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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated in freshly made serum-containing medium (GMEM supplemented with 10% foetal calf serum (FCS) batch-tested for ES cell culture (Gibco), 0.1 mM non-essential amino acids ...
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bioRxiv - Cell Biology 2024Quote: E14tg2a ESCs (RIKEN Cell Bank) were cultured in Glasgow minimum essential medium (GMEM; Wako) containing 10% fetal calf serum (FCS; Invitrogen), 1 mM glutamine (Wako) ...
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bioRxiv - Immunology 2024Quote: ... Tachyzoites were maintained in vitro by serial passages on confluent monolayers of HFF using DMEM supplemented with 1% FCS (GIBCO). For mouse infections ...
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bioRxiv - Immunology 2024Quote: ... Enzymes were quenched with PBS with 2mM EDTA and 2% FCS on ice and then red blood cells were lysed with room-temperature ACK lysis buffer (Gibco) and passed through a 100μm cell strainer.
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bioRxiv - Immunology 2024Quote: ... A single dose of 10 plaque forming unit (PFU) of PVM in 10 µl or the same volume of vehicle (10%, v/v, FCS in DMEM; Gibco) was administered via the intra-nasal (i.n. ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood as described for the PBMCs from the patients and cryopreserved at -150°C in fetal calf serum (FCS, Gibco) with 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... were cultured for 24h in 100 μL of RPMI medium containing 10% FCS in the presence of 5 μL of anti-CD3/CD28 beads (ThermoFisher). After centrifugation (450g ...
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bioRxiv - Microbiology 2023Quote: HeLa and HEK293T cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) with GlutaMAX and 10% decomplemented-FCS (fetal calf serum) (Gibco, Life Technologies). HeLa WT and HeLa BST2 KO cells were a kind gift of Stéphane Fremont (Institut Pasteur ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then washed and incubated with 1 μg/ml DAPI/20% FCS/0.3% TritonX-100/PBS and either anti-rabbit Alexa Fluor 488 (Invitrogen, A11070) and anti-mouse Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Freshly FACS-sorted cells were plated into 96 wells at 400,000 cells per well for each cell type and cultured for 24-48 hours in 200 ul of RPMI medium with 10% FCS (Gibco), 2 mM glutamine (ThermoFisher) ...
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bioRxiv - Physiology 2023Quote: ... and grown in DMEM/F12 with 10% FCS and penicillin–streptomycin (Life Technologies, final conc., 50 U/mL of penicillin). Differentiation was achieved by culturing the cells in differentiation media (DMEM/F12 with 2% horse serum and penicillin–streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 1:7500 goat anti-rabbit IgG A680nm and 1:7500 goat anti-mouse IgG A790nm for 1 hour at room temperature before being visualised on LiCor Odyssey Fc (Invitrogen).
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bioRxiv - Immunology 2023Quote: Surface antibody staining was conducted in PBS containing 0.1% FCS and 2mM EDTA for 30 minutes at 4°C to cells that had been pre-incubated with Fc block and LIVE/DEAD Blue (InVitrogen) staining ...
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bioRxiv - Immunology 2023Quote: ... and B16 were cultured inside tissue culture treated flasks with DMEM with 10% FCS (Hyclone) and 1X penicillin-streptomycin (100 units/ml, Gibco) at 37C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... All FCS measurements were carried out at ∼20 nM fluorescently labeled protein at 20°C in Nunc chambered coverslips (ThermoFisher). The chambers are pre-incubated with poly-lysine conjugated polyethylene glycol (PLL-PEG ...
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bioRxiv - Genetics 2023Quote: ... Cells were grown in DMEM with 10% FCS and penicillin–streptomycin (Life Technologies, final conc., 50 U/mL of penicillin). Differentiation into mature brown fat adipocytes was achieved by adding an adipogenic cocktail (0.5 μg/mL insulin ...
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bioRxiv - Developmental Biology 2023Quote: siRNA knockdown was performed as previously reported.20 9-12 week placental explants were placed into a 48-well plate with IMDM supplemented with 10% (v/v) FCS and gentamicin (50 μg/mL; Gibco). siRNA sequences targeting PRKCI (final concentration 0.2 μM ...
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bioRxiv - Immunology 2023Quote: Non-specific Fc binding was blocked for 10 minutes on ice with unconjugated anti-CD16/CD32 (93; Thermo-Fisher Scientific). Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were washed with PBS and resuspended in 200 µl buffer (PBS supplemented with 2% FCS) and analyzed via Attune NxT Flow Cytometer (ThermoFisher). For LIVE/DEAD analysis all cells were gated and viable cells were quantified as indicated in Figure 4 via FlowJo (Version 10.9.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with anti-CD16/32 (FC receptor block) at 1:100 and LIVE/DEAD Fixable (1:400-1:800; cat no., 15519340; Invitrogen) in 20 μl PBS for 10 min ...
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bioRxiv - Immunology 2023Quote: ... to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 4mM EDTA and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), CD4-AF488 (clone GK1.5 ...
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bioRxiv - Genetics 2023Quote: ... resuspended in 800 μL of PBS supplemented with 2 % FCS and analyzed on Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies rabbit anti-HA (1:500) was subsequently added in permeabilization/blocking buffer (10% fetal calf serum (FCS) (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... E14 mouse embryonic stem cells were cultured in plates coated with 1% gelatine in GMEM supplemented with 10% foetal calf serum (FCS) batch-tested for ES cell culture (Gibco), 0.1 mM non-essential amino acids ...
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bioRxiv - Physiology 2023Quote: ... nearly 106 cells were first incubated on ice for 30 minutes in a solution consisting of a 1:10 Fc Receptor Binding Inhibitor (eBiosciences) and 1:500 Live/Dead Aqua stain (Invitrogen) diluted in PBS ...
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bioRxiv - Immunology 2024Quote: ... and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS) and 100 U/ml of rhIL-2 (Gibco). 8×106 CTLs were transfected with 6 µg of plasmid DNA of each construct with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS; Capricorn Scientific, Ebsdorfergrund, Germany) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Dreireich, Germany) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen plugs were set at 37°C/5%CO2 for 10 minutes, and then fibroblast growth medium was added (DMEM medium supplemented with 10% FCS, 2 mM L-glutamine (ThermoFisher) and 100U/mL penicillin/streptomycin (ThermoFisher)) ...
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bioRxiv - Microbiology 2024Quote: ... and HKU1 NTD Fc + pA-LS at 50 μg/mL were incubated with StrepMAB-Classic (IBA) and goat αnti-mouse IgG HRP (Invitrogen) at a 4:2:1 molar ratio for 30 min on ice ...
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bioRxiv - Microbiology 2024Quote: The binding of KIRs-Fc fusion proteins to iRBCs was assessed by flow cytometric analysis with Attune NxT (ThermoFisher Scientific). Prior to the assay ...
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bioRxiv - Immunology 2024Quote: ... BHK21 cells were purchased from ECACC (85011433) and cultured in DMEM (supplemented with 10 % FCS, 10 mM HEPES (Gibco, 15630056), 1 mM Na-Pyruvate (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...
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bioRxiv - Synthetic Biology 2021Quote: ... supplemented with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with cells ...
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bioRxiv - Cell Biology 2020Quote: Human fibroblast cells lines were maintained in DMEM/F12 (Gibco) with 15% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng/ml recombinant human epidermal growth factor (Invitrogen, USA), 10 ng/ml human basic FGF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Human IL-6 and IL-8 ELISAs were from Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific, MA, USA), and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific, MA, USA), BV421-conjugated mouse anti-human CD34 (Biolegend ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney cells (HEK293) were maintained in IMDM (Gibco) supplemented with 10% FCS and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: Human PSCs resistant to neomycin were incubated with Accutase (Gibco) in 37°C for 5-10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Human staining panels included anti-CD3-Alexa 700 (Invitrogen, UCHT1), anti-CD19-APC (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... human recombinant Epidermal Growth Factor (5 ng/ml, ThermoFisher Scientific) and 0.15 mM CaCl₂ (Sigma-Aldrich) ...