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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 2 to 4 µL was injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 µm C18 material (Thermo Fisher, 164564) using 0.1% formic acid in water (solvent A) ...
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bioRxiv - Biochemistry 2024Quote: ... After selection for 2 weeks in DMEM supplemented with hygromycin B (250 μg/ml; Carl Roth) and blasticidin (4 μg/ml; Thermo Fisher Scientific), single-cell colonies were isolated ...
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bioRxiv - Microbiology 2024Quote: ... The coupon was incubated in a 300 nM (100 ng/mL) solution of DAPI (4’,6-diamidino-2-phenylindole, ThermoFisher, Waltham, MA) for 3 minutes as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... CCL-2) or HEK-293T (ATCC CRL-3216) cells were seeded in 4-well Nunc Lab-Tek II chamber slide (Thermo Scientific, 154453) coated with Poly-L-Lysine (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS-T and counterstained with 0.2 μg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Cat# 62248) diluted in 1xPBS at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of cells were collected by centrifugation at 1500 rpm for 5 min at 4°C and stained with anti-SARS-CoV-2 RBD antibody (Invitrogen, PA5-114529). After centrifugation ...
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bioRxiv - Immunology 2024Quote: ... Cell concentrations were assessed by acquiring 30 μL of resuspended cells diluted 1:5 in PBS-4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) without any prior washing steps ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated for 5 min with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Dojindo, D523) and then mounted with PermaFluor (Thermo Fisher Scientific). Images were acquired using a BC43 or LSM800 instrument equipped with a Zeiss Axio Observer Z1 and a LSM 800 confocal unit with Airyscan module ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with the OPAL 780 fluorophore-conjugated anti-DIG antibody and 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, D1306, Thermo Fisher Scientific). Slides were then mounted using Prolong Glass Antifade mounting medium and left to cure overnight in RT ...
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bioRxiv - Biophysics 2024Quote: ... for 2 hours at 37°C (with 5% CO2 and humidity) before indicator loading with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies and reagents were used ...
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bioRxiv - Genomics 2024Quote: ... fluorescent dye DAPI (4′,6-diamidino-2-phenylindole) was added to count nuclei using a Countess II FL Automated Cell Counter (Life technologies, AMQAX1000). Bulk tagmentation was performed in a 50 μl reaction containing ...
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bioRxiv - Cancer Biology 2024Quote: ... on a rotating wheel for 2 hours at 4°C and 1 hour more incubation with 1.5mg Dynabeads protein G (Thermo Fisher Scientific, 10004A). IP fractions were washed four times with lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were stained with DAPI (4’,6-diamidino-2-phenylindole) by mounting the coverslips with Prolong-Gold Antifade containing DAPI (ThermoFisher Scientific, P36935). The slides were then analyzed using a Zeiss LSM 510 Meta confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... containing 4′,6-diamidino-2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies and reagents were used ...
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bioRxiv - Cell Biology 2024Quote: Cells (2 – 5 x 106) were washed two times with cold PBS and lysed with RIPA buffer at 4°C (Thermo Fisher Scientific) supplemented with protease inhibitor (Roche ...
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bioRxiv - Biophysics 2020Quote: ... hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine) (Invitrogen). Cleavage of PED-6 eliminates a self-quenching effect that results in the release of a BODIPY-FL dye ...
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bioRxiv - Developmental Biology 2020Quote: ... ES cells were passaged every 3–4 days using Accutase (Thermo Fisher) and used to passage 30 ...
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bioRxiv - Neuroscience 2020Quote: ... 1 nl solution of 3 μM FM 4-64 (Molecular Probes, Invitrogen) dissolved in DMSO was injected in the otic cavity of 96 hpf zebrafish embryos mounted laterally in low gelling agarose (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 nl solution of 3 μM FM 4-64 (Molecular Probes, Invitrogen) dissolved in DMSO was injected in the otic cavity of 96 hpf zebrafish embryos mounted laterally in low gelling agarose (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... blotted for 3–4 s in a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were grown for 3-4 days in Neurobasal medium (Thermo Fisher) supplemented with 2% FBS (HyClone) ...
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bioRxiv - Synthetic Biology 2021Quote: ... samples were diluted 3:1 in 4× NuPAGE LDS sample buffer (Invitrogen) supplemented with 10% beta-mercaptoethanol (v/v) ...
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bioRxiv - Microbiology 2020Quote: ... at 4 °C for 3 h on a Labquake rotator (ThermoFisher Scientific). The beads were washed with 1 mL cold lysis/binding buffer four times at 300 g for 4 min at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... Stained cells were cytospun (Thermo Scientific Cytospin 4 1000rpm for 3 minutes) onto 1.5 thickness coverslips and mounted with ProLongTM Gold mounting medium (Molecular Probes #P36934 ...
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bioRxiv - Cell Biology 2022Quote: ... in 4:3:1 ratio into 293FT cells (Thermo Fisher Scientific, #R70007) with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC-neurons were incubated with 3 µM Fluo-4 (Life Technologies #F14201) at 37°C for 20 min and imaged directly following washout on an LSM Zeiss 880 inverted confocal microscope using a 63×oil 1.4 numerical aperture objective and a 488-nm argon ion laser ...
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bioRxiv - Neuroscience 2024Quote: ... Invitrogen) and DiD (1,1’- Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt; Invitrogen) crystals were inserted under a stereo fluorescence microscope (MZ10 F ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged enzymatically every 3-4 days using 0.25% Trypsin (Gibco) diluted 1:3 in 1X PBS (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µM 3-isobutyl-1-methylxanthine (Thermo Scientific, Cat # 28822-58-4), 1 µM dexamethasone (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged every 3 to 4 days using Accutase™ (Gibco) for up to 10 passages.
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bioRxiv - Cancer Biology 2023Quote: ... in a 4:3:1 mass ratio with Lipofectamine 2000 (Invitrogen # 11668019) in a 2:1 ratio of Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2024Quote: ... 3-4 or 5-6 and Phusion DNA polymerase (Thermo Fisher Scientific). The resulting PCR mix was DpnI digested and 4 μl of the mix was used for transformation of E ...
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bioRxiv - Developmental Biology 2020Quote: ... hTSC were cultured in pre-coated 6-well plates (1 µg/ml Col IV) with 2 ml of EVT differentiation medium (EVTM: DMEM/F12, 0.1 mM 2-mercaptoethanol (Gibco 31350)) ...
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bioRxiv - Immunology 2022Quote: ... ∼50% of total lung was placed in a bead homogenizer tube with 1 mL of PBS with 2% FBS and 2% antibiotics/antimycotics (Gibco) and stored at -80°C ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from HEK293T cell line 30 hours post transfection (HA-NRP1 and SARS-CoV-2-S1493-685 and or HA-NRP1-4Cys-4Ala and SARS-CoV-2-S1493-685) using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... B.1.617.2 Spikes were generated by overlapping PCR using a codon-optimized wild-type SARS-CoV-2 Spike gene (GeneArt, ThermoFisher) that was synthesized (Biobasic ...
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bioRxiv - Bioengineering 2021Quote: ... Silica gel was prepared by adding 2 g silica particles to 40 ml aqueous NaOH (0.01N; CAS 1310-73-2; Fisher Scientific) solution and magnetically stirring the mixture at a speed of 1000 rpm for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The gland/crypt-containing supernatant was washed by centrifugation at 400 x g for 2 minutes and the glands/crypts re-suspended in 1-2 ml advanced DMEM/F12 (12634-010; Gibco) containing 1X B27 supplement minus vitamin A (12587-010 ...
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bioRxiv - Cancer Biology 2021Quote: ... were cultured in EBM-2 Basal Medium supplemented with EGM-2 MV Microvascular Endothelial Cell Growth Medium and 1% v/v Pen-Strep (ThermoFisher) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Staining the cells with the mitochondrial selective dye Rhod-2 AM (rhodamine-2 acetoxymethyl ester; Thermo Fisher Scientific, Darmstadt, Germany), allows for specific evaluation of mitochondrial calcium accumulation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μL (200 pmol) each of gRNA and tracRNA were combined with 2 μL of 5X annealing buffer (ThermoFisher 100061876) and 4 μL nuclease-free dH2O ...
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bioRxiv - Microbiology 2021Quote: ... coated with 200 ng/well ACE2 [produced in-house as described in Bosnjak et al.(25)] and blocked with 2% bovine serum albumin/2% mouse serum (Invitrogen) and incubated for additional 1 h at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of hK14-LaNto31-T2A-mCherry or LaNt-α31-pSec-Tag and 2 μl Lipofectamine 2000 (Thermo Fisher Scientific) were used ...
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bioRxiv - Bioengineering 2022Quote: ... Porcine DFAT adipocytes were stained for lipids via a 15-30 minute incubation at 37°C in lipid accumulation media (sans Intralipid) supplemented with 2 μM BODIPY 493/503 and 2 μg/ml Hoescht 33342 (H3570; Invitrogen). DFAT cells were then fixed in 4% PFA at RT for 10-15 minutes ...
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bioRxiv - Microbiology 2022Quote: The monoclonal antibodies SARS-CoV-1/SARS-CoV-2 Spike Protein S2 (1A9) and SARS-CoV-1/SARS-CoV-2 Nucleocapsid (6H3) were purchased from ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 2 µg fresh bacmid DNA was transfected into 2 mL Sf9 cells at 0.9×106 cells/2 mL with 8 µL Cellfection II (Thermo Fisher) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1% Glutamax (35050-038)) which was exchanged for 2 ml feeding medium 2 hours after plating (Neurobasal medium (21103-049, Gibco), 2% B27 (17504-044 ...
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bioRxiv - Biophysics 2020Quote: ... The resulting peptic peptides were collected for three minutes on a trap column (2 mm x 2 cm) kept at 0.5 °C and filled with POROS 20 R2 material (Thermo Scientific). The trap column was then placed in line with an ACQUITY UPLC BEH C18 1.7 μm 1.0 x 100 mm column (Waters ...
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bioRxiv - Cancer Biology 2021Quote: ... NPCs were plated on Geltrex in a 6 well plate in StemPro NSC SFM supplemented with 20ng/ml of EGF and bFGF for 2 days before switching to astrocyte differentiation medium consisting of DMEM with 1% N-2 supplement (Gibco), 1% GlutaMAX-I supplement (Gibco ...