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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... TaqMan assays were run on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) using the comparative Ct method.
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bioRxiv - Microbiology 2020Quote: ... Confluent monolayers of cells were dissociated with 5 ml of TrypLE (Thermo Fisher Scientific) and pelleted ...
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bioRxiv - Cell Biology 2021Quote: ... France) and cultured at 37 °C in 5% CO2 in Gibco Opti-MEM (Invitrogen), plus 10% fetal bovine serum and 1% penicillin/streptomycin.
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bioRxiv - Physiology 2021Quote: ... Sample plates were run using a QuantStudio 5 Real-Time PCR System (Applied Biosystems) using a 40-cycle amplification protocol.
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bioRxiv - Microbiology 2020Quote: ... HIOs were cultured in group of 5 per well using 4-well plates (ThermoFisher). Lumens of individual HIOs were microinjected with glass caliber needles with 1 μl of PBS or different strains of Salmonella (105 CFU/HIO or 103 CFU/HIO for 24h time point experiments) ...
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bioRxiv - Bioengineering 2021Quote: ... and 5 µL of Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific K0221). Samples were prepared on ice and arranged in a MicroAmp 48-well reaction place (Applied Biosystems 4375816) ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate) (Thermo Scientific) for 20 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lines were maintained at 37°C/ 5% CO2 in RPMI 1640 Medium (Gibco) supplemented with 10% FBS and 2% Penicillin/Streptomycin ...
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bioRxiv - Immunology 2021Quote: ... 94°C 5 min) with 4 μl of RNA and random hexamers (Thermofisher scientific). A PCR was further performed based on the protocol established by Tiller et al (Tiller et al. ...
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bioRxiv - Immunology 2020Quote: ... CD8+ cells were then stimulated with anti-CD3/CD28 Dynabeads 5 μL/mL (Invitrogen) and cultured for 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... n = 5 male HR mice was homogenized in TRIzol Reagent (#15596018, Thermo Fisher Scientific) and Zirconium beads (#Zr0B05-RNA ...
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bioRxiv - Cancer Biology 2021Quote: MEFs were incubated with 5 μg/mL DQ Red BSA (Thermo Fisher Scientific, D12051) for 1 h and washed twice with 37°C PBS ...
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bioRxiv - Biochemistry 2020Quote: ... 110 rpm and 5% CO2 in Gibco FreeStyle F17 expression media (Thermo Fisher Scientific) supplemented with 8 mM Glutamine and 0.1% Pluronic F68 (PAN Biotech) ...
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bioRxiv - Neuroscience 2020Quote: ... Amplification of 5’ cDNA ends was performed using the GeneRacer™ Kit (Life Technologies) in accordance with manufacturer instructions ...
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bioRxiv - Bioengineering 2021Quote: ... 6-well plates were first coated with vitronectin (5 μg/mL, Thermo Fisher, USA) followed by a coating with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Bioengineering 2020Quote: ... CD8+ T cells were cultured in a 1:1 mix of Aim 5 (Gibco) and RPMI ...
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bioRxiv - Molecular Biology 2022Quote: ... coli isolates were taken and cultured in 5 mL LB media (Thermofisher Scientific, USA) and grown overnight at 37 ºC at 200 rpm ...
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bioRxiv - Neuroscience 2022Quote: ... 110 rpm and 5% CO2 in Gibco FreeStyle F17 expression media (Thermo Fisher Scientific) supplemented with 8 mM Glutamine and 0.1% Pluronic F68 (PAN Biotech) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were grown at 37°C and 5% COand passaged in DMEM (ThermoFisher #10566016) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using the QuantStudio 5 Real-Time PCR Systems (Applied Biosystems). An initial enzyme activation step of 2 min at 50°C and denaturation step of 5 min at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... were grown at 37°C with 5% CO2 in DMEM (Fisher Scientific, Waltham, USA), supplemented with 10% Fetal Bovine Serum (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and Renilla luciferase at a 1:30:1:5 ratio using Lipofectin (Thermo Fisher) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Cells were spun down at 300g for 5 minutes and washed in DPBS (Gibco). K562 cells were resuspended in 100 uL of DPBS containing Zombie aqua fixable viability dye (Biolegend ...
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bioRxiv - Bioengineering 2019Quote: ... cells were passaged 1:5 to uncoated Petri dishes by adding trypLE (Life Technologies) for 5 minutes to dissociate the cells before being resuspended in growth medium and plated.
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bioRxiv - Immunology 2019Quote: Naïve T cells were labeled with 5 μM of carboxyfluorescein succinimidyl ester (CFSE, ThermoFisher). Labeled cells were cultured in RPMI 1640 media and supplemented with 10% FBS as well as αCD3/CD28 coated Dynabeads™ (Thermo ...
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bioRxiv - Biochemistry 2019Quote: ... Integrants were selected based on resistance to 1.5mg/ml 5-fluoroorotic acid (Fisher Scientific) and validated by whole-cell PCR using primers homologous to endogenous flanking sequences in combination with those within the ORF ...
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bioRxiv - Microbiology 2019Quote: ... for 5 minutes followed by mounting in ProLong Glass Antifade Mountant (Thermo Fisher Scientific) or with DAPI-containing VECTASHIELD mounting medium (Vector Labs ...
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bioRxiv - Microbiology 2019Quote: ... Blocks were sectioned to 5 um thickness and applied to Ultrastick slides (Thermo Scientific). Sections were stored at room temperature until FISH was performed.
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bioRxiv - Cancer Biology 2020Quote: ... 5 µL was injected in a nanoRSLC-Q Exactive PLUS (RSLC Ultimate 3000) (ThermoFisher). Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18 ...
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bioRxiv - Cancer Biology 2020Quote: ... counterstained for 5 mins with DAPI and mounted in ProLong Gold (Life Technologies, #P36930). Secondary antibodies used were ...
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bioRxiv - Immunology 2019Quote: ... MRC5t cells were supplemented with 5 μg/mL hygromycin B (Thermo Fisher Scientific; 10687010) to maintain hTERT expression ...
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bioRxiv - Neuroscience 2019Quote: ... non-specific binding sites were blocked with PBS containing 5% BSA (Fisher Scientific, AAJ1085718) and 0.3 M glycine ...
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bioRxiv - Cell Biology 2019Quote: ... counterstained with DAPI (1:2000) for 5 minutes and mounted in ProLong Gold (Invitrogen). Actin was visualized by phalloidin-AF647 staining (Life Technologies ...
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bioRxiv - Physiology 2019Quote: ... Cells were maintained under standard conditions (37°C, 5% CO2) in M199 medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Zoology 2020Quote: ... 30 μL of DNase I solution containing 5 U of DNase I (Thermo Scientific) was added to the spin column for 20 min at room temperature ...
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bioRxiv - Evolutionary Biology 2019Quote: ... washed in MOPST for 5 minutes and mounted using ProlongGold antifade reagent (Molecular Probes). All samples were observed using the ZEISS LSM 710 META confocal microscope ...
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bioRxiv - Molecular Biology 2020Quote: ... transfected cells were incubated with 5 μM CellROX Oxidative Stress Reagent (Thermo Fisher Scientific) for 30 min at 37°C prior to fixation with paraformaldehyde ...
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bioRxiv - Cancer Biology 2019Quote: ... counterstained for 5 min with DAPI and mounted in ProLong Gold (Life Technologies, #P36930). Secondary antibodies used were ...
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bioRxiv - Physiology 2020Quote: ... MitoTracker Red CMXRos (5 μM, 30 minutes, 37°C) (Molecular Probes, Invitrogen, Paisley, UK), or TMRM (Tetramethylrhodamine ...
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bioRxiv - Physiology 2020Quote: ... MitoTracker Red CMXRos (5 μM, 30 minutes, 37°C) (Molecular Probes, Invitrogen, Paisley, UK), or TMRM (Tetramethylrhodamine ...
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bioRxiv - Cell Biology 2020Quote: ... silencer siRNAs (5 nM) for indicated genes or Negative Control siRNA #1 (Ambion, AM4611) were transfected with Lipofectamin RNAi MAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... the growth media was replaced with FluoroBrite DMEM medium supplemented with 5% FBS (ThermoFisher). Image acquisition was performed in a full environmental enclosure (37°C ...
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bioRxiv - Molecular Biology 2020Quote: Larvae were immersed in egg water containing 5 μM LysoTracker Deep Red (L12492, ThermoFisher) for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... the L-AN-5 cells were stimulated with NGF (200 ng/ml) (Thermo Scientific) for 30 min and subsequently harvested for RNA and protein purification.
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bioRxiv - Physiology 2020Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 15% FBS (Atlas Biologicals) ...
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bioRxiv - Physiology 2020Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 15% FBS (Atlas Biologicals) ...
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bioRxiv - Cell Biology 2019Quote: ... and blocked for at least 20 min with 5% normal horse serum (NHS; ThermoFisher) diluted in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.4 μl UTP-X-Cy3 (5 mM) and 0.4 μl RiboLock RNase Inhibitor (ThermoFisher) and was incubated for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was recovered by using 5 volumes of TRIzol™ LS Reagent (Thermo Fisher). Equal volume of RNA from each sample was used for the reverse transcription ...
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bioRxiv - Pathology 2020Quote: Frozen mouse tissues were sectioned at 5 µm (CryoStar NX50 OP, Thermo Scientific, Germany). After blocking and permeabilization with 10% normal goat serum and 0.2% Triton X-100 in PBS at room temperature for 1 h ...