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Citations for Thermo Fisher :
801 - 850 of 5300 citations for Octreotide acetate impurity H since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of ion-pairing agent (0.1% o-toluidine blue, Carolina Biological) and 3 µL of ethyl acetate (ACS grade, Fisher Scientific). The mixture was vortexed and centrifuged briefly ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 95°C for 5 mins and spun at 21 300 rcf for 5 mins prior to loading in NuPAGE Tris-Acetate gels (Invitrogen). Gels were run at 150 V for 1 h and transferred onto 0.2 µm PVDF membranes using the High MW setting on the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... 5µl samples of the PCR2 products were fractionated on a 2% agarose Tris-Acetate-EDTA (TAE) gel using Sybr Safe dye (Invitrogen) alongside a set amount of 100bp DNA ladder (NEB ...
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bioRxiv - Microbiology 2023Quote: ... were stained with uranyl acetate and Reynolds lead citrate and examined at 100 kV with a PHILIPS EM208S TEM (FEI - ThermoFisher) equipped with the Megaview III SIS camera (Olympus).
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bioRxiv - Microbiology 2024Quote: ... Protein extract was heat-denatured for 10 min at 80°C in NuPAGE LDS Sample Buffer an an equivalent of 2 µg total protein was separated by SDS-PAGE on a NuPAGE 4-12% Tris-Acetate Midi Gel in MOPS buffer (Invitrogen). Protein was transferred to a 0.45 µm PVDF Membrane (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... Purified proteins were stored at −80°C or refolded by dialysis against refolding buffer (20mM sodium acetate and 0.005% NaN3 (pH 4.5)) using SnakeSkin™ Dialysis Tubing (MW cut-off: 3500 Da, Thermo Scientific). Purified proteins were analyzed by SDS-PAGE under reducing conditions.
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bioRxiv - Biochemistry 2023Quote: The protein preparation was stored at −20°C before being buffer exchanged into 200 mM ammonium acetate (pH 6.8) by multiple rounds of concentration and dilution using the PierceTM protein concentrators (Thermo Fisher). The sample was then diluted to 2 µM monomer concentration immediately before the measurements ...
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bioRxiv - Biochemistry 2023Quote: ... buffer was exchanged to 200 mM ammonium acetate (pH 4.5) with a Zeba™ 7 K MWCO desalting column (ThermoFisher). After buffer exchange ...
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bioRxiv - Biochemistry 2023Quote: ... Concentrated virus was applied through a 0.45 μm surfactant free cellulose acetate syringe filter (Nalgene, Fisher Scientific #09-740-106) and immediately used or stored at -80ºC for up to one month ...
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bioRxiv - Cell Biology 2023Quote: ... NeutrAvidin bead acetylation was necessary to stop the Neutravidin being cleaved from the agarose beads during on-bead digestion and performed prior to the day of pulldown by two 30 min incubations of beads with 10 mM Sulfo-NHS acetate (ThermoFisher) on a rotating wheel followed by quenching in 90 mM Tris-HCl pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... carrying plasmids expressing DB-fused NS5 sequences were co-transformed using a standard lithium/acetate procedure with 10 ng of linearized pPC86 empty vector (Invitrogen) and 3 µL of PCR products generated from cDNA encoding TYK2 ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE western blot was performed by loading 20 μg of each S-fraction and equal volume of the P-fraction onto precast Tris-Acetate SDS-PAGE (Novex, Invitrogen). Western blots were performed as before ...
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bioRxiv - Cell Biology 2024Quote: ... Resuspend in 50-500 µL of 1.11x tagmentation buffer (66 mM Tris-acetate (pH=7.8, BP-152, Thermo Fisher Scientific), 132 mM K-acetate (P5708 ...
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bioRxiv - Neuroscience 2022Quote: ... SDS-PAGE western blot was performed by loading 20 μg of each S-fraction and double the volume of the P-fraction onto pre-cast Tris-Acetate SDS-PAGE (Novex, Invitrogen). Western blots were performed as before ...
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bioRxiv - Molecular Biology 2022Quote: ... quenched in 100 mM Tris-HCl pH 8.0 and analyzed on a NuPAGE™ 3–8 % Tris-Acetate gel (Invitrogen). Fractions containing protein bands corresponding to the expected size of a Pol II–APT complex were dialyzed against 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 µl water and precipitated in 0.75 M ammonium-acetate and 10 µg glycogen (#R0551 Thermo Scientific) by adding 125 µl ethanol (100%) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were size-fractionated on precast 10 cm 4-12% Bis-Tris or Tris-acetate gels (Thermo Fisher Catalog #NP0336BOX), then electroblotted onto nitrocellulose membranes (Thermo Fisher Catalog #88025) ...
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bioRxiv - Cell Biology 2022Quote: ... All samples were boiled for 5 min prior to separation by SDS PAGE on precast 3-8% Tris-Acetate NuPAGE gels (Invitrogen). SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Biochemistry 2023Quote: ... at 70 °C for 7 min then precipitated in 75% ethanol with 10% volume of sodium acetate (3 M, pH 5.5) and GlycoBlue (1 μL, Invitrogen AM9515). The RNA fragments were dissolved in nuclease-free H2O at a concentration of 100 ng/μL ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylated mRNA fragments were precipitated in 100% ethanol with 40 μl 3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion) at -20°C for >16 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was precipitated by transferring 320 μl of supernatant to a new RNase-free microcentrifuge tube and adding 32 μl of 3 M sodium acetate (pH 5.5) (Invitrogen AM9740), 2.5 μl of RNA-grade glycogen (Thermo Scientific R0551) ...
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bioRxiv - Cell Biology 2024Quote: ... 30 to 50 μg of protein extracts were resolved by SDS-polyacrylamide gel electrophoresis using 4–12% NuPAGE Bis-Tris gradient gel or 3–8% Tris-acetate gel and run (Invitrogen) and MOPS or Tris-acetate running buffer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... a solution of 1 × 106 cells/ml supplemented with 5 ng/ml phorbol 12-myristate 13-acetate (PMA, ThermoFisher Scientific) was seeded in each well of a 12-well cell culture plate (TPP ...
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bioRxiv - Biochemistry 2024Quote: ... Excess solution was dabbed away and the grids were negative stained with 10 uL of 5% uranyl acetate (ThermoFisher Scientific) for 5 minutes before excess solution was dabbed away ...
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bioRxiv - Genomics 2024Quote: ... was prepared fresh each time by measuring NaIO4 powder and resuspending to a concentration of 4 mg/ml in aqueous solution of 200 mM sodium acetate (pH 5.5) (Invitrogen, AM9740). Input RNA (up to 5 μg ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were run on a Criterion XT 3-8% Tris-Acetate PAGE gel in 1x XT Tricine and analysed by silver staining (SilverQuest, Invitrogen) or immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... The aqueous layer was transferred into a new tube and the RNA was precipitated by adding 3 M sodium acetate pH 5.2 (Thermo Scientific), glycoblue (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... Proteins were loaded and run on a 4-12% Bis-Tris pre-cast gel or a 3-8% Tris-Acetate pre-cast gel (Invitrogen) and were electrophoretically transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... 96 h and 1 week following manufacture protocol (Invitrogen #C10337). Percentage of B16-F1-HA and B6-F1-HAE1 cells in S phase was calculated ...
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bioRxiv - Cell Biology 2020Quote: ... 16 h before harvesting the cells Colcemid (Gibco, #15212-012) was added at the concentration of 0.08 μg/ml without replacing the culture medium ...
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bioRxiv - Cell Biology 2020Quote: ... donkey anti-rabbit IgG (H+L) Alexa-594 (Thermofisher A21207) (dilution 1:500) ...
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bioRxiv - Genomics 2020Quote: ... Add the solutions and RNase H (Thermo Fisher cat. 18021071) listed in Table III ...
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bioRxiv - Genomics 2020Quote: 11 μL of Maxima H Minus Reverse Transcriptase (Thermo Fisher),
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor568 donkey anti-mouse IgG (H+L) (Thermo Fisher Scientific Cat#A10037 ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at 37 °C in opti-MEM (Gibco). Cells were washed 3x after transfection with media ...
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bioRxiv - Molecular Biology 2020Quote: ... or Goat anti-rabbit IgG (H+L) HRP (ThermoFisher Scientific). Blots were developed using Super Signal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Invitrogen, A27036). Immunodetection was performed using Western Blotting Luminol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Invitrogen, A28177); Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.09 μL Ambion RNase H (E. coli) (Invitrogen, AM2293). Second strand synthesis was performed by incubating samples at 16°C for 150 min ...
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bioRxiv - Neuroscience 2020Quote: ... CHO cells were harvested and loaded with Coelenterazine H (Invitrogen) for 4 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... followed by second strand cDNA synthesis with RNase H (Invitrogen) and DNA Pol I (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... HSS extracts were treated with RNase H (Thermo Fisher Scientific) or heat-inactivated RNase H (65°C for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... treatment in the presence of RNase H inhibitor (#AM2682, Ambion). Standardized quantities of RNA were reverse transcribed to cDNA using the SuperScript™ II First-strand Reverse Transcriptase system (#18064014 ...
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bioRxiv - Physiology 2020Quote: ... cDNA was then treated with RNase H (Thermo Fisher Scientific) according to the manufacturer protocol ...
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bioRxiv - Genomics 2021Quote: ... consisting of 30 units Maxima H- enzyme (Thermo Fisher, EP0753), 1x Maxima H- Buffer (Thermo Fisher) ...