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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The samples were harvested and fixed with 3 % PFA for 15 minutes and then stained with 2 μM of CellTracker™ fluorescent probes (ThermoFisher Scientific #C34552). Z-stacks were taken using a Leica TCS SP5 Matrix confocal microscope with the 20X objective ...
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bioRxiv - Cell Biology 2020Quote: Fibroblasts were plated in 96-well plates (10,000 fibroblasts/well) for 3 days in 2% FBS in DMEM supplemented with ascorbic acid (50 μg/mL) (Fisher Scientific, Waltham, MA, USA). Fibroblasts and matrix were then fixed and immunostained as detailed above ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA oligonucleotides were obtained as pre-designed siRNAs as follows: MFF-sense strand: 5’-CGCUGACCUGGAACAAGGAdTdT-3’ for exon 2 30 (Ambion, Austin, TX, USA); DLP1-sense strand ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were loaded onto an Acclaim Pepmap 100 C18 trap column (75 μm x 2 cm nanoViper, 3 μm, 100 Å) (Thermo Fisher Scientific) at 2 μL/min ...
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bioRxiv - Neuroscience 2022Quote: ... free floating NAc sections were first washed (3 × 5 min) in 1x PBS containing 2% Triton X-100 (PBST) (Thermo Fisher, Waltham, MA). Sections were then blocked in 5% normal goat serum (NGS ...
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bioRxiv - Microbiology 2021Quote: ... The phospholipolytic activity was measured according to 83 using a synthetic fluorescent substrate 1-palmitoyl-2-(10-pyrenyldecanoyl)-sn-glycero-3-phosphocholine (Molecular Probes, The Netherlands) and a Hitachi F-4000 spectrofluorimeter.
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Evolutionary Biology 2022Quote: ... through PCR amplification using pair of primers 2 and 3 (Table S1) with a 2X Phusion Flash PCR Master Mix (Thermo Scientific, Cat. # F548S). Gibson Assembly (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried peptides were resuspended with 0.1%FA and 500ng peptides were loaded on a HPLC-column (75-μm inner diameter, 2-cm length, 3-μm particle size, 100-Å pore size, Thermo Fisher Scientific) at a flow rate of 2 μL/min ...
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bioRxiv - Microbiology 2020Quote: ... The peptide samples were loaded (2 µl) onto a C18-reversed phase Acclaim PepMap 100 trap column (2 cm x 75 µm, 3 µm particles; Thermo Fisher Scientific, USA) with 30 µL of buffer A (2% v/v acetonitrile ...
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bioRxiv - Biochemistry 2019Quote: ... The mixture was then alkylated using 3 µL of 400 mmol/L 2-chloroacetamide (CAA, Thermo Scientific; final concentration of 40 mmol/L) and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Systems Biology 2019Quote: ... the peptides were loaded onto a Thermo Acclaim Pepmap (75 μm inner diameter with 2 cm length, C18, 3 μm particle size, 100 Å pore size; Thermo Fisher Scientific) pre-column and onto an Acclaim Pepmap Easyspray (75 μm inner diameter with 15 cm length ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap column (75 μm inner diameter × 2 cm, 3 μm, 100 Å; Thermo Fisher Scientific) for 3.5 min at 5 μL/min with 2% acetonitrile (ACN) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific, 12321D) and washed twice with TBS+NP40 wash buffer ...
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bioRxiv - Molecular Biology 2019Quote: The 2′ O-methyl phosphorothioate AOs were transfected into dermal fibroblasts as lipoplexes using 3 μl of Lipofectamine 3000 (Life Technologies, Melbourne, Australia) per 1 ml of OptiMEM ...
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 3 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL min−1 for 3 min ...
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E-cadherin regulates the stability and transcriptional activity of β-catenin in embryonic stem cellsbioRxiv - Cell Biology 2021Quote: ... Cells were replated at a density of 2×103 cells/cm2 every 3 days after dissociation with 0.25% trypsin–EDTA (1X) (Gibco, cat no. 25200-056). Cells were grown in Knockout DMEM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... An adenylated adaptor was ligated to the 3’-end of the captured transcripts (Supplementary Table 3) by using a mixture of T4 RNA ligase and truncated T4 RNA ligase 2 (ThermoFisher Scientific and NEB), which served as a template for the reverse transcription primer (Supplementary Table 3) ...
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bioRxiv - Genetics 2021Quote: ... Five microliters of peptide mixture were loaded onto an Acclaim PepMap™ precolumn (75 μm x 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm x 50 cm ...
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bioRxiv - Cell Biology 2022Quote: ... The growth medium was changed every 2 – 3 days and consisted of RPMI-1640 (for the RIN14B cells) (Fisher Scientific, 11-875-093) or otherwise DMEM/F12 (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were first trapped for 3 min on a C18-reverse phase trapping column (Acclaim PepMap® 100, 75 μm x 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), followed by separation on a C18-reverse phase analytical column (Acclaim PepMap® 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were pre-concentrated and desalted online using a PepMap C18 nano-trap column (length - 2 cm; inner diameter - 75 μm; particle size - 3 μm; pore size - 100 Å; Thermo Fisher Scientific) with a flow rate of 3 μL/min for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2022Quote: ... TFA as mobile phase on a trap column (Acclaim PepMap100, 2 cm length, 3 μm C18 particle size, 100 Å pore size, 75 μm inner diameter, Thermo Fisher Scientific) and finally separated at 60 °C using a 2 h gradient from 4 to 40 % (v/v ...
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bioRxiv - Immunology 2023Quote: ... The larvae were washed 2 additional times with 40 mls of sterile water followed by 3 washes with 40 mls of sterile PBS (Gibco™ PBS [10X]). Larvae were counted under a dissecting microscope using a McMaster slide and stored in vented U-shaped 275 ml tissue culture flasks (Polystyrene tissue culture flasks with vented caps ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Cell Biology 2023Quote: Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
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bioRxiv - Neuroscience 2023Quote: ... A full media change was performed every 2-3 days and cells were split by dissociation with TrypLE Express Enzyme (Gibco, cat no: 12605010).
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bioRxiv - Bioengineering 2023Quote: ... The plates were blocked by 3% BSA/PBS for 2 h and then incubated with serial dilutions of an EPO standard (Thermo Fisher Scientific, USA)) or culture supernatant samples for 4 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated and washed for 3 min on a C18 reverse phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6 μm particle size ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Neuroscience 2020Quote: Mice were physically restrained for 60 min in 50-ml polypropylene centrifuge tubes with 9 air holes of 3-mm diameter (339652, Thermo Fisher Scientific) (single exposure to restraint stress ...
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bioRxiv - Microbiology 2019Quote: ... JE-CVax hyper-immune and non-immune heat inactivated mouse serum samples (starting dilution of 1/9) were serially diluted 3-folds in RPMI 1640 medium (Gibco, ThermoFisher Scientific) supplemented with 4% low IgG serum ...
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bioRxiv - Microbiology 2020Quote: The concentration of culture supernatants and MMP-9 were measured by Human MMP-9 ELISA Kit (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... and propagated in medium further supplemented with Puromycin Dihydrochloride (Thermo Fisher Scientific) at a final concentration of 1 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Dihydrochloride (DAPI) and ProLong® Gold Antifade were purchased from Molecular Probes. Nitric Oxide Synthase activity assay kit was purchased from Abcam ...
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bioRxiv - Systems Biology 2023Quote: ... the cells were selected using 1 ug/ml of puromycin dihydrochloride (ThermoFisher), then grew until 80-90 % confluency ...
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bioRxiv - Immunology 2023Quote: ... and 2.5 µg/mL Puromycin Dihydrochloride (A1113802; GIBCO™, Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... and 2.5 µg/mL Puromycin Dihydrochloride (A1113802; GIBCO™, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Transduced cells were maintained in 2.5 µg/mL Puromycin dihydrochloride (Gibco, A1113803) selection media for 5 days before experimental use ...