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Citations for Thermo Fisher :
5901 - 5950 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The human PTH1R (residues 27-502) with G188A and K484R mutations was cloned into pFastBac vector (Invitrogen) with the haemagglutinin signal peptide (HA) ...
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bioRxiv - Neuroscience 2023Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... HCC1395 and SF295 human cancer cell lines were grown in RPMI-1640 (Thermo Fisher Scientific, Waltham, MA) medium supplemented with 10% FBS and 50 units/ml of penicillin/streptomycin.
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bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Exogenous mRNA levels of transgene expression human APOE (Hs00171168_m1) commercial TaqMan® primer/probe set (Applied Biosystems). Endogenous mouse Beta-Actin (Mm02619580_g1 ...
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bioRxiv - Immunology 2023Quote: The human promyelocytic leukemia HL-60 cell line (ATCC #CCL-240) was maintained in IMDM media (Gibco) with 10% heat-inactivated (HI ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with primary antibodies: rat anti-human Gal3 (Invitrogen #14-5301-82, 1:50 dilution) and mouse anti-human CD68 (Dako #M0814 ...
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bioRxiv - Bioengineering 2023Quote: Human foreskin fibroblasts (HFFs; CRL-2429, ATCC) and HFF-derived iPSCs were grown in Geltrex (Gibco, A1413302) coated 12-well plates and were fixed with 3.7% formaldehyde and then blocked with 3% BSA in 1X PBS for 1hr at Room Temperature prior to imaging ...
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bioRxiv - Immunology 2023Quote: The human myelogenous leukemia cell line K562 was cultured in Roswell Park Memorial Institute (RPMI) media (Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: Both plasmids encoding for the LC and HC were transfected into Expi293F human cells (Thermo Fisher Scientific) with purified DNA and polyethylenimine (PEI 1:3) ...
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bioRxiv - Cancer Biology 2023Quote: ... SW837 (human colon cells, ATCC CCL-235) were grown in Leibovitz’s L-15 medium (Gibco #11415-049) with 10% FCS (Hyclone #SH30084.03n ...
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bioRxiv - Cell Biology 2023Quote: Normal human epidermal keratinocytes (Kurabo) from Caucasian neonatal foreskin were cultured with EpiLife medium (Thermo Fisher Scientific) supplemented with 60 µM CaCl2 and HuMedia KG growth factor kit (Kurabo) ...
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bioRxiv - Genomics 2022Quote: ... CD4+ T cells were isolated by magnetic negative selection using Untouched Human CD4+ Isolation Kit (Invitrogen 11346D) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... Small interfering RNA was introduced into primary human umbilical vein ECs using the Neon transfection system (ThermoFisher). See Supplementary Table 5 for sources of siRNA ...
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bioRxiv - Microbiology 2023Quote: ... human actin mRNA and EMCV viral RNA was performed mixing the Fast SYBR green Master Mix (ThermoFisher) with cDNA and 1µM of the forward and reverse corresponding primers ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were incubated with PBS solution containing various concentrations of human plasma fibronectin (Thermo Fisher Scientific, US) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with HRP-coupled anti-human IgG (MilleniumScience) before adding the chromogenic substrate TMB (ThermoFisher). Reactions were stopped with 2 M H2SO4 and absorption measured at 450 nm.
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bioRxiv - Genomics 2023Quote: ... KRAB-dCas9 expressing cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D), at 1:1 cell:bead ratio ...
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bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated for 1 h with goat anti-human IgG Alexa Fluor 647 (Invitrogen) and goat anti-mouse IgG Alexa Fluor 488 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Human cervix adenocarcinoma epithelial HeLa 229 cells (ATCC CCL-2.1) were cultured in RPMI 1640 medium (Gibco) containing 5% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D737 infected erythrocytes were cultured in O positive human erythrocytes in RPMI 1640 media (Gibco, USA) containing 10% heat-inactivated pooled human sera and 5 μg/mL gentamicin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... version 46) and humans using SEQUEST and Mascot (Version 2.4.1) via Proteome Discoverer v2.2 (Thermo Fisher Scientific). Trypsin was selected as the protease of choice with a maximum of two missed cleavages ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 human monocytes (ATCC) were maintained in Roswell Park Memorial Institute 1640 Medium (RPMI 1640, Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with Hu5F9 or TJC4 and then stained with labeled anti-human secondary antibody (Invitrogen). B6H12 was acquired from Bio X Cell ...
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bioRxiv - Bioengineering 2023Quote: ... The tissues were then incubated with primary mouse monoclonal anti-human cardiac troponin T (Thermo Fisher Scientific) or mouse monoclonal anti-human CD3 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Gene expression profiling was performed using the Affymetrix GeneChip® Human Clariom S Array (Thermo Fisher Scientific), including more than 210,000 distinct probes representative of 21,448 annotated genes (Genome Reference Consortium Human Build 38 (GRCh38) ...
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bioRxiv - Microbiology 2023Quote: ... at an interval of 24 hr with siRNAs targeting human PKR/EIF2AK2 (Assay ID 142330-Thermofisher scientific) or human TIA-1 (Assay ID 139893-Thermofisher scientific ...
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bioRxiv - Neuroscience 2023Quote: Measurement of Aβ1-40 levels was performed using an Amyloid beta 40 Human ELISA Kit (Invitrogen, KHB3482). For further information please see Supplementary Material.
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from human lung ECs and mouse lung tissues using TRIZOL reagent kit (Invitrogen). The cDNA was generated using iScript cDNA synthesis kit (170-8891 ...
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bioRxiv - Immunology 2023Quote: ... A goat anti-Human IgG secondary antibody labelled with Alexa Fluor 488 was added (Thermo Fisher Scientific). The plates were incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Bound antibodies were detected with horseradish peroxidase (HRP)-labeled goat anti-human IgG secondary antibody (Invitrogen, A18817) at 37 °C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Anti-mouse ZAKa antibody (proteintech, #14945-1-AP) and anti-human ZAKa antibody were from Thermo Fisher Scientific (A301-993A) ...
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bioRxiv - Immunology 2023Quote: ... Cytokine release in the supernatants was measured with an Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen) and was analyzed with the DropArray (Curiox Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and S-D614G-N149Q were expressed in human Expi293F cells using the Expi293 Expression System (ThermoFisher Scientific) and purified using a HisTrap FF column (GE Life Sciences)(64 ...
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bioRxiv - Microbiology 2023Quote: ... Secreted antibodies were detected with 1µg/mL goat anti-human IgG (H+L)-Alexa Fluor 594 (Invitrogen), which was added to the cell culture media used to resuspend the antigen-coated beads.
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bioRxiv - Microbiology 2023Quote: ... and 50 ng of human ACE2-encoding plasmid or an empty plasmid control using Lipofectamine 2000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... human primary T cells and DG-75 cells were electroporated using the Neon Transfection kit (ThermoFisher Scientific) at 1600 volts / 10 ms / 3 pulses ...
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bioRxiv - Cell Biology 2023Quote: ... Human HK-2 cells were cultured in DMEM and Keratinocyte-SFM (1X) (Life Technologies Green Island NY) media ...
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bioRxiv - Cell Biology 2023Quote: ... Human primary keratinocytes were transfected at 20 nM final siRNA concentration using Lipofectamin 2000 reagent (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... and TOMM22) were amplified using an Ion AmpliSeq Transcriptome Human Gene Expression Core Panel (Thermo Fisher Scientific). Amplicons were ligated to barcode adapters and purified using Agencourt AMPure XP reagent (Beckman Coulter Inc.) ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were probed with rabbit monoclonal anti-human CD38 antibody (Clone RM388, Thermo Scientific, cat# MA5-36061) after blocking with 5% BSA ...
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bioRxiv - Cell Biology 2023Quote: Immortalized human retinal pigment epithelial cells (RPE-1hTERT, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK) 293T and Vero cells were grown in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (Thermo Fisher) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: A synthetic sequence of human Twisted gastrulation (residues Cys26-Phe223) codon optimised for mammalian expression (ThermoFisher Scientific) with a C-terminal TwinStrep-tag (IBA LifeScience ...
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bioRxiv - Biophysics 2024Quote: Human mesenchymal stem cells (hMSCs) (ATCC, lgc-standards, Teddington, UK) were cultured in high-glucose DMEM (Gibco) supplemented with 10% FBS and 1 % penicillin/streptomycin ...