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Citations for Thermo Fisher :
5301 - 5350 of 6212 citations for MORF4L2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: The human monocyte cell line THP-1 was purchased from ATCC (TIB-202) and routinely cultured in RPMI 1640 medium (Life Technologies) containing 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2021Quote: ... were incubated with 2.105 primary human mast cells (cell line established from a different donor) with or without anti-CD3/CD28-coated beads (Dynabeads™, Gibco) at a T cell:bead ratio of 10:1 in RPMI 10% FCS supplemented with 20 ng.mL−1 SCF ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-human CD69 together with Live/Dear-near IR (1:1000 in PBS; LD-NIR; ThermoFisher Scientific; Cat no L10119) for exclusion of dead cells ...
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bioRxiv - Immunology 2021Quote: ... The remaining cells were enriched for CD8+ cells with Dynabeads™ Untouched™ Human CD8 T Cells Kit (Thermo Fisher #11348D) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... we transfected one 100 mm plate of DLD1 BRCA2-/- cells at 70% of confluence with 10 µg of a plasmid containing human EGFP-MBP-tagged BRCA2 cDNA (corresponding to accession number NM_000059) using TurboFect (Thermo Fisher Scientific), 48h post-transfection the cells were serial diluted and cultured in media containing 1 mg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: CNVs were detected using the Affymetrix Genome-Wide Human SNP Array 6.0 (Cat No. 901182, ThermoFisher Scientific Inc., Waltham, MA, USA) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2019Quote: ... Human IgG and IgM was quantified in blood serum (1:1000 dilution) and culture supernatants (DILUTION) using the Human IgG/IgM total ELISA Ready-SET-Go! Kits (Invitrogen/eBioscience). All ELISAs were performed as per manufacturer’s protocols ...
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... Cells were re-suspended at 100µL/well with 1:1000 diluent with anti-human IgG-Alexa 488 (#A28175, ThermoFisher, Waltham, MA). Plates were incubated for 1 hour at 4°C ...
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bioRxiv - Genomics 2019Quote: ... The digested tissue was mixed with 40 ml of CRB (1% human albumin (CSL Behring) in 40 ml of DPBS -/- (Gibco, #14190094)) and shaken vigorously to liberate the stromal cells ...
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bioRxiv - Immunology 2019Quote: ... MRC5 human fibroblast cell line and A375 human melanoma cell line are cultured at 37°C under 5% CO2 in DMEM medium (Life Technologies) containing 1% glutamine ...
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bioRxiv - Neuroscience 2019Quote: Aß1-40 and Aß1-42 concentrations from cleared supernatants of differentiated iPSCs were quantified by using the Amyloid beta 40/ 42 Human ELISA Kits (#KHB3441 and KHB3481; Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... gondii tachyzoites were cultured in human foreskin fibroblasts (HFF) and in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX (Invitrogen, California, United States) supplemented with 10% fetal bovine serum and maintained at 37°C with 5% CO2.
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bioRxiv - Systems Biology 2019Quote: DNA encoding the SNAP-Tag was PCR-amplified from the pSNAPf vector (NEBs, #N9183S) to be inserted in frame into the pT7CFE1-CHis plasmid provided in the human IVT kit (ThermoFisher, #88882). The Gateway cloning cassette was introduced immediately after the SNAP tag sequence using the Gateway vector conversion system (Invitrogen ...
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bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA, Thermo Fisher Scientific) sequences using RNAiMax lipofectamine transfection reagent (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... hiPSC-CMs were reprogrammed from human cardiac fibroblasts as described previously (28) and maintained on Matrigel Membrane Matrix (Thermo Fisher Scientific) in mTeSR™ medium (Stem Cell Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... Human ESCs (WA-01; National Institutes of Health registry #0043) were maintained in Essential 8 medium (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin-amphotericin B solution (Lonza ...
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bioRxiv - Immunology 2019Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely recon-stituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Biochemistry 2019Quote: ... DSF was performed on human BACE1 (1 μg) using 96-well qPCR plates (AB Biosystems, UK) with 20x Sypro Orange (Invitrogen, UK) in 50 mM sodium acetate ...
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bioRxiv - Biochemistry 2019Quote: The human embryonic kidney (HEK) 293FT cells a high transfection efficiency isolate of the HEK293T cells were purchased from ThermoFisher (# R70007), and used without further authentication ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc and DC-SIGN-Fc and then stained with Alexa Fluor® 488 goat anti-human IgG antibody (Life Technologies) diluted 1/200 in PBS + 1% FCS or BB buffer and incubated 30 min on ice ...
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bioRxiv - Molecular Biology 2019Quote: Human atherosclerotic plaque or mouse aortic root specimens were first cut into 8 μm sections using a freezing microtome (Thermo Scientific). For immunofluorescence detection to detect Tf ...
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bioRxiv - Biochemistry 2019Quote: Protein concentration in g ⋅ L−1 of human serum was calculated using PierceTM Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... were immortalized with human telomerase reverse transcriptase (hTERT) and cultured in complete AmnioMAX C-100 Basal Medium (ThermoFisher Scientific, Carlsbad, CA) supplemented with 15% AmnioMAX supplement (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and cultured in a 10 cm dish containing irradiated MEFs in human embryonic stem cell (huES) medium containing DMEM-F12 (Life Technologies), knockout 10% serum replacement (Life Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... isolated T-cells were cultured at 2×106/mL in R10 media (RPMI 10% heat inactivated FBS) in the presence of 20 μl/ml of Dynabeads Human T-Activator CD3/CD28 (from Life Technologies). These activated primary T cells were tested as for the MJ cells assays in 3 different formats ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... A de-epidermized human dermis was obtained by incubating the dermis for one month in PBS containing gentamicin (0.5 mg/ml; Life Technologies, Inc.) and antibiotic/antimycotic (Life Technologies ...
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bioRxiv - Biophysics 2019Quote: Human embryonic kidney cells 293 (HEK293-6E, NRC, Canada) were cultured in FreeStyle F17 expression medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 2 mM L-glutamine (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: HeLa human cervical cancer cells transfected in conjunction with fluorescence ubiquitination cell cycle indicator (FUCCI) cell cycle sensor (Life Technologies, P36237) were kindly donated by Dr ...
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bioRxiv - Cell Biology 2019Quote: The region from −2662 to −1 bp (relative to translation start site) of the human TMEM135 promoter was synthesized by Life Technologies. Additionally ...
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bioRxiv - Microbiology 2020Quote: The spike protein gene from MERS-CoV strain Camel/Qatar/2/2014 (GenBank accession number KJ650098.1) was codon optimized for humans and synthesized by GeneArt (Thermo Fisher Scientific). The synthesized S gene was cloned into a transgene expression plasmid comprising a modified human cytomegalovirus immediate early promoter (CMV promoter ...
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bioRxiv - Genetics 2021Quote: The human lung epithelial cell line Calu-3 (UC Berkeley Cell Culture Facility) was maintained RPMI media supplemented with Glutamax (Thermo Fisher), 10% FBS (HyClone) ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Genomics 2021Quote: Human embryonic kidney 293T (HEK293T) cells were cultured in DMEM media (TH.Geyer, L0102) supplemented with 10% FBS (Thermo Fisher, 10500-064) and 100 U/ml Penicillin and 100 μg/ml Streptomycin (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Immunology 2021Quote: ... with unconjugated rabbit anti-human phosphorylated NF-κB p65 (Clone: Ser536 93H1) followed by goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher). For p38 ...
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bioRxiv - Immunology 2021Quote: ... and either left them untreated (hence naive) or activated them ex vivo with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific), wherein the bead-to-cell ratio was 1:1 ...
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bioRxiv - Immunology 2021Quote: ... at a concentration of 1×105 cells/100μl of complete medium with or without Human T-Activator CD3/CD28 Dynabeads® (ThermoFisher Scientific). NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes ...
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bioRxiv - Microbiology 2021Quote: ... and the human microglial cell line HMC3 (ATCC® CRL-3304™) were cultured in Dulbecco’s Modified Eagle Medium (10566016, Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells (authenticated by ATCC through their human STR profiling cell authentication service) were maintained in high glucose DMEM with Glutamax (Gibco 31966021), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes 45 using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The raw spectra were searched against the Human (Homo sapiens) reference proteome database (UNIPROT) using SEQUEST HT (Proteome Discoverer v1.4, Thermo Fisher Scientific) with a Full MS precursor mass tolerance of 20 ppm and MS2 peptide fragment mass tolerance of 0.5 Da ...
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bioRxiv - Bioengineering 2020Quote: CD4+ T cells were electroporated prior to stimulation with anti-CD3/CD28 beads (Dynabeads Human T-Activator CD3/CD28, Thermo Fisher Scientific (ThermoFisher), Waltham ...
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bioRxiv - Bioengineering 2020Quote: Cytokine analysis was performed on supernatants derived from cultures given the indicated treatments using a human cytokine 10-plex panel (Thermo Scientific) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... human muscle biopsies from healthy donors were minced and digested in 0.8% w/v collagenase I (Life Technologies; Carlsbad, CA, USA) in DMEM ...
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bioRxiv - Neuroscience 2021Quote: The medial and baso-lateral amygdaloid nuclei were dissected from the mesial superior temporal lobe for 0.75-1 cm thick coronal slabs of frozen human brains on dry ice using a stainless steel punch (8mm diameter Thermo Fisher Scientific Integra Miltex standard biopsy punch) ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Neuroscience 2021Quote: ... 600 ng total RNA of human primary mesenchymal stem cells were reversely transcribed using the RevertAid RT Reverse Transcription Kit (K1691, Thermo Scientific) according to the manufacturer ...