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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... was used to assess the number of cycles necessary to amplify libraries to the concentration needed for sequencing by amplifying 1 uL of library with LabTAQ Green Hi Rox master mix (Labtech) and adapter-targeted primers on a StepOnePlus Real-Time PCR system (Thermofisher Applied Biosystems). Indexing PCR involved double indexing (Kircher et al ...
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bioRxiv - Plant Biology 2024Quote: ... Constructs were cloned into a Gateway pDest-CTD-His vector via a pDONR intermediate and BP /LR clonase (all reagents Thermo Fisher) following the manufacturer’s instructions (Hartley ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of His-GLPR at 25 μM was incubated with 20x molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Scientific) following supplier instructions ...
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bioRxiv - Genetics 2023Quote: ... The blots containing yeast Nap1 were treated similarly but incubated with mouse anti-HIS primary antibody (Thermo Fisher Scientific, Waltham, MA) and HRP-conjugated sheep anti-mouse secondary antibody (Kindle biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... The 6X His tagged extracellular domain of hPD-L1 WT or 2HA proteins were expressed in the ExpiCHO cell system (ThermoFisher Scientific) and purified by the Ni-NTA agarose (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA fragment encoding the full-length Cckbr1 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The primers used here are listed in Extended Data Table 3 ...
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Microbiology 2023Quote: ... Extended products were purified by alcohol precipitation followed by dissolving in Hi-Di formamide and analysing in an ABI3700 automated DNA Analyzer (Applied Biosystems). Raw sequences are assumable and aligned using NCBI blast and submitted to NCBI databank for accession numbers ...
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bioRxiv - Microbiology 2023Quote: ... or pCDNA SARS-CoV-2 ORF7a-V5/His (WT/H47Y; 0.75 μg) or empty vector using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... monocytes/macrophages (CD3-CD19-NKp46-CD11b+Gr1loLyC6int/hi) and neutrophils (CD3-CD19-NKp46- CD11b+Gr1+LyC6int) were counted using a Countess II Automated Cell Counter (Thermo Fisher) and then combined and processed as one sample per mouse ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were digested with EcoRI and XbaI and inserted into the mammalian expression vector pcDNA-V5-HIS-B (Invitrogen Corp.) that had been cut with the same enzymes ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μl of each diluted and multiplexed PCR product was then added to 10 μl of Hi-Di Formamide and 0.1 μl of GeneScan 500 LIZ Size Standard (Applied Biosystems Inc.) to prepare for genotyping ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Microbiology 2020Quote: 3 ⨯106 cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
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bioRxiv - Immunology 2019Quote: ... Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185) were grown in 10% FCS with 1% L-glutamine in Hams/F-12 media (Gibco).
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bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
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bioRxiv - Biochemistry 2020Quote: ... The absorbance of each well at 450 nm was measured using a Multiskan FC microplate photometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... The culture medium was removed and replaced with RPMI 1640 medium with 10% CS-FCS (Gibco® Life Technologies) and cells were incubated with or without 50 nM T3 for another 3 days ...
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bioRxiv - Cancer Biology 2019Quote: ... The culture medium was removed and replaced with RPMI 1640 medium with 10% CS-FCS (Gibco® Life Technologies) and cells were incubated with or without 50 nM T3 for another 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... The amount of ingested blue dye in the supernatant was measured spectrophotometrically at 620 nm (Fisher Scientific Multiskan FC). Sample readings were normalized to the number of flies.
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bioRxiv - Microbiology 2020Quote: rS1-Fc was expressed in Chinese hamster ovary cells (Free Style CHO-S Cells by Thermo Fisher Scientific, # R80007) in CHO-S-SFM media with hypoxanthine and thymidine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... EVs were resuspended in D-MEM (0 % FCS) + fluorescent Bodipy Phosphocholine analogue at 2 μg/mL (D3793, Thermofisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: Cryosections were permeabilized and blocked for 1 h at RT in 0.1% PBTx and 10% fetal calf serum (FCS; Invitrogen), and incubated O/N at 4 °C with the primary antibodies in 0.1% PBTx plus 1% FCS ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (P/S, 10.000 U/ml, Gibco). Jurkat ...
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bioRxiv - Microbiology 2021Quote: ... or 20% (THP-1) (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (10.000 U/ml, Gibco). THP-1 monocytes were treated with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA ...
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bioRxiv - Cell Biology 2021Quote: Dulbecco’s modified Eagle’s medium (DMEM) and DMEM-F12 containing 10% heat-inactivated fetal calf serum (FCS) (Thermo Fisher Scientific) were used to culture human HEK293T ...
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bioRxiv - Immunology 2022Quote: ... Extracellular antibodies were diluted in FACS buffer (2% FBS and 5mM EDTA in PBS) with Fc Block (Life Technologies) and incubated for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... cell pellet was re-suspended in ice-cold proliferation growth medium (PGM) containing DMEM supplemented with 10% FCS (Invitrogen), 10% horse serum (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... and grown for 4 days on untreated tissue culture plates in a humidified incubator with 5% CO2 at 37°C with culture medium (RPMI, 10% heat-inactivated FCS, 50 units/mL penicillin; 50μg/ml streptomycin -GIBCO) supplemented with 50 ng/ml rhM-CSF (Sino Biologicals) ...
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bioRxiv - Microbiology 2022Quote: ... with 7⍰.5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000μg.mL−1 respectively; Life Technologies) and supplemented with 1⍰% non-essential amino acids (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (Dulbecco’s phosphate-buffered saline [Gibco] supplemented with 2% fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse embryonic fibroblasts (MEF) were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS, GIBCO), 10 U/ml penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... Germany) were cultivated in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...
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bioRxiv - Microbiology 2021Quote: ... J/4320/15) and the absorbance at 450 nm was read on a Multiskan FC Microplate Photometer (Thermo Scientific). Titres were determined by curve fitting data to a 4PL curve in GraphPad Prism 9 and accepting only data which produced an R2 value of >0.95 ...
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bioRxiv - Microbiology 2021Quote: ... 30-2003, ATCC) supplemented with 10% heat-inactivated fetal calf serum (FCS, Hyclone, USA) and 1% pen/strep (Invitrogen) at 37°C in a humidified 5% CO2 incubator (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... These cells were washed with PBS and then resuspended in PBS supplemented with 2% FCS or FBS and 5µM CM-H2DCFDA probe (Invitrogen) and incubated in the dark for 20min at 37°C under 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... with 7 .5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... They were cultured in RPMI 1640 medium supplemented with l-glutamine and 10% FCS (v/v) (Gibco, Massachusetts, USA). Vδ2+ γδ T cell expansion was stimulated using 5 μM zoledronic acid (Actavis ...
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bioRxiv - Cell Biology 2022Quote: ... The absorbance of each well was measured with a microplate reader (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA) set to 450 nm ...
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bioRxiv - Immunology 2020Quote: ... pH 7.2) resuspended and incubated for 30 min in FACS buffer supplemented with Fc gamma receptor CD16/CD32 antibodies (ThermoFisher). Cells were then incubated with Alexa fluor 488-conjugated antibodies against F4/80 or rat IgG2a kappa isotype control ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were immersed in 2ml dissociation solution (2% FCS-PBS solution with approximate 145U/ml type 4 Gibco collagenase) and incubated at 37°C for 30 minutes ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
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bioRxiv - Immunology 2021Quote: ... in R10 media (RPMI 1640 with 10% FCS, 1% penicillin/ streptomycin, and 2 mM L-glutamine, Thermo Fisher Scientific) at 37°C in 5% CO2 to differentiate quiescent MBCs into mASCs ...
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bioRxiv - Immunology 2021Quote: ... The hACE-2-Fc content in the supernatant was quantified by ELISA using 96-Well MaxiSorp Immuno plates (ThermoFisher) coated overnight with 200 ng/well of RBD219-WT protein ...