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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... incubated for 5 min at 37 °C with pre-warmed TrypLE 1X Express (Gibco), fixed for 10 min at room temperature with 1.6% paraformaldehyde (PFA ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were stained with 5 μg/ml of Hoechst 33342 (nuclear dye) (Life Technologies) and 5 μg/ml of propidium iodine (vital dye ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by claudin-5 (Invitrogen, Grand Rapids, NY, cat # 35-2500) IHC at a 1:50 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine RED Kit (Leica ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with fetal bovine serum (FBS) 5-10% and appropriate antibiotics (all Thermo Fisher). HeLa-CD4-shRNA-REAF were selected for resistance to puromycin in media supplemented with 10μg/ml puromycin (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... The samples were then stained for 5 min with the SYTO 9 (ThermoFisher Scientific) at final concentration of 0.02 μg/ml (green fluorescence ...
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bioRxiv - Microbiology 2020Quote: ... Acceptor cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate) (Thermo Scientific) for 30 minutes according to the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3.3 nM of 5-chloromethylfluoresecein di-beta-D-galactopyranoside (CMFDG) (Invitrogen, Waltham, MA, USA) was added to the reaction solution ...
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bioRxiv - Systems Biology 2020Quote: ... Quantitative 5′end phosphorylation of RNA was accomplished by adding ATP (Thermo Fisher Scientific) to a concentration of 100 μM for 5 min.
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Tau proteins were biotinylated with 5 molar excess of NHS-biotin conjugates (Thermofisher) during 4 hours at 4 °C ...
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bioRxiv - Systems Biology 2019Quote: ... FACS purified DN1 cells were stained in 5 μM Cell Trace Violet (Molecular Probes) for 7 minutes at 37°C in HBSS and washed 2X with whole medium before being plated in 96-well OP9-DL1 co-cultures ...
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bioRxiv - Synthetic Biology 2020Quote: ... Labelled cells were added to streptavidin beads (5 µl, Dynabeads, MyOne C1, Thermo Fisher) in binding buffer (TN-DBT ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 5% of Alexa Fluor (488nm or 568nm) conjugated Annexin V (Life Technologies LTD) for 10min ...
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bioRxiv - Immunology 2020Quote: ... and 5 mg/mL Sulfo-NHS (N-hydroxysulfosuccinimide; Thermo Fisher Scientific; Waltham, MA, USA). Activated microspheres were washed twice in 0.05 M MES (2-(N-morpholino ...
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bioRxiv - Cancer Biology 2019Quote: ... The analytical column was protected by a 5 mm guard column (Thermo Fisher Scientific). Chromatographic separation was performed at 250 nL/min during a linear gradient beginning from 95% A/5% B to 68% A/32% B in 90 min ...
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bioRxiv - Biochemistry 2019Quote: Gene 5 was cloned between the BamHI and HindIII sites of plasmid pSE380 (Invitrogen) to form recombinant plasmid pMS11 (26) ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... 5% CO2 and 90% humidity in Dublecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 9% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... we used 5 μg plasmid DNA and Lipofectamine 3000 (Life Technologies, Grand Island, NY) to transfect 5×106 cells in 10 mL DMEM supplemented with 10% FBS ...
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bioRxiv - Evolutionary Biology 2019Quote: Modified 5’ RACE was carried out using the GeneRacer Kit (Invitrogen by life technologies) according to the manufacturer’s instructions without the enzymatic pretreatment of the RNA ...
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bioRxiv - Immunology 2019Quote: ... coli (5×108 CFU/mL) (7 μg/mL, BacLight, Molecular Probes, Eugene, OR, USA). Fluorescence was assessed after 60 min (37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The wild-type or mutant 5-HT2C was cloned into the pcDNA3.1 vector (Invitrogen) for transfection ...
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bioRxiv - Biophysics 2020Quote: ... Both cell lines were maintained at 37°C and 5% CO2 in DMEM (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... CD4 (RM4-5) purchased from eBioscience and Granzyme B (GB12) purchased from Life Technologies. Staining of cytoplasmic and nuclear antigens was performed using the Fixation/Permeabilization kit (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... 5 µl of 3µM siRNA was mixed with 1.75 µl of Lipofectamine 2000 (ThermoFisher), 1.75 µl H2O and 3 µl of Opti-MEM (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Triethylamine and 5-hehyn-1-ol 97% were purchased from Acros Organics (Geel, Belgium). Deuterium oxide (D2O ...
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bioRxiv - Genomics 2021Quote: ... 5% CO# in MEF medium (high glucose DMEM with HEPES (Thermo Fisher Scientific, #12430054), 10% FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: ... Then it was incubated in 5 mg/ml Dispase II (Thermo Fisher Scientific, 17105041) in HBSS (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated with 5-ehtynyl-2’-deox-yuridine (EdU: Invitrogen, Thermo Fisher Scientific) at 10 µM ...
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bioRxiv - Cell Biology 2020Quote: ... and cultured to form hEBs for 5 days in KO-DMEM (Gibco, A12861-01) supplemented with 20% KOSR (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μM DAF-FM diacetate and 1 μM LysoTracker Red DND-99 (Life Technologies), respectively ...
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bioRxiv - Physiology 2021Quote: ... Each reaction consisted of 5 μL SYBRR green dye (Thermo Fisher Scientific, Vilnius, Lithuania), 0.5 μL of 2 μM forward and reverse primers ...
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bioRxiv - Immunology 2020Quote: ... Cell division was assessed by staining cells with 5 μM CellTrace Violet reagent (Thermofisher) and flow cytometric analysis (104 single cell events ...
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bioRxiv - Biochemistry 2021Quote: ... 10 % FBS and 5 µM retinal in a CO2-independent medium (Thermo Fisher Scientific). Following 2 hours equilibration at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... and ligands were diluted to appropriate concentrations (5 x final concentration) in HBSS (Thermofisher) supplemented with 20 mM HEPES (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: Cells on glass coverslips were loaded with Fura2-AM (5 μM, Thermo Fisher Scientific) for 30min ...
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bioRxiv - Immunology 2021Quote: ... KPC cells (34) were cultured in DMEM supplemented with 5% FBS (Gibco 26140-079), 2 mM Glutamine (Gibco 25030-081 ...
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bioRxiv - Molecular Biology 2021Quote: The membrane was incubated in 5 mL SEA BLOCK Blocking Buffer (Thermo Scientific, #37527) for 30 min at RT in a dark container on a rocking mixer ...
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bioRxiv - Synthetic Biology 2021Quote: ... We used 5 μL of pre-stained protein ladder (26619, Thermo Scientific, MA, USA). Protein transfer to the PVDF membrane was performed following manufacturer’s instructions for the membrane kit (Trans-Blot Turbo Midi 0.2 μm PVDF ...
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bioRxiv - Neuroscience 2021Quote: ... at 37°C/5%CO2 and resuspended in E8 basal medium (ThermoFisher #A15170-01), supplemented with primocin (0.1 μg/ml) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2×10^5 individualized hESCs were resuspended in 10 μL Resuspension Buffer R (Invitrogen) containing CRISPR ribonucleoproteins (Cas9 protein+sgRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Explant were cultured at 37°C in 5% CO2 atmosphere (Thermo Scientific Midi 40), either during 24h for the Japanese quail (i.e. ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM DTT and 5 U/mL SUPERase.In RNase Inhibitor(Thermo Fisher Scientific #AM2696)) on ice and post-nuclear extracts placed on top of 10-50% weight/volume sucrose gradients containing the same buffer (apart from no Triton X-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... complete EDTA-free protease inhibitor mixture (0.5 tablet for 5 mL, Thermo Fisher Scientific), phosSTOP phosphatase inhibitor mixture (0.5 tablet for 5 mL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with each plasmid for 5 h using Lipofectamine 2000 (Life Technologies) and further incubated for 24-48 h ...
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bioRxiv - Genetics 2021Quote: ... Samples amplified for 5 cycles were then subjected to qPCR (SYBR Green, Thermo Fisher). The additional number of cycles required was the number of cycles required to reach one quarter of the maximal fluorescence intensity in the first 5 cycles ...
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bioRxiv - Biochemistry 2020Quote: ... 5 fraction collection passes were performed on Dionex Ultimate 3000 HPLC (Thermo Scientific, USA) using Nucleodur HTec C18 VP 125/10 column (Macherey-Nagel ...
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bioRxiv - Microbiology 2020Quote: ... Sertoli cells were maintained at 32°C with 5% CO2 in DMEM/F12 (Gibco) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were stained for 5 min with Coomassie SimplyBlue SafeStain (Thermo Fisher Scientific) prior in-gel trypsin proteolysis performed as described in Hartmann et al ...
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bioRxiv - Immunology 2021Quote: ... 3.8 x 106 293T cells were plated in 10 ml DMEM + 5% Glutamax (Thermofisher) on a 10 cm plate ...
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bioRxiv - Immunology 2021Quote: ... naive CD4+ T cells were labelled with 5 μM CFSE (Molecular Probes, Eugene, OR) in PBS containing 5% FBS for 5 min at RT and washed 3 times.