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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA). The incubator condition was set at 37 °C with 5 % carbon dioxide environment ...
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bioRxiv - Cell Biology 2023Quote: ... human recombinant fibroblast growth factor 2 (FGF2, 20 ng/mL, Life Technologies), and beta-mercaptoethanol (0.1% ...
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bioRxiv - Cancer Biology 2023Quote: ... bovine pituitary extract and human recombinant epidermal growth factor (Gibco, 37000-015)) and primary gingival keratinocyte (Dermal cell basal medium (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were cultured on human recombinant vitronectin in StemFlexTM media (Life Technologies) and differentiation was initiated by plating singularised hiPSCs on human embryonic stem cells (hESC)-qualified Matrigel (Corning ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human IFN-β (1000 U/ml to 1 U/ml, ThermoFisher) was used as positive controls for IRF activation ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc and DC-SIGN-Fc and then stained with Alexa Fluor® 488 goat anti-human IgG antibody (Life Technologies) diluted 1/200 in PBS + 1% FCS or BB buffer and incubated 30 min on ice ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant P0 virus for each gene were generated in ExpiSf9 insect cells following the manufacturer’s recommendations (Thermo Fisher). The amount of each virus needed for infection has been optimized for maximal production of the two proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated with both WA2.22 Fc fusion (100 nM) and AT8 (1:200 dilution, Invitrogen) in M ...
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bioRxiv - Molecular Biology 2021Quote: ... The fusion proteins were purified with HisPur Cobalt Resin (Thermo Scientific). For GST pull-down assay ...
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bioRxiv - Cell Biology 2020Quote: To localize GFP-tagged fusion proteins with BODIPY Red (Molecular Probes), transfected cells were grown overnight (16-20h ...
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bioRxiv - Cell Biology 2020Quote: ... To localize GFP-tagged fusion proteins with MitoTracker Red (Molecular Probes), after 16h transfection ...
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bioRxiv - Biophysics 2022Quote: ... adding PreScission protease (Thermo Scientific: cat 88946, 2unit/0.2mg fusion protein), and rocking gently overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were produced using Expi293F cells (Life Technologies). Spike proteins for enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was expressed using Expi293F™ Cells (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins or commercially available RNase T1 (Thermo Fisher Scientific) were mixed with 0.5 pmol substrate RNAs in 10 µl RNase reaction buffer with EDTA (20 mM Tris-HCl pH7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Concentrations of recombinant proteins were measured by NanoDrop OneC (ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were prepared by Expi293 Expression System (Thermo Fisher) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2020Quote: ... 40 μL of recombinant protein G agarose beads (Thermo Fisher) were washed three times with 1 mL of ChIP dilution buffer by resuspending beads in the buffer ...
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bioRxiv - Immunology 2021Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Physiology 2022Quote: Recombinant proteins were produced in Expi293F cells (Thermo Fisher Scientific) by transient transfection ...
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bioRxiv - Microbiology 2022Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µg (60 pmol) of recombinant Cas9 protein (A36498, Invitrogen) was incubated with 180 pmol of sgRNA in a 20 µL reaction for 20 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Recombinant proteins were produced in Expi293F cells (ThermoFisher, Cat. #A14635) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... Human embryonic kidney 293 cells containing SV40 large T-antigen (HEK293T) were cultured in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Human embryonic kidney 293T cells containing SV40 large T-antigen (HEK293T) were maintained in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Immunology 2023Quote: ... they were incubated for 10 min at room temperature with a combined Fc block solution (2μL Human Fc block [BD Biosciences, San Jose, CA, USA] + 3μL heat-inactivated human AB serum [Fisher Scientific, Waltham ...
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bioRxiv - Immunology 2021Quote: ... was coated with 2μg/mL goat anti-human Fc antibody (Thermo Fisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) and anti-human IgG Fc specific HRP conjugated secondary antibodies (ThermoFisher Scientific) were added ...
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bioRxiv - Immunology 2022Quote: ... medium supplemented with 10 ng/ml human GM-CSF and 20% FCS (Gibco). For the purposes of proliferation assays ...
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bioRxiv - Immunology 2023Quote: ... or HRP-conjugated rabbit anti-human IgG Fc antibodies (Invitrogen, 31423, 1:5,000) diluted in blocking buffer were added to the wells incubated with phages or recombinant proteins ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Plant Biology 2021Quote: ... to create a fusion to the Maltose-binding protein (MBP) or into pDEST15 for fusions to Glutathion-S-transferase (GST) (ThermoFisher). To create GUS reporter constructs ...
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bioRxiv - Microbiology 2022Quote: ... Fecal antigen concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Immulon IV ELISA (Nunc ...
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bioRxiv - Biochemistry 2021Quote: ... the plates were incubated for 1 hr with horseradish peroxidase-(HRP) conjugated anti-human IgG1 (Thermo Scientific, A10648). Detection was performed using a two-component peroxidase substrate kit (BD biosciences ...
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bioRxiv - Immunology 2023Quote: ... post-dose 3 and 6-months post-dose 3 using mouse anti-human IgG1 biotin (Thermo Fisher Scientific) and mouse anti-human IgG4 biotin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... ACE2-Fc was purified over Protein A Agarose (Thermo Fisher; 15918014). Once the Protein A Agarose was equilibrated in PBS buffer ...
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bioRxiv - Immunology 2023Quote: ... KIR-Fc were conjugated to protein-A Alexa 647 (Invitrogen, #P21462) overnight at 1:4 (molar ...
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bioRxiv - Cancer Biology 2022Quote: ... we delivered recombinant Streptococcus pyogenes Cas9 protein (TrueCut™ Cas9 Protein v2; Thermo Fisher Scientific) together with the crRNA and tracrRNA into in vitro fertilized eggs of C57BL/6J mice according to the institutional procedure of Kyoto university (Ishibashi et al. ...
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bioRxiv - Developmental Biology 2024Quote: Recombinant FGF10 protein was labeled using an Alexa Fluor 488 Microscale Protein Labeling Kit (Invitrogen). To reduce the loss of physiological activity of FGF10 ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...