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Citations for Thermo Fisher :
5601 - 5650 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA was amplified in Subcloning Efficiency DH5α competent cells (Invitrogen, Massachusetts, USA; # 18265-017) and extracted using QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... Both constructs were synthesized commercially and cloned into pcDNA3.4-TOPO plasmid (Life Technologies B.V. Europe) that included a Tyr-Pho signal peptide and an N-terminal hexahistidine (6xHis ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid transfections were performed using Lipofectamine™ LTX with Plus reagent™ (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... plasmids or synthetic DNA fragments was performed using Phusion High Fidelity Polymerase (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... 1.2 μg of CAG Transposase plasmid and 3 μl of Lipofectamine 2000 CD (ThermoFisher Scientific) in 90 μl of OptiPro was injected in the dorsal aorta of 2.5-day-old embryos ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting variant DBT’ was subcloned into the pDEST plasmid using the Gateway system (ThermoFisher). For mammalian expression ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were transfected with plasmids by Lipofectamine LTX reagent with Plus reagent (Thermo Fisher) and Lipofectamine 2000 transfection reagent (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with the indicated plasmids and siRNAs using lipofectamine 2000 (Thermo-Fisher Scientific). Transfection was done with 50 ng of total plasmid (50 ng when only one plasmid was transfected or two times 25 ng for conditions with two plasmids) ...
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bioRxiv - Biochemistry 2024Quote: ... The quality and concentration of plasmid DNA were measured by NanoDrop one spectrophotometer (Thermo Scientific). Plasmids with various mutations were generated by PCR methods using Q5® Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Biophysics 2024Quote: ... Solution A [200 µL of a mixture of plasmid and Opti-MEM (Thermo Fisher Scientific)] and solution B [200 µL mixture of PEI and Opti-MEM at a ratio of 1 ...
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bioRxiv - Cell Biology 2024Quote: ... 300ng of plasmid was transfected using Lipofectamine Stem Transfection Reagent (Thermo Fisher Scientific, Cat# STEM00001) in 150µl Opti MEM ...
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bioRxiv - Biochemistry 2024Quote: ... using 0.6 µg of each plasmid and 0.6 µl of Lipofectamine 2000 (Invitrogen, 11668-027). A transfection rate of 2-5% was seen the next day ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transiently transfected with purified plasmids using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: The following additional plasmids were used in this study: pMK-PLEKHG3 was generated by Invitrogen GeneArt Gene Synthesis (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... and 150 ng of the ADAR2 overexpression plasmids were co-transfected with Lipofectamine 3000 (Invitrogen). Luciferase assays and evaluations of mRNA levels were performed as described above after 40 hours of transfection.
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bioRxiv - Microbiology 2024Quote: ... equi were prepared using a recombinant plasmid from the TA cloning® kit (Invitrogen, USA). A 10-fold serial dilution of the plasmid (from an initial concentration of 0.5×108 copy number/μL ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmids were constructed using Gibson Assembly EX Cloning Kit or T4 DNA ligase (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were transiently transfected into stably Cas9 expressing target cells using RNAiMax (Invitrogen, product #13778075). GFP positive cells were sorted by flow cytometry and sequenced by Sanger sequencing using the Mix2Seq kit (Eurofins).
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bioRxiv - Systems Biology 2024Quote: ... and 10µL cells and plasmid mix were electroporated using a 10µL pipet tip (Invitrogen #MPK1025) with the following settings ...
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bioRxiv - Microbiology 2024Quote: ... using PCR products from other plasmids or synthesized DNA fragments (GeneArtTM StringTM fragments, ThermoFisher, USA). The final GD donor plasmid included homology arms for the UL37-38 region ...
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bioRxiv - Cancer Biology 2022Quote: ... the slices were incubated with a secondary goat-anti-mouse or rabbit Alexa-488 or Alexa 563 antibodies or goat-anti-mouse-HRP (Invitrogen, Life Technologies, Carlsbad, CA) for 2 h followed by DAPI cell nuclei staining or Hematoxylin staining ...
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bioRxiv - Genetics 2022Quote: ... incubated in secondary antibody (either Alexa Fluor 488 goat anti-mouse [Fisher Scientific, Cat #: A11029] or Alexa Fluor 555 donkey anti-mouse [Fisher Scientific, Cat #: A31570]) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... labeling and hybridization to a mouse Affymetrix Clariom™ D array previously known as GeneChip™ Mouse Transcriptome Array 1.0 (Affymetrix, Santa Clara, CA). Raw intensity data (.CEL files ...
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bioRxiv - Bioengineering 2022Quote: ... Fluorophore-conjugated secondary antibody staining (anti-rabbit AlexaFluor® 488, anti-mouse AlexaFluor® 594, anti-mouse AlexaFluor® 647; Molecular Probes; 1:800) was performed at room temperature for 1 h in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Staining for surface markers was performed by incubating the cells in single cell suspension with anti-mouse CD16/CD32 mouse Fc block (Thermo Fisher Scientific, RRID: AB_467135) and 10% rat-IgG in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in the secondary antibody (goat anti-mouse Alexa Fluor™ 546, Thermofisher A-21052 or goat anti-mouse Alexa Fluor™ 633, Thermofisher A-21052) at 1:500 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were treated with goat-anti-mouse IgG Alexa Fluor 488 and goat-anti-mouse IgG Alexa Fluor 555 for 1 hour (both from Thermo Fisher Scientific, MA, USA). The staining procedure was conducted using the Antifade Reagent with DAPI (Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... A secondary antibody was used in TBS-T with a dilution of 1:5,000 for Anti-Mouse (Thermo Fisher, Goat anti-Mouse IgG, #31430) and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG ...
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bioRxiv - Cell Biology 2023Quote: ... or detected with a monoclonal mouse antibody (SantaCruz, sc-245, 1:250) and visualised with a donkey anti-mouse Alexa Fluor 647 secondary antibody (Molecular Probes, A31571, 1:1000). SCC1/ RAD21 was detected with a monoclonal mouse antibody (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Iowa City, IA), mouse anti-Futsch (22C10 DSHB, Iowa City, IA) and mouse anti-GFP at 1:1000 (#A-11120, Thermo Fisher Scientific, Waltham, MA). Secondary antibodies used in this study were goat anti-mouse Alexa Fluor 488-conjugated IgG at 1:500 (#A-32723 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with Mouse-on-Mouse IgG Blocking Solution for 1 h at room temperature (1:30; Thermo Fisher Scientific, Catalog no. R37621). Sections were incubated overnight at 4°C with primary antibodies—rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... and the Alexa Fluor 488 goat anti-mouse IgG (Invitrogen) secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... followed by Alexa Flour 488 anti-mouse (Thermo Fisher, Z25002) secondary antibody for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 568 donkey anti-mouse IgG (Thermofisher A-10037), Alexa Fluor 568 donkey anti-mouse IgG1 (Thermofisher A-21124) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 488 donkey anti-mouse IgG (Thermofisher A-21202), Alexa Fluor 488 donkey anti-mouse IgG2a (Thermofisher A-21131) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 488 donkey anti-mouse IgG2a (Thermofisher A-21131), Alexa Fluor 568 donkey anti-rabbit IgG (Thermofisher A-10042) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 568 donkey anti-mouse IgG1 (Thermofisher A-21124), Alexa Fluor 647 donkey anti-rabbit IgG (Thermofisher A-31573) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 647 donkey anti-mouse IgG (Thermofisher A-31571). ALL Alexa fluor secondary antibodies were used at 1:1000.
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-KRT 18 (1:10, Invitrogen, Carlsbad, CA, USA); mouse anti-KRT 14 (1:10 ...
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bioRxiv - Cancer Biology 2021Quote: ... or anti-mouse-Alexa Fluor 594(1:2000; A11005, Invitrogen) and additionally incubated in WGA ...
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bioRxiv - Cancer Biology 2021Quote: ... and AlexaFluor 568 conjugated to donkey anti-mouse (Life Technologies). Actin was stained using Texas Red Phalloidin (Life Technologies).
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bioRxiv - Biochemistry 2020Quote: ... and goat anti-mouse Alexa Fluor 488 (Life Technologies, A11029), followed by three more washes with PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and AlexaFluor 488–conjugated anti-mouse (1:250, Molecular Probes) were used as secondary antibodies and incubated for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Alexa Fluor 488 goat anti-mouse IgG (Invitrogen, A-11029), Alexa Fluor 568 goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... and a goat anti-mouse Alexa Fluor 488 (A11029, Thermofisher).
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bioRxiv - Cancer Biology 2021Quote: ... Mouse anti-Alpha-Smooth Muscle Actin (ACTA2) (ThermoFisher; 1:100), Rabbit anti-PDGFR beta (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... along with 1 ng/mL of mouse IFNγ (ThermoFisher #PMC4031). After 72 hours ...
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bioRxiv - Microbiology 2020Quote: ... and Alexa 488 Goat anti-Mouse antibody (Life technologies A11029)) and DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... and Alexa Fluor 568-conjugated anti-mouse secondary antibodies (ThermoFisher) at room temperature for 1.5 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... or Mouse IgG1 Isotype Control (MOPC-21) (ThermoFisher #MA1-10407) were added to the co-culture where indicated.