Labshake search
Citations for Thermo Fisher :
451 - 500 of 5300 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Lysates were separated using discontinuous SDS-PAGE to resolve proteins <200kDa or Tris-Acetate gels (NuPAGE, Invitrogen) to resolve proteins >200kDa ...
-
bioRxiv - Microbiology 2020Quote: ... 1/10 volume of 3 M Sodium Acetate (pH 5.2) and 1 μl of GlycoBlue (Life Technologies). The precipitate was pelleted by centrifugation (30 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... Equal protein lysates were run on Novex 3–8% Tris-acetate 15 Well Mini Gels (EA03785BOX, Invitrogen) and transferred to Immobilon-P membranes (IPVH00010 ...
-
bioRxiv - Cell Biology 2022Quote: ... 30µg/well of each sample was loaded onto NuPAGE Novex 3-8% Tris-Acetate gel (Thermo Fisher), and proteins were separated at a constant voltage of 150V for 1.5 hours ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated using NuPAGE 3–8% Tris-Acetate Protein Gels (Life Technologies/Gibco®, Karlsruhe, Germany). For the detection of fibronectin ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated using NuPAGE 3–8% Tris-Acetate Protein Gels (Life Technologies/Gibco®, Karlsruhe, Germany). For the detection of fibronectin ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by NuPAGE 3-8% or 4-12 % Tris-Acetate Midi Gel (Invitrogen, Cat# WG1402BX10) and transferred to nitrocellulose membranes (Fisher ...
-
bioRxiv - Cell Biology 2024Quote: ... 50 μg/lane of lysates were electrophoresed on NuPAGE 3–8% Tris-Acetate Gels (Thermo Fisher Scientific), while 10 μg/lane of lysates were electrophoresed on Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... A standardized quantity of protein was resolved using NuPAGE Tris-Acetate Protein Gel (3-8%)(Thermo Fisher) or NuPAGE Bis-Tris Protein Gel (4-12% ...
-
bioRxiv - Cell Biology 2023Quote: Equal amounts of reduced protein were loaded into 3-8% Tris-Acetate Mini Gels (Thermo Fisher Scientific) and separated electrophoretically by molecular weight ...
-
bioRxiv - Bioengineering 2024Quote: ... The denatured protein samples were separated on NuPAGE™ 3–8% Tris-acetate gels (Cat #EA03785BOX, Invitrogen) according to manufacturer’s instructions and subsequently stained with Pierce™ silver stain kit (Cat # 24612 ...
-
bioRxiv - Immunology 2024Quote: ... ≈2.5x105 cells were run in each lane of a 7% NuPAGE tris-acetate gel (Invitrogen, Carlsbad, CA) and transferred to Immobilon-FL polyvinylidene difluoride membrane (EMD Millipore ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 10μL soluble protein was loaded into a pre-cast NuPAGE 3-8% Tris-Acetate gel (Invitrogen). Proteins were transferred using an iBlot2 (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... ethanol precipitation with ammonium acetate was performed according to the method presented in MEGAclear kit instruction (Invitrogen). To this end ...
-
bioRxiv - Molecular Biology 2020Quote: ... Expression of DOT1B-Myc-BirA* was induced by addition of 5 ng/ml tetracycline for 24 h and cells were further incubated for 24 h with 50 µM D-biotin (Invitrogen) and 5 ng/ml tetracycline ...
-
bioRxiv - Genomics 2020Quote: ... Goat anti-Rabbit IgG (H+L)-HRP (Bio Rad, 1706515) and Goat anti-Mouse IgG (H+L)-HRP (Invitrogen, 31430) were used as secondary antibody for western blotting.
-
bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit Alexa Fluor 488 IgG H+L (1:400, A11008) and goat anti-mouse Alexa Fluor 568 IgG H+L (1:250, A11004) (Invitrogen, ThermoFisher).
-
bioRxiv - Microbiology 2020Quote: ... Alexafluor 488 F(ab’)2 fragment of goat anti-rabbit IgG (H+L) (1:100) and Alexafluor 488 goat anti-mouse IgG (H+L) (1:100) were all purchased from ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2 h at room temperature with goat anti-rabbit (H+L) or goat anti-mouse HRP-linked secondary antibodies (Invitrogen) respectively ...
-
bioRxiv - Cancer Biology 2020Quote: ... Two siRNA oligonucleotides to cavin3 or BRCA1 were found to reduce protein levels (oligo 1 and oligo 2) and were transfected into cells at 24 h and 48 h after plating using Lipofectamine 2000 reagent (Invitrogen) with a ratio of 6 μl Lipofectamine to 150 pmol siRNA ...
-
bioRxiv - Cancer Biology 2020Quote: ... Then the cells were washed with PBS and incubated for 1 hour with secondary antibody [Alexa Fluor-488 goat anti-mouse immunoglobulin G (IgG) (H+L) conjugate or anti-rabbit IgG (H+L) conjugate (Invitrogen)] ...
-
bioRxiv - Cell Biology 2020Quote: ... Hematoxylin and Eosin (H&E) tissue slides were stained according to manufacturer’s instructions with Hematoxylin and Eosin (H&E) (Thermo Fisher).
-
bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 594- or Alexa Fluor 647-conjugated highly cross-absorbed goat anti-mouse IgG (H+L) or goat anti-rabbit IgG (H+L) (Invitrogen), HRP-conjugated goat anti-rabbit or goat anti-mouse IgG (Jackson ImmunoResearch).
-
bioRxiv - Immunology 2020Quote: ... Goat anti-Mouse IgG (H+ L) Alexa Fluor 555 and Goat anti-Mouse/Rabbit IgG (H+L) FITC was purchased from Invitrogen; Mouse anti-Flag/HA-tags beads was purchased from Abmart ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2 h at room temperature with goat anti-rabbit (H+L) HRP-linked secondary antibody (Invitrogen) respectively ...
-
bioRxiv - Molecular Biology 2022Quote: ... Due to long incubation time from seeding to fixation (72 h post-transfection, 96 h post-seeding) cells were singularized by trypsinization (Gibco) 48 h post-transfection to increase spreading of the cells ...
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were subsequently incubated in blocking buffer containing horseradish peroxidase (HRP)-conjugated anti-mouse IgG (H+L) (1:5,000, Fisher 62-6520) or HRP-conjugated anti-rabbit IgG (H+L) (1:5,000, Life Technologies 31462) for 1 h ...
-
bioRxiv - Microbiology 2024Quote: ... The secondary antibodies were applied O/N on a shaker: Alexa Fluor 594 anti-mouse IgG (H+L) and Alexa Fluor 488 anti-rabbit IgG (H+L) (both Invitrogen), both at 1:500 dilutions in 2% BSA/PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells expressing H/F-BAZ2AWT or H/F-BAZ2AΔTAM were collected and their total RNA purified with TRIzol reagent (Life Technologies) as described above ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were fed in full media for 1 h prior to 8 h starvation in Earle’s Balanced Salt Solution (EBSS; Life Technologies).
-
bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated with HRP-conjugated goat anti-rabbit IgG (H+L) or HRP-conjugated goat anti-mouse IgG (H+L) secondary antibody (Invitrogen). Finally ...
-
bioRxiv - Genomics 2023Quote: ... remaining RNA was digested with RNase mix (0.25U/μL RNase H, 2.5% Invitrogen RNase cocktail mix, 1x RNase H buffer) at 37 °C for 1 hour followed by two PBST washes ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were all pulsed for 1 h and chased for 1 h with either with 100 µg/mL Alexa488-conjugated dextran (ThermoFisher) or 50 µg/mL Alexa546-dextran (ThermoFisher) ...
-
bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
-
bioRxiv - Plant Biology 2024Quote: ... Excised leaves from six 6-week-old wild type and mutant plants were exposed to 1 h 40 °C (control: 1 h 25°C) and total RNA was extracted using TriZol (Invitrogen) followed by DirectZol (Zymo Research ...
-
bioRxiv - Molecular Biology 2024Quote: ... secondary antibody solution for 1 hour (Goat anti-Mouse IgG H+L 568, Invitrogen #A-11031; Goat anti-Rabbit IgG H+L 488, Invitrogen #A-11034 ...
-
bioRxiv - Plant Biology 2024Quote: ... About 5 µg were either treated or not with Ribonuclease H (RNase H 5 U/μL) (Thermo Fisher Scientific®), and incubated with 2 µL S9.6 antibody (Merck ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA extraction from cells was done at either 6 h or 24 h after AMO transfection using Trizol (Invitrogen) followed by either poly(A ...
-
bioRxiv - Immunology 2024Quote: MC38 cell lines (derived from C57BL/6, H-2kb/H-2Db, kindly gifted by Dr. Ramon Arens) were cultured in IMDM (Gibco) supplemented with 10% FCS (Serana ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the membrane was incubated for 1 h at room temperature with HRP conjugated anti-mouse IgG (H+L) secondary antibodies (Cat#31430, ThermoFisher), and washed again with PBST for 3 times (5 min each) ...
-
bioRxiv - Neuroscience 2020Quote: ... for 4 h and DAPI (Thermo Fisher Scientific) staining was used to visualize cytoarchitecture (1:5000 ...
-
bioRxiv - Genetics 2021Quote: ... and Maxima H minus reverse transcriptase (Thermo Fisher) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was treated with RNAse H (ThermoFisher Scientific) and the samples were used to perform qPCRs using LightCycler™ 480 SYBR Green I Master mix (Roche) ...
-
bioRxiv - Neuroscience 2021Quote: ... and loaded with 5 µM coelenterazine h (Invitrogen) for 4 hours at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4µl ReverTAid H Minus RT (Thermo Fisher Scientific) were added ...
-
bioRxiv - Cancer Biology 2020Quote: ... or Maxima H Minus Reverse Transcriptase (Thermo Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... goat anti-rabbit IgG (H+L) (A27036, Invitrogen) or goat ant-rat IgG (H+L ...
-
bioRxiv - Bioengineering 2021Quote: ... and 2 μl Maxima H- RTase (EP0751, Thermofisher). Reverse transcription was performed at 42°C overnight.
-
bioRxiv - Systems Biology 2021Quote: ... 12 U/µl Maxima -H reverse transcriptase (ThermoFisher) and 6,000 U/µl NxGen RNase Inhibitor (Lucigen) ...
-
bioRxiv - Microbiology 2020Quote: ... RT buffer (Maxima H Minus Reverse Transcriptase, ThermoFisher), RNase OUT (Life Technologies ...