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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed as previously described [39] using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Immunology 2021Quote: ... dilutions of IgG were co-incubated with the virus (1000-2000 TCID50) for 1 h at 37°C prior to addition of VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... Probes were mixed with Human Cot-1 DNA (Invitrogen, 15279011) and salmon sperm (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... and 5 µg of human Cot-1 DNA (ThermoFisher Scientific) and then denatured in deionized formamide (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% Primocin (Invivogen #ant-pm-1 ...
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bioRxiv - Immunology 2022Quote: ... anti-Human CD279(PD-1) antibody (Invitrogen, eBioscience, PE-Cyanine7), anti-Human CD223(LAG-3 ...
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bioRxiv - Immunology 2021Quote: ... 1:100 mouse anti-human CXCR5 PE-Cy7 (MU5UBEE, ThermoFisher) was added to each well at the start of stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human PHF-tauThr231 (1:1000; AT180, Thermo Scientific, USA). The secondary antibodies used against above were anti-Mouse Cy3 (1:200) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human PHF-tauThr231 (AT180; 1:1000, Thermo Scientific, USA), PHF-tauThr181 (AT270 ...
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bioRxiv - Microbiology 2022Quote: ... 0.3 μg of recombinant human CDKL5 (1-498) (ThermoFisher, A33353) was combined with 3 μg of recombinant human SQSTM1/p62 protein (GeneTex ...
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bioRxiv - Immunology 2019Quote: ... ProcartaPlex Human Cytokine/Chemokine/Growth Factor Panel 1 (Thermo Scientific), as previously described [14] ...
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bioRxiv - Bioengineering 2019Quote: ... then with human-CD68 (FITC conjugated 1:20 dilution; Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-human GRZMK (Invitrogen, LS-C119554-50, 1:100), rabbit anti-human E-cadherin (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% antibiotic-antimycotic (ThermoFisher #1540062) ...
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bioRxiv - Microbiology 2022Quote: ... 1:5000 dilution rabbit anti-human Lcn2 (Invitrogen, Cat. 702248), 1:1000 dilution rabbit anti-human peptidylarginine deiminases 4 (PAD4 ...
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bioRxiv - Neuroscience 2022Quote: ... Rat anti-human CD44 (1:500, ThermoFisher, 14-0441-82), Rabbit anti-human GFAP (1:400 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... goat anti-human Alexa Fluor 546 (1:400, Invitrogen Corporation) and goat anti-mouse Alexa Fluor 555 (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... and rat anti-human CD44 (1:50, clone IM7, Invitrogen). The secondary antibodies included alexa fluor 488 labelled donkey anti-mouse (xxx ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Gibco Human Episomal iPSC clone (Control 1, Thermo Fisher Scientific) and CW30318 (Control 2 ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... followed by anti-human IgG–Alexa594 (1:1000, ThermoFisher Scientific) for 30 min and analyzed by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... we re-suspended cells in PBS/ 0.2% human serum containing 2 µg/mL 7-aminoactinomycin D (7-AAD) (Invitrogen, Cat# A1310). We carried out isotopic controls with irrelevant mouse IgG1-APC ...
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bioRxiv - Microbiology 2020Quote: ... vif vpr and vpx coding sequences were amplified with specific primers from pKP59-HIV-2 ROD plasmid and cloned into pcDNA3.1 vector (Invitrogen). Expression vectors encoding the six different mutants of HIV-2 Env cytoplasmic tail were created by introducing stop codon in different position within the cytoplasmic tail by site-directed mutagenesis using the QuickChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Cancer Biology 2022Quote: ... Dilute 2.5μg of lentiviral expression plasmid and 2.5 μg of Lenti-Pac HIV mix into 200μl of Opti-MEM® I (Invitrogen). In a separate tube diluting 15 μl of EndoFectin Lenti into 200μl of Opti-MEM I ...
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bioRxiv - Neuroscience 2023Quote: ... and 1.3 nM human insulin-like growth factor 1 (hIGF-1, Thermo Fisher Scientific). The growth media were changed after 24 h and further regularly changed every 4 days ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL pre-washed magnetic-conjugated protein G beads (Dynabeads protein G, Invitrogen) were added to the reactions followed by the incubation for 1 hour at 4°C in the thermomixer (750 rpm) ...
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bioRxiv - Genomics 2021Quote: ... Antibodies were conjugated to 30 uL of Protein G Magnetic Beads (Dynabeads, Thermo Fisher Scientific 10-003-D), per ChIP ...
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bioRxiv - Genomics 2021Quote: ... 40 µg of Oct4 antibody (R&D, AF1759) was coupled to Dynabeads protein G (Thermo Fisher Scientific, 10009D) for 2 hours at room temperature for each sample ...
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bioRxiv - Biochemistry 2024Quote: ... and 200 µg of lysate was immunoprecipitated with 40 µL Protein A dynabeads (Fisher Scientific, 10-001-D) pre-coupled to 8 µg anti-CD44 (Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
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bioRxiv - Cell Biology 2021Quote: ... The virus packaging was performed in HEK293FT cells (Thermo Fisher Scientific) based on a calcium precipitation method using pUMVC and pCMV-VSV-G vectors (37 ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Genetics 2020Quote: ... Cells were then transduced using CytoTune-iPS 2.0 Sendai virus (Invitrogen) as outlined in the supplier’s protocol at an MOI of 5:5:3 (KOS [Klf4 ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: Peak lists were searched against the complete human proteome (UniProt release 2014_08, with 68.049 proteins) using SEQUEST (Proteome Discoverer 1.3, ThermoFisher) as search engine ...
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bioRxiv - Immunology 2022Quote: ... Two micrograms of liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control (hepatocytes ...
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bioRxiv - Immunology 2021Quote: ... The isolated RNA was then evaluated in RT-qPCR using the TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific) on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... and infected at a MOI of 1 with the VSV-ΔG-Fluc-2A-GFP/VSV-G virus diluted in 10mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...