbiology 2023Quote: ... Goat anti-mouse IgG1 Alexa Fluor 488 (Thermo Scientific), Goat anti-mouse IgM Alexa Fluor 488 (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... mouse IgG1 anti-ZO1 (Invitrogen, 33–9100, 1:350), mouse IgG2a anti-V5 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... conjugated with a mouse monoclonal IgG1 isotype antibody (Invitrogen) overnight at 4 ℃ in constant rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa fluor 568 goat anti-mouse IgG1 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... anti-TfR (mouse IgG1, Invitrogen #13-6800, 1:1000); anti-GADPH (mouse IgG1 ...
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bioRxiv - Cell Biology 2024Quote: ... coupled to a control IgG1 mouse immunoglobulin (Thermo Fisher, 14-4714-82 ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-mouse-IgG1-Alexa555 (RRID:AB_2535769, A21127, Invitrogen, 1:800) and anti-rabbit-A488 (RRID:AB_2576217 ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.