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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... embryos were collected and brains were removed in ice-cooled Ca+2/Mg+2-free Hank’s balanced salt solution (Gibco) with 10mM HEPES (Thermofisher) ...
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bioRxiv - Immunology 2022Quote: ... GATA-2-expressing vector was constructed by cloning synthesized mouse GATA-2 sequences into eukaryotic expression vector pcDNA3.1 (+) (Invitrogen) with restriction enzymes of NheI and EcoRI (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... both somal and axonal compartments were incubated with 2 μM of the calcium indicator dye Fura-2-AM (Invitrogen) in imaging buffer consisting of HBSS [Ca2+ and Mg2+-free] supplemented with 10 mM HEPES (ThermoFischer) ...
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bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
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bioRxiv - Microbiology 2021Quote: Vero cells were seeded at 1.0 × 104cells per well with Dulbecco’s modified Eagle’s medium (DMEM; Welgene) supplemented with 2% heat-inactivated fetal bovine serum (FBS) and 2% antibiotic-antimycotic solution (Gibco) in a black ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed twice with 2 ml Neurobasal supplemented with 2% B27 and 0.5 mM L-glutamine (all from Gibco), and dissociated by 8–10 bouts of trituration ...
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bioRxiv - Cell Biology 2021Quote: ... and 2+2 μg of plasmid DNA encoding the proteins were mixed in Opti-MEM™ (Thermo Fisher Scientific) and applied onto the cells in a single well ...
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bioRxiv - Neuroscience 2019Quote: ... 2 μl of fluorescence Alexa-594 conjugated cholera toxin β subunit (CTB) (2 μg/μl, Invitrogen; Carlsbad, CA, USA) was micro-injected into the vitreous body using a Picospritzer III (Parker Inc. ...
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bioRxiv - Bioengineering 2020Quote: ... the cell pellets were incubated for an hour in 2 uM 2’,7’-dichlorodihydrogen-fluorescein diacetate (H2DCF-DA, Invitrogen) in phenol red free media (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... counter stained with 2 mg/mL Alexa Fluor® 594 F(ab’)2 goat anti-mouse IgG (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... the dorsal cord was washed twice with 2 ml Neurobasal supplemented with 2% B27 and 0.5 mM L-glutamine (all from Gibco) and dissociated into single cells by 8–10 bouts of trituration ...
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bioRxiv - Microbiology 2020Quote: ... were seeded with 3.0 x 105 /ml Vero E6 cells in 0.5 ml volumes of 2 x MEM medium containing Glutamax (2 x MEM Gibco) + 10% FCS (Sigma, ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cultures were concentrated roughly four-fold by centrifugation (2 min 2000 xg) and 2 μl cells were placed on a slice of 1.5% UltraPure agarose (Invitrogen) dissolved in minimal media ...
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bioRxiv - Neuroscience 2022Quote: [Ca2+]c was measured by ratiometric analysis using acetoxy-methyl-ester Fura-2 (Fura-2/AM; F1221 Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... as above and left to attach for 2 hours before adding bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrDU, Invitrogen B23151) to a final concentration of 75 µM and left to proliferate for 6 hours ...
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bioRxiv - Physiology 2022Quote: Constructs for F4-2_Fab (hereafter F4-2) and L6-F4-2 were cloned into the pcDNA3.1(+) mammalian expression vector (Thermo Fisher). The F4-2 heavy chain contains a 6xHis tag on its C-terminus ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs-derived neuronal cells were incubated with the cell-permeant Fura-2 acetoxylmethylester (2 μM; Molecular probes, Life technologies) for 1 h at 37 °C in culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs-derived neuronal cells were incubated with the cell-permeant Fura-2 acetoxylmethylester (2 μM; Molecular probes, Life technologies) for 1 h at 37 °C in culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... Interleukin-2 (IL-2; Clinigen, US-CA) was diluted in phosphate buffered saline (PBS; Thermo Fisher Scientific, US-CA) and added at a final concentration of 300 I.U./mL ...
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bioRxiv - Cell Biology 2024Quote: ... as previously described.26 The platelet pellet was resuspended in HEPES-buffered Tyrodes buffer with BSA 0.35% (w/v) containing 2 µM fura-2-AM (Invitrogen), and incubated for 45 minutes in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CM for 48 hours before loading with 2 μM fura-2 acetoxymethyl ester (Molecular Probes) in calcium imaging buffer (CIB ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the animal’s brain was extracted and the meninges removed in a Petri dish containing cooled HBSS +/+ (Hank’s Balanced Salt Solution with Ca 2+ and Mg 2+: Gibco) to maintain their metabolism ...
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bioRxiv - Physiology 2023Quote: ... Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester) acquired from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2023Quote: ... Two microliters of culture were spotted onto a 2 cm x 2 cm pad containing 1.5% UltraPure agarose (Invitrogen) + either BG-11 (for Se ...
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bioRxiv - Biochemistry 2023Quote: ... onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5 sec blot time at 100 % humidity and 22°C ...
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bioRxiv - Genomics 2023Quote: ... Cells were blocked for 1-2 hrs using 2% (v/v) Bovine Serum Albumin (BSA, Thermo Scientific, Cat # 37525) + 5% FBS in HBSS ...
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bioRxiv - Molecular Biology 2024Quote: Vero E6 cells growing in P24 wells were infected with SARS-CoV-2 (Wuhan-1) at a moi = 2 pfu/cell in 0.5 mL OPTIMEM (Gibco) under biosafety level 3 conditions ...
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bioRxiv - Immunology 2024Quote: ... the inoculum was replaced with 150 μL of overlay medium (1:1 mix of Leibovitz’s L-15 medium supplemented with 0.1% pen/strep, 2% TPB, 1× NEAA, 2× Antibiotic-Antimycotic [Life Technologies] ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 3 mins at 90C and separated on a 6% denaturing PAGE gel (Invitrogen). The RNA was then transferred to a HyBond-N+ nylon membrane (GE Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were injected into a C-18 trap column (Acclaim PepMap100, 75 μm i. d. x 2 cm, C18, 3 μm, 100 Å, Thermo Fisher Scientific) and further separated on a C-18 analytical column (Acclaim PepMap100 ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS and incubated for 2-3 hr in secondary antibodies (conjugated to Alexa-488, Alexa-568, and Alexa 647, Life Technologies,1:500) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Developmental Biology 2020Quote: ... Rehydrated embryos were blocked with 10% BSA (in PBST) for 2-3 hours and incubated with anti-GFP antibody (1:1000; ThermoFisher Scientific, A-11120) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 100 ng/ml LPS for 3 hr followed by incubation with 2 µM Mitosox red (Thermo Fisher Scientific, M36008) for 30min ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells were seeded into 12-well plates and were transfected with 1.5 μg the RPL plasmid and 0.5 μg Cas13b gRNAs (NTC, U1-1, U1-2, U1-3) while ∼80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, Cat.# L3000015). For RIP experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... They were maintained on irradiated mouse embryonic fibroblast feeder cells (p2 DR4 expanded MEFs from WT-MRC Cambridge Stem Cell Institute) and were passaged every 2-3 days using TrypLE (Thermo Fisher Scientific, 12605028). ROCK inhibitor (10μM ...
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bioRxiv - Cancer Biology 2019Quote: An aliquot of each final fraction was loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Systems Biology 2019Quote: The 72 fractions obtained from the OG fractionation (3 TMT x 24 fractions) were loaded on a trap nanocolumn (0.01 x 2 cm, 5 μm; Thermo Fisher Scientific, Massachusetts, USA) and separated with a C-18 reversed-phase (RP ...
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bioRxiv - Biochemistry 2019Quote: ... concentrated and washed for 3 min on a C18 reverse phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm length, Thermo Fisher Scientific). Peptides were separated on a C18 reverse phase resin (2 μm particle size ...
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bioRxiv - Cancer Biology 2020Quote: ... an aliquot of each enriched sample was injected onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, 3 μm, 100 Å, Thermo Scientific) coupled to an analytical column (EASY-Spray PepMap column ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from 2×106 cells (3 biological replicates for wild-type and CNOT3-DM cells) using Trizol reagent (Thermo Fisher Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... SDS-Page separation was completed by running 2 μg of total protein on a NuPAGE™ 3-8% Tris-Acetate gel (Thermo Fisher Scientific). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... and loaded onto a reverse phase trap column (Acclaim PepMap 100, 75µm x 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, U.S.A.) using an Ultimate 3000 for 50 µL at a flow rate of 10 µL min-1 ...
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bioRxiv - Immunology 2021Quote: ... Peptides were initially trapped on a 2 cm long trap column (Acclaim PepMap100, 3 μm C18 particle size, 100 Å pore size, 75 μm inner diameter, Thermo Fisher Scientific) for 10 min at a flow rate of 6 μl/min with 0.1 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid ...
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bioRxiv - Plant Biology 2021Quote: ... Five microliters of peptide mixtures were loaded onto an Acclaim PepMap™ precolumn (75 μm × 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm × 50 cm ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated in blocking buffer with 8 ng/μL of custom labeled secondary antibodies or of commercial IgG-gam-F(ab’)2-Alexa Fluor 647 (DOL ~ 3) (Thermo Fisher, A-21237) for 45 min ...