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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Immunology 2023Quote: ... and the extraction was repeated with 50 % ACN / 5 % Formic Acid (HCOOH) (LC-MS Grade, #85178, Thermo Fisher Scientific). The solution was dried in vacuum centrifugation for 2 hours 30 minutes (maximum vacuum pressure up to 5.1 ...
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bioRxiv - Biochemistry 2022Quote: Collected cells were washed and resuspended in staining buffer (PBS 1X +1% BSA) with Fc Receptor Binding Inhibitor Antibody (Invitrogen, # 14-9161-73) for 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and the presence of FRα-binding peptide in the nuclear fraction was shown by western blot analysis using the antibody against FRα1-binding peptide (Thermo Fisher, ref: PA5-101588).
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bioRxiv - Cell Biology 2020Quote: ... Plasma membrane and Golgi apparatus of transfected cells were stained with 0.5μg/ml lectin-HPA conjugated to Alexa Fluor 647 in PBS supplemented with 1% BSA (Life Technologies) and washed twice with PBS prior imaging ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were incubated with peanut lectin (PNA) conjugated to Alexa Fluor 647 (L32460, Thermo Fisher Scientific, 1:2,000) and DAPI (340-07971 ...
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bioRxiv - Molecular Biology 2020Quote: ... tomato lectin was visualized with 2 μg/ml streptavidin conjugated to either Texas Red or Alexa Fluor 488 (ThermoFisher). Dye-conjugated secondary antibodies (Southern Biotechnology ...
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bioRxiv - Developmental Biology 2022Quote: ... Acrosomes were visualized using 0.5 µg ml-1 lectin peanut agglutinin (PNA) Alexa Fluor 488 conjugate (L21409, Life Technologies). Sections were mounted under Dako fluorescence mounting medium and glass coverslips (GM304 ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL of undiluted anti-V5 antibody (Thermo Fisher R960-25), 5 μl of undiluted anti-H4 antibody (Sigma 05-858) ...
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bioRxiv - Neuroscience 2019Quote: ... and probed using antibodies against tau (Tau-5, Thermo Fisher Scientific) or antibody specific to Serine 396 of tau (Phospho-Tau S396 ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies used in this study include: tau 5 (ThermoFisher, MA-12808), tau-E178 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: TAU-5 (#AHB0042, Thermo Scientific), AT8 (#MN1020 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies included anti-FOXM1 (5 µg per CHIP reaction, Invitrogen, 702664) and normal mouse IgG (1 µg per reaction) ...
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bioRxiv - Neuroscience 2019Quote: ... Primary polyclonal rabbit antibodies against claudin-5 (1:500; Life Technologies) and ZO-1 (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit-hosted antibodies [anti-Claudin-5 (1:100, 34-1600, Invitrogen), and anti-Iba-1 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... 5 mg antibody was reduced with Bond-Breaker TCEP solution (ThermoFisher) for 1 hour at 37°C then desalted with Zeba 7 kDa spin columns to remove TCEP ...
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bioRxiv - Immunology 2021Quote: ... Plasmids encoding Ig heavy and light chain pairs were co-transfected using Polyethylenimine into 293FreeStyle cells (Invitrogen). Supernatants were assayed for secreted rAb and for HA reactivity using the HA CBA described above ...
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bioRxiv - Microbiology 2019Quote: ... Gag was immunoprecipitated by incubating the cell lysates with HIV-Ig bound to DynabeadsTM Protein G (Invitrogen) for 1 hour on a rotating shaker at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were transfected with plasmids encoding Ig heavy and light chain pairs with ExpiFectamine (ThermoFisher Scientific). Monoclonal antibodies were purified from the cell supernatant using sepharose Protein A (Pierce).
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bioRxiv - Neuroscience 2021Quote: ... streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... and binding revealed using SuperSignal West Pico Plus reagent (ThermoFisher) was used as chemiluminescent substrate ...
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bioRxiv - Cell Biology 2022Quote: ... in 100 μl of 1X Annexin Binding Buffer (Thermo Fisher) for 15 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... High-binding 96-well plates (Nunc MaxisorpTM, Thermo Fisher Scientific) were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck) ...
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bioRxiv - Immunology 2021Quote: ... High-binding 96-well plates (Nunc MaxisorpTM, Thermo Fisher Scientific) were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck) ...
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bioRxiv - Microbiology 2020Quote: Binding assays were performed in 96-well plates (Nunc MaxiSorp) coated overnight at 4°C with 100 µL of 5 µM AsaA_NRR ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific binding was blocked with Mouse IgG Isotype Control (Invitrogen) for 25 minutes at 4°C ...
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Complement-associated loss of CA2 inhibitory synapses in the demyelinated hippocampus impairs memorybioRxiv - Neuroscience 2021Quote: ... Streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 96-well Corning Costar high binding plates (Thermo Fisher Scientific) were coated with SARS-CoV-2 Sike protein in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... and stored in lysis/binding buffer (Thermo Fisher Scientific, USA). The messenger RNA (mRNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... Nunc Maxisorp high-binding plates were purchased from Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: High-protein binding 96-well microplates (3855, ThermoFisher, MA, US) were coated overnight with 10 µg/ml of either porcine collagen II (20031 ...
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bioRxiv - Molecular Biology 2022Quote: ... Ligand binding SARM1 was incubated with DSBU crosslinker (Thermo Fisher) to a final concentration of 200 µM for 30 min at 25℃ ...
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bioRxiv - Physiology 2022Quote: ... and ionized calcium binding adaptor molecule 1 (IBA1; Invitrogen MA527726) were added at 1:1000 and incubated at 4°C overnight in a humidity chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were stained with anti-GFP antibody (5 μg/ml, Thermo Fisher, RFP Tag Monoclonal Antibody RF5R) and whole node confocal scans were used to quantify percent metastatic area of total node via image thresholding in ImageJ ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we first extracted total RNA from pools of tissue derived from ten like individuals using TRIzol Reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... it was necessary to use a fluorescent DNA quantification method with high sensitivity like the Qubit dsDNA HS kit (Invitrogen). Quality assessment ...
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bioRxiv - Biochemistry 2020Quote: ... Further sub-cloning of ZmGl2 and ZmGl2-like sequences for plant expression experiments were performed using LR Clonase II Enzyme Mix (Invitrogen), using pEarleyGate100 vector (Earley et al. ...
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bioRxiv - Neuroscience 2022Quote: ... transfected (psDsRed-2Mito) striatal-like neurons in coverslips were washed with Hanks’ Balanced Salt solution (HBSS) (#14025, Thermo Fisher Scientific). Mitochondrial movement videos were acquired on a Zeiss Cell Observer Spinning Disk System (Carl Zeiss Microscopy) ...
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bioRxiv - Microbiology 2022Quote: ... WTC hiPSCc were passaged as small clumps for maintenance or single cell-like suspension for cardiac differentiation using Versene (Gibco) and mTeSR Plus supplemented with 10 μM Y-27632 (Tocris) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSC-derived Brain Microvascular Endothelial-like cells were treated with 25 μg/mL fluorescent transferrin conjugate (Thermo Fisher Scientific) and incubated at 37oC for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... the cell instructive LEGO-like scaffolds were prepared by loading microgels supplemented with 10 µg/mL of rhVEGF165 (Invitrogen, ThermoFisher) and PDGF-BB (R&D Systems ...
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bioRxiv - Bioengineering 2020Quote: ... the cell instructive LEGO-like scaffolds were prepared by loading microgels supplemented with 10 µg/mL of rhVEGF165 (Invitrogen, ThermoFisher) and PDGF-BB (R&D Systems ...
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bioRxiv - Developmental Biology 2022Quote: ... Then the monolayers containing a mixture of mononucleated CTB-like cells and multinucleated STB cells were partially dissociated using StemPro Accutase (A1110501, Gibco) for 8-12 minutes ...
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bioRxiv - Microbiology 2022Quote: Murine macrophage-like RAW 264.7 cells and human A549 lung epithelial carcinoma cells were cultivated in RPMI 1640 medium (Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were chosen for this study due to the absence of endogenous ASIC-like currents [53] and were grown using standard procedures in the following medium: Ham’s F-12 Nutrient Mixture (Life Technologies), 10 % fetal bovine serum (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... ATCC CCL-2) cells and 3T3-L1 (murine embryo fibroblast-like cells, ATCC CL-173) were grown in DMEM (Gibco) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Plant Biology 2022Quote: ... Like samples were combined into a single tube then quantified by high sensitivity DNA Qubit (ThermoFisher scientific, Cat. No. Q32854).
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... For both fungi, first we obtained fresh blastospores (i.e., yeast-like single cells) suspended in Grace’s Insect Medium (Gibco, Thermo Fisher Scientific) supplemented with 2.5% FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™, Thermo Fisher Scientific ...