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Citations for Thermo Fisher :
5451 - 5500 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... or GFP CDS fragments were ligated into the linearized pYES2 shuttle plasmid (Thermo Fisher Scientific) and verified by sequencing ...
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bioRxiv - Physiology 2020Quote: ... The CaMPARI gene was cloned into pDONR-221 plasmid using BP clonase (Invitrogen, 11789-020) to generate PME-CaMPARI ...
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bioRxiv - Molecular Biology 2020Quote: ... were transfected with 0.5 μg of plasmid DNA and 2 μl TurboFect (Thermo Fisher Scientific). Following 2 hours incubation ...
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bioRxiv - Molecular Biology 2020Quote: ... Small-scale isolation of plasmid DNA was done with GeneJET miniprep kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... Purified plasmid concentration was examined using a NanoDrop One UV-Vis spectrophotometer (Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2019Quote: All plasmids were generated by PCR with subsequent restriction digest and ligation into pcDNA3 (Invitrogen). Primers are listed in Supplementary Table 6.
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bioRxiv - Immunology 2021Quote: ... 0.5 μg plasmid DNA was diluted added to 25 μL Opti-MEM (Thermo Fisher Scientific) and mixed gently ...
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bioRxiv - Molecular Biology 2019Quote: ... Each dish was transfected with 4μg of plasmid DNA and 20μL of lipofectamine 2000 (Invitrogen). 24 hours later ...
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bioRxiv - Developmental Biology 2019Quote: ... All CDS were TOPO-cloned into the pENTR/D™ Directional TOPO® plasmid (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and cloned into pCR-TOPO plasmid using TOPO TA cloning kit for Subcloning (ThermoFisher Scientific). pCR-TOPO plasmids containing genomic DNA sequences were transformed into TOP10 competent cells and individual colonies were selected and sequenced.
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bioRxiv - Cell Biology 2019Quote: ... The CD44s plasmid or an empty pcDNA3.1 mammalian expression vector (Invitrogen/ThermoFisher Scientific, Carlsbad, CA) was amplified using One Shot™ Top10 chemically competent E ...
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bioRxiv - Cell Biology 2019Quote: ... The CD44s plasmid or an empty pcDNA3.1 mammalian expression vector (Invitrogen/ThermoFisher Scientific, Carlsbad, CA) was amplified using One Shot™ Top10 chemically competent E ...
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bioRxiv - Genetics 2020Quote: ... into the BbsI digested pSPgRNA plasmid and were transformed into DH5a competent cells (ThermoFisher #18265017). The pSPgRNA and the Cas9 producing plasmid pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Cell Biology 2020Quote: ... the plasmid was transfected in 6-well plates using lipofectamine 2000 protocol (Thermo Fisher Scientific). The transfected cells were selected using optimal concentrations of G418 antibiotic (G418 Sulfate ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 μg of purified plasmid DNA was mixed with 5 μl Lipofectamine 3000 (Thermo Fisher) and used for transfection according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with plasmids and siRNA oligonucleotides by using Lipofectamine 2000 (Thermo Fisher Scientific) and Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were transfected into nTERT cells in a 1:1 ratio with Lipofectamine 2000 (Invitrogen). For KO cells media was replaced 24 hours later with media containing 500 ng/μl puromycin and incubated for a further 72 h ...
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bioRxiv - Biochemistry 2021Quote: ... For Infusion HD-directed mutagenesis template plasmids were amplified with Phusion HF (F530S, Thermo Scientific) using mutagenic primers that also create homologous arms which ...
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bioRxiv - Cell Biology 2021Quote: ... 10 g/10 cm dish of plasmids was transfected using Lipofectamine 3000 (Invitrogen, Waltham, MA). Cells were cultured for 16–20 h after transfection (short culture ...
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bioRxiv - Developmental Biology 2021Quote: ... primary CM and H9c2 cells were transfected with plasmids at 100nM using Lipofectamine 300 (Invitrogen), 24h after plating and analyzed at 72h ...
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bioRxiv - Neuroscience 2021Quote: ... 400 ng of plasmids were transiently transfected into HEK293T cells using lipofectine 2000 (Life Technologies). GCaMP6 was expressed to monitor the Ca2+ response ...
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bioRxiv - Biophysics 2020Quote: ... The cDNA products were purified using the PureLink™ Quick Plasmid Miniprep Kit (Life Technologies) and sequences were verified by the Australian Genome Research Facility (Sydney ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transiently transfected with plasmid cDNAs or sgRNAs using Lipofectamine 2000 (Invitrogen, 11668-019) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and inserted into the lentiviral vector pLenti6/V5-DEST plasmid (Invitrogen, gift from Torsten Wittman). The lentiviral mCherry-H2B plasmid was a gift from Torsten Wittman (Pemble et al. ...
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bioRxiv - Genomics 2019Quote: ... and 1020 ng transfer plasmid using Lipofectamine® LTX with Plus™ Reagent (Life Technologies), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All cloning was done with TA-vectors and plasmids compatible with the Gateway system (Invitrogen). EAY1098 was transformed using the standard Lithium-acetate PEG transformation protocol with the following plasmid pairs ...
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bioRxiv - Microbiology 2020Quote: ... were co-transfected with psPAX2 and pMD2G plasmids into 293T cells by Lipofectamine 2000 (Invitrogen) according to the manufacture provided protocol ...
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bioRxiv - Microbiology 2019Quote: ... 293T cells were transfected with 0.5 μg of plasmid via Lipofectamine 2000 transfection reagent (Invitrogen) and virus containing supernatants were harvested 48 hours post transfection ...
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bioRxiv - Genomics 2020Quote: ... 30 ng (100 ng) sgRNA plasmid and 1 μL (1.5 μL) Lipofectamine 2000 (ThermoFisher Scientific) were added following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The HEK293T cells were transfected with the indicated plasmids using PEI or Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Expression plasmids were generated using Gateway cloning technology (Invitrogen, Thermo-Fisher Scientific, Waltham, MA, USA). A cDNA of the F ...
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bioRxiv - Microbiology 2021Quote: ... DENV RNA was transcribed in vitro from pACYC-DENV2-NGC plasmid by mMessage/mMachine (Ambion) kits and electroporated into BHK-21 cells ...
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bioRxiv - Immunology 2020Quote: ... 8 pDUAL plasmids (250 ng each with 4 μl Lipofectamine 2000 (Life Technologies, Loughborough, UK) containing sequences of a complete IAV genome were used to transfect HEK293T cells simultaneously ...
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bioRxiv - Immunology 2021Quote: ... ε-subunits and rapsyn-GFP were each cloned into pcDNA3.1-hygro plasmid vectors (Invitrogen, CA) and cDNA encoding human full-length MuSK was cloned into pIRES2-EGFP plasmid vector (Clontech) ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA (pVAX1 or pVAX1-SARS-CoV-2 Spike) was transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... digested plasmid DNA served as the input for the mMessage mMachine SP6 Transcription Kit (ThermoFisher). The resulting mRNA was then further purified using the Rneasy Mini Kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... The pBa-AnkB440R1145Q-Halo plasmid was generated via GeneArt™ site-directed mutagenesis (Life Technologies) using primers R1145Q_F (5’-CGCATCATCACCCAAGACTTCCCACAG-3’ ...
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bioRxiv - Developmental Biology 2020Quote: ... Cas9 mRNA was transcribed from linearized pCS2-Cas9 plasmid using the mMachine SP6 kit (Ambion). To generate mutants ...
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bioRxiv - Neuroscience 2021Quote: ... These plasmids were further co-transfected into pre-grown HEK-293 cells using lipofectamine (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and subsequent extraction of plasmid DNA was done using a MidiPrep kit (Thermo Fisher Scientific) and 50 mL cell suspension in Lysogeny broth (LB ...
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bioRxiv - Genomics 2021Quote: ... Transfection of all reporter plasmids constructs was performed using TurboFect (R0531, Thermo Scientific, Waltham, USA). Renilla Luciferase pRL-TK-Rluc (Promega ...
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bioRxiv - Genomics 2020Quote: The resulting plasmid DNA samples were assayed using a Qubit® Fluorometer 3 (ThermoFisher, USA) according to the protocol for the Qubit™ dsDNA HS Assay Kit (Q32851 ...
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bioRxiv - Cell Biology 2021Quote: ... The fragment was cloned into KpnI/ApaI digested a pcDNA5-FRT-TO plasmid (ThermoFisher Scientific) downstream of a doxycycline-inducible CMV promoter (unfused T-TALE) ...
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bioRxiv - Neuroscience 2021Quote: ... Sparse transfections of plasmid DNA were performed after DIV9-10 with Lipofectamine 2000 (Thermo Fisher). GFP-expressing pyramidal neurons were identified by their characteristic morphologies and used for imaging or recording after either 24 or 48 hrs ...
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bioRxiv - Cancer Biology 2021Quote: ... At the time of transfection 750ng of total plasmid was diluted into 100uL OptiMem (Invitrogen). For individual SR protein transfections this included 250 ng of pCI-neo-HA-SR with 500ng of pCI-neo-HA-empty vector ...
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bioRxiv - Cell Biology 2020Quote: V5-PAF1 plasmid was obtained by cloning PAF1 in Gateway pcDNA3.1/nV5-DEST vector (Invitrogen). The untagged CDK9 plasmid was obtained by cloning the cDNA of HA-CDK9 (gift from Andrew Rice ...
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bioRxiv - Developmental Biology 2022Quote: Two μg of sgRNA containing plasmid was incubated with 10 μL of Lipofectamine 2000 (Invitrogen) for 20 minutes and then added to high confluency P19 cells for 4 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... 1.6μg of each plasmid was diluted in a mix of 500μl of OptiMEM media (GIBCO) and 4μl of Lipofectamine 2000 (Thermoscientific ...
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bioRxiv - Immunology 2022Quote: ... The plasmids were transiently transfected into HCT116 cell line using Lipofectamine 3000 transfection kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Transfection of plasmid vectors to HEK293 cells was conducted using Lipofectamine 2000 (Thermo Fisher Scientific) and Thermo Fisher Scientific’s recommended protocols for transfection using Lipofectamine 2000 ...