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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were diluted 1/10 from stock 100 μM concentrations and PCR reaction was completed using a 7500 real time PCR system (ThermoFisher, USA) and Sybr Green (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... The quality of the libraries was assessed by quantitative PCR using QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems, USA). The cDNA pools were amplified by a final PCR with the introduction of Illumina I7 indices ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed using SYBR Green (TransStart Tip Green qPCR supermix) with a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Gene expression was normalized using ACT2 as an internal control.
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bioRxiv - Neuroscience 2020Quote: ... PR1/2 and mPRs genes were determined through quantitative PCR (qPCR) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems), and data were analyzed with DataAssist (ABI ...
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bioRxiv - Cell Biology 2021Quote: ... and KDM5D were measured by real-time quantitative PCR using a Power SYBR® Green PCR Master Mix reagent (Applied Biosystems) and normalized to HPRT1 or RPL31 ...
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bioRxiv - Neuroscience 2021Quote: ... MA] and quantitative real-time PCR was performed with ABI Prism using Power SYBR Green PCR Master Mix [ThermoFisher Scientific (Applied Biosystems), Waltham ...
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bioRxiv - Biochemistry 2020Quote: ... MA] and quantitative real-time PCR was performed with ABI Prism using Power SYBR Green PCR Master Mix [ThermoFisher Scientific (Applied Biosystems), Waltham ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR was performed on an ABI Prism® 7900 HT real-time PCR system (Applied Biosystems/Life Technologies, Darmstadt, Germany) in 384-well PCR plates with a total reaction volume of 5μL (2 μL forward and reverse primer mixture ...
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bioRxiv - Genetics 2022Quote: ... qRT-PCR was performed on an ABI Prism® 7900 HT real-time PCR system (Applied Biosystems/Life Technologies, Darmstadt, Germany) in 384-well PCR plates with a total reaction volume of 5μL (2 μL forward and reverse primer mixture ...
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bioRxiv - Neuroscience 2019Quote: ... The PCR amplification was performed using Power SYBR green dye and ViiA 7 Real-Time PCR system (Applied Biosystems, Carlsbad, CA). All primer sets used in the qPCR analysis (Table 3 ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR reaction was run in triplicate for each sample using a Prism VIIA7 real-time PCR system (Thermo Fisher Scientific). Primer sequences are available upon request.
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bioRxiv - Cancer Biology 2019Quote: All Quantitative qPCR was performed with standard PCR conditions for using an Applied Biosystems 7900HT Fast Real Time PCR system (Life Technologies). Real-time PCR reactions were carried out using the SYBR Green PCR master mix (Applied Biosystems part no ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time quantitative PCR reactions were performed using TaqMan Gene Expression Assay Mix and TaqMan Universal PCR Master Mix (ThermoFisher Scientific) with the 7500 Fast Real Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied BiosystemsTM TaqManTM Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed with TaqMan probes in 10 μl of reaction volume using a 7300 Real-time PCR system (Applied Biosystems). The sequences of the probes and primers are listed in Supplemental Table S3.
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed with 2 × RealStar Green Power Mixture (gene star) in a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Three biological replicates with three technical replicates were run for all genes ...
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bioRxiv - Cell Biology 2020Quote: ... Real time qPCR was performed with SYBR® Green PCR mastermix using an ABI PRISM® 7900 PCR machine (Applied Biosystems). Results were normalized to glyceraldehyde-3-phosphate dehydrogenase (Gapdh ...
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bioRxiv - Pathology 2021Quote: The qRT-PCR reaction was performed using the TaqMan Probe-Based Gene Expression Analysis and ABI ViiA7 Real-Time PCR Systems (Life Technologies) as described previously [15] ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was subjected to amplification of selected genes by real-time quantitative PCR using Power SYBR Green PCR Master Mix (Applied Biosystems). Average values from duplicates of each gene were used to calculate the relative abundance of transcripts normalized to HPRT and presented as 2-ΔCT ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA product was diluted 4 times and ~5 ul was used as a template in qRT-PCR using BioRad CFX96 Real-time system and SYBR Green PCR MasterMix (Applied Biosystems). The expression levels of four F.vesca genes FveARF8 ...
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bioRxiv - Immunology 2021Quote: ... combined with cDNA samples in a 96-well PCR plate and run on a 7500 Fast Real–Time PCR system (Applied Biosystems). The gene expression data acquired was analyzed using the comparative 2-ΔΔCT method ...
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bioRxiv - Physiology 2019Quote: ... Gene expression analysis was then performed by quantitative PCR (qPCR) on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems) using the UBR5 mouse primers in Table 1 (also used in the other mouse bioengineered muscle analysis) ...
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bioRxiv - Neuroscience 2019Quote: ... in ultrapure water and subjected to real-time PCR using an Applied Biosystems Model 7900HT with TaqMan Universal PCR Mastermix (Applied Biosystems) with the following probes ...
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bioRxiv - Genomics 2021Quote: ... Quantitative PCR was carried out on a Step One Plus Real-Time PCR System using Fast SYBR Green Master Mix (Applied Biosystems).
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bioRxiv - Genetics 2020Quote: ... Quantitative PCR was done with an Applied Biosystems StepOne Plus Real-Time PCR system with Power SYBR Green PCR Master Mix (Applied Biosystems) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Samples were analyzed by quantitative PCR (qPCR) performed with the 7500 Fast Real Time PCR System with Fast SYBR Green Master Mix (Applied Biosystems). qPCR conditions used ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We ran all qRT-PCR experiments in duplicate on the StepOnePlus™ Real-Time PCR System (Applied Biosystems, Waltham, MA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The amounts of short and long Mad2 mRNAs were quantified by using a real-time PCR system with SYBR green PCR Master Mix (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Standard PCR amplification was performed in triplicate on a Step One Plus Real-Time PCR System (Applied Biosystems; Grand Island, NY) in a final volume of 20 μL containing ∼10 ng of cDNA (1.5 μL of RT product) ...
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bioRxiv - Pathology 2021Quote: ... qPCR analysis of gene expression was performed using QuantStudio 6 Real-Time PCR System and SYBR Green PCR Master Mix (Applied Biosystems). Analysis of relative gene expression was carried out using the comparative CT method (ΔCT ...
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bioRxiv - Developmental Biology 2021Quote: ... and human Oct 4 (For: 5’-CTTGCTGCAGAAGTGGGTGGAGGAA-3’/Rev: 5’-CTGCAGTGTGGGTTTCGGGCA-3’) PCRs were conducted using an ABI 7300 Real Time PCR System (Applied Biosystems). PCR cycling conditions were 95° C for 10 min. ...
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bioRxiv - Cell Biology 2020Quote: ... was used to convert RNA to cDNA and 10 ng used in real-time PCR with Sybr Green PCR Master Mix II (Life Technologies) using primers from human and mouse GAPDH (see above).
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bioRxiv - Cell Biology 2021Quote: ... KapaBiosystems) were used for the qRT-PCR reactions and these were performed with a StepOnePlus Real-Time PCR system (Applied Biosystems). Primers and probes were purchased from Oligomer and Roche Universal Probe Library ...
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bioRxiv - Immunology 2021Quote: ... and PCR was performed on a QuantStudio 3 Real-Time PCR System machine using TaqMan Fast Advanced Master Mix (Applied Biosystems). Data were analyzed using the 2−ΔΔCt method against housekeeping gene Rpl19 and presented as relative expression compared to control ...
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bioRxiv - Immunology 2021Quote: ... The resulting cDNA was used as template for quantitative PCR (qPCR) with the Power SYBR Green reagent on a 7500 Fast Real-Time PCR System (Applied Biosystems). Transcripts were normalized to Rps17 (40S ribosomal protein S17 ...
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bioRxiv - Microbiology 2021Quote: ... TaqMan qPCR was performed in duplicate on the 7500 Real-Time PCR system using 2X PCR Universal Master Mix (Applied Biosystems) and primer/probe pairs specific for HSV-1 US4 ...
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bioRxiv - Cell Biology 2021Quote: ... was performed on the Applied Biosystems QuantStudio 6 Flex Real-Time PCR System using Fast Universal PCR Master Mix and TaqMan Gene Expression assays (Applied Biosystems), listed in Table S1.
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bioRxiv - Genetics 2020Quote: ... The PCR products and their dissociation curves were detected with a 7500 Fast real-time PCR system thermal cycler (Thermo-Fisher Scientific). The template cDNA was omitted in the qRT-PCR negative control ...
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bioRxiv - Genomics 2021Quote: The quantitative PCR (qPCR) assay was performed on a QuantStudio™ 3 Real-Time PCR System (ThermoFisher Scientific Cat. no. A28567) in a 20 μl reaction mixture consisting of Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 ng of digested/undigested DNA was then quantified by real time PCR using a 7500 FAST Applied Biosystems thermocycler with SYBR Green PCR Master Mix (Applied Biosystems) and 100 nM of primer in a 20 μl reaction ...
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bioRxiv - Microbiology 2022Quote: ... 50 ng of cDNA was used to conduct qRT-PCR with SYBR green reaction mix on a Step One Real-Time PCR System (Applied Biosystems). 16S rRNA gene levels were used to normalize expression levels of KKP components in MPAO1 biofilms at different days ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 10 ng cDNA for each sample was used and all qRT-PCR reactions were performed on a ViaA 7 Real-Time PCR System (Life Technologies) using Brilliant II SYBR Green QPCR Master Mix (Agilent) ...
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bioRxiv - Molecular Biology 2022Quote: ... was used for qRT-PCR that was performed with the QuantStudio 3 Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific). The mRNA expression levels were normalized against the respective GAPDH expression in each sample ...
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bioRxiv - Molecular Biology 2022Quote: ... was used for qRT-PCR that was performed with the QuantStudio 3 Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific). The mRNA expression levels were normalized against the respective GAPDH expression in each sample ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) analysis using SYBR green PCR master mix (cat#4309155, Thermofisher Scientific) with sequence specific primers ...
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bioRxiv - Immunology 2022Quote: ... Normalization was performed using nidogen gene and quantitative PCR (qPCR) was performed on a StepOne Plus real-time PCR machine (Applied Biosystems), with the following settings (absolute quantification program) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantitative PCR (qPCR) was performed using a QuantStudio 3 Real-Time PCR System in conjunction with a SybrGreen System (ThermoFisher Scientific) for assessment of the expression of cell cycle- ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR analysis was performed with an Applied Biosystems 7500 Fast Real Time PCR System with TaqMan Universal PCR Master Mix and pre-designed TaqMan gene expression assays (Life Technologies). Relative expression levels were calculated using the comparative cycle threshold method referenced to ACTB.
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bioRxiv - Pathology 2022Quote: ... qRT–PCR was performed using qPCRBIO SyGreen Mix (Protech) and the QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems/Thermo Fisher Scientific Inc. ...
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bioRxiv - Physiology 2022Quote: ... Resulting cDNA was then utilized for qPCR using Taqman Q-PCR probes in QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). Data was analyzed using Microsoft Excel and graphed in GraphPad PRISM v8.