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Citations for Thermo Fisher :
5351 - 5400 of 6212 citations for MORF4L2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and Human Embryonic Kidney 293 (HEK) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies- Invitrogen, Carlsbad, CA, USA). Both media were supplemented with 10% Fetal Bovine Serum Gold (FBS ...
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bioRxiv - Cell Biology 2021Quote: Human PCNT siRNA (Smart Pool) (M-012172-01-0005; Dharmacon) was transfected into cells with Lipofectamine RNAi MAX (13778100; ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Human Embryonic Kidney (HEK293T) cells were cultured in Dulbecco’s modified Eagle’s Medium–Glutamax medium (DMEM) (Gibco, Waltham, MA, USA, #6196-026) 10% FBS at 37°C and 5% CO2 with renewal of culture medium three times a week.
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid DNA construct was used to transfect Expi293F human cells using the Expi293F transfection kit and Expi293F expression medium (Thermo Fisher). Cells were harvested by centrifugation at 1,000 g for 30 min and resuspended in 1 x PBS supplemented with 30 mM imidazole ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Microbiology 2020Quote: ... A549 (human lung carcinoma) cells were used for infection experiments and were maintained in Ham’s F12 nutrient mixture (Thermo Fisher Scientific) with 8.3% FBS.
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bioRxiv - Microbiology 2020Quote: ... Goat anti-Rabbit IgG Alexa Fluor 647 and Goat anti-Human IgG Alexa Fluor 647 were used at 1ug/ml concentration in FACS buffer (ThermoFisher Scientific). For western blotting ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... A549 cells (human, lung; CRM-CCL-185, ATCC) were cultivated in DMEM/F-12 medium with Nutrient Mix (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral overexpression of PNUTS was achieved by cloning the full-length human cDNA of PNUTS into pLenti4/v5-DEST (Life technologies). The PP1 non-binding mutant PNUTS (W401A ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 live cells were incubated with the serum from a convalescent donor at the 1:100 dilution in PSB for 30 min followed by the incubation with secondary anti-human IgG antibodies conjugated with PE (1:250, # H10104, ThermoFisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... The human pancreas adenocarcinoma cell line Bx-PC3 obtained from ATCC (ATCC CRL-1687) was grown in RPMI-1640 medium (ThermoFisher Scientific) supplemented with 10% fetal calf serum at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... envelope and one of the transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s lentiviral production protocol ...
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bioRxiv - Cell Biology 2021Quote: ... human female adenocarcinoma of the mammary gland) were maintained in Dulbecco’s Modified Eagle Medium High Glucose (ThermoFisher Scientific, #11-965-118), 10% foetal bovine serum (Bovogen ...
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bioRxiv - Cell Biology 2021Quote: ... Transferrin, Selenite) premix (resulting in 10 µg/mL insulin, 5 µg/mL human transferrin and 30 nM sodium selenite) (Life Technologies). One droplet (10 µL ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Non – metastasis human colon cancer cell line (HT-29) were cultured in Dulbecco’s modified Eagle’s medium (DMEM: Gibco, Thermo Fisher, USA) and metastasis human colon cancer cell line (SW-620 ...
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bioRxiv - Cell Biology 2021Quote: ... HEMs were cultured in Medium 254 supplemented with 1% human melanocyte growth supplement (S-016-5, Cascade Biologics/Thermo Fisher Scientific) and 100 units/mL penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: Genes encoding full-length human TAK1 and Mfn2 were fused with a 5’ hexahistidine-tag by PCR and then cloned into pFastBac1 vector (Life Technologies). Once constructed ...
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bioRxiv - Molecular Biology 2022Quote: ... Whitehead Institute Center for Human Stem Cell Research, Cambridge, MA) (Lengner et al., 2010) were maintained on MEFs in hESC media (DMEM/F12 [Thermo Fisher Scientific] supplemented with 15% fetal bovine serum [FBS] [Hyclone] ...
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bioRxiv - Bioengineering 2022Quote: ... a human keratinocyte cell line featuring constitutive expression of human telomerase reverse transcriptase (hTERT) were cultured in FAD medium (3:1 DMEM : Ham’s F12 Medium (ThermoFisher®, 11320074), supplemented with 1% PS ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Physiology 2022Quote: ... ATCC) and human HEK-293T cells (catalog no. CRL-3216, ATCC) were grown in DMEM (catalog no. 31966-021, ThermoFisher Scientific) supplemented with 10% Fetal Bovine Serum (catalog no ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: ... Primary human CD4+ T cells stimulated with IL-2 alone or with α-CD3/CD28 antibody-coated beads (Gibco/Thermo Fisher) were infected for 5 days ...
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bioRxiv - Microbiology 2022Quote: J774.1 mouse macrophages (ATCC) or primary neonatal normal human dermal fibroblasts (Neo-NHDF) (ATCC) were cultured in 10 mL DMEM (Gibco, ThermoFisher) in T75 flasks (VWR ...
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bioRxiv - Microbiology 2022Quote: ... The human anti-TNFα IgG1 monoclonal antibody was expressed in HEK-293F FreeStyle cell line expression system (Life Technologies, Carlsbad, CA) with co-transfection of vectors encoding p21 ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 20 mins at RT with an anti-human FITC-labelled secondary antibody (dilution 1:100) (Thermo Fisher) for A32 detection ...
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bioRxiv - Microbiology 2022Quote: ... Fibrinogen aggregates were stimulated using 0.2mg/mL of purified fibrinogen from human plasma with an Alexa Fluor 488 conjugate (Invitrogen, Waltham, Massachusetts). Images were collected using an Olympus FluoView FV10i Confocal Laser Scanning Microscope under 60x magnification with an additional 2x zoom ...
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bioRxiv - Immunology 2022Quote: ... and human lung fibroblast (MRC-5 cells, ATCC® CCL-171™) were cultured in Minimum Essential Media (MEM, Life Technologies) supplemented with 4% FBS and 2 mM L-glutamine at 37°C in an atmosphere of 5% CO2.
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1-hTERT (female, human epithelial cells immortalized with hTERT, Cat. No. CRL-4000) from ATCC were grown in DMEM (Life Technologies) or DMEM/F12 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... HRP conjugate (1/4500) (all from Jackson Immunosearch Laboratories, Inc.) or goat anti-human IgG (γ-chain specific) HRP conjugate (1/50 000) (Invitrogen) were added and samples were incubated at 20-24°C for 60 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... MEF dPS-/- (presenilin 1 and 2 knock out) and the human neuroblastoma SH-SY5Y cell lines (ATCC) were grown in DMEM/F12 medium (Thermofisher Scientific) and were supplemented with 10% of FBS ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in a confluent monolayer of human foreskin fibroblasts (HFFs) maintained in Dulbecco’s Modified Eagle medium GlutaMAX™(DMEM+ GlutaMAX™, Gibco) supplemented with 10% foetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... IgG opsonized cells were washed and incubated with 3μg/ml of an APC-conjugated anti-human total IgG secondary Ab (Invitrogen, catalog A21445) at 4°C for 25mins ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell death analysis was performed by staining primary human hepatocytes with Dead Cell Apoptosis Kit with Annexin V FITC and PI (V13242, Thermo Fisher). PI+ve cells were then quantified with the flow cytometer (Fortessa ...