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Citations for Thermo Fisher :
551 - 600 of 5300 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibodies were obtained from Molecular Probes.
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were diluted 1:500 (goat anti-Rabbit IgG Alexa-647 H+L Invitrogen #A21245 and goat anti-Mouse IgG Alexa-405 H+L Invitrogen #A31553), and were incubated for 2-3 hours at room temperature in PB containing 3% NGS and 0.3% TX ...
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bioRxiv - Cell Biology 2023Quote: ... slides were washed with PBS and incubated for 2 h at room temperature with the secondary antibody (goat anti-rabbit IgG H+L conjugated with Alexa Fluor 488, Thermo Scientific), washed with PBS and mounted in VECTASHIELD® HardSet™ antifade medium with DAPI (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:500 in PB 0.1 M containing 0.3% Triton X-100 and 3% NGS.
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bioRxiv - Neuroscience 2023Quote: ... we washed 5 times with PB 0.1 M and incubated them for 1 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:1000 in PB 0.1 M containing 1% NGS ...
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bioRxiv - Neuroscience 2024Quote: ... HEK cells below passage 10 are plated into a 15 cm dish/virus (Fisher 08-772-24) in DMEM-H (DMEM-h (invitrogen 10569010) + NEAA (Invitrogen 11140050) + 10% FBS (invitrogen 16140071 ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS + 3% NDS at RT for 1 h followed by Goat anti Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (DyLight 405, Invitrogen #PI35500) in PBS + 3% NDS at RT for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... DIV5 mouse hippocampal neurons were incubated with 20 nM Reelin or Mock medium for 2 h after 4 h of incubation in depletion medium (only neurobasal medium (Gibco, Thermo Fisher) plus 1% GlutaMAX) ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 568 goat anti-mouse IgG (H+L) and Alexa Fluor 488 goat anti-rat IgG (H+L) (both from Invitrogen Molecular Probes), for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-Mouse IgG (H+L) Alexa Fluor Plus 488 and goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 (1:200, Thermo Fisher). Finally ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were anti-rabbit H+L Alexa Fluor 568 and anti-mouse H+L Alexa Fluor 488 (1:500; Life Technologies). After IHC ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with RSL3 for 2 h or IKE for 6 h together with the indicated compounds before BODIPY 581/591 C11 (Thermo Fisher) was added in a final concentration of 2 μM per well and incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... growth medium (200 µL, 24 h), bacterial cultures (200 µL, 24 h of growth)) and pre-cleaned type 1 glass vials (VOA; Thermo Scientific) were chilled on ice for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies used for immunofluorescence studies were Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) (#A11012) or Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) (#A11001) from Life Technologies Corporation ...
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bioRxiv - Physiology 2024Quote: ... then incubated at RT for 2 h with goat anti-Rabbit IgG (H+L) Alexa Fluor Plus 488 (ThermoFisher, cat#A32731) and goat anti-guinea pig IgG (H+L ...
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bioRxiv - Plant Biology 2024Quote: ... Yeast cells were incubated with Goat Anti-Mouse IgG (H+L) Alexa Flour Plus 488 or Goat Anti-Rabbit IgG (H+L) Alexa Flour Plus 647 (Invitrogen, USA) at a ratio of 1:500 for 4hr at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... membranes were incubated with respective horseradish peroxidase-conjugated secondary antibodies for 1 h: anti-mouse (1:4000, 1 h, Thermo Fisher Scientific Cat# G-21040 ...
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bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 488 goat anti-mouse IgG (H + L) (#A11029) and Alexa Fluor 568 goat anti-rabbit IgG (H + L) (#A11036) were purchased from Thermo Fisher.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed three times with 1X PBT for 5 minutes each and then incubated for 1 h at room temperature with secondary antibody (goat anti-rabbit IgG (H+L) Alexa Flour 546 (Invitrogen, A11035) or goat anti-mouse IgG (H + L ...
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bioRxiv - Plant Biology 2020Quote: ... reinhardtii strains WT-SAG and CRISPRXylT1A_1 were grown axenically in tris-acetate-phosphate (TAP) medium (Thermo Fisher Scientific) in a Memmert IPP 100Plus incubator on a 12h day / 12h night cycle ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Flowthrough was then collected after spinning the suspension down in a cellulose acetate spin column (Thermo Scientific #69702), separating the His-tagged UBA1 from the other components ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The reaction was stopped by ethanol precipitation: 1 μl 3 M RNAse-free sodium acetate (pH 5.5) (Ambion), 1 μl RNAse-free glycogen (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Corning™ Accutase™ detachment solution and phorbol 12-myristate 13-acetate (PMA) were obtained from Fisher Scientific (cat ...
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bioRxiv - Molecular Biology 2022Quote: ... run at 150 V for 90 minutes in 1× NuPAGE Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific). Proteins were electrotransferred onto an Immobilon-fl polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were loaded onto a NuPAGE® Novex® 3–8% Tris-Acetate Gel3–8% (Thermo Fisher Scientific) and run in Novex Tris-Acetate SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed using NuPAGE 4-12% Bis-Tris and 3-8% Tris-Acetate gels (Life Technologies). Nitrocellulose membranes were developed with horseradish peroxidase (HRP ...
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bioRxiv - Genetics 2020Quote: ... reduced whole cell lysates were electrophoresed on a NuPAGE 7% Tris-Acetate pre-cast 1.0 mm gels (Invitrogen) for 50 minutes at 150 V and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: ... 200 µg of human serum) were separated by SDS-PAGE on 3-8% tris-acetate gels (NuPAGE, Invitrogen) under reducing conditions at 150 V (cellular fraction ...
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bioRxiv - Immunology 2020Quote: ... samples were loaded under non-reduced conditions on a precasted 3-8% Tris-Acetate SDS-PAGE gel (Invitrogen). Proteins were transferred to a nitrocellulose membrane using an iBlot2 dry blotting system (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: Up to 10μg of total protein was resolved on NuPAGE™ 3-8% Tris-Acetate Protein Gels (Invitrogen) in 1x NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... The mobile phase consisted of buffer A: 5 mM ammonium acetate in water (LC/MS grade, Thermo Scientific) and buffer B ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded onto a NuPAGE® Novex® 3–8% Tris-Acetate (Thermo Fisher, Waltham MA, USA) and run at 100V during 5h ...
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bioRxiv - Microbiology 2023Quote: ... Acetate and lactate were measured using an ICS-1100 series ion chromatograph (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a polysulfonate ion-exclusion column (Metrosep A Supp 5) ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was precipitated with 2.5x volumes ice-cold ethanol and 0.1x volume 3M sodium acetate pH 5.5 (ThermoFisher #AM9740) overnight at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... and then analysed on Novex WedgeWell 4-12 % Tris-Glycine Gels or 3-8 % Tris-Acetate gels (Invitrogen). Tris-Glycine gels were run at 180 V for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The mobile phase consisted of buffer A: 5 mM ammonium acetate in water (LC-MS grade, Thermo Scientific) and buffer B ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was purified via ethanol precipitation (final concentrations of 0.3 M sodium acetate pH 5.2, 0.3 µg/mL glycoblue (Invitrogen #AM9516), and 70% ethanol ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 were differentiated by the addition of 150ng/mL phorbol 12-myristate 13-acetate (PMA) (ThermoFisher Scientific). The epithelial cell lines were cultured according to ATCC guidance for the specific cell line and as adherent cells ...
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bioRxiv - Cell Biology 2024Quote: ... Five μL of each sample were loaded per lane on a 4-12% Tris-acetate gel (Invitrogen XP04125BOX), and proteins were transferred to a 0.2 μm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Corning™ Accutase™ detachment solution and phorbol 12-myristate 13-acetate (PMA) were obtained from Fisher Scientific (cat ...
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bioRxiv - Biochemistry 2024Quote: ... total RNA extraction was carried out using acetate-saturated phenol/CHCl3 at pH 4.5 (Thermo Fisher Scientific, AM9720). The isolated RNA was then resuspended in 10 mM NaOAc/HOAc at pH 4.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... protein samples were resolved by SDS-PAGE on pre-cast NuPAGE™ gels (1.0 mm 4–12% Bis-Tris or 1.5 mm 3–8% Tris-Acetate for HMW TRIM37, Invitrogen) with molecular weight ladders (PageRuler Plus or HiMark pre-stained protein standard for HMW TRIM37 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were buffer exchanged into 500 mM ammonium acetate using Zeba Spin 7K MWCO desalting columns (ThermoFisher Scientific). Samples were analysed by nanoelectrospray ionisation MS using a quadrupole-orbitrap MS (Q-Exactive UHMR ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... Undeveloped photoresist was washed off with SU-8 developer (1-methoxy-2-propanol acetate, Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... The mobile phase consisted of buffer A: 5 mM ammonium acetate in water (LC/MS grade, Thermo Scientific) and buffer B ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with PBS and incubated with corresponding secondary antibodies goat anti-rabbit IgG Alexa Fluor 555 (H+L) and goat anti-mouse IgG (H+L) Alexa Fluor 647(all Thermo Fisher Scientific) for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... stained for 1.5 h with primary and for 1 h with species-matched secondary antibodies at RT and mounted on microscopy slides (Thermo Fisher Scientific) in Fluoromount-G (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa Fluor 488-conjugated goat anti-rat IgG (H + L) antibodies and Alexa Fluor 594-conjugated goat anti-mouse IgG (H + L) antibodies (ThermoFisher Scientific, A11005) were used as secondary antibodies ...