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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... splenic NK cells were isolated and suspended in NK cell medium (phenol-red free RPMI 1640 containing 10% FCS, non-essential amino acids, L glutamine and sodium pyruvate, all from Gibco). The indicated target cells were labelled with 15μM Calcein-AM (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The overnight inoculum was diluted 1:33 in infection medium (DMEM + 10% FCS + HEPES (Life Technologies, cat. number: 15630-056)) to obtain OD600=1 after 3 h of incubation to give 1.5 × 109 bacteria/ml ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... University of Bristol) were grown in DMEM supplemented with 8% FCS and 8% (v/v) tryptose phosphate broth (Life technologies). Penicillin and streptomycin (90 IU/ml ...
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bioRxiv - Immunology 2022Quote: ... F-actin staining was performed at 4°C overnight in PBS 1x containing 0,1% TritonX100 2% FCS with Phalloidin AF405plus (Life technologies). Samples were washed in PBS 1x and mounted with Prolong Antifade Gold mounting medium (Life technologies) ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 medium supplemented with 10% heat inactivated FCS and final concentrations of 100 U/mL penicillin and 100 μg/mL steptomycin and 2mM L-Glutamine (Gibco). Cells were incubated with OVA peptide (323-339 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal calf serum (FCS) (Thermo Fisher Scientific, Hamburg, Germany), 100 IU/ml penicillin and 100 μg/ml streptomycin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Biopsies were washed in PBS and transferred into wells containing RPMI-1640 + 10% FCS + 20 μg/mL G418 (Thermo Fisher) + 20 U/mL Pen/Strep and cultured for 24 hours.
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Cancer Biology 2020Quote: ... The CH12.F3 mouse B lymphocyte cell line (a generous gift from T. Honjo (Kyoto Univ.)) was cultured in RPMI/10% FCS/5% NCTC-109 (Invitrogen)/1X Penn-Strep/50μM αMeOH ...
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bioRxiv - Microbiology 2020Quote: ... once and then 100 μl of 1:300 diluted secondary antibody (ThermoFisher Cat # A-21091 for Fc-tag and ThermoFisher Cat # MA1-21315-647 for polyhistidine-tag ...
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bioRxiv - Cancer Biology 2019Quote: ... All media were supplemented with 10% Foetal Calf Serum (FCS) (First Link, Birmingham, UK) as well as 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Blots were imaged using the LiCor Odyssey® Fc system after treatment with ECL Western Blotting-Substrat (Thermo Fisher, 32209).
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bioRxiv - Immunology 2022Quote: ... femurs and tibia of mice were flushed with complete medium (RPMI-1640 and 10% heat inactivated fetal calf serum (FCS) (Gibco). Single cell suspensions were incubated with 5 mL of red blood cell (RBC ...
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bioRxiv - Immunology 2022Quote: Surface antibody staining was performed in PBS containing 5% FCS and 1mM EDTA and using a Fixable Aqua Dead Cell (Invitrogen) to determine viability ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: 3T3-F442A preadipocytes were grown and differentiated in a modified DMEM designed for isotope labeling in cell culture [Sigma Aldrich (Saint Louis, MO, USA)] supplemented with 10% dialyzed FCS (GIBCO-Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... HT29 and LIM1215 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FCS (all from Life Technologies). Cells were maintained in a tissue culture incubator (Sanyo Europe ...
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bioRxiv - Cell Biology 2020Quote: ... Commercial colloidal force probes (CP-qp-CONT-BSA-A, NanoAndMore USA) were passivated with heat-inactivated fetal calf serum (FCS, Invitrogen) for 1h at room temperature to avoid non-specific adhesion of the bead to the cells ...
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bioRxiv - Pathology 2020Quote: ... cells were first synchronized overnight in 1% FCS DMEM and then cultured for 14 days in 10% FCS DMEM and stimulated daily with IL-1β (1 ng/ml; Invitrogen) or IL-17 at (2.5 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Immunology 2019Quote: ... The biopsy samples were collected in basic medium supplemented with 10% FCS and minced into pieces of 1 mm3 in PBS (Gibco). The biopsies were enzymatically digested with 1 mg/mL collagenase IV (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... 1% [v/v] FCS in PBS++) and primary antibodies were detected with secondary antibodies conjugated to Alexa Fluor dyes (Invitrogen). Images were acquired as above ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by addition of 0.5 N HCl and absorbance at 450 nm was measured using a Muktiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Neuroscience 2019Quote: ... with 10% fetal calf serum (FCS) at 37°C in an atmosphere with 5% CO2 and transfected with lipofectamine (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Fc Block was diluted in deficient RPMI (dRPMI; RPMI-1640 supplemented with HEPES and 1g/L sodium azide [ThermoFisher Scientific]) with 10% heat-inactivated FBS (dRPMI+FBS) ...
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bioRxiv - Immunology 2020Quote: ... gradient centrifugation and resuspended at 1-2 x 106 cells/ml in RPMI-10% FCS (RPMI 1640 w/L-glutamine (Cellgro Gibco) supplemented with 10% FCS (Cellgro Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in plating medium (Dulbecco’s modified Eagle’s medium (DMEM)/medium 199 [4:1 (v/v)]) containing 15% (v/v) foetal calf serum (FCS; Life Technologies) and 100 U/ml penicillin and streptomycin ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco, Waltham, Massachusetts) through the vena cava ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated for approximately 5 min before the reaction was stopped with the addition of 2 M H2SO4 and the A450 was measured (Multiskan FC, ThermoFisher). A standard curve was established using serial dilutions of lysate from PA11 ...
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bioRxiv - Immunology 2020Quote: ... washed 2x with PBS 0.5% FCS and then stained with the Click-it Plus OPP Protein Synthesis Kit Alexa Fluor 647 (C10458, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10 % foetal calf serum (FCS, Labtech) and 100 U/ml penicillin and 100 μg/ml streptomycin (Pen/Strep; Gibco). THP-1 cells were maintained in RPMI (Gibco ...
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bioRxiv - Immunology 2021Quote: ... cells from each vial were thawed in a water bath at 37°C and transferred to 10 mL thawing medium 10% FCS (Gibco) 1% Pen-Strep (Gibco ...
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bioRxiv - Immunology 2021Quote: ... 1.25 × 105 J76 cells were co-incubated with 2.5 × 105 K562 cells transgenic for HLA-A*02:01 in 96 well plates filled with 200 μL of IMDM media 10% FCS (Gibco) containing serial dilutions peptide of interest in three independent replicates ...
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bioRxiv - Immunology 2022Quote: ... from healthy donors were isolated from whole blood by density centrifugation on Lymphoprep (Axis-Shield PoC) and cryopreserved at −150 °C in FCS (Gibco) + 10% DMSO.
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in complete B cell medium (RPMI supplemented with 10%FCS, 25 mM Hepes, Glutamax, penicilin streptomycin (Invitrogen) and 1‰ß-mercaptoethanol (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Stop reaction was added in each well and protein was measured at 450 nm in a Multiskan FC (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... UM-SCC-47 cell line was cultured in three parts of Hams F-12 nutrient mixture medium and one part of DMEM with 10% FCS (Gibco) and 100 U/mL penicillin–streptomycin ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Content of two ampules containing the cell culture was resuspended in Hank’s solution supplemented with 2% FCS (fetal calf serum) (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Hepatocytes were then maintained for up to 1 week in hepatocyte culture medium (L15 medium, 8.4% FCS + 1% glutamine + 10% tryptose phosphate, Life Technologies) containing 1 μM insulin ...
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bioRxiv - Cell Biology 2022Quote: ... After two washes of the tissue with plating medium (DMEM containing 10% FCS, 2 mM glutamine, 50 mg/ml penicillin/streptomycin, Invitrogen), it was triturated in plating medium and subsequently cells counted ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed thrice with PBS and Minimal Essential Medium α containing additional nucleosides and 10% FCS (Thermo Fisher) was added ...
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bioRxiv - Microbiology 2022Quote: ... and the absorbance at a wavelength of 595 nm was measured using a plate reader (Multiskan FC, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The optical density was measured at wavelength 405 nm (OD405) by a microplate photometer (Multiskan™ FC, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: E14tg2a ESCs (RIKEN Cell Bank) were cultured in Glasgow minimum essential medium (GMEM; Wako) containing 10% fetal calf serum (FCS; Invitrogen), 1 mM glutamine (Wako) ...
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bioRxiv - Immunology 2024Quote: ... Tachyzoites were maintained in vitro by serial passages on confluent monolayers of HFF using DMEM supplemented with 1% FCS (GIBCO). For mouse infections ...