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Citations for Thermo Fisher :
5801 - 5850 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: MCF10A human mammary epithelial cells (ATCC) were grown at 37° and 5% CO2 in DMEM/F12 (Gibco) with 5% horse serum (HS ...
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bioRxiv - Cell Biology 2020Quote: Control human fibroblasts were transfected with 12 nM EXD2 siRNA (Dharmacon) using 2.5 µl Lipofectamine RNAiMAX (Invitrogen) according the manufacturer’s instructions using a reverse transfection procedure in a 6-well plate ...
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bioRxiv - Cancer Biology 2020Quote: ... CNVs were provided based on calls from SNP microarray data (Affymetrix Genome-Wide Human SNP Array 6.0) and the GISTIC algorithm [35] ...
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bioRxiv - Microbiology 2021Quote: ... transformed into MaV203 and used as a bait to screen a human embryonic brain cDNA library (Invitrogen). Media ...
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bioRxiv - Cancer Biology 2021Quote: ... Two sets of primers were used: Megaplex RT Primers Human pool A and B (Thermo Fisher, USA). Together ...
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bioRxiv - Genomics 2020Quote: Human BUD13 was cloned from HeLa cDNA into the pCR 2.1-TOPO vector (Thermo Fisher Scientific, 450641). After sequence confirmation ...
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bioRxiv - Microbiology 2020Quote: ... human glioma cell lines U87 and U251 were all cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... human NLRP1 TEV or mouse NLRP1B were cloned into the pcDNA5/FRT/TO backbone (Invitrogen, Carlsbad, CA) with an N-terminal 3xFlag and mCherry tag ...
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bioRxiv - Microbiology 2020Quote: ... for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen, Thermofisher Scientific) for dsRNA detection ...
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bioRxiv - Microbiology 2020Quote: ... for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen, Thermofisher Scientific) for dsRNA detection ...
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bioRxiv - Microbiology 2020Quote: ... the coding sequence of SC was optimized for expression in human cell lines and synthesized by ThermoFisher Scientific (Waltham ...
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bioRxiv - Microbiology 2020Quote: ... after which it was replaced by 100μl 2μg/ml Alexa Fluor 647 Goat Anti-Human IgG (ThermoFisher) plus 2μg/ml DAPI (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: Fresh PBMC were used to isolate primary monocytes using Dynabeads® Untouched™ Human Monocytes (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with Alexa Fluor 594 conjugated goat anti-human IgG antibodies (Invitrogen, Thermo Fisher Scientific) for 45 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with Alexa Fluor 594 conjugated goat anti-human IgG antibodies (Invitrogen, Thermo Fisher Scientific) for 45 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Wells were then incubated with a secondary goat anti-human IgG labelled with horseradish peroxidase (HRP) (Invitrogen) or with a rabbit polyclonal anti-human IgA alpha-chain labelled with HRP (Abcam ...
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bioRxiv - Immunology 2020Quote: ... neck and CRD regions of human SP-D was transformed into Escherichia coli BL21 (λDE3) pLysS (Invitrogen). The transformed colonies (selected by ampicillin resistance ...
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bioRxiv - Microbiology 2021Quote: A549 human epithelial cells were cultured as described by the supplier (ATCC) in Ham’s F12 media (Gibco) + 10% FBS (VWR) ...
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bioRxiv - Immunology 2020Quote: HEK 293-F cells (RRID:CVCL_D603; originally derived from female human embryonic kidney cells) were obtained from Invitrogen (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... flow cytometric analysis was performed using rabbit anti-human ANXA1 (Thermo Fisher Scienific, Ulm, Germany, 1:100) and Alexa Fluor 647-conjugated goat anti-rabbit IgG H+L (Invitrogen ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Immunology 2020Quote: ... 50 μL of a secondary Alexa Fluor 647 goat-anti human antibody (1:500) (Life Technologies A21455) was used ...
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bioRxiv - Immunology 2020Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... The transformed human bronchial epithelial cell line BEAS-2B (ATCC) was maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Medium or DMSO and Dynabead™ Human T-Activator αCD3/αCD28 (αCD3/αCD28) (Cat# 11132D, Gibco™) stimulated cells were used as controls ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with Alexa Fluor 594 conjugated goat anti-human IgG antibodies (Invitrogen, Thermo Fisher Scientific) for 45 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with Alexa Fluor 594 conjugated goat anti-human IgG antibodies (Invitrogen, Thermo Fisher Scientific) for 45 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The remainder was stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA (Fraction V ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 human breast cancer adenocarcinoma cells (ATCC) were cultured in high-glucose DMEM (Thermo Fisher) supplemented with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Cancer Biology 2022Quote: ... for 293T internalization studies and goat anti-rabbit-Alexa-488 and goat anti-human-Alexa-555 (Invitrogen) for lysosome colocalization studies ...
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bioRxiv - Cancer Biology 2022Quote: ... The human MDA-MB-231 and JIMT1 breast cancer cell lines were cultured in DMEM medium (Gibco) supplemented with 10% FBS and 1% Pen-Strep (Gibco).
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney cells (HEK293T, ATCC) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... Human ADARB1 and ADARB1(+5a) were amplified from Jurkat T cell cDNAs and cloned into pcDNA3 (Invitrogen). HEK293 cells were transfected with the control and ADARB1 encoding pcDNA3 constructs using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: The human PTH1R (residues 27-502) with G188A and K484R mutations was cloned into pFastBac vector (Invitrogen) with the haemagglutinin signal peptide (HA ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were incubated overnight at 4 ºC in primary mouse anti-human αSMA monoclonal antibody (Fisher Scientific catalog no.
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bioRxiv - Cancer Biology 2022Quote: Day (D) 13-14 differentiated CD34+-derived human megakaryocytes were washed in Phosphate-Buffered Saline (PBS, Gibco), and then resuspended as previously described to study agonist responsiveness30 ...
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bioRxiv - Biochemistry 2022Quote: Human umbilical vein endothelial cells (HUVECs) were maintained in culture in RPMI 1640 medium (Invitrogen, Life Technologies) supplemented with 10 % heat-inactivated fetal bovine serum (Invitrogen Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: Human umbilical vein endothelial cells (HUVECs) were maintained in culture in RPMI 1640 medium (Invitrogen, Life Technologies) supplemented with 10 % heat-inactivated fetal bovine serum (Invitrogen Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: The assay was performed using the 1-Step Human Coupled IVT Kit (# 88882, Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions using ‘turbo-type’ green fluorescent protein (tGFP ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with anti-mouse/human CD309 (Flk-1/VEGFR2)-APC (Thermofisher, 17-5821-81; 1:100) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and plated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (ThermoFisher) at a 2:1 ratio for 48 hours ...
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bioRxiv - Genomics 2022Quote: Human CAFs and normal fibroblasts were transfected with siRNA using the Lipofectamine® RNAiMAX Reagent kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... rs7069590 and rs2435357 using specific TaqMan Human Pre-Designed genotyping assays following the manufacturer’s protocol (ThermoFisher Scientific). The assays IDs are C_26742714_10 ...
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bioRxiv - Genetics 2022Quote: ... A mix of 6 ul of nick translated RNA probe with 12ul of Human Cot-1 (ThermoFisher) and 2ul of salmon testes (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: Adiponectin concentration in the conditioned medium was measured using the Adiponectin Human ELISA Kit (Cat# KHP0041, ThermoFisher) with a Safire 2 microplate reader (Tecan).
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bioRxiv - Molecular Biology 2022Quote: ... and were stimulated for two days prior to electroporation with anti-human CD3/CD28 magnetic dynabeads (ThermoFisher) at a beads to cells concentration of 1:1 ...
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bioRxiv - Microbiology 2022Quote: ... Human colon cancer cell line HT29 and HCT116 were cultured in DMEM/F-12 HEPES (GIBCO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: Human lung epithelial (A549) cells and African green monkey kidney (Vero) cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: ... human embryonic kidney 293T and Vero cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Gibco), supplemented with 100 U/mL penicillin ...
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bioRxiv - Microbiology 2022Quote: ... mNAb binding was detected using horseradish peroxidase–labelled goat anti-human IgG F(ab’)2 (Thermofisher-Scientific), or horseradish peroxidase–labelled anti-human IgA ...