Labshake search
Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... the DMEM+ was replaced with 0.5 mL of neurobasal medium (NBM: 2% B27, Invitrogen; penicillin/streptomycin; 2 mM α-glutamine; in neurobasal medium A, GIBCO). An additional 0.5 mL/well of NBM was added at 3 days in vitro (DIV) ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting peptic peptides were collected on a trap column (2 mm x 2 cm; 0.5 °C) filled with POROS 20 R2 (ThermoFisher Scientific). After 3 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... in facility water (2-month-old fish) or an E3 solution supplemented with 0.2 mM 1-phenyl-2-thiourea (PTU; Acros Organics) (larvae) ...
-
bioRxiv - Bioengineering 2023Quote: ... MDA-MB-231 cells were plated as above and treated with 100 nM of Cy5-labeled si<(EG18L)2 or si<(EG18Ldiacid)2 in Opti-MEM (Gibco) for 2 h at 37°C vs ...
-
bioRxiv - Synthetic Biology 2023Quote: ... in Tris-Glycine SDS Running Buffer (225V, 45 min)and transferred to nitrocellulose (iBlot 2, iBlot 2 NC Mini Stacks; Invitrogen) at 20 V for 1 min ...
-
bioRxiv - Cell Biology 2023Quote: ... In brief pulse labeling with FM4-64 (2 μM FM464, Molecular Probes; made from a 2-mM stock in DMSO) was done for 5 min (time 0 ...
-
bioRxiv - Pathology 2023Quote: ... Peptides were eluted under a 60min 2%-37% acetonitrile gradient for mouse degradome (2%-45% for human degradome) and transferred by Nanospray Flex nanoelectrospray source (ThermoFisher) into an Orbitrap Elite - ETD mass spectrometer (ThermoFisher ...
-
bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... Primary T lymphocytes stimulated with PHA and IL-2 (2 × 105, 100 µl/well) were resuspended in HBSS buffer (Gibco) containing 0.1% (w/v ...
-
bioRxiv - Cell Biology 2023Quote: Plasma from LPS-treated mice were diluted 1:10 with Hanks’ Balanced Salt Solution (no Ca+2/Mg+2; Gibco) in 96-well black MaxiSorp™ plate ...
-
bioRxiv - Immunology 2023Quote: ... then incubated in wash buffer (Advanced DMEM, 10% FBS, 2 mM L-glutamate, penicillin-streptomycin) with 2 mg/mL collagenase type I (Invitrogen) at 37°C for 5-15 mins with vigorous mixing as previously described.57 Crypts were plated in Matrigel with Human Organoid Growth Media (StemCell) ...
-
bioRxiv - Bioengineering 2023Quote: ... Cetuximab and Avelumab F(ab’)2 fragments were generated using the Pierce F(ab’)2 preparation kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... FT352-2 and MLA18-2 without stop codons and insect-cell codon-optimized Sr50 were obtained by gene synthesis (GeneArt, Invitrogen). Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cell populations were imaged in media at the designated time points at 10x magnification and 2×2 binning using the ArrayScanTM XTI High Content Reader (ThermoFisher). Tomato MFI and EGFP MFI was imaged with an excitation source of 560/25nm and 485/20nm respectively ...
-
bioRxiv - Neuroscience 2023Quote: ... the pellet was washed with HBSS and then resuspended in 10 mL magnetic-activated cell sorting (MACS) buffer (Hibernate-A with 2 mM sodium pyruvate, 2% B27, 0.5% BSA; ThermoFisher Scientific), 2 mM EDTA (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: Monolayers of HEK293T cells cultivated on coverslips were co-transfected with equimolar amounts of SIDT1/2-GN and SIDT1/2-GC plasmids using Lipofectamine 3000 (Invitrogen). After 24 hours of transfection ...
-
bioRxiv - Immunology 2023Quote: ... samples were washed 3X in PBS before incubating 2 hours at room temperature in 2 μg/ml donkey anti-rabbit IgG conjugated to AF594 (CAS: A21207, Thermofisher). After 3 washes in PBS ...
-
bioRxiv - Bioengineering 2024Quote: ... 2 µL of Golden Gate reaction were added to 25 µL of 2× Phusion Polymerase Master Mix (ThermoFisher, Cat# F531L), mixed with 18 µL of H2O ...
-
bioRxiv - Molecular Biology 2024Quote: ... co-transfection of pX-CRISPR and qTAG repair plasmids was performed at a 1:1 ratio using 4 μg of total DNA (2 μg cut + 2 μg repair) in Opti-MEM Reduced Serum Medium (31985070, Gibco) with Lipofectamine 3000 (L3000008 ...
-
bioRxiv - Neuroscience 2024Quote: ... and EGFP with 2:2:1 (total 0.5 µg / coverslip) using the Lipofectamine 3000 transfection reagent kit (Thermo Fisher Scientific). Transfected cells were identified by EGFP signals ...
-
bioRxiv - Immunology 2024Quote: ... Yeast was routinely grown in YPD broth (2% peptone [Fisher Scientific], 2% glucose [Fisher Scientific], and 1% yeast extract [Sigma]) at 30°C for 18 hours at 200rpm ...
-
bioRxiv - Immunology 2024Quote: ... permeabilised with 0.5% Triton x-100 and OPP incorporation into newly synthesised peptides was measured by conjugating with Alexa647-azide via a Click-IT chemistry reaction (2 µM Alexa-647-azide, 2 mM CuSO4, 5 mM ascorbic acid in PBS) (Invitrogen) and assessing fluorescence by flow cytometry.
-
bioRxiv - Genetics 2024Quote: Metabolites from LB broth were extracted via the addition of ice cold 2:2:1 (v/v) mixture of acetonitrile:methanol:water (#A456, #A955, #W6, respectively; Thermo Fisher Scientific) at a ratio of 30 µl sample per mL of extraction solvent ...
-
bioRxiv - Biophysics 2024Quote: ... were seeded in a 6-well culture plate at a concentration of 2 x 105 cells ml-1 (2 ml per well in DMEM (Nissui) supplemented with 5 % fetal bovine serum (Gibco), glutamine ...
-
bioRxiv - Bioengineering 2021Quote: 500 μg of RNA was extracted from hBMMSC-seeded ADC gels (n = 3) using TRIzol reagent (Invitrogen, USA) as per manufacturer’s instructions following the initial 48 h of mechanical stimulation ...
-
bioRxiv - Bioengineering 2021Quote: ... 10 μg of extracted proteins (n = 3) were solubilized in 1 × NuPage® LDS Sample buffer (Invitrogen, USA) and was run in a 15 well 12% Tris-Gly precast gel (Invitrogen ...
-
bioRxiv - Genetics 2020Quote: mRNA was collected from groups of 10 whole larvae (n=3–4 replicates per line) using Trizol (ThermoFisher) and reverse transcribed to cDNA using SuperScript III Reverse Transcriptase (ThermoFisher) ...
-
bioRxiv - Microbiology 2021Quote: ... the drugs were diluted from 0.78-25µM in 200µL of MOPS [3-(N-morpholino) propanesulfonic acid] buffered RPMI 1640 (Life Technologies), pH 7 ...
-
bioRxiv - Immunology 2024Quote: ... or 48 hours post stimulation in the presence of 2.5μM IMD0354 (n=3) were collected into TRIzol reagent (15596026, ThermoFisher). RNA isolations were performed according to the manufacturers protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Isopropyl-β-D-thiogalactopyranoside (IPTG) and 3-(N-morpholino) propane sulfonic acid (MOPS) were purchased from Fisher Scientific. MTSES was purchased from Biotium.
-
bioRxiv - Neuroscience 2024Quote: ... or RGS14 (n=3, 1:500, mouse anti-RGS14 #75-170, Neuromab)/goat anti-mouse IgG2a (Life Technologies).
-
bioRxiv - Neuroscience 2021Quote: ... or rabbit IgG antibody (2 μg, Thermo Fisher), as well as anti-S ...
-
bioRxiv - Cancer Biology 2021Quote: ... EdU 10μM (5-ethynyl-2’-deoxyuridine, ThermoFisher Scientific) diluted in fresh medium was added in the well for 10 minutes (MIA PaCa-2) ...
-
bioRxiv - Cancer Biology 2021Quote: ... For GSE7127 (Affymetrix U133 Plus 2 microarray platform), the data was normalised using Robust Multichip Average (RMA ...
-
bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-glutamine (Life Technologies #25030-024), 1x N2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 2 mM L-glutamine (Thermofisher, 25030-081). Spheroids were allowed to grow for up to 72-96h after which they were dissociated and treated as a monolayer ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2-β-mercaptoethanol (Gibco - catalogue no. 31350-010) and L-glutamine ...
-
bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Thermo Fisher; 25030024). Dissociated cortical cells from E18 Sprague Dawley rat pups were plated in 35 mm glass bottom dishes (MatTek ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 mL TrypLE trypsin (Gibco, Catalog No. 12563011) was then added to the plate and incubated for 3 min at 37 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... B-27 Supplement (2%, Thermo Fisher Scientific, 17504044), GlutaMax (1% ...
-
bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Thermo Fisher Scientific) with Sonic hedgehog (Shh-C24II ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 μl 0.1 M DTT (Thermo Fisher Scientific) and 1 μl RNaseOUT (40 units/μl ...
-
bioRxiv - Cell Biology 2020Quote: ... Beta-2-Microglobulin-Mm00437762_m1 (Applied Biosystems, Waltham, MA). Gene expression levels were normalized to β-2-microglobulin ...
-
bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2020Quote: ... and 2% B27 (all reagents from Life Technologies)] at 3.4 × 104 cells/cm2 on poly-D-lysine-coated (Sigma) ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific 21985023), 2 mM L-alanyl-L-glutamine (Thermo Fisher Scientific 35050079) ...
-
bioRxiv - Cell Biology 2020Quote: ... in chloroform/methanol/propan-2-ol (Thermofisher Scientific) (1:2:4 V:V:V ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 2 mM essential amino acids (Invitrogen), 10 units.ml−1 penicillin ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 mM L-Glutamin (Gibco, Thermo Fisher, USA), and 1 mM Sodium pyruvate (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... 2% (v/v) B27 Supplement (Thermo Fisher; 17504044), and 2 mM L-glutamine (Thermo Fisher ...