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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and donkey anti-goat antibody labeled with AlexaFluor555 (5 µg/mL; A21432, Invitrogen) in 40 µL PBS-T for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary AlexaFluor-conjugated antibodies (Life Technologies; 1:500 in 5% BSA in PBS) with Hoechst 33342 dye were incubated for 1 h at room temperature in the dark ...
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bioRxiv - Biophysics 2024Quote: ... Beads had previously been conjugated with 5 μL anti-GFP antibody (Invitrogen, A6455) for 2 hours at RT ...
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bioRxiv - Cell Biology 2023Quote: ... All antibodies were diluted in 5% BSA and 0.1% Tween-20 (Fisher Scientific). Primary antibodies were incubated at 4°C overnight and secondary antibodies (Li-Cor ...
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bioRxiv - Cell Biology 2023Quote: ... All antibodies were diluted in 5% BSA and 0.1% Tween-20 (Fisher Scientific). The following antibodies/dilutions were used ...
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bioRxiv - Plant Biology 2019Quote: To assess GUS expression driven by the maize ubiquitin promoter in transgenic barley transformed with the pBRACT214m-GUS construct we collected different tissues and stained these with 1mg/ml of X-gluc (5-bromo-4-chloro-3-indolyl-B-D-glucuronic acid, Thermo Scientific, USA) in X-gluc buffer (100mM sodium phosphate buffer pH 7.0 ...
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bioRxiv - Physiology 2019Quote: ... to obtain images and then stored at 4°C in 5% acetic acid and 10% glycerol solution in a heat-sealable roll stock pouches (Fisher Scientific, #0181226E). Proteins in gels used for Western Blotting were then transferred to polyvinylidene difluoride membranes (Whatman) ...
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bioRxiv - Neuroscience 2019Quote: ... essential medium non-essential amino acids solution (1/100) and 5% (v/v) heat inactivated fetal bovine serum (Invitrogen, Paisley, Scotland, UK) and were maintained at 37°C in an atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resuspended in 35μl of 1% formic acid and 15 μl were loaded onto a trap column (PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 nM of scrambled control or 5’-tRFCys antisense locked nucleic acid (LNA) oligos were transfected into cells with Lipofectamine 2000 (Thermo Fisher Scientific). Experiments were performed 24-48 hours post transfection ...
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bioRxiv - Immunology 2021Quote: ... peptides (1 μg) dissolved in 5% formic acid and analyzed by LC-MS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) equipped with a Dionex ultra-high-pressure liquid chromatography system (RSLCnano).
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bioRxiv - Physiology 2022Quote: ... were homogenized by ultrasonication (Q55 Sonicator, Qsonica Inc., USA) in 5 volumes of 1 M perchloric acid (PCA; A228 Fisher Scientific, USA). The samples were neutralized by adding KHCO3 ...
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bioRxiv - Cancer Biology 2023Quote: Dried pellets were resuspended in LC/MS grade water:methanol (95:5) (Optima, Fisher Chemical) with 0.1% formic acid (Thermo Fisher Scientific Cat. 28905). Untargeted metabolomics analyses were performed using a Q-TOF (Agilent ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed three times for 5 min with TBST and nuclei were stained with DAPI nuclei acid stain (ThermoFisher Scientific, D1306) for 30 minutes before a final round of three times 5 minute washes in TBS-T.
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bioRxiv - Molecular Biology 2023Quote: ... The 24 fractions were subsequently dried and the peptides re-dissolved in 5% formic acid and analyzed by nanoLC–MS/MS system Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific), equipped with an UltiMate 3000 RSLCnano Liquid Chromatography System ...
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bioRxiv - Cell Biology 2024Quote: ... 12 alternating fractions were resuspended in 2% formic acid/5% acetonitrile and subsequently injected for analysis on an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific). For the yeast cell PISA ...
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bioRxiv - Biophysics 2024Quote: Quantitative determination of intracellular pH (pHi) was performed using the cell-permeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid AM (Thermo Fisher) in live imaged N2a cells at 48 hours of differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resuspended in Mass Spectrometry buffer (5% Formic Acid and 5% Acetonitrile) and were analyzed on a Liquid Chromatography Orbitrap Fusion Lumos Tribrid Mass Spectrometer (#IQLAAEGAAPFADBMBHQ, Thermo Fisher Scientific) equipped with a nano-Acquity UPLC system and an in-house developed nano spray ionization source ...
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bioRxiv - Developmental Biology 2021Quote: ... treatment and binding to Dynabeads M-280 Streptavidin beads (Thermo Fisher Scientific), second-strand synthesis was performed with Klenow Exo-enzyme (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... Pierce™ high-binding 96 well plates (Thermo Scientific™, Cat. #15041) were coated with 5×107 CFU/well viable B ...
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bioRxiv - Immunology 2019Quote: ... Cells were sorted into 100 µl of lysis/binding buffer (Life Technologies) and stored at 80 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... 2.5×105 TSCs were seeded into a low binding T25 flask (Nunc, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... the lysate was incubated with Ni-NTA agarose resin for binding (Invitrogen), and later the resin was transferred to a Poly-Prep chromatography column (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... SYBR Fast green double stranded DNA binding dye (Cat. 4085612, Applied Biosystems) was used ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... non-specific binding sites were blocked with Superblock buffer (37515, ThermoFisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding was blocked with 10% fetal bovine serum (GIBCO, Australia), and the sections were incubated with the primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The second was the f-actin binding mushroom toxin phalloidin (Invitrogen A12380) conjugated to Alexa Fluor® 568 ...
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bioRxiv - Immunology 2021Quote: Maxisorp high protein binding 96 well ELISA plate (Nunc, Thermo Fisher Scientific.) was coated with 2μg/mL goat anti-human Fc antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Maxisorp high protein binding 96 well ELISA plate (Nunc, Thermo Fisher Scientific.) was coated with 2μg/mL goat anti-human Fc antibody (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Non-specific binding was blocked by incubation in FACS buffer (Life Technologies). The unbound antibodies were removed using 5 ml wash buffer ...
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bioRxiv - Immunology 2020Quote: ... and FcγR-binding was detected using streptavidin conjugated HRP (Thermo Fisher Scientific) at 1/10000 dilution for 1 h ...
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bioRxiv - Immunology 2020Quote: ... A high-binding plate (Corning Costar 96 well ½ area, #3690; Fisher Scientific) was coated with 1 µg/mL anti-IFNγ XMG1.2 ...
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bioRxiv - Immunology 2020Quote: ... A high-binding plate (Corning Costar 96 well ½ area, #3690; Fisher Scientific) was coated with 1 μg/mL anti-IFNγ XMG1.2 (Biolegend ...
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bioRxiv - Systems Biology 2023Quote: ... 1M NaCl) and subsequently binding to MyOne Streptavidin C1 Dynabeads (Invitrogen, #65001) at room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... Pierce™ high-binding 96 well plates (Thermo Scientific™, Cat. #15041) were coated with 5×107 CFU/well viable B ...
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bioRxiv - Cell Biology 2022Quote: ... non-specific protein binding was minimised by incubation with CAS-block (Invitrogen) for 30 minutes and sections were then incubated with primary antibodies diluted in DAKO Antibody Diluent overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... the binding mixture was subjected to streptavidin resin (Thermo Fisher Scientific, 20349) adsorption for 16 h at 4°C on a rotary mixer ...
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bioRxiv - Biochemistry 2023Quote: ... Binding was detected by 2 µg/ml anti-mouse AF 647 (Invitrogen). After each incubation step ...
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bioRxiv - Cancer Biology 2023Quote: ... SYBR Fast green double stranded DNA binding dye (Cat. 4085612, Applied Biosystems) was used ...
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bioRxiv - Cell Biology 2023Quote: ... and washed 3 times before resuspended in binding medium (RPMI-1640 (Gibco) with HEPES adjusted to 20 mM ...
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bioRxiv - Evolutionary Biology 2024Quote: Binding reactions were assembled as follows: MyOne Streptavidin T1 Dynabeads (Thermo Fisher) were first washed in new RBNS binding buffer (25 mM Tris ...
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bioRxiv - Developmental Biology 2021Quote: Seminiferous tubule stages were identified based on the morphological characteristics of the germ cell nuclei and acrosome staining with AlexaFlour488/568-conjugated lectin PNA (L21409/L32458, Thermo Fisher Scientific). The sections were observed using a BX53 (Olympus ...
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bioRxiv - Biochemistry 2020Quote: Sera samples were depleted of albumin prior to analysis by lectin blot using the CaptureSelect™ MultiSpecies Albumin Depletion Product (Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with the fluorescent lectin concanavalin A (ConA) (100 μg mL-1) conjugated to fluorescein isothiocyanate (FITC) (Invitrogen, United Kingdom) for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% BSA in TBS-T for 30min before incubated with specific primary antibodies and the corresponding secondary antibody (Invitrogen, #A21206 and #A10038 ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were blocked with 5% fat-free milk and thereafter probed with primary antibodies 6xHis Tag Monoclonal Antibody (Invitrogen) followed by incubation with horseradish peroxidase-labeled secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then washed PBS and then incubated for 1 h at room temperature with secondary antibody solution containing 5% goat serum in PBS and the appropriate secondary antibodies conjugated to Mouse-555 (Invitrogen, Waltham ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were washed with TBST 3x for 5 min and incubated in corresponding secondary antibody for 1 hour room temperature: goat anti-rabbit antibody (Invitrogen) or rabbit anti-mouse antibody (Invitrogen) ...