Labshake search
Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... and Tetramethylrhodamine methyl ester (TMRM, 0.1µM, Thermo Fisher I34361) for 30 minutes at 37°C and 5% CO2 ...
-
bioRxiv - Bioengineering 2022Quote: ... mitochondrial membrane potential (tetramethylrhodamine methyl ester, TMRM; ThermoFisher Scientific) and mitochondrial superoxide (MitoSOX Red ...
-
bioRxiv - Neuroscience 2023Quote: ... containing 80μL N-methyl-trimethylsilyl-trifluoroacetamide (MSTFA; ThermoFisher #TS48915) and gently vortexed followed by 30 min dry heat incubation at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... then loaded with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethylester (BCECF-AM, 1.6 μM, Life Technologies) for 30 min at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... and then blocked for 2 h with PBS-Tween (PBST) containing 2% (v/v) goat serum (Gibco, MD, USA). Three-fold serial dilutions of serum (starting at 1:50 ...
-
bioRxiv - Developmental Biology 2021Quote: ... was cultured at 2-8×105 cells/ml in Fischer’s medium with 20% horse serum and 2 mM L-glutamine (all Gibco).
-
bioRxiv - Molecular Biology 2020Quote: ... proteins were dialysed in PBS for 2 x 2 hours using Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher). Protein aliquots were frozen and stored at −80°C until use.
-
bioRxiv - Immunology 2022Quote: Purified IL-2 expanded MAIT cells (MAITIL-2) were stimulated for 18 hours with anti-CD3/CD28 Dynabeads (Thermofisher), IL-12 (50ng/ml ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fura-2-acetoxymethyl ester (Fura-2-AM) and Pluronic F-127 were purchased from Molecular Probes (Eugene, OR, USA) and dissolved in dimethylsulfoxide (DMSO) ...
-
bioRxiv - Cell Biology 2021Quote: 2 mg of 0-2 hr embryos were harvested and RNA was extracted with TRIzol Reagent (#15596026, ThermoFisher Scientific). The PAT test was conducted following the manufacturer’s protocol (ThermoFisher ...
-
bioRxiv - Plant Biology 2021Quote: ... SALK_015201 and irr-2 genomic DNA using gene specific primers (Supplemental Table 2) with Phusion polymerase (Thermo Fisher Scientific) and cleaned up using the Gene-JET PCR Purification Kit from Thermofisher (catalog number K0701) ...
-
bioRxiv - Microbiology 2021Quote: ... 3×105 HEK293T cells were transfected with a 2:1:2 mass ratio of psPAX.2:pMD2.G:pLentiCMV/TO PuroDEST pUL21 (815 ng total DNA) using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... embryos were collected and brains were removed in ice-cooled Ca+2/Mg+2-free Hank’s balanced salt solution (Gibco) with 10mM HEPES (Thermofisher) ...
-
bioRxiv - Immunology 2022Quote: ... GATA-2-expressing vector was constructed by cloning synthesized mouse GATA-2 sequences into eukaryotic expression vector pcDNA3.1 (+) (Invitrogen) with restriction enzymes of NheI and EcoRI (NEB) ...
-
bioRxiv - Neuroscience 2022Quote: ... both somal and axonal compartments were incubated with 2 μM of the calcium indicator dye Fura-2-AM (Invitrogen) in imaging buffer consisting of HBSS [Ca2+ and Mg2+-free] supplemented with 10 mM HEPES (ThermoFischer) ...
-
bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
-
bioRxiv - Microbiology 2021Quote: Vero cells were seeded at 1.0 × 104cells per well with Dulbecco’s modified Eagle’s medium (DMEM; Welgene) supplemented with 2% heat-inactivated fetal bovine serum (FBS) and 2% antibiotic-antimycotic solution (Gibco) in a black ...
-
bioRxiv - Neuroscience 2021Quote: ... the tissue was washed twice with 2 ml Neurobasal supplemented with 2% B27 and 0.5 mM L-glutamine (all from Gibco), and dissociated by 8–10 bouts of trituration ...
-
bioRxiv - Cell Biology 2021Quote: ... and 2+2 μg of plasmid DNA encoding the proteins were mixed in Opti-MEM™ (Thermo Fisher Scientific) and applied onto the cells in a single well ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... the cell pellets were incubated for an hour in 2 uM 2’,7’-dichlorodihydrogen-fluorescein diacetate (H2DCF-DA, Invitrogen) in phenol red free media (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... counter stained with 2 mg/mL Alexa Fluor® 594 F(ab’)2 goat anti-mouse IgG (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... the dorsal cord was washed twice with 2 ml Neurobasal supplemented with 2% B27 and 0.5 mM L-glutamine (all from Gibco) and dissociated into single cells by 8–10 bouts of trituration ...
-
bioRxiv - Microbiology 2020Quote: ... were seeded with 3.0 x 105 /ml Vero E6 cells in 0.5 ml volumes of 2 x MEM medium containing Glutamax (2 x MEM Gibco) + 10% FCS (Sigma, ...
-
The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... cultures were concentrated roughly four-fold by centrifugation (2 min 2000 xg) and 2 μl cells were placed on a slice of 1.5% UltraPure agarose (Invitrogen) dissolved in minimal media ...
-
bioRxiv - Neuroscience 2023Quote: ... the animal’s brain was extracted and the meninges removed in a Petri dish containing cooled HBSS +/+ (Hank’s Balanced Salt Solution with Ca 2+ and Mg 2+: Gibco) to maintain their metabolism ...
-
bioRxiv - Physiology 2022Quote: Constructs for F4-2_Fab (hereafter F4-2) and L6-F4-2 were cloned into the pcDNA3.1(+) mammalian expression vector (Thermo Fisher). The F4-2 heavy chain contains a 6xHis tag on its C-terminus ...
-
bioRxiv - Neuroscience 2023Quote: ... iPSCs-derived neuronal cells were incubated with the cell-permeant Fura-2 acetoxylmethylester (2 μM; Molecular probes, Life technologies) for 1 h at 37 °C in culture medium ...
-
bioRxiv - Neuroscience 2023Quote: ... iPSCs-derived neuronal cells were incubated with the cell-permeant Fura-2 acetoxylmethylester (2 μM; Molecular probes, Life technologies) for 1 h at 37 °C in culture medium ...
-
bioRxiv - Bioengineering 2023Quote: ... Interleukin-2 (IL-2; Clinigen, US-CA) was diluted in phosphate buffered saline (PBS; Thermo Fisher Scientific, US-CA) and added at a final concentration of 300 I.U./mL ...
-
bioRxiv - Cell Biology 2024Quote: ... as previously described.26 The platelet pellet was resuspended in HEPES-buffered Tyrodes buffer with BSA 0.35% (w/v) containing 2 µM fura-2-AM (Invitrogen), and incubated for 45 minutes in the dark ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CM for 48 hours before loading with 2 μM fura-2 acetoxymethyl ester (Molecular Probes) in calcium imaging buffer (CIB ...
-
bioRxiv - Physiology 2023Quote: ... Ca2+ imaging of mouse islets was performed with Fura-2 AM (Fura-2-acetoxymethyl ester) acquired from Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Microbiology 2023Quote: ... Two microliters of culture were spotted onto a 2 cm x 2 cm pad containing 1.5% UltraPure agarose (Invitrogen) + either BG-11 (for Se ...
-
bioRxiv - Biochemistry 2023Quote: ... onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5 sec blot time at 100 % humidity and 22°C ...
-
bioRxiv - Genomics 2023Quote: ... Cells were blocked for 1-2 hrs using 2% (v/v) Bovine Serum Albumin (BSA, Thermo Scientific, Cat # 37525) + 5% FBS in HBSS ...
-
bioRxiv - Pathology 2022Quote: ... as above and left to attach for 2 hours before adding bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrDU, Invitrogen B23151) to a final concentration of 75 µM and left to proliferate for 6 hours ...
-
bioRxiv - Neuroscience 2024Quote: ... TG cultures were incubated with the calcium-sensitive dye Fura-2 AM (2 µM; Molecular Probes, Carlsbad, CA, USA) in Hank’s modified buffer ...
-
bioRxiv - Molecular Biology 2024Quote: Vero E6 cells growing in P24 wells were infected with SARS-CoV-2 (Wuhan-1) at a moi = 2 pfu/cell in 0.5 mL OPTIMEM (Gibco) under biosafety level 3 conditions ...
-
bioRxiv - Immunology 2024Quote: ... the inoculum was replaced with 150 μL of overlay medium (1:1 mix of Leibovitz’s L-15 medium supplemented with 0.1% pen/strep, 2% TPB, 1× NEAA, 2× Antibiotic-Antimycotic [Life Technologies] ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... supplemented with Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2; Gibco™, US-referred to as HMGS2 media) or Gibco™ Human Melanocyte Growth Supplement (HMGS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Stimulated cells were incubated in media supplemented with 10 ng/mL of recombinant mouse IL-2 (rmIL-2) (ThermoFisher).
-
bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked with 1x PBST containing 2% goat serum and 2 mg/ml BSA (Thermo Fisher Scientific, 37525), then incubated with 1/5000 Anti-Digoxigenin-AP (Sigma ...
-
bioRxiv - Cancer Biology 2021Quote: ... Peptides were injected into a C-18 trap column (Acclaim PepMap100, 75 μm i. d. x 2 cm, C18, 3 μm, 100 Å, Thermo Fisher Scientific) and further separated on a C-18 analytical column (Acclaim PepMap100 ...
-
bioRxiv - Cell Biology 2020Quote: ... in PBS and incubated for 2-3 hr in secondary antibodies (conjugated to Alexa-488, Alexa-568, and Alexa 647, Life Technologies,1:500) at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... Rehydrated embryos were blocked with 10% BSA (in PBST) for 2-3 hours and incubated with anti-GFP antibody (1:1000; ThermoFisher Scientific, A-11120) overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Cells were incubated with 100 ng/ml LPS for 3 hr followed by incubation with 2 µM Mitosox red (Thermo Fisher Scientific, M36008) for 30min ...
-
bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells were seeded into 12-well plates and were transfected with 1.5 μg the RPL plasmid and 0.5 μg Cas13b gRNAs (NTC, U1-1, U1-2, U1-3) while ∼80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, Cat.# L3000015). For RIP experiments ...
-
bioRxiv - Molecular Biology 2021Quote: ... They were maintained on irradiated mouse embryonic fibroblast feeder cells (p2 DR4 expanded MEFs from WT-MRC Cambridge Stem Cell Institute) and were passaged every 2-3 days using TrypLE (Thermo Fisher Scientific, 12605028). ROCK inhibitor (10μM ...