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Citations for Thermo Fisher :
5101 - 5150 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescence probe amplification was detected by QuantStudioTM 6 Flex Real-Time PCR System (Thermo Fisher Scientific). Data were analyzed by ΔΔCt calculation normalized to mGAPDH ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR was performed using the PowerUp SYBR™ Green Master Mix (ThermoFisher, A25741) and Bio-Rad C1000 Touch Thermal Cycler ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time qPCR was performed using Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific). The primer sequences of BCL6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time qPCR was performed with Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific) and primers of miR-15a ...
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bioRxiv - Microbiology 2024Quote: ... The qPCR analysis was performed using a QuantStudio™ 3 Real-Time PCR System (ThermoFisher Scientific) with the following protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Fluorescence melt curve data was acquired using a QuantStudio 5 Real-Time PCR System (Applied Biosystems) with the following method ...
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bioRxiv - Molecular Biology 2024Quote: ... and ChIP DNA was analyzed using the Quant Studio 3 Real-Time PCR system (Applied Biosystems). The percent input value of the sample paired no antibody mock immunoprecipitation has been subtracted from the percent input obtained from immunoprecipitation with IgG or the indicated specific antibody of the corresponding sample ...
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bioRxiv - Biochemistry 2024Quote: TSA was done in a CFX96 touch-real time PCR using a fluorescent dye from ThermoFisher Scientific (catalogue number 4461146) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Real-Time PCR was performed on a QuantStudio-7 Flex System (Applied Biosystems, Foster City, CA) using Applied Biosystems TaqMan Fast Universal PCR Master Mix and TaqMan probes (specific assays available on request) ...
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bioRxiv - Physiology 2024Quote: ... The qPCR assays were carried out on a QuantStudio 3 Real-time PCR system (Applied Biosystems).
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bioRxiv - Genetics 2024Quote: All qPCR assays were performed using a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2024Quote: ... and 30 s at 72°C) on a 7500 Fast Real-Time PCR System (Applied Biosystems). The target gene expression was normalized to GAPDH ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was carried out using PowerUp SYBR Green Master Mix (Applied Biosystems, 100029284) on a QuantStudio 6 Pro (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were analyzed using a real-time PCR system (Applied Biosciences QuantStudio 3, Thermo Fisher Scientific) using the following cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... The experiment was run on a QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific) and analyzed using the QuantStudio™ Design & Analysis Software v1.5.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... All reactions was performed in triplicates using ViiA 7 Real-Time PCR System (Thermo Fisher, USA). The 2-ΔΔCt method was used to assess relative expression in analyzed tissues using the mean Ct values of selected microRNAs and U6.
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bioRxiv - Cancer Biology 2024Quote: ... mRNA levels were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) using the following PCR primers on a ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). Primer sequences ...
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bioRxiv - Microbiology 2022Quote: All cDNA fragments generated by PCR/RT-PCR were purified using a GeneJET PCR Purification kit (Thermo Scientific) following the manufacturer’s instructions prior to use in TAR ...
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bioRxiv - Immunology 2019Quote: ... Cycling was performed in duplicate using a Step One Plus™ Real Time detection system (Applied Biosystems, Foster City, CA). Gene-specific amplification was normalized to GAPDH as an internal reference gene and gene expression was normalized relative to uninfected MDSC controls using the formula 2-ΔΔCt.
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bioRxiv - Molecular Biology 2021Quote: ... Visual detection was accomplished by imaging the tubes through E-Gel™ Safe Imager™ Real-Time Transilluminator (Thermo Fisher).
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction was performed using the ABI Prism 7000 Sequence Detection System (Life Technologies, Carlsbad, CA). Run method ...
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bioRxiv - Systems Biology 2020Quote: Pooled single cell samples were prepared for multiplex real-time quantitative polymerase chain reaction (RT-qPCR) with the Biomark HD system using VILO III (ThermoFisher) reverse transcription of RNA into cDNA followed by 22 cycles of pre-amplification (Taqman pre-amp master mix ...
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A New Gene Set Identifies Senescent Cells and Predicts Senescence-Associated Pathways Across TissuesbioRxiv - Cell Biology 2021Quote: ... Transcript mRNA levels were determined by RT-qPCR on the ABI Prism 7900HT Real Time System (Applied Biosystems, Carlsbad, CA) using SYBR green (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Pooled single cell samples were prepared for multiplex real-time quantitative polymerase chain reaction (RT-qPCR) with the Biomark HD system using VILO III (ThermoFisher) reverse transcription of RNA into cDNA followed by 22 cycles of pre-amplification (Taqman Pre-amp Master Mix ...
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bioRxiv - Physiology 2023Quote: ... Transcript mRNA levels were determined by rt-qPCR on the ABI Prism 7900HT Real Time System (Applied Biosystems, Carlsbad, CA) using murine TaqMan assays (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... One µg of total RNA was retrotranscribed into complementary cDNA using an RT-PCR kit (High capacity cDNA rt Kit, Applied Biosystems). Quantitative PCRs were carried out in triplicate of each sample using 40 ng of cDNA per well ...
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bioRxiv - Microbiology 2022Quote: ... and gene-specific primers were used to perform quantitative reverse transcription-PCR (qRT-PCR) on 5 ng/μL cDNA in a Real-Time PCR System (Applied Biosystems ViiA 7, USA). Table S2 lists the primers used in qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using the Applied Biosystems StepOnePlus Real-Time PCR System with SYBR Green PCR master mix (Life Technologies, cat. no. 4364344) Primer sets used included ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the Superscript III One-Step RT-PCR System (Invitrogen). cDNA was first synthesised with a single primer being added and the second primer added for the PCR step ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR samples were prepared using Taqman 1-Step RT-PCR master mix (Invitrogen) and Taqman gene expression assays (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... to perform RT-PCR using the StepOnePlus RT-PCR system (ABI 7700, Applied Biosystems). We calculated the relative gene expression using the ΔCt method and normalized the data to β-actin (Actb) ...
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bioRxiv - Immunology 2021Quote: ... Beads were re-emulsified in an overlap extension RT-PCR mixture using the SuperScript III RT-PCR Kit (Thermo Fisher Scientific, Waltham, MA) with the incorporation of custom designed TCR cloning primers (Supplementary Table 2)53 ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was isolated from wild type N2 animals using trizol and phenol-chloroform extraction followed by RT-PCR using Superscript III One Step RT-PCR system with Platinum Taq DNA polymerase kit (ThermoFisher Scientific cat #: 12574026) to prepare the cDNA ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from N2 animals with Trizol and phenol-chloroform prior to cDNA synthesis using RT-PCR with the Superscript III One Step RT-PCR system with Platinum Taq DNA polymerase kit (ThermoFisher Scientific cat #: 12574026). The resultant PCR product was used as a template for in vitro transcription (IVT ...
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bioRxiv - Microbiology 2023Quote: ... were used as template in a one-step RT-PCR reaction with the SuperScrip IV One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA) and primers that amplify coding sequences for Spike amino acids 614 and 655 (Table 2) ...
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bioRxiv - Molecular Biology 2019Quote: ... 5’-GAGTTGCTGTTAAGTCACAG-3’ in an Eppendorf Mastercycler ep realplex real-time PCR system with DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo Fisher, Waltham, USA). For normalization of Hmgcr expression ...
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bioRxiv - Biochemistry 2020Quote: ... To pool the barcoded samples at equimolar ratio a quantification step by real time PCR using Ion Library TaqMan™ Quantitation Kit (Thermo Fisher Scientific) was realised ...
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bioRxiv - Microbiology 2019Quote: ... RNA levels were determined with Power SYBR® Green RNA-to-CT™ 1-Step Kit using a StepOne Plus Real-Time PCR system (Applied Biosystems). For relative quantification ...
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bioRxiv - Cell Biology 2022Quote: ... 100 ng RNA were used for real-time PCR with the Power SYBR® Green RNA-to-CT™ 1-Step kit (Applied Biosystems) and the CFX96 Touch Real-Time PCR Detection System (Bio-RAD ...
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bioRxiv - Physiology 2023Quote: ... was reverse transcribed with a High Capacity cDNA Reverse Transcription Kit (Applied Biosciences, Carlsbad CA) and amplified by real-time quantitative PCR (qPCR) with SYBR Green (Life Technologies, Carlsbad, CA) (43) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reverse transcription and PCR amplification were performed using TaqMan RNA-to-CT 1-Step Kit and the StepOnePlus™ Real-Time PCR Instrument (Applied Biosystems, Fisher Scientific). All primers used in this study are listed in Table S1.
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bioRxiv - Molecular Biology 2022Quote: ... A serial dilution of input DNA and antibody-bound DNA were prepared from three independent ChIP experiments and analyzed two times using a StepOne Plus real-time PCR system (Thermo Fisher Scientific). PCR cycling conditions are described below ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed with specific primers (Table S1) using the QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was carried out using Fast SYBR Green Master Mix using a QuantStudio 5 real-time PCR sytem (both Applied Biosystems). B2m was used as a housekeeping gene and relative fold changes in Apc and mCherry expression were derived from ∆∆CT ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Lifetech, #43-676-59) on a ViiA 7 Real-Time PCR System (Applied Biosystems). The primers used are listed in Table S2 ...
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bioRxiv - Cell Biology 2020Quote: Quantitative PCR was done on the QuantStudio 6 Flex with the QuantStudio Real-Time PCR software using SYBR green PCR master mix (Applied BioSystems). Primers (see Appendix S2 for primer sequences ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-time q-PCR analyses were carried out using the Powersyber Green PCR Master Mix and QuantStudio 12k flex (Applied Biosystems). Rsp17 and TBP2 served as reference genes using the comparative Ct method ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was diluted 1:5 in water and 2μl was used for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a Quant Studio 5 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... and real-time quantitative PCR was performed using a Viia 7 Real-Time PCR System on 384 custom-made TaqMan microfluidic cards (ThermoFisher, 4342253). CTs were normalised to the average CT values of Gapdh and Hprt ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 ng of digested/undigested DNA was then quantified by real time PCR using a 7500 FAST Applied Biosystems thermocycler with SYBR Green PCR Master Mix (Applied Biosystems) and 100 nM of each primer in a 20 μl reaction ...