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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... using a QuantStudio 3 PCR system (Thermo Fisher). Adipogenic genes tested included Peroxisome proliferator-activated receptor gamma (PPAR-G) ...
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bioRxiv - Immunology 2023Quote: ... then resuspended with 5 uM DiSBAC2(3) (Invitrogen), 2.5 uM DCFDA (AdipoGen Life Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... anti-V5 (Invitrogen, #R96025, 1:800, 3 days), anti-cFOS (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and an Applied Biosystems QuantStudio 3 (Applied Biosystems). Previously described primer sets (see Supplemental File 4 for sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 min incubation in 3% H2O2 (Fisher Scientific) was performed ...
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bioRxiv - Cell Biology 2023Quote: ... or 10µM TOTO-3 (Molecular Probes, Eugene, OR) in dPBS for 15 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... cells were loaded with Fluo-3-AM (Invitrogen) at 37°C for 30 min ...
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bioRxiv - Genetics 2024Quote: ... Cell number was determined by Countess 3 (Invitrogen) and was used to normalize oxygen consumption rate in pmoles/min.
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bioRxiv - Cell Biology 2024Quote: ... Amp-3 and HRP-tagged probe (ThermoFisher Inc) for 30 ...
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bioRxiv - Microbiology 2024Quote: ... 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; Invitrogen) while Ud23 and Ud26 IAV were labeled with a different lipophilic dye with a longer excitation and emission wavelength ...
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bioRxiv - Physiology 2024Quote: ... using the Quant Studio 3 system (Applied Biosystems). qPCR reactions were prepared in a final volume of 20 μl containing 2 μl cDNAs ...
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bioRxiv - Bioengineering 2024Quote: ... 4 × 10−3 M GlutaMAX supplement (35050061, Gibco), 2 × 10−4 M L-cystine (C7602 ...
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bioRxiv - Neuroscience 2024Quote: ... 3 g/L D-glucose (Thermo Fisher, A2494001), 0.5% NEAA (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... CD223 (LAG-3) (Invitrogen, eFluor 450, clone 3DS223H), TIGIT (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... and 3 mM L-glutamine (Gibco, 25030-024) or in phenol red-free DMEM (Gibco ...
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bioRxiv - Cell Biology 2024Quote: Pierce RNA 3’ End Desthiobiotinylation Kit (Thermo Scientific) was used for the biotinylation of the transcribed RNA for the pulldown experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated with 3% hydrogen peroxide (Thermo Fisher Scientific) in TBS and blocked with Avidin and Biotin (Vector Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3 µl of TURBO RNase (Thermo Fisher) were added and incubated at 37°C for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were stained in 3 μM DAPI (Invitrogen). Flow Cytometry was completed on BioRad ZE5 Cell Analyzer or BD Biosciences LSR15 cytometer ...
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bioRxiv - Physiology 2024Quote: ... and the QuantStudio 3 thermocycler (Thermofisher Cat. #A28567) using manufacturers instruction ...
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bioRxiv - Physiology 2024Quote: ... and the QuantStudio 3 thermocycler (Thermofisher Cat. #A28567) using manufacturers instruction ...
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bioRxiv - Neuroscience 2024Quote: ... 18 µl of 3 M sodium acetate (Ambion), and 2 µl of 10 mg/ml glycogen ...
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bioRxiv - Cell Biology 2024Quote: ... or a 3-8% Tris Acetate gel (Invitrogen). Proteins were wet transferred onto a PVDF membrane (Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... on the Qubit 3 Fluorometer (Q33216; Life Technologies). Total Rubisco content was measured by 14C-CABP binding as previously described (Ruuska et al. ...
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bioRxiv - Plant Biology 2024Quote: ... and Qubit 3 Fluorometer (Thermo Fisher, Waltham, USA), respectively.
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bioRxiv - Cancer Biology 2021Quote: The BCR-ABL-positive B-cell leukemia cell line BV173 (DSMZ, Braunschweig, Germany) was cultivated in RPMI1640 Supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco).
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bioRxiv - Cell Biology 2020Quote: ... the cell viability was determined using a micro plate reader (Thermo Scientific Multiskan FC, Thermo Fisher Scientific Oy, Ratstie, Finland) according to the manufacture’s protocol of CCK-8 ...
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bioRxiv - Cell Biology 2020Quote: HELA cells were plated in a 12-well plate in DMEM+ 10%FCS and transiently transfected using Lipofectamine3000 (Thermofisher, L3000001). Each transfection consisted of 250 ng expression plasmid (pcDNA3-control (Addgene ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Intestinal pieces were then placed into 10 mL RPMI 20% FCS with 0.5 mg/mL Collagenase Type I (Gibco #17100017) and 0.1 mg/mL DNase I (Sigma #DN25 ...
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bioRxiv - Microbiology 2022Quote: Unspecific Fc binding was blocked for 15 minutes on ice with unconjugated anti-CD16/CD32 (clone 93; Thermo-Fisher Scientific). Cells were subsequently fluorescently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using ThermoFisher LIVE/DEAD Fixable Aqua Dead Cell Stain and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Absolute cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Immunology 2019Quote: ... ATCC CRL-3216) cells were maintained in DMEM-Glutamax supplemented with 10% heat-inactivated fetal calf serum (FCS, ThermoFisher Scientific) and Penicillin-Streptomycin (PS ...
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bioRxiv - Immunology 2019Quote: ... whole stomachs were finely minced in disassociation buffer (2% FCS in Hank’s Balanced Salt Solution without calcium and magnesium (Life Technologies) and 5 mM EDTA ...
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bioRxiv - Developmental Biology 2019Quote: ... The remaining tissue was let to sediment and the supernatant was collected in a tube containing the same volume of 10% FCS (Gibco)-HBSS+/+ and kept on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... grown in DMEM supplemented with 10% FCS and neurospheres (RNS, enriched in cancer stem cells) grown in DMEM/Ham’s F12 GlutaMAX (Life Technologies) supplemented with B27 and N2 additives (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... cell number enumerated with a hemocytometer and resuspended in 0.5 ml PBS supplemented with fetal calf serum (FCS, ThermoFisher Scientific) to a final concentration of 1 × 103 cells/ml in each well of a 24 well plate ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured in DMEM (Biowhitaker) supplemented with 10% fetal calf serum (FCS) (v/v) and 1% penicillin/streptomycin (GIBCO). Inducible cell lines were selected on 5 μg/ml blasticidin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... were cultured and maintained at 37 °C in a humidified atmosphere of 5 % CO2 in air in Dulbecco’s Modified Eagle’s Medium (DMEM) + GlutMAXTM-1 containing 10 % Fetal calf serum (FCS) and 1% Penicillin-Streptomycin (10 000 U/ml) (Gibco Life Technologies ...
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bioRxiv - Immunology 2021Quote: Released tissue cells were stained in aliquots of 1 ×106 cells/100 μL of PBS with 2% fetal calf serum (FCS, GIBCO) and 0.1% NaN3 for 30min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS; Gibco). Harvested cells were washed with PBS ...
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bioRxiv - Bioengineering 2020Quote: ACE-Fc fusion proteins and the SARS-CoV-2 RBD were expressed in suspension-HEK293F cells grown in FreeStyle media (Gibco). Transfections were done using 40 kDa polyethyleneimine (PEI-MAX ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid coding for SARS-CoV-2 RBD or ACE2-Fc and were purified on Ni-NTA resin (Invitrogen) or Protein A resin (Cytiva) ...
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bioRxiv - Cancer Biology 2020Quote: ... BALB/c-derived A20 B lymphoma cell line was obtained from the American Type Culture Collection (Rockville, MD) and maintained in RPMI 1640 supplemented with 10% FCS (GIBCO). For in vivo experiments A20 cell lines were injected i.v ...
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bioRxiv - Cell Biology 2021Quote: ... washed twice with ice cold Cytoperm reagent and re-suspended in 1% fetal calf serum (FCS) in PBS (Invitrogen, 14190). Cells were subsequently analyzed on a CYAN flow cytometer (Beckmann Coulter).
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bioRxiv - Microbiology 2020Quote: ... Cells were maintained at 37°C in the presence of 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS) and 1% penicillin–streptomycin (fresh medium) (all from Invitrogen). Cells were used between 10 and 35 passages ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Neuroscience 2020Quote: ... After washing chase medium (DMEM containing 10% FCS, Glutamax, P/S, 1 mg/mL methionine and 1 mg/mL cysteine (Gibco)) was added to cells and incubated for different time points ...
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bioRxiv - Immunology 2021Quote: ... NK cells were then expanded for 6 days with hIL-15 50ng/ml (Miltenyi #130-095-765) in RPMI 20% FCS supplemented with L-glutamine (2 mM; Gibco), penicillin/streptomycin (100 μg/mL ...