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Citations for Peprotech :
1601 - 1650 of 1701 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
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bioRxiv - Immunology 2021Quote: ... Th17 cell cultures received 5 ng/mL TGF-β and 50 ng/mL IL-6 recombinant and 20 µg/mL anti-IL-4 and 100 µg anti-IFN-γ (PeproTech). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... qNSCs were treated with aNSC media with the addition of 0.5 μg/mL of the BMP-4 antagonist noggin (PeproTech 120-10C) to more reproducibly induce quiescence exit.
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cancer Biology 2023Quote: ... with 100 μl of processed CM in the presence or absence of anti-LIF antibody (4 μg/ml) or recombinant mouse LIF (0.1 μg/ml; Peprotech, 250-02). Spheroids were quantified after 8 d culture.
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Cell Biology 2024Quote: ... centrifuged and resuspended in satellite cell growth medium (SCGM: F-10 Ham’s medium supplemented with 20% FBS, 4 ng/ ml FGF2 (PeproTech; 100-18B), 1% penicillin–streptomycin and 10mM HEPEs) ...
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bioRxiv - Neuroscience 2022Quote: ... coated 6 well plates at 200,000 cells/well in 2 ml/well StemFlex supplemented with ROCK inhibitor Y-27632 (5 mM, PeproTech) to inhibit apoptosis ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were cultivated on 0.2 % gelatine-coated plates and were maintained in proliferation medium supplemented with 5 ng/ml bFGF (Peprotech) every second day (Rahman et al. ...
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bioRxiv - Immunology 2021Quote: ... (2) treated with recombinant mouse IL-12 (5 ng/ml) and IL-18 (25 ng/ml, both from Peprotech), (3 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Molecular Biology 2022Quote: ... with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, 20 ng/ml TPO, PeproTech Cat AF-300-18) and 1% HEPES (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Cell Biology 2020Quote: ... and resuspended in macrophage complete medium (DMEM/F12, 10% FBS, 1% penicillin/streptomycin, 100 U/ml recombinant murine M-CSF; Peprotech, Rocky Kill, NJ; Cat# 315-02). 5 ml of macrophage complete medium was added on day 3 ...
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bioRxiv - Bioengineering 2020Quote: ... and resuspended in macrophage complete medium (DMEM/F12, 10% FBS, 1% pen/strep, 100 U/ml recombinant murine M-CSF; Peprotech, Rocky Kill, NJ; Cat# 315-02). 5 ml of macrophage complete medium was added on day 3 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Immunology 2020Quote: ... and Th17 (anti-IFN-γ-10μg/ml, anti-IL-4–10μg/ml, IL-6-Peprotech,20ng/ml, TGF-β-Peprotech,2ng/ml) polarizing conditions for 4 days ...
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bioRxiv - Immunology 2020Quote: ... and Th17 (anti-IFN-γ-10μg/ml, anti-IL-4–10μg/ml, IL-6-Peprotech,20ng/ml, TGF-β-Peprotech,2ng/ml) polarizing conditions for 4 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in the absence of EGF and FGF and with the addition of either 10 ng/mL BMP-4 (Peprotech; #315-27) or 1% or 2% foetal bovine serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Systems Biology 2024Quote: ... Osteogenic differentiation was initiated on Day 0 by culturing in osteogenic differentiation medium consisting of growth medium and 100 ng/mL bone morphogenetic protein-4 (BMP4) (PeproTech, 315-27). The osteogenic differentiation medium was replaced every second day.
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bioRxiv - Cell Biology 2022Quote: ... i3Neurons were cryo-preserved in i3Neuron media (BrainPhys Neuronal Medium supplemented [StemCell] with 2% B27 [Gibco], 10ng/mL BDNF [PreproTech], 10ng/mL NT-3 [PeproTech], and 1µg/mL Laminin [Corning]) with 10% DMSO added ...
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bioRxiv - Developmental Biology 2024Quote: ... the medium was changed to late hCNCCs medium by supplementing the early hCNCCs medium with 3 µM CHIR 99021 (Selleckchem, catalog no. S2924) and 50 pg/mL BMP2 (Peprotech, catalog no. AF-120-02) to enhance cell proliferation and reduce migration28 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were detached using TrypLE and seeded on nongelatinized bacterial dishes for suspension culture at 3 million cells per 75 cm2 and cultured in N2B27 containing 10 ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant human IL-7 and IL-15 (PeproTech) and 10 U/mL recombinant human IL-2 ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant murine IL-7 and IL-15 (PeproTech). One day prior to activation of OT-I cells ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated OPCs were then cultured on PDL-coated plates using GM plus 5 ng/ml FGF (450-33, Peprotech), 4 ng/ml PDGF-AA (315-17 ...
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bioRxiv - Cell Biology 2021Quote: Immunofluorescent staining was in general performed on HUVECS cultured on 12 mm glass coverslips coated with 5 μg/ml FN and treated with or without o/n TNFα (10ng/ml) (Peprotech), washed with PBS+/+(1mM CaCl2 and 0.5mM MgCl2) ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNFα (10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... for Th1 polarization the medium was supplemented with 5 ng/ml IL-2 and 10 ng/ml IL-12 (both from Peprotech), and 10 μg/ml anti-IL-4 (11B11 ...
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bioRxiv - Cancer Biology 2022Quote: ... mAb in 200ul of T cell media supplemented with 5 ng/ml of IL-7 and 30 U/ml of IL-2 (PeproTech) for 4 and 7 days ...
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bioRxiv - Immunology 2020Quote: ... adherent monocytes were differentiated into macrophages over a 5 days in the presence of 50 ng/mL GM-CSF (Peprotech) in RPMI supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
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bioRxiv - Genetics 2020Quote: ... and plated on Matrigel-coated plate in a density of 5×104 cells cm-2 in ES cell medium containing 10 ng/mL fibroblast growth factor 2 (FGF2, Peprotech). The differentiation was started by replacing ES cell medium with KSR medium and gradually switched to N2 medium from day 4 to day 10 ...
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bioRxiv - Immunology 2020Quote: Adherent PBMC monolayer was washed twice with HBSS and monocytes were differentiated into hMDMs for 5 days in RPMI 1640 containing 100 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech) and 10% foetal calf serum ...
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bioRxiv - Developmental Biology 2020Quote: ... treated with 5 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 1 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were incubated with StemPro-34 medium from days 2 to 5 with a cocktail of growth factors: vascular endothelial growth factor (VEGF; PeproTech), BMP-4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...