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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and 1× Protein Thermal Shift dye (Applied Biosystems). The assay buffer (20 mM Tris pH 8.5 ...
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bioRxiv - Systems Biology 2023Quote: ... 1 mL of Protein G Dynabeads (ThermoFisher, #10003D) were washed once with 1X PBST (1X PBS + 0.1% Tween-20 ...
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bioRxiv - Genomics 2023Quote: 1 mL of Protein G Dynabeads (ThermoFisher, #10003D) were washed once with 1X PBSt (1X PBS + 0.1% Tween-20) ...
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bioRxiv - Molecular Biology 2024Quote: ... glial fibrillary acidic protein (GFAP; 1:50, Invitrogen), and EDC4 (1:40 ...
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Panc1 and MiaPaCa2 with RBFOX2 knockdown and non-targeting control were generated using human Affymetrix Clariom D Arrays (Life Technologies, Cat. no. 902922) with the standard input kit run on the Applied Biosystems GeneChip 3000 instrument ...
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bioRxiv - Neuroscience 2020Quote: ... and combined with 50μl 1× D-Luciferin Working Solution supplemented with 1× Firefly Signal Enhancer (Thermo Scientific Pierce Firefly Signal Enhancer ...
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bioRxiv - Genomics 2021Quote: ... iPSCs were treated with CNCC Derivation media: 1:1 Neurobasal medium/D-MEM F-12 medium (Invitrogen), 0.5× B-27 supplement with Vitamin A (50× stock ...
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bioRxiv - Neuroscience 2023Quote: ... A mixture of 1:1 Dulbecco’s modified eagle medium (DMEM) and Ham’s F12 (D/F medium, Gibco) supplemented with 10-15% Fetal Bovine Serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... iPSCs were cultured with CNCC specification media: 1:1 Neurobasal medium/D-MEM F-12 medium (Invitrogen), 1X penicillin-streptomycin solution ...
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bioRxiv - Bioengineering 2019Quote: ... were cultured in (DMEM containing D-glucose [1 g L-1] and sodium pyruvate [110 mg L-1] (Life Technologies), supplemented with penicillinstreptomycin [100 U mL-1] (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were then incubated for 1 hour with protein A-conjugated Dynabeads (Life Technologies), after which the beads were washed with lysis buffer at incremental KOAc concentrations (100 mM ...
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bioRxiv - Microbiology 2021Quote: ... 1 µl was used for protein quantification with the Pierce BCA Protein Assay Kit (Thermofisher). The remaining 9 µl were mixed with SDS (0.2% final) ...
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bioRxiv - Cancer Biology 2021Quote: ... the mixture of 2.5 μg of each lentiviral ORF expression plasmid and 0.25 μg of the Lenti-Pac HIV plasmid in 15 μL of EndoFectin Lenti was incubated with 200 μL of Opti-MEM® (Invitrogen). The DNA-EndoFectin Lenti complex was directly added into cultured cells before being allowed for overnight incubation at 37°C in a CO2 incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vector CSII-CMV-MCS-IRES2-Bsd harboring a cDNA was transfected with the packaging vectors pCAG-HIV-gp and pCMV-VSV-G-RSV-Rev into X293T cells using Lipofectamine2000 transfection reagent (Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: KBM7 (a kind gift from Thijn Brummelkamp) and Hap-Lat (latent HIV infected KBM7-derived cell lines) were cultured in IMDM media (ThermoFisher Scientifc) supplemented with 10% FCS and 2% Pen/Strep ...
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bioRxiv - Immunology 2020Quote: 293T cells for HIV pseudovirus production were obtained from the American Type Culture Collection (ATCC, #CRL-3216) and cultured in DMEM (Invitrogen, #10313021) supplemented with ...
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bioRxiv - Immunology 2021Quote: ... was synthesized from 10 µl of HIV RNA transcripts or extracted CA-RNA using Random hexamers and SuperScript IV Reverse transcriptase according to manufacturer protocol (Thermo Fisher). Next ...
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bioRxiv - Immunology 2021Quote: ... the PSG3 plasmid was co-transfected into HEK293T cells along with JRFL or YU2 HIV envelope plasmids using Lipofectamine 2000 transfection reagent (ThermoFisher Scientific) to produce single-round of infection competent pseudo-viruses representing multiple clades of HIV ...
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bioRxiv - Immunology 2023Quote: ... S-expressing cells were mixed at a 1:1 ratio with parental CEM.NKr CCR5+ cells (HIV Reagent Program #4376) and the target cell mixture was labelled with Aqua viability dye (Thermo Fisher Scientific) and eBioScience eFluor670 cell proliferation dye ...
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bioRxiv - Microbiology 2023Quote: HEK293T/17 (ATCC #CRL-11268) and TZM-bl cells (NIH HIV Reagent Program #ARP-8129) were cultured in DMEM complete media (DMEM (Gibco #11965092) supplemented with 10% FBS (Gibco #26140079) ...
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bioRxiv - Immunology 2023Quote: ... HIV reporter pseudoviruses expressing SARS-CoV-2 S glycoprotein and carrying the luciferase gene were produced in Expi293F cells (ThermoFisher Scientific) by co-transfection of the pNL4-3 ...
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bioRxiv - Immunology 2024Quote: ... Actinomycin D (ThermoFisher) was used to label dead cells ...
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bioRxiv - Immunology 2024Quote: ... Actinomycin D (Invitrogen) was added to label dead cells ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... 0.1 M NaCl and 1% n-Dodecyl β-D-maltoside (DDM, Anatrace, Affymetrix) and stirred for 18 hours for solubilization ...
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bioRxiv - Cancer Biology 2019Quote: ... or a non-targeting siRNA pool (Pool #1, D-001206-13, ThermoFisher Scientific). Efficiency of mRNA depletion was assessed 72 hours post-transfection using qPCR or western blot in the case of ACSL1 (Proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were as follows: HuC/D (mouse, ThermoFisher A21272, 1:200), Cherry (goat ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 mM 3’-d-GTP [>99% purity for UTP and CTP (ThermoFisher); ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase,” and thereby to stabilize ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-HuC/D (1:500; Cat#: A-21271, RRID:AB_221448; Thermo Fisher Scientific), rabbit anti-Krt5 (1:500 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 0.5% w/v Peptone (Formedium) and 1% w/v D-Glucose (Fisher Scientific). For solid medium ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal IgG2b anti-HuC/D (Invitrogen Thermo Fisher, A-21271, 1:250), Mouse monoclonal IgG1 anti-Phox2b (B-11 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal IgG2b anti-HuC/D (Invitrogen Thermo Fisher, A-21271, 1:250), Mouse monoclonal IgG1 anti-Phox2b (B-11 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and stained with 7-Aminoactinomycin D (7AAD) (Invitrogen, 1:400) that was used as dead cell marker ...
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bioRxiv - Bioengineering 2019Quote: MDA-MB-231 human breast cancer cells were cultured using 1:1 DMEM:F12 medium (Life Technologies) supplemented with 50 µg/ml gentamicin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Huh7 human hepatocellular carcinoma cells were grown in DMEM/F-12 1:1 medium (Thermo Fisher) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-Hec1 (1:1000, human monoclonal) and Anti-phospho-Paxillin (1:100, rabbit polyclonal, pTyr31, Invitrogen). Secondary antibodies diluted in antibody dilution buffer was added along with the conjugated Phalloidin-TRITC (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos were stained with mouse-anti-human-ZO-1 1:500 (Thermo Fisher Scientific, 33-9100) or guinea pig-anti-zebrafish-VE-cadherin 1:500 (Paatero ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Immunology 2019Quote: ... KIR2DL1*001-Fc, and KIR2DS4*001-Fc (R&D, 1844-KR, 1847-KR) were conjugated to protein-A Alexa Flour 647 (Invitrogen) by overnight incubation at 9:1 (molar ...
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bioRxiv - Neuroscience 2023Quote: ... coated 10-cm dishes at a density of 100,000 cells/cm2 or on coverslips coated by 20 µg/ml poly-D-lysine and 10 µg/ml laminin mouse protein (Gibco) at a density of 40,000 cells/cm2 in plating medium (high glucose DMEM medium containing L-glutamine and 10% fetal bovine serum) ...
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bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
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bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...
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bioRxiv - Neuroscience 2020Quote: ... monoclonal mouse anti-human α-synuclein (1:1000, 4B12, Thermo Fisher Scientific); the goat anti-ChAT (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... Human samples were embedded in M-1 embedding matrix (Thermo scientific, USA) and cut into 50 µm to 100 µm thick slices on a sliding freezing microtome (LeicaSM2010R ...
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bioRxiv - Immunology 2022Quote: ... Human Jurkat cells (Clone E6-1) were cultured in RPMI (GIBCO, 11875093) containing 10 % FCS and 1 % penicillin-streptomycin (cRPMI) ...
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bioRxiv - Neuroscience 2021Quote: ... or using goat anti-human IgG Alexa555 (Invitrogen, A-21433, 1:500) for Fc detection ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-human α-synuclein 1:1000 (Thermo Scientific; Rockford, IL, USA); goat anti-luciferase 1:5000 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunolabeled with rabbit anti-human LC3B (ThermoFisher L10382; 1:1000), 0.05% saponin at room temperature for 1h followed by Alexa FluorTM 647 goat anti-rabbit IgG (ThermoFisher A21245 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 × 106 primary hepatocytes (HEP10 Pooled Human Cryopreserved Hepatocytes; Thermo Fisher Scientific) were injected into the lower pole of the spleen.