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Citations for Thermo Fisher :
5851 - 5900 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Expi293F embryonic human kidney cells were maintained and transfected in Expi293(tm) Expression Medium (Thermo Fisher Scientific) as per the manufacturers’ protocol ...
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bioRxiv - Microbiology 2022Quote: ... uptake studies with TRITC-transferrin (Transferrin From Human Serum, Alexa Fluor™ 647 Conjugate #T23366, Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2022Quote: ... Cell count was estimated by qPCR using human copy number reference assay TERT (Applied Biosystems, cat# 4403316). Cell-associated HIV RNA was measured using the same primers in a one-step qPCR using TaqMan® RNA to Ct™ 1 Step kit (Applied Biosystems ...
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bioRxiv - Pathology 2022Quote: Mouse or human intestinal tissue was lysed in 100 μl ice-cold RIPA buffer (Thermo Fisher Scientific) with protease inhibitors (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Human hepatoma HC-04 cells were seeded in 384-well plates in DMEM/F12 culture medium (Gibco) supplemented with 10% heat inactivated fetal calf serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: HeLa cells (Human epithelial cells, ATCC CCL-2) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with heat-inactivated fetal calf serum (10%) ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were stained with the following fluorochrome-conjugated anti-human Abs: anti-CD4 (Invitrogen 17-0049-42), anti-FOXP3 (eBioscience 25-4777-61) ...
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bioRxiv - Biochemistry 2022Quote: Spectra were searched against the reviewed human database (November 2018) using Proteome Discoverer V2.2 Software (Thermo Fisher). Peptides were identified using a precursor mass tolerance of 10 ppm ...
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bioRxiv - Microbiology 2023Quote: ... The Calu3 cell line (human lung cancer cells) was preserved in Modified Eagle’s Medium (MEM, Gibco, USA) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Molecular Biology 2022Quote: 30mg of human brain tissue was homogenized in 1ml of TRIzol (Catalog No. 15596026, Thermofisher Scientific, USA). 200µl of chloroform was added to each sample and mixed well ...
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bioRxiv - Neuroscience 2022Quote: ... with GAPDH as housekeeping gene control (human GAPDH endogenous control, catalog#4325792, Life Technologies, Warrington, United Kingdom). The average of triplicate CT values from each sample was used to calculate the relative RNA levels (2-ΔCT) ...
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bioRxiv - Immunology 2022Quote: ... PCR products were then cloned into corresponding human IgG1 expression vectors with Gibson Assembly (Thermo Fisher Scientific) and the mixture was subsequently transformed into DH5α cells ...
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bioRxiv - Neuroscience 2024Quote: Elastase generated granulin peptides were obtained by incubation of rPGRN with human neutrophil elastase (# RP-77526, Invitrogen). 50 ng/µl PGRN and 80 ng/µl elastase were incubated in 100 mM sodium citrate pH 6.0 overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... 1:2000 dilution of secondary Alexa FLUOR-647 GOAT-Anti human IgG [H+L] (Invitrogen; #A-21445) was added to the cells for 30 minutes followed by 3 washes with FACS Buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Human iPSC spheroids were washed and growth factor-depleted using DMEM/F12 (Thermo Fisher Scientific #21331-046) for 2 h prior to stimulation ...
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bioRxiv - Systems Biology 2024Quote: Human osteosarcoma epithelial cells (U-2 OS cell line) were cultivated in DMEM high glucose medium (Gibco), supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary staining was done using a goat anti-Human Alexa Fluor (AF) 594 antibody (1:1000, Invitrogen), for 30 minutes ...
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bioRxiv - Pathology 2024Quote: Gene expression on human cells and tissue was quantified using TaqManTM assays (Fisher Scientific, Supplementary Table 3) and Luna® Universal qPCR Master Mix (M3003E ...
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bioRxiv - Pathology 2024Quote: ... and CXCL1 levels were measured in the supernatant of human precision-cut liver slices using ELISA (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Passage matched (±3) human induced pluripotent stem cells (hiPSCs) were cultured in StemFlex media (Life technologies #A3349401) on Matrigel (Corning ...
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bioRxiv - Systems Biology 2024Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) media (ThermoFisher, UK), supplemented with 10% Heat Inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... Since most present-day data was generated using different SNP arrays (Axiom LAT1 and Human Origin Affymetrix), this data was phased and imputed by array using TOPMed Michigan Imputation Server ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... left in resting state or activated with Dynabeads Human T-Activator CD3/CD28 (1161D, Thermo Fisher Scientific) for 72 h ...
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bioRxiv - Cancer Biology 2023Quote: ... SK-N-Be(2c) and SK-N-SH human neuroblastoma cells were cultured in DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: The human embryonic kidney cells (HEK293 and HEK293T) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic lung fibroblasts (MRC-5, European Collection of Cell Cultures) were maintained in DMEM (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: ... All human primary cell lines were cultured in DMEM high glucose supplemented with GlutaMAX (Gibco, 10566-016), with further addition of 15% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... followed by PBS washing and immunostaining with secondary AlexaFluor633 anti-human antibodies (Thermo Fisher Scientific, #A-21091) for 30 mins at 4°C in the dark ...
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bioRxiv - Cancer Biology 2024Quote: sEV isolated from human HTLV/T cell cultures with Total Exosome Isolation Reagent (#4478359, Thermo Fisher Scientific) were solubilized in SDS buffer ...
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney (HEK) 293T (#CRL-3216, ATCC) cells were maintained in DMEM GlutaMAX medium (#61965026, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (#15140122 ...
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bioRxiv - Biochemistry 2024Quote: ... human full-length VPS35L (untagged, synthesized as a codon-optimized GeneString from Thermo Fisher to improve expression), VPS26C (untagged) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation with an anti-human Alexa Fluor 488 conjugate (1:200, A-10631, Thermo Fisher). For comparing tumor cell growth in different groups ...
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bioRxiv - Cancer Biology 2024Quote: ... and cDNA hybridized to a Mouse Clariom S Human Transcriptome Assay microarray plate (Affymetrix, Santa Clara, CA) and the plate was scanned by a GeneTitan MC Instrument (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Immunology 2023Quote: Immortalized human SZ95 sebocytes (Zouboulis et al., 1999) were cultured in T-75 culture flasks (Thermo Scientific) with Sebomed basal medium (DMEM/F12 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once and then incubated with Alexa Fluor 488 goat anti-human IgG antibody (ThermoFisher) diluted 1/200 in PBS + 1% FCS or BB buffer ...
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bioRxiv - Cell Biology 2023Quote: The release of CXCL10 to the culture medium was detected using Human ProcartaPlex immunoassays (Invitrogen, Vienna, Austria) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... Human embryonic kidney 293T cells were grown in Complete DMEM (Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning) ...
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bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... 8 µg of cDNA was fragmented and labeled using GeneChip Human Mapping 250K Nsp Assay Kit (Affymetrix), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Hepatic gene expression was measured using Affymetrix Human Exon 1.0 ST v1 Array (Affymetrix, Santa Clara, CA) in adolescents and Illumina Human-6 Expression Bead Chips (Illumina ...
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bioRxiv - Bioengineering 2022Quote: Intracellular mitochondrial presence within human heart organoids was visualized using Mitotracker Deep Red FM (Thermo Fisher Scientific). Mitotracker was prepared according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Calcium transient activity within the human heart organoids was assessed using Fluo-4 AM (Thermo Fisher Scientific). Fluo-4 AM was solubilized in DMSO per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs, ATCC SRC-1041) maintained in DMEM (GIBCO 11965118) supplemented with 3% inactivated fetal calf serum and 10 µg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Fibroblasts were isolated from human skin biopsies and were cultured in DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% P/S ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were activated with 30 U/mL rIL-2 and Dynabeads Human T-activator CD3/CD28 (ThermoFisher) and cultured with the CM of CA-MSCs in the presence or not of CXCR4 Ab (R&D Systems) ...