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Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Myc-tagged LCK containing plasmid was generated using pENTR/D-TOPO cloning kit (Thermo Scientific) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... the cDNAs for LRRK2-WT and LRRK2-G2019S were isolated from plasmids (Invitrogen, California, USA) for subcloning into LexAop plasmids (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNAs were quantified using the NanoDrop ND-1000 UV-Vis spectrophotometer (Thermo-Fisher Scientific), after which the DNA was precipitated and subsequently dissolved in Opti-MEM™ Reduced Serum Medium (Thermo-Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and 5 µg Bmal1:luc or Per2:luc plasmid constructs using Lipofectamine3000 (Thermo Fisher Scientific). After 48 h incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... or 5ug of 14-3-3ζ expression plasmid DNA utilizing lipofectamine 2000 (Thermo Fisher Scientific) following manufacturers protocol ...
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bioRxiv - Immunology 2021Quote: DNA plasmids were transformed in Max Efficiency DH5α Competent cells (Thermo Fisher Scientific, Waltham, MA), propagated in Luria Broth ...
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bioRxiv - Developmental Biology 2019Quote: ... Sense and antisense probes were transcribed from linearized plasmid DNA using the MEGAshortscript T7 (Ambion) and mMessage mMachine SP6 (Ambion ...
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bioRxiv - Cell Biology 2019Quote: ... GSC#9 was transfected with 1 μg plasmid using a Neon Transfection System (Life Technologies). 24 hours later ...
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bioRxiv - Microbiology 2019Quote: The cleaned plasmid samples were sequenced with the SOLiD next-generation sequencing system (Life Technologies) and the relative abundance of each plasmid was determined as described previously27,51 ...
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bioRxiv - Molecular Biology 2020Quote: ... or 100 ng pBJ5-SERINC5-HA or empty pBJ5 plasmid and Opti-MEM media (Gibco). The transfection mix was then added to Lipofectamine 3000 diluted in Opti-MEM and incubated at room temperature for 15 minutes ...
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bioRxiv - Genetics 2019Quote: ... 4 μg of plasmid DNA was diluted in 400 µl of serum-free DMEM (Gibco). The transfection reagent was briefly vortexed ...
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bioRxiv - Biochemistry 2019Quote: ... These constructs were synthesised as plasmid DNA (Table S1) using GeneArtTM gene synthesis (ThermoFisher Scientific), with the sequence of each provided in supplementary material (Fig ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were transfected using Turbofect according to the instructions of the manufacturer (Thermo Fisher Scientific). Stable cell lines were obtained by FACS after two weeks of selection at 200µg/mL hygromycin (InVivoGen ...
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bioRxiv - Developmental Biology 2019Quote: ... and ligated into the TOPO Cloning site of the Pcr4-TOPO plasmid (Invitrogen Life Technologies). Insertions were verified by DNA sequencing ...
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bioRxiv - Developmental Biology 2019Quote: ... and ligated into the TOPO Cloning site of the Pcr4-TOPO plasmid (Invitrogen Life Technologies). Insertions were verified by DNA sequencing ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... The plasmid were linearized and in vitro transcription was performed with MEGAscript T7 kit (Ambion), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and transfected with the respective plasmids encoding uS12 and its variants using Lipofectamine 2000 (Invitrogen), according to the manufacturers’ protocol.
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of plasmids mix was transfect using lipofectamine 2000 transfection reagent (Thermo Fisher 11668027) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... We verified the presence of the plasmid via colony PCR (using DreamTaq polymerase from Thermofisher). 10 positive clones were grown overnight in liquid LB with antibiotic and mini-prepped to obtain the plasmid DNA ...
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bioRxiv - Genetics 2020Quote: All plasmid constructs were generated by gateway cloning using LR Clonase II Plus enzyme (Invitrogen). The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320 ...
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bioRxiv - Biophysics 2021Quote: ... 200-400 ng plasmid DNA and 0.6 µl transfection reagent in 20 µl OptiMem (Gibco) were used per well for microscopy experiments ...
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bioRxiv - Immunology 2021Quote: ... Plasmids used in this study were obtained as follows: pLEX.MSC was purchased from Thermo Scientific; psPAX2 and pVSV.G were a kind gift from Dr Luís Moita (Instituto Gulbenkian de Ciência ...
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bioRxiv - Immunology 2021Quote: ... paired IgH and IgL plasmids were transfected into 293T cells by lipofetamine 3000 (Thermo fisher). Supernatants from 293T cells were harvested 6 -7 days after transfection and screened in the ELISA for IgG expression ...
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bioRxiv - Genomics 2020Quote: ... The transfection (1.5 µg of each sgRNA plasmid) was performed using lipofectamine 3000 (ThermoFisher Scientific), according to the manufacture instructions ...
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bioRxiv - Microbiology 2021Quote: ... 900 μg of plasmid in 25 ml of Opti-MEM transfection medium (Life Technologies, CA) was mixed with 5 ml of PEI-MAX (1.0 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... crescentus ParB (WT/mutants) were amplified by PCR and Gibson assembled into plasmid pENTR (Invitrogen) so that parB is flanked by phage attachment sites attL1 and attL2 i.e ...
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bioRxiv - Neuroscience 2020Quote: ... the product of PCR amplification was subcloned in a pCR2.1 plasmid (TA cloning kit, Invitrogen). The DNA insert and the hCPEB3 IDR PCR product were digested with XhoI and BamHI restriction enzymes (NEB ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with 12 µg plasmid DNA using Lipofectamine 2000 (Invitrogen/Thermo Fisher), following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with 12 µg plasmid DNA using Lipofectamine 2000 (Invitrogen/Thermo Fisher), following manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The RPRM and negative control plasmids were transfected into cells using Lipofectamine™ 2000 (Invitrogen). The medium was replaced with fresh complete medium 6 h later ...
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bioRxiv - Biophysics 2021Quote: ... HA-p75K349A or HA-p75R384A plasmids using Neon™ transfection system (Thermo scientific, Cat: MPK10025) prior to plating ...
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bioRxiv - Bioengineering 2021Quote: ... The promoter region was cloned into pGlow TOPO TA expression plasmid (Invitrogen, catalog number: K483001) following the product’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... the GeneJET plasmid miniprep kit was used according to the manufacturer’s recommendations (Thermo Fisher Scientific). The introduction of the desired mutations was confirmed by sequencing (Macrogen Europe ...
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bioRxiv - Biochemistry 2020Quote: ... and 3 μg of a plasmid to 500 μL Opti-MEM™ (Thermo Scientific #31985062). The transfection mixture for HeLa cells instead consisted of 6 μL Lipofectamine™ LTX Reagent with 3 μL PLUS™ Reagent and 1.25 μg of transfer plasmid in 500 μL Opti-MEM™ (Thermo Scientific #31985062) ...
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bioRxiv - Cell Biology 2021Quote: Plasmid DNA listed in Table S2 was stored and propagated in Top10 Escherichia coli (Invitrogen−). For plasmid isolation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells with stable integration of the plasmids were selected using 500 μg/mL G418 (Gibco). Colonies from single cells were selected by visual inspection for even expression of fluorescently tagged proteins.
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bioRxiv - Cell Biology 2021Quote: ... Linearized plasmid DNA was used to synthesize mRNA using mMessage mMachine SP6 Transcription kit (Invitrogen). Synthesized mRNA was purified by LiCl precipitation ...
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bioRxiv - Cell Biology 2021Quote: ... plasmids were linearised at a NotI site and transcribed using the MEGAscript T7 kit (Ambion). I-PpoI mRNA was subsequently polyadenylated using a Poly(A ...
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bioRxiv - Microbiology 2020Quote: Transfection of A549 cells with siRNAs or plasmids was carried out using Lipofectamine 2000 (Invitrogen) lipofection reagent according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR fragment was cloned in the Zero Blunt™ TOPO™ plasmid (Thermofisher) and transformed in E ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid transfection was performed in Hepa1-6 cells using the Lipofectamine 2000 reagent (Life Technologies) according to the manufacturer’s specifications ...
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bioRxiv - Biophysics 2021Quote: ... The resulting plasmid library was used to transform DH10B electro-competent cells (Thermo Fisher Scientific). Transformed bacteria were plated on LB-agar plates and incubated overnight at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNAs were then extracted using the NucleoBond™ Xtra midi EF kit (Fisher Scientific), following the recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... The constructed plasmid was then transfected into A549 cells with Lipofectamine 3000 Reagent (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: Fragments of target genes plasmid backbone were amplified via PCR using Phusion polymerase (Thermo Scientific). PCR reactions were performed in 50 μL reaction mixtures containing 3% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... and transfection with plasmid was carried out using Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific, USA) according to manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... and transfection with plasmid was carried out using Lipofectamine 2000 (Invitrogen, Thermo Fisher Scientific, USA) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: Cells were transfected with MYC expression plasmids (Ricci et al., 2004) and vector pcDNA3 (Invitrogen) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... The library plasmid was amplified using ElectroMAX™ Stbl4™ Competent Cells (Invitrogen, cat.11635018). The number of bacterial colonies was kept >500 times the number of sgRNA sequences in the library to maintain representation ...
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bioRxiv - Cell Biology 2021Quote: ... they were transfected with 1 μg plasmid DNA and 2.5 μl LipofectamineTM 2000 (ThermoFisher #11668027) in a total of 1 ml Opti-MEMTM media (ThermoFisher #31985070) ...