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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-human Cx36 (Invitrogen, 36-4600, 1:500) and chicken anti-GFP (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human thioredoxin 1 (hTrx) was purchased from ThermoFisher. The plasmid pET-TRSter for heterologous expression of human thioredoxin reductase (hTrxR ...
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Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxiv - Bioengineering 2023Quote: ... 1 ng/mL human FGF2 (ThermoFisher #68-8785-63), 1% antibiotic-antimycotic (ThermoFisher #1540062) ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-human AlexaFluor 647 (Invitrogen, A21445, 1:1000). For Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg/mL recombinant human insulin (Thermo Fisher Scientific), 0.55 µg/mL human transferrin (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... mice anti-human CXCL12 (1:50, clone 79018, Invitrogen), and rat anti-human CD44 (1:50 ...
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bioRxiv - Microbiology 2023Quote: ... or human TIA-1 (Assay ID 139893-Thermofisher scientific) or GFP targeting siRNA as a negative control (cat no ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and goat anti-human AlexaFluor488 (1:500, Invitrogen A11013) secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... anti-human PHF-tau (1:1000; Thermo Scientific, #MN1020), mouse anti-VGAT (1:500 ...
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bioRxiv - Immunology 2020Quote: ... 40 uL of a 1:1 mixture of protein A and protein G magnetic Dynabeads (Invitrogen) was added to each well and rocked at 4°C for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Immunology 2021Quote: ... RNA was reverse transcribed and amplified using TaqMan Fast Virus 1-Step Master Mix qRT-PCR Master Mix (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Virus titres were measured from a thawed aliquot by: (1) mixing 20 μl with 200 μl of TRIzol (Invitrogen); (2 ...
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bioRxiv - Microbiology 2019Quote: ... Two microliters of extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol and primers specific for HCoV-229E [30] or RSV-B [31] ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed with Taq-Man™ Fast virus 1-step master mix (ThermoFisher Scientific, Cat. 4444436) and primer-probe sets targeting SARS-CoV-2 nucleocapsid (N1 set ...
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bioRxiv - Microbiology 2020Quote: ... Two microliters of extracted RNA were amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2020Quote: ... one for virus quantitation (TCID50) and the other for vRNA extraction in TRIzol (ThermoFisher, 15596026; 1 in 3 dilution). Samples were stored at -80°C until required ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed according to the manufacturer’s instructions using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Pathology 2022Quote: ... RNA was reverse transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was amplified by RT-qPCR using the Taqman Fast Virus 1-step master mix (Thermo Fisher Scientific). Primers and probes targeting SARS-CoV-2 RNA-dependent RNA polymerase were described in [25] ...
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bioRxiv - Cell Biology 2019Quote: ... complementary DNA was then reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Invitrogen), using random hexamer oligonucleotides for priming ...
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bioRxiv - Cancer Biology 2019Quote: ... Expression levels of MYC and MEIS1 transcript were quantified using TaqMan Fast Virus 1-Step Master Mix (Life Technologies) using PrimeTime qPCR Probe Assays from Integrated DNA Technologies in a 3:1 ratio of primer to 5′ 6-FAM and 3′ TAMRA labeled probe ...
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bioRxiv - Microbiology 2020Quote: ... and infected at an MOI of 1 with the VSVΔG:mNeon/VSV-G virus diluted in 10 mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Immunology 2022Quote: ... according to manufacturer’s recommendation and qRT-PCR was performed using TaqMan Fast Virus 1-Step Mix (Applied Biosystems, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2022Quote: ... and then quantified by qRT-PCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with the TaqMan™ 2019nCoV Assay Kit v1 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The RT-PCR was performed using the Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a QuantStudio3 (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
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bioRxiv - Biochemistry 2019Quote: U-2 OS cells (human bone osteosarcoma epithelial cells, ATCC Number: HTB-96 [17]) were grown in D-MEM Glutamax (Gibco) supplemented with final concentrations of 10% fetal calf serum (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
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bioRxiv - Neuroscience 2020Quote: The human cells SH-SY5Y were grown in D-MEM (Dulbecco’s Modified Eagle medium; Gibco®, Life technologies Paisley, UK) supplemented with 10% fetal bovine serum and 1% of penicillin/streptomycin solution (100 U/mL) ...
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bioRxiv - Cell Biology 2021Quote: U-2 OS cells (human bone osteosarcoma epithelial cells, ATCC Number: HTB-96) (88) were grown in D-MEM Glutamax (Gibco) supplemented with final concentrations of 10% fetal calf serum (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... The pENTR-hMCAK construct was generated by amplifying the coding sequence of human MCAK by PCR and inserting it into a pENTR entry clone using the pENTRTM/D-TOPO kit (ThermoFisher). The pFB-6HF2hMCAK construct was created from pFB-6HFRET2-DEST vector and pENTR-hMCAK constructs using Gateway® cloning technology (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: Human foreskin fibroblasts (HFFs) monolayers were grown in DMEM GlutaMAXTM (4.5 g/L D-glucose) (Life Technologies #10-566-024) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... The process entailed cloning the various human ACTL7B gene variants into the pENTR TOPO vectors using the pENTR d-Topo cloning kit (Invitrogen), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... fibroblast-derived virus stocks were treated with TurboDNase (Thermo Fisher). For this ...
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bioRxiv - Immunology 2021Quote: ... Pelleted virus was resuspended in Opti-MEM (Gibco 31985-070) and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... In brief the virus was diluted in DMEM (ThermoFisher Scientific) to achieve a multiplicity of infection (MOI ...
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bioRxiv - Pathology 2022Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix were used ...
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bioRxiv - Molecular Biology 2020Quote: ... and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) with 250 μM deoxy nucleoside triphosphate (dNTP ...
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bioRxiv - Microbiology 2019Quote: ... and Moloney murine leukemia virus reverse-transcriptase (MMLV-RT, Invitrogen). Subsequent PCR amplification performed in a thermocycler using specific primers ...
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bioRxiv - Genetics 2020Quote: Fibroblasts were infected by Sendai virus(Thermo Fisher Scientific, A16517) Transfected fibroblasts (approximately 1*105 cells per nucleofection ...
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bioRxiv - Microbiology 2021Quote: ... 7μl of 4x Taqman Fast Virus Master Mix (Thermo Fisher), 5μl of DNAse/RNAse free water and 3μl of primers and probe mix (sequences shown in Table 1) ...
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bioRxiv - Immunology 2020Quote: ... the virus inoculum was replaced with fresh medium (MEM, Gibco) containing 1.25% Avicel® (FMC Biopolymer ...
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bioRxiv - Immunology 2021Quote: ... Virus was diluted in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) and maintained on ice prior to infection ...