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Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the Superscript III One-Step RT-PCR System (Invitrogen). cDNA was first synthesised with a single primer being added and the second primer added for the PCR step ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR samples were prepared using Taqman 1-Step RT-PCR master mix (Invitrogen) and Taqman gene expression assays (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... to perform RT-PCR using the StepOnePlus RT-PCR system (ABI 7700, Applied Biosystems). We calculated the relative gene expression using the ΔCt method and normalized the data to β-actin (Actb) ...
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bioRxiv - Immunology 2021Quote: ... Beads were re-emulsified in an overlap extension RT-PCR mixture using the SuperScript III RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA) with the incorporation of custom designed TCR cloning primers (Supplementary Table 2)53 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was isolated from wild type N2 animals using trizol and phenol-chloroform extraction followed by RT-PCR using Superscript III One Step RT-PCR system with Platinum Taq DNA polymerase kit (ThermoFisher Scientific cat #: 12574026) to prepare the cDNA ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from N2 animals with Trizol and phenol-chloroform prior to cDNA synthesis using RT-PCR with the Superscript III One Step RT-PCR system with Platinum Taq DNA polymerase kit (ThermoFisher Scientific cat #: 12574026). The resultant PCR product was used as a template for in vitro transcription (IVT ...
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bioRxiv - Microbiology 2023Quote: ... were used as template in a one-step RT-PCR reaction with the SuperScrip IV One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA) and primers that amplify coding sequences for Spike amino acids 614 and 655 (Table 2) ...
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bioRxiv - Molecular Biology 2019Quote: ... 5’-GAGTTGCTGTTAAGTCACAG-3’ in an Eppendorf Mastercycler ep realplex real-time PCR system with DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo Fisher, Waltham, USA). For normalization of Hmgcr expression ...
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bioRxiv - Biochemistry 2020Quote: ... To pool the barcoded samples at equimolar ratio a quantification step by real time PCR using Ion Library TaqMan™ Quantitation Kit (Thermo Fisher Scientific) was realised ...
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bioRxiv - Microbiology 2019Quote: ... RNA levels were determined with Power SYBR® Green RNA-to-CT™ 1-Step Kit using a StepOne Plus Real-Time PCR system (Applied Biosystems). For relative quantification ...
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bioRxiv - Cell Biology 2022Quote: ... 100 ng RNA were used for real-time PCR with the Power SYBR® Green RNA-to-CT™ 1-Step kit (Applied Biosystems) and the CFX96 Touch Real-Time PCR Detection System (Bio-RAD ...
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bioRxiv - Physiology 2023Quote: ... was reverse transcribed with a High Capacity cDNA Reverse Transcription Kit (Applied Biosciences, Carlsbad CA) and amplified by real-time quantitative PCR (qPCR) with SYBR Green (Life Technologies, Carlsbad, CA) (43) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reverse transcription and PCR amplification were performed using TaqMan RNA-to-CT 1-Step Kit and the StepOnePlus™ Real-Time PCR Instrument (Applied Biosystems, Fisher Scientific). All primers used in this study are listed in Table S1.
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bioRxiv - Molecular Biology 2022Quote: ... A serial dilution of input DNA and antibody-bound DNA were prepared from three independent ChIP experiments and analyzed two times using a StepOne Plus real-time PCR system (Thermo Fisher Scientific). PCR cycling conditions are described below ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed with specific primers (Table S1) using the QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was carried out using Fast SYBR Green Master Mix using a QuantStudio 5 real-time PCR sytem (both Applied Biosystems). B2m was used as a housekeeping gene and relative fold changes in Apc and mCherry expression were derived from ∆∆CT ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Lifetech, #43-676-59) on a ViiA 7 Real-Time PCR System (Applied Biosystems). The primers used are listed in Table S2 ...
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bioRxiv - Cell Biology 2020Quote: Quantitative PCR was done on the QuantStudio 6 Flex with the QuantStudio Real-Time PCR software using SYBR green PCR master mix (Applied BioSystems). Primers (see Appendix S2 for primer sequences ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-time q-PCR analyses were carried out using the Powersyber Green PCR Master Mix and QuantStudio 12k flex (Applied Biosystems). Rsp17 and TBP2 served as reference genes using the comparative Ct method ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was diluted 1:5 in water and 2μl was used for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a Quant Studio 5 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... and real-time quantitative PCR was performed using a Viia 7 Real-Time PCR System on 384 custom-made TaqMan microfluidic cards (ThermoFisher, 4342253). CTs were normalised to the average CT values of Gapdh and Hprt ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 ng of digested/undigested DNA was then quantified by real time PCR using a 7500 FAST Applied Biosystems thermocycler with SYBR Green PCR Master Mix (Applied Biosystems) and 100 nM of each primer in a 20 μl reaction ...
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bioRxiv - Genomics 2019Quote: ... The qRT-PCR reactions were performedin 96-well plates using the ABI 7500 fast Real-Time PCR system (Applied Biosystems, USA) with the QuantiFast SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... and MT1H were measured by real-time quantitative PCR using a Power SYBR® Green PCR Master Mix reagent (Applied Biosystems) and normalized to RPL31 ...
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bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
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bioRxiv - Immunology 2021Quote: ... was added to each sample for the PCR reaction and run on the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific). The value of STAT3 Exons 20/21 and Introns 20/21 was calculated as 2ΔCt(GAPDH) ...
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bioRxiv - Developmental Biology 2019Quote: ... qRT-PCR was performed using Thunderbird SYBR qPCR Mix (Toyobo) and QuantStudio™ 12K Flex Real-Time PCR System (Thermo Fisher). The expression values of target genes were normalized by b-actin expression from the same cDNA templates ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR reactions were performed using a Quant Studio 7 Flex Fast Real-Time PCR System (Applied Biosystems by life technologies) and the following Taqman primers (Thermo Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time PCR was performed in a total volume of 20 µL on a Step One Plus real-time PCR system (Applied Biosystems) in 96-well PCR plates (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed using the Power SYBR Green PCR Master Mix or TaqMan Gene Expression Master Mix using a 7500 Real Time PCR machine (Applied Biosystems). All compounds were purchased from Life Technologies (St Aubin ...
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bioRxiv - Microbiology 2019Quote: ... was performed with SYBR Green PCR Master Mix (Applied Biosystem, Foster City, USA) on a 7900 Real Time PCR System (Applied Biosystems). Two biological replicates ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR (qPCR) was performed in duplicate using the FAST SYBR Green dye on the StepOnePlus real-time PCR system (Applied Biosystems). Primer sequences are listed in (Supplementary Table 3).
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bioRxiv - Microbiology 2019Quote: ... according to the Manufacturer’s recommendations and used for qRT-PCR in an Applied Biosystems® 7500 Real-Time PCR Systems (Thermo fisher) as described previously ...
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bioRxiv - Immunology 2019Quote: ... and amplification and detection were performed using the TaqMan Fast Universal PCR Master mix in the AB 7500 FAST Real-Time PCR System (Applied Biosystems). Standard curves for each target gene were generated as previously described (71) ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR assays were performed as TaqMan single-plex assays on the StepOne Plus Real-Time PCR System (Thermo Fisher Scientific), using the manufacturer recommended settings for quantitative and relative expression ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR reactions were performed in triplicate on QuantStudio 6 Flex Real-Time PCR Systems (Cat#4485694, Applied Biosystems, Waltham, USA) using the TaqMan Fast Advanced Master Mix (Cat#4444963 ...
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bioRxiv - Cancer Biology 2019Quote: Real-time PCR reactions were performed in triplicate in 96-well plates in a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). Equal initial amounts of total DNA were amplified in all conditions ...
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bioRxiv - Cell Biology 2021Quote: ... Viral copy number quantification by RT-qPCR was performed using Detection Kit for 2019 Novel Coronavirus (2019-nCoV) RNA (PCR-Fluorescent Probing) (Cat. #DA-930) (China) in a QuantStudio 3 real-time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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Mis6/CENP-I maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosisbioRxiv - Cell Biology 2021Quote: ... The amounts of DNA derived from whole-cell extracts and ChIP samples were assessed via quantitative PCR on the StepOne Real-Time PCR system (Applied Biosystems) using SYBR Green (TOYOBO) ...
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bioRxiv - Pathology 2021Quote: ... qRT-PCR reactions were performed on an ABI Prism 7500 Fast real-time PCR System (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... Quantitative PCR was performed using the Fast SYBR Green Master Mix in a real-time PCR system (Fast 7500; Applied Biosystems). Each data point was repeated three times and normalized for the data for ribosomal protein 49 (RP49) ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was subjected to amplification of selected genes by real-time quantitative PCR using Power SYBR Green PCR Master Mix (Applied Biosystems). Average values from duplicates of each gene were used to calculate the relative abundance of transcripts normalized to HPRT and presented as 2-ΔCT ...
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bioRxiv - Plant Biology 2021Quote: ... Reactions of qRT-PCR were done in a 384-well plate format with 7900HT Fast Real-time PCR System (Applied Biosystems® ...
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bioRxiv - Genomics 2020Quote: Quantitative PCR was done on the QuantStudio6 Flex with the QuantStudio Real-Time PCR software using SYBR green PCR master mix (Applied BioSystems). Primers (see Table E2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR was carried out using Power SYBR Green PCR master mix on a 7900HT Fast Real-Time System (Applied Biosystems) or QuantStudio-6 Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA samples for quantitative Real Time PCR were prepared using a mix of 1X Fast-SYBR Green PCR Master Mix (Applied Biosystems) and the primers listed in Table 4 ...
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bioRxiv - Immunology 2022Quote: ... The presence of genomic SARS-CoV-2 RNA in these samples was evaluated by one-step RT-qPCR in a final volume of 25 μl per reaction in 96-well PCR plates using a thermocycler (7500t Real-time PCR system, Applied Biosystems) as previously described (Melo et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR experiments were performed with SYBR Green Master Mix reagent with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative quantifications were determined with the above primer pairs using delta delta threshold cycle methods with the following primers:
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on the Applied Biosystems ViiA 7 using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The level of ACTIN7 (ACT7 ...
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bioRxiv - Immunology 2022Quote: ... The PCR reaction was prepared according to procedure of PreAmp mix and performed with StepOnePlus Real-Time PCR System (Applied Biosystems).