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Citations for Thermo Fisher :
5401 - 5450 of 10000+ citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... an aliquot of each enriched sample was injected onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, 3 μm, 100 Å, Thermo Scientific) coupled to an analytical column (EASY-Spray PepMap column ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from 2×106 cells (3 biological replicates for wild-type and CNOT3-DM cells) using Trizol reagent (Thermo Fisher Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and loaded onto a reverse phase trap column (Acclaim PepMap 100, 75µm x 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, U.S.A.) using an Ultimate 3000 for 50 µL at a flow rate of 10 µL min-1 ...
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bioRxiv - Immunology 2021Quote: ... Peptides were initially trapped on a 2 cm long trap column (Acclaim PepMap100, 3 μm C18 particle size, 100 Å pore size, 75 μm inner diameter, Thermo Fisher Scientific) for 10 min at a flow rate of 6 μl/min with 0.1 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid ...
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bioRxiv - Plant Biology 2021Quote: ... Five microliters of peptide mixtures were loaded onto an Acclaim PepMap™ precolumn (75 μm × 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm × 50 cm ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated in blocking buffer with 8 ng/μL of custom labeled secondary antibodies or of commercial IgG-gam-F(ab’)2-Alexa Fluor 647 (DOL ~ 3) (Thermo Fisher, A-21237) for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were harvested and fixed with 3 % PFA for 15 minutes and then stained with 2 μM of CellTracker™ fluorescent probes (ThermoFisher Scientific #C34552). Z-stacks were taken using a Leica TCS SP5 Matrix confocal microscope with the 20X objective ...
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bioRxiv - Cell Biology 2020Quote: Fibroblasts were plated in 96-well plates (10,000 fibroblasts/well) for 3 days in 2% FBS in DMEM supplemented with ascorbic acid (50 μg/mL) (Fisher Scientific, Waltham, MA, USA). Fibroblasts and matrix were then fixed and immunostained as detailed above ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA oligonucleotides were obtained as pre-designed siRNAs as follows: MFF-sense strand: 5’-CGCUGACCUGGAACAAGGAdTdT-3’ for exon 2 30 (Ambion, Austin, TX, USA); DLP1-sense strand ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were loaded onto an Acclaim Pepmap 100 C18 trap column (75 μm x 2 cm nanoViper, 3 μm, 100 Å) (Thermo Fisher Scientific) at 2 μL/min ...
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bioRxiv - Neuroscience 2022Quote: ... free floating NAc sections were first washed (3 × 5 min) in 1x PBS containing 2% Triton X-100 (PBST) (Thermo Fisher, Waltham, MA). Sections were then blocked in 5% normal goat serum (NGS ...
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bioRxiv - Microbiology 2021Quote: ... The phospholipolytic activity was measured according to 83 using a synthetic fluorescent substrate 1-palmitoyl-2-(10-pyrenyldecanoyl)-sn-glycero-3-phosphocholine (Molecular Probes, The Netherlands) and a Hitachi F-4000 spectrofluorimeter.
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bioRxiv - Evolutionary Biology 2022Quote: ... through PCR amplification using pair of primers 2 and 3 (Table S1) with a 2X Phusion Flash PCR Master Mix (Thermo Scientific, Cat. # F548S). Gibson Assembly (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried peptides were resuspended with 0.1%FA and 500ng peptides were loaded on a HPLC-column (75-μm inner diameter, 2-cm length, 3-μm particle size, 100-Å pore size, Thermo Fisher Scientific) at a flow rate of 2 μL/min ...
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bioRxiv - Microbiology 2020Quote: ... The peptide samples were loaded (2 µl) onto a C18-reversed phase Acclaim PepMap 100 trap column (2 cm x 75 µm, 3 µm particles; Thermo Fisher Scientific, USA) with 30 µL of buffer A (2% v/v acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap column (75 μm inner diameter × 2 cm, 3 μm, 100 Å; Thermo Fisher Scientific) for 3.5 min at 5 μL/min with 2% acetonitrile (ACN) ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 3 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL min−1 for 3 min ...
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E-cadherin regulates the stability and transcriptional activity of β-catenin in embryonic stem cellsbioRxiv - Cell Biology 2021Quote: ... Cells were replated at a density of 2×103 cells/cm2 every 3 days after dissociation with 0.25% trypsin–EDTA (1X) (Gibco, cat no. 25200-056). Cells were grown in Knockout DMEM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... An adenylated adaptor was ligated to the 3’-end of the captured transcripts (Supplementary Table 3) by using a mixture of T4 RNA ligase and truncated T4 RNA ligase 2 (ThermoFisher Scientific and NEB), which served as a template for the reverse transcription primer (Supplementary Table 3) ...
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bioRxiv - Genetics 2021Quote: ... Five microliters of peptide mixture were loaded onto an Acclaim PepMap™ precolumn (75 μm x 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm x 50 cm ...
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bioRxiv - Molecular Biology 2022Quote: ... TFA as mobile phase on a trap column (Acclaim PepMap100, 2 cm length, 3 μm C18 particle size, 100 Å pore size, 75 μm inner diameter, Thermo Fisher Scientific) and finally separated at 60 °C using a 2 h gradient from 4 to 40 % (v/v ...
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bioRxiv - Immunology 2023Quote: ... The larvae were washed 2 additional times with 40 mls of sterile water followed by 3 washes with 40 mls of sterile PBS (Gibco™ PBS [10X]). Larvae were counted under a dissecting microscope using a McMaster slide and stored in vented U-shaped 275 ml tissue culture flasks (Polystyrene tissue culture flasks with vented caps ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Cell Biology 2023Quote: Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Neuroscience 2023Quote: ... An imaging window was constructed from three layers of microscope cover glass (1 x 5 mm, 2 x 3 mm diameter, Fisher Scientific, no. 1) joined with a UV-curable optical glue (NOR-61 ...
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bioRxiv - Neuroscience 2023Quote: ... A full media change was performed every 2-3 days and cells were split by dissociation with TrypLE Express Enzyme (Gibco, cat no: 12605010).
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bioRxiv - Biochemistry 2024Quote: ... The samples were pre-concentrated and desalted online using a PepMap C18 nano-trap column (length - 2 cm; inner diameter - 75 μm; particle size - 3 μm; pore size - 100 Å; Thermo Fisher Scientific) with a flow rate of 3 μL/min for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Cell Biology 2022Quote: ... The growth medium was changed every 2 – 3 days and consisted of RPMI-1640 (for the RIN14B cells) (Fisher Scientific, 11-875-093) or otherwise DMEM/F12 (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were first trapped for 3 min on a C18-reverse phase trapping column (Acclaim PepMap® 100, 75 μm x 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), followed by separation on a C18-reverse phase analytical column (Acclaim PepMap® 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The plates were blocked by 3% BSA/PBS for 2 h and then incubated with serial dilutions of an EPO standard (Thermo Fisher Scientific, USA)) or culture supernatant samples for 4 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the LV myocardium of 2-month-old male WT and Rbm20ΔRS mice (n = 3 per group) using TRIzol Reagent (Life Technologies, Waltham, MA, USA) and treated with DNase I (cat# D9905K ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated and washed for 3 min on a C18 reverse phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6 μm particle size ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Females were then primed with subcutaneous administration of estradiol benzoate (17-β-Estradiol-3-Benzoate, Fisher Scientific, 2 μg dissolved in sesame oil) for the 3 days preceding any days on which they were recorded.
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bioRxiv - Evolutionary Biology 2024Quote: ... or 150 heads or tails 3 hours post amputation (hpa) were transferred to a 2 mL tissue grinder (Fisher Scientific, 50-212-708) and washed with artificial seawater ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were loaded onto a pre-column (Acclaim PepMap 100, 75 µm x 2 cm, C18, 3 µm and 100 Å particles, Thermo Fisher Scientific) before being separated over a 25-cm IonOpticks Aurora Series column (IonOpticks) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were washed with PBST 3×2 minutes then counterstained with a fluorophore conjugated variation of wheat germ agglutin (Invitrogen; W11261; 10ug/mL), phalloidin (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: Samples were loaded onto Acclaim PepMap 100 C18 column (75 μm × 2 cm, 3 μm particle size and 100 Å pore size; #164946, Thermo Fisher Scientific) and separated using nano-capillary C18 column ...
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bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
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bioRxiv - Genetics 2024Quote: ... and BSA (3%) for 4 hours at 4°C after which streptavidin-agarose UltraLink Resin (Thermo Fisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Genetics 2021Quote: ... at 85 V for 2 hours 15 minutes at an ambient temperature of 4 °C in NuPAGE™ Transfer Buffer (Invitrogen, UK) containing 10% 2-propanol (ThermoFisher Scientific ...