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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and mounted with Cytoseal XYL (Fisher Scientific, 22-050-262).
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bioRxiv - Microbiology 2021Quote: ... coli strains were grown for 24 h at 37°C in 10 mL DMEM-HEPES (Gibco), resuspended in 500µL HBSS (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... Media was replaced 24 hours post-transduction with 10 µg/mL puromycin (Thermo Fisher Scientific A1113803). Cell Titer Glo (Promega G7573 ...
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bioRxiv - Immunology 2024Quote: ... The cells were treated for 18-24 hours with 10 ng/mL IL-1β (Gibco, #PHC0815) and 5 ng/mL TNF (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Vero cells were transfected in 24-well plates with 10 pmol siRNA using Lipofectamine 3000 (Invitrogen). After 2 hours ...
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bioRxiv - Cell Biology 2020Quote: Guts (15–20) were quickly dissected in cold 1× PBS and transferred to TRIzol Reagent (#15596026, Invitrogen). RNA extractions were performed as described in the TRIzol Reagent protocol ...
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bioRxiv - Genomics 2021Quote: 20 ul of RNA prepared as described above was resolved on a 15% TBE-urea gel (Invitrogen). Custom 18-nt and 30-nt ladders were used to size select 19-29 nt long RNA ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrated RNA (10-15 µg per sample) was then size fractionated by electrophoresis using 15% polyacrylamide-urea gels (ThermoFisher Scientific, catalog # EC68852BOX) run at 200 volts in TBE (89 mM Tris-borate ...
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bioRxiv - Cell Biology 2024Quote: ... fixed in 4% PFA for 15 minutes at 4 °C and incubated in 1X InVitrogen eBioscience Cell Permeabilization buffer (InVitrogen 00-8333-56) for 15 minutes at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... 20 μL of protein solution was incubated with 4 μL of LDS sample buffer and incubated at 100°C for 5 mins before 10 μL was loaded on a 4-12% bis-tris gel (NuPAGE™, Thermo Fisher Scientific). The gel was either stained with Coomassie blue or transferred to a 0.22 μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... before cells were spun down at 200xg for 5 min and fixed in 4% PFA for 10 min followed by permeabilization in perm buffer (Invitrogen, #00-8333-56) with 10 μg/mL Hoechst (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (2-5 μg) was resolved on a 15 % TBE-Urea gel (Invitrogen EC6885BOX). RNA of size between 17-25 nt was excised from the gel and extracted in 0.3 M NaCl overnight at 25°C ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were rinsed 5 times in L-15 with Penicillin/Streptomycin (Life Technologies, 15140-122) and minced in growth media (DMEM Ham’s F-12 ...
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bioRxiv - Cancer Biology 2020Quote: ... Transduced cells were selected by addition of 5-15 ug/mL blasticidin (Life Technologies, Inc) two days post-transduction ...
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bioRxiv - Immunology 2020Quote: PBMCs were incubated overnight (37°C, 5% CO2) in X-vivo 15 media (Fisher Scientific) supplemented with 5% AB Serum (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... the EV-conditioned medium was collected in polypropylene centrifuge tubes and centrifuged at 2000 ×g for 22 min at 4°C to pellet down apoptotic bodies and cell debris using a Sorvall ST 8R centrifuge (ThermoFisher Scientific, Osterode am Harz, Germany). The supernatant was transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge equipped with a 50.2 Ti rotor (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... a total of 80 mL EV-conditioned medium was collected in polypropylene centrifuge tubes and initially centrifuged at 300 ×g for 11 minutes at 4°C to pellet down dead cells followed by subsequent centrifugation of the supernatant at 2000 ×g for 22 min at 4°C to pellet down apoptotic bodies and cell debris using a Sorvall ST 8R centrifuge (ThermoFisher Scientific, Osterode am Harz, Germany). The supernatant was then transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge fitted with a 50.2 Ti rotor (Beckman Coulter ...
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bioRxiv - Physiology 2023Quote: Isolated atrial myocytes were stained with 4 µM di-4-ANEPPS for 10 minutes (Invitrogen) for membrane visualisation[3 ...
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bioRxiv - Bioengineering 2021Quote: ... Fluo-4 AM (10 μg ml−1, Life technologies) was added to the cell culture media for 30 min at 37°C.Scaffolds were then transferred in Tyrode’s buffer and videos were recorded either with no stimulation or while pacing at frequency of 1 and 1.5 Hz using c-PACE EM pace (IONOPTIX) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in RIPA buffer and 20 micro-g of total protein was subjected to Western blot analysis using 4-20% gradient gel (Invitrogen). The primary antibodies used in this study are ...
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bioRxiv - Biophysics 2020Quote: ... the cell medium was changed to 4°C cold Leibovitz’s L-15 CO2 independent medium (Gibco). Prior to microinjection ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting sample was centrifuged at 10,000xg for 15 min at 4°C (Thermo Fisher Scientific), and the supernatant was collected for further analysis ...
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bioRxiv - Microbiology 2023Quote: ... 3% sucrose) diluted 1:4 in Leibovitz’s L-15 medium without phenol red (Gibco, Waltham, MA) and an adjusted osmolality of 340 mOsm using 1 M sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl of sample were loaded into a NuPAGE 4-12% gradient Bis-Tris gel (Invitrogen). Protein was transferred onto PVDF transfer membrane (0.45 μm pore size ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Lysates were separated by SDS-PAGE (4-15% Bis-tris gel; Life Technologies, San Diego, CA) with 1X MOPS buffer (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... washed once with cold acetone and then dissolved in Laemmli buffer (Tris 10 mM pH 7.5, EDTA 1 mM [Fluka, Buchs, Switzerland], β-mercaptoethanol 5%, SDS 5%, glycerol 10% [ThermoFisher Scientific]). Sonication and centrifugation were repeated as above to pellet and eliminate possibly remaining cell debris ...
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bioRxiv - Neuroscience 2024Quote: ... or onto Amersham Hybond 0.2 um PVDF membranes (Cytiva 10-6000-22) at 80V for 120 min in a solution of Tris-Glycine Buffer (Thermo Fisher Scientific 28363). Membranes were blocked for 1 hr at room temperature in gentle shaking with blocking buffer (0.1% Casein [BioRad 1610782] in Tris Buffered Saline [TBS]) ...
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bioRxiv - Microbiology 2021Quote: ... lysates were spun at 10,000 x g for 10 minutes at 4 °C prior to reaction with 4- acetamido-4’-maleimidyl-stilbene-2,2’-disulfonic acid (AMS) (ThermoFisher Scientific). AMS alkylation was performed by vortexing the lysates in 15 mM AMS ...
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bioRxiv - Cell Biology 2020Quote: ... and pulse-labelled with 20 µM 5-ethynyl deoxy-uridine (EdU; Invitrogen) and 1 µM FuDR (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... with 5–20% fetal bovine serum (Thermo Fisher Scientific Cat#12483–020). After five to seven days ...
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bioRxiv - Immunology 2021Quote: ... and 5 mM EDTA) containing 20 mg/mL Proteinase K (Invitrogen, #AM2548), incubated overnight at 55°C followed by gDNA extraction with phenol/chloroform/isoamyl alcohol extraction and ethanol participation ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µL of proteinase K (20 mg/mL, Thermo Fisher 25530049) were added and samples were incubated at 65°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: Doxycycline hydrochloride (Dox, 20 mg/ml; Fisher Scientific BP2653-5, Pittsburgh, PA) was dissolved in 3% sucrose and provided ad libitum for 8 days pre-injury and for 8-21 days starting 6 weeks post-injury ...
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bioRxiv - Neuroscience 2022Quote: Doxycycline hydrochloride (Dox, 20 mg/ml; Fisher Scientific BP2653-5, Pittsburgh, NH) was dissolved in 3% sucrose and provided ad libitum for 8 days pre-injury and 8-14 days post-injury ...
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bioRxiv - Immunology 2024Quote: ... and labelled with CellTrace Violet (5 μM, 20 min, 37 °C, Invitrogen). Labelled CD4+ T-cells were co-cultured (4 d ...
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bioRxiv - Cell Biology 2024Quote: ... 200 mg of 5-ethynyl-20-deoxyuridine (EdU; Thermo Fisher Scientific, #C10337) dissolved in PBS were injected intraperitoneally 2 to 3 hours before euthanasia ...
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bioRxiv - Cell Biology 2021Quote: ... and then seeded at 500 cells/cm2 and transfected for 24 hours with 20 nM of siRNAs resuspended in Opti-MEM (Gibco), DharmaFECT siRNA transfection reagent (Fisher scientific ...
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bioRxiv - Immunology 2020Quote: ... following manufacturer instructions in the presence of 20 ng/mL IL2 (Novartis, #709421) were starved for 24 hours in RPMI 1640 (Invitrogen) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... FITC-Bm-SA (FITC-labeled toad B. maxima serum albumin (24)) or 20 μg/mL Ovalbumin-DQ (ThermoFisher, Cat D12053) in the dark at 26°C for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... snap-frozen tissues and OCT-embedded tissues were thawed for 24 hours at -20°C in RNAlater-ICE solution (Invitrogen). RNA and protein were harvested from the same sample using a modification of the Nucleospin RNA/Protein kit (Machery-Nagel) ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS and A549 cells were transduced for 24 hours and selected after seven days in 20 μg/mL blasticidin (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... which was boiled in the SDS loading buffer with 5% β-mercaptoethanol (Cat. #60-24-2, Acros Organics). The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks ...
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bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... WT cells were stained directly in the 24 well plate with 5 ng/ml FM4-64 (Thermo Fisher) dye ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h or 72 h incubation at 37 °C and 5% CO2 in the incubator (Cytomat, ThermoFisher), 25 µl CellTiterGlo® (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was fixed for 15 minutes in 10% buffered formalin (Fisher Scientific, 23-245685) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 15 μg of protein sample were separated on a 10% Bis-Tris gels (Invitrogen) and transferred to the nitrocellulose membrane (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... Bacteriological-grade 10 cm plates were prepared by adding 15 ml 1x PBS (Invitrogen) to each plate and incubating them at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Larval brains (10–15) were transferred to a microtube containing 500μl TRIzol (Life Technologies), and vortexed immediately for 10-15secs ...