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Citations for Thermo Fisher :
5401 - 5450 of 6212 citations for MORF4L2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Extracellular fragment of human CD4 comprising domains 1-4 of human CD4 and a C-terminal His6-tag was expressed in Expi293 cells according to the manufactureŕs protocol (Thermo Fisher Scientific). Cell supernatant was harvested by centrifugation 4 days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... The iPSC-O cells were cultured on mouse embryonic fibroblasts (MEFs) and proliferated using a medium for human ES cells [KNOCKOUT DMEM (Gibco,10829), 15% CTS KnockOut SR XenoFree Medium (Gibco,14150) ...
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bioRxiv - Neuroscience 2021Quote: ... we re-suspended cells in PBS/ 0.2% human serum containing 2 µg/mL 7-aminoactinomycin D (7-AAD) (Invitrogen, Cat# A1310). We carried out isotopic controls with irrelevant mouse IgG1-APC ...
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bioRxiv - Cell Biology 2021Quote: Quantitation of mitochondrial size in mouse and human MSCs were done by staining with Mito-tracker Red (Invitrogen/Thermo Fisher Scientific) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Quantitation of mitochondrial size in mouse and human MSCs were done by staining with Mito-tracker Red (Invitrogen/Thermo Fisher Scientific) using manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
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bioRxiv - Microbiology 2020Quote: ... Human nasal fluid samples were sonicated on ice (5 seconds at amplitude of 8 um; Fisher Scientific Model 705 Sonic Dismembrator) to create homogenous samples ...
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bioRxiv - Microbiology 2021Quote: ... 1 x 106 TM were resuspended in 1 mL RPMI media and either left unstimulated or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Molecular Biology 2020Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher, Waltham, MA, USA; Cat#10566016) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: The concentrations of protein samples from human brain whole homogenate preparations were measured via the bicinchoninic acid (BCA) assay (Micro BCA protein assay kit, ThermoFisher Scientific). 20 μg of total protein were mixed 1:1 with Laemmli buffer and boiled at 95 °C for 10 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Neuroscience 2022Quote: ... UM was changed for 2 days to an endothelial cell medium supplemented with retinoic acid (EC+RA: human Endothelial-SFM (Life Technologies), 1% platelet-poor plasma derived bovine serum (Alfa Aesar) ...
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bioRxiv - Microbiology 2022Quote: ... An inoculum of 2.5×104 CFUs of bacteria in 25 uL was mixed with 75 μL human serum from healthy volunteers (Cat# BP2657100; Fisher Scientific). The mixture was incubated at 37 °C and aliquots were taken at baseline and 1h to calculate viable CFUs ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... of human CD58 (UniProt residues 29-–204 + AviTAG + 6xHis) and human CD86 (UniProt residues 24–238 + AviTAG + 6xHis) were produced in Freestyle 293F suspension cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Madin-Darby canine kidney (MDCK) and human embryonic kidney HEK293T (293T) cells were passaged and maintained in Minimum Essential Medium (MEM + GlutaMAX, ThermoFisher Scientific) with 8.3% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat antibody conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/500 in PBS ...
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bioRxiv - Systems Biology 2019Quote: ... The poly(A)-tailed RNA in human CCRF-SB cells was isolated from the total RNA using a Fasttrack MAG Maxi mRNA isolation kit (Life Technologies), whereas the poly(A)-tailed RNA in yeast cells and mouse tissues was isolated from the total RNA using a Dynabeads mRNA Purification kit (Life Technologies ...
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bioRxiv - Pathology 2019Quote: ... After loading 100 μl of the sample-specific PCR mixture into one sample port of the microfluidic cards (Human ABC Transporter Panel; Applied Biosystems), the cards were centrifuged twice for 1 min at 280g and sealed to prevent well-to-well contamination ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA libraries were amplified and barcoded using Ion AmpliSeq Transcriptome Human Gene Expression core panel and Ion Xpress Barcode Adapter (Thermofisher Scientific). The amplicons were quantified using Agilent High Sensitivity DNA kit before the samples were pooled in sets of eight ...
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bioRxiv - Immunology 2019Quote: ... or 1×105 (96-well plate) naïve CD8+ T-cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 beat to T-cell ratio and cultured in 1 ml (48WP ...
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bioRxiv - Genetics 2019Quote: ... Two gene expression profiles GSE35956 and GSE35959 were based on GPL570 (Affymetrix Human Genome U133 Plus2.0 Array, Affymetrix, SantaClara, CA, U.S.A). We performed robust multi-array average approach (Hochreiter et al ...
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bioRxiv - Immunology 2019Quote: ... Human T-cells isolated were also used to generate T-cells blasts using the Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (Life Technologies) as per company’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Invitrogen #A18805), goat anti-human IgM (Invitrogen #A18835) ...
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bioRxiv - Biochemistry 2019Quote: ... pcDNA3.1P-gp was constructed by the insertion of cDNA encoding human P-gp in pcDNA3.1 (Thermo Fisher Scientific, Austin, TX, USA). To engineer P-gp–Venus ...
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bioRxiv - Genetics 2021Quote: ... into the melanoma cell line UACC 903 and multiple human melanocyte cultures (c23, c197 and c87) using Lipofectamine 2000 reagent (Thermo Fisher) for UACC903 or electroporation with the Lonza Amaxa P2 kit and the CA-137 protocol on the 4D-Nucleofector system for primary melanocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... StrepII- and MBP-tagged human PUM1 and PUM2 RD and RBD constructs were expressed in E.coli BL21 (DE3) Star cells (Thermo Fisher Scientific). Cells were grown in LB overnight at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Both of the in vitro systems depend on the application of three-dimensional embryoid body cultures derived from either mouse embryonic stem cells (mESC, ES-D3, ATCC, CRL-1934) or human episomal induced pluripotent stem cells (hiPSC, Gibco, A18945). The teratogenicity determination of the mEST is based on the half maximal inhibition of differentiation into beating cardiomyocytes (ID50 ...
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bioRxiv - Immunology 2020Quote: Soluble extracellular domain (ECD) of human CD58 (UniProt residues 29–204 or 29–213) was produced either in Freestyle 293F suspension cells (Thermo Fisher) or adherent ...
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bioRxiv - Physiology 2020Quote: ... The cells were transiently transfected for 16-24 hours with either human or mouse orthologs of TRPA1 using Fugene 6 transfection reagents and Opti-MEM (Thermofisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Soluble extracellular domain (ECD) of human CD58 (UniProt residues 29–204 or 29–213) was produced either in Freestyle 293F suspension cells (Thermo Fisher) or adherent ...
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bioRxiv - Microbiology 2020Quote: ... LAM antigen was diluted to the desired concentration in control human serum in high-recovery glass vials (Thermo Scientific, Waltham, MA) C5000-995 and incubated overnight (18−24 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... The human laryngeal cancer cell line (HEp2) was purchased from ATCC (CCL-23) and cultured in DMEM/F12 medium (Gibco, 11320033) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... chamber slides were incubated with secondary antibody Alexa-Fluor 488 goat anti-human IgG (H+L) (Invitrogen #A-11013, Paisley, UK) diluted 1:500 and Hoechst 33342 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... ATCC) and human kidney COS-7 cells (CRL-1651, ATCC) were cultured in Dulbecco’s modified essential medium (DMEM, Gibco 11995-065) supplemented with 10% v/v heat-inactivated fetal bovine serum (Gibco 12483-020 ...
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bioRxiv - Bioengineering 2021Quote: DiPS 1016 SevA hiPSC line derived from human skin fibroblasts were seeded and kept in culture on Geltrex (1% Invitrogen, USA)-coated culture flasks using mTeSR (StemCell Technologies ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Cell Biology 2020Quote: C2C12 myoblasts were transfected with a plasmid containing the N-terminal 88 amino acids of human cyclin B1 fused to EGFP using Lipofectamine 3000 (Invitrogen, L3000001) with the P3000 reagent according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Cancer Biology 2019Quote: ... and labeled on ice with 1:250 dilution of secondary antibodies for binding (anti-mouse 488, ThermoFisher A11059 or anti-human 647, ThermoFisher A21445) and expression (anti-chicken 647 ...
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bioRxiv - Biochemistry 2020Quote: ... and human CMP-N-acetylneuraminate-poly-α-2,8-sialyltransferase (ST8SIA4) (NP_005659)were codon-optimized for human expression and synthesized (Geneart, Thermo Fisher Scientific). A cDNA encoding a soluble version of human NCAM ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: The coding region of each opsin transcript was amplified from eye cDNA and subcloned in a modified pFRT-TO expression vector cassette derived from pcDNA5 and containing the human cytomegalovirus (CMV) immediate early promoter (Invitrogen, USA). The expression plasmid was modified to include a C-terminal tag by the monoclonal antibody FLAG epitope sequence (DYKDDDDK) ...
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bioRxiv - Immunology 2020Quote: ... by negative selection magnetic bead sorting (Miltenyi) and activated in complete RPMI supplemented with 30 U/ml IL-2 with anti-human CD3/CD28 dynabeads (Thermo Fisher) at a 1:1 cell-to-bead ratio.
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bioRxiv - Molecular Biology 2019Quote: ... After 24h the cell suspensions were transferred into a new flask and PBMCs were stimulated by adding Dynabeads Human T-Activator CD3/CD28 (Life Technologies) in a 1:1 cell-to-bead ratio ...
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bioRxiv - Genetics 2019Quote: ... Read alignment to the human genome reference (hg19/GRCh37) and variant calling were performed with the Ion Torrent Suite Software v5.02 (Thermo Fisher Scientific).
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bioRxiv - Physiology 2019Quote: ... Approximately 20-40 mg of human ventricular myocardium was homogenized in 1 mL of Trizol (Life Technologies #15596-026, Carlsbad, CA) with a TissueLyser LT (Qiagen N.V ...
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bioRxiv - Genetics 2020Quote: ... Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies) followed by Lithium Chloride precipitation and quantitation using a NanoDrop (Thermo Fisher Scientific)