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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for L Leucine N T Boc H2O 13C6 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... HeLa-M and HeLa T-Rex-Flp-In were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Thermo Fisher Scientific) and U2OS cells (ATCC ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μg of each antibody was diluted in 30 μl PBS-T and then incubated with 5 μl protein A-coated magnetic beads (30 mg/ml, Dynabeads, 10002D, Invitrogen) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... for less than 10 minutes and resuspended in T-cell Media (RPMI [Fisher, #11875093], with 10% FBS [Fisher, SH30071], 1% L-Glutamine [Fisher, #25030081], 1% Sodium Pyruvate [Invitrogen, #11360070] ...
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bioRxiv - Physiology 2023Quote: ... the slides were washed three times in PBS-T and incubated with goat anti rabbit IgG (H+L) secondary antibody conjugated with Alexa Fluor 568(1:1000, Invitrogen) for 1h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... N,N’-Dicyclohexylcarbodiimide (DCC, 0.02 mol; Fisher Scientific) was dissolved in DCM with Norbornene-2-carboxylic acid (0.04 mol ...
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bioRxiv - Plant Biology 2021Quote: ... Chemicals and H2O used for sample processing were analytical grade (Fisher Scientific). The xylem sap samples were prepared for GC-MS as described by Rotsch et al ...
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bioRxiv - Genomics 2019Quote: ... 0.5 % SDS in DEPC H2O) and 100 µg Proteinase K (Invitrogen #25530049) for 30 minutes at 55°C followed by Isopropanol Precipitation using GlycoBlue (Invitrogen #AM9516 ...
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bioRxiv - Genomics 2019Quote: ... in DEPC H2O with 0.5 U/µl of SUPERase (AM2696, ThermoFisher Inc) for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.05 U/μl High fidelity enzyme mix and nuclease-free H2O (Ambion) to make up a reaction volume of 10 μl ...
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bioRxiv - Neuroscience 2023Quote: ... resuspended in 100 μl 0.1% formic acid in H2O (Thermo Fisher Scientific), and quantified using the Pierce Quantitative Colorimetric Peptide Assay kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified DNA was eluted in 50 µl of nuclease-free H2O (Ambion). A second cleanup of the libraries was performed using 1 volume of AMPure XB beads (Beckman) ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Genomic DNA was eluted and stored in nuclease-free H2O (Ambion AM9937).
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bioRxiv - Neuroscience 2023Quote: ... Mobile phase A was 0.1% formic acid in H2O (Thermo Fisher Scientific) and mobile phase B was 0.1% formic acid (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... pellets were dissolved in 20 μL of nuclease-free H2O (Ambion, AM9937) by pipetting up and down ...
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bioRxiv - Microbiology 2023Quote: ... the cornea containing molten scaffold medium was placed epithelial side down in a 6 well-plate containing 400 µl of Ethylene glycol-O,O’-bis(2-aminoethyl)-N,N,N’,N’-tetraacetic acid (EGTA) (Fisher scientific) and was kept in a CO2 (5% v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Live cells were imaged using an Olympus 40x UPlanFL N Oil NA1.3 objective in imaging medium (Leibovitz’s L-15 medium (Gibco™ 21083027) without phenol red ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed using 2x ChamQ Universal SYBR qPCR Master Mix (Vazyme, L/N TE342F9) with QuantStudio3 machine (Thermofisher). The cycle procedure was at 50.0°C for 2 min ...
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bioRxiv - Physiology 2022Quote: ... and clinical CRS-isolate strain P11006 (obtained from Drs. N. Cohen and L. Chandler, Philadelphia VA Medical Center) (72) were grown in LB media (Gibco). For anti-bacterial assays ...
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bioRxiv - Physiology 2023Quote: ... and clinical CRS-isolates (Drs. N. Cohen and L. Chandler, Philadelphia VA Medical Center [56]) were grown in Luria broth (Gibco). For anti-bacterial assays ...
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bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™) for 3 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP ...
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bioRxiv - Cell Biology 2021Quote: Cells were grown in labelling medium containing 0.1 mg/ml normal lysine or 0.1 mg/ml 13C6-lysine (heavy) lysine (Gibco) for 4 passages (~2 weeks) ...
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bioRxiv - Microbiology 2022Quote: ... The 15N and 14N ratios of N2 were detected by isotope ratio mass spectrometer (Delta V Advantage, ThermoFisher), and the δ15N values of the pending samples were calculated after comparing with the international standard (Atm-N2) ...
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bioRxiv - Biochemistry 2019Quote: ... the antibody-coupled beads were washed three times in 100 μl PBS-T before labelling the antibodies with a 150x molar excess of EZ-Link-NHS-PEG4-Biotin (21329, Thermo Scientific) dissolved in DMSO (276855 ...
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bioRxiv - Microbiology 2022Quote: ... plates were washed three times with PBS-T and incubated with horse radish peroxidase (HRP)-conjugated goat anti-mouse IgG (H+L) (Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.15% (w/v) N,N′-methylenebisacrylamide (Invitrogen: M7279) was frozen in aliquots and warmed to 4°C before use ...
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bioRxiv - Cancer Biology 2021Quote: T-PER (Invitrogen) lysis buffer + 1x HALT protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: Genotype information was available for 21,001 NTR participants from 6 different genotyping arrays (Affymetrix 6.0 [N = 8,640], Perlegen-Affymetrix [N = 1,238], Illumina Human Quad Bead 660 [N = 1,439], Affymetrix Axiom [N = 3,144] ...
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bioRxiv - Biochemistry 2022Quote: ... N-ethyl-N’-(dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... coverslips were washed in filtered H2O and assembled into Attofluor cell chambers (Invitrogen). Imaging was performed in STORM buffer - 10% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... acetonitrile (ACN) and water (H2O) were purchased from Fisher Scientific (Fairlawn, NJ, USA). LC-MS grade methanol (MeOH ...
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bioRxiv - Cancer Biology 2020Quote: ... mixed with 50 µL alkaline solution (28% NH3 in H2O, Thermo Fisher Scientific) and incubated for 15 min at room temperature for phosphopeptide elution ...
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bioRxiv - Cell Biology 2020Quote: ... 7) rinsed with Milli-Q H2O and mounted with ProLong Diamond (ThermoFisher Scientific) and cured for 24-48 hours at RT (6).
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bioRxiv - Neuroscience 2022Quote: ... Total volume was filled to 250μl using RNase-free H2O (Thermo Fisher Scientific) followed by the addition of 750μl Trizol LS (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 200 nM of the membrane permeability indicator Sytox Green solved in H2O (ThermoFisher) was added alongside the antimicrobial compound ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 μL ERCC spikes (AM4456740) (diluted 1:40,000 in UltraPure H2O (InVitrogen 10977023) containing carrier tRNA (AM7119 ...
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bioRxiv - Molecular Biology 2022Quote: ... gel was stained in H2O + 100 µL SYBR Green (Life technologies; cat: S7563) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated for 3 minutes in Hoechst nuclear stain (1:1000 in H2O, Thermofisher), and mounted with Vectashield (VectorLaboratories ...
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bioRxiv - Neuroscience 2023Quote: ... and all instruments were washed with DEPC-H2O and RNase Zap (AM9780, Invitrogen). And time spent for the whole tissue collection process was constrained within 10 min.
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bioRxiv - Biochemistry 2023Quote: ... and then 40 µl of 50% ACN/H2O in 0.1% TFA (Thermo Scientific). The peptides were lyophilized and reconstituted in degassed 5% formic acid (FA ...
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bioRxiv - Biochemistry 2023Quote: ... Mobile phase A consisted of LC/MS grade H2O (LS118-500, Fisher Scientific), mobile phase B consisted of 20% LC/MS grade and H2O and 80% LC/MS grade acetonitrile (LS122500 ...
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bioRxiv - Microbiology 2023Quote: ... The mobile phases used were solvent (A) H2O (LC/MS grade, Fisher Scientific) + 0.1% formic acid (FA ...
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bioRxiv - Bioengineering 2021Quote: ... and non-essential amino acids (10 mM, glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, L-serine; Gibco). The pre-incubated viruses were then applied to Vero-E6 cell monolayers ...
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bioRxiv - Cell Biology 2024Quote: ... Rab8b-specific SMARTpool siRNAs (L-012116; L-004374; L-026032; L-019045; L-003905; L-008744; Horizon Discovery) using Lipofectamine RNAiMAX reagent (Invitrogen). Media was refreshed 24 h post-transfection ...
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bioRxiv - Bioengineering 2021Quote: Ovaries from 6 to 8 day-old CBA × C57BL/6 (F1) mice were collected and transferred to Leibovitz L-15 media (P/N 11415, Gibco, USA). The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM ...
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bioRxiv - Neuroscience 2023Quote: ... 1X N-2 (Thermo Fisher Scientific, 17502-048], 1X B-27 [Thermo Fisher Scientific, 17504-044], 1mM L-Glutamine [Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... N (75 fmol) and L (25 fmol) proteins and the T7 RNA polymerase (50 fmol) using lipofectamine 3000 (Invitrogen™ L3000150) for 3 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: T cells were stimulated with Dynabeads T-Activator CD3/CD28 (Gibco), 200IU/mL of IL-2 (PeproTch ...
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bioRxiv - Immunology 2024Quote: ... Sorted T cells were stimulated with Human T-Activator Dynabeads (Thermofisher) in RPMI-1640 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... 0.05% 3-(N,N-dimethylmyristylammonio) propanesulfaonate Zwittergent detergent (Acros Organics) in 1% methanol ...