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Citations for Peprotech :
1501 - 1550 of 1701 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... splenocytes were adjusted to a concentration of 3 x 106 cells/mL in T cell medium containing 10 ng/mL rhIL-7 (Peprotech) and 2 µg/mL anti-mouse CD28 (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... Every 48 hours the cells were centrifuged and the pellet was resuspended between 1 and 2 × 106 per mL in fresh complete media containing the OVA peptide 3 μg/mL and IL-2 (100 U/mL) after the second stimulation IL-15 (10 ng/mL, Peprotech) was added The number of stimulations is indicated in the figure/text ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 ng/ml FLT3-L, 20 ng/ml IL-3 and 20 ng/ml G-CSF; all purchased from Peprotech) supplemented with 1% penicillin–streptomycin (ThermoFisher Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... for 6 hours in complete RPMI medium supplemented with 10 ng/mL recombinant IL-5 (PeproTech). T cells were sorted by flow-cytometry and labelled with CellTrace CFSE Cell Proliferation Kit (C34554 ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Cells were rested for 5 days in complete media with 10 ng/ml IL-7 (Peprotech) and then restimulated with TransAct ...
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bioRxiv - Immunology 2021Quote: ... and were stimulated with mIL-2 (200 U/mL) and hTGF-β1 (5 ng/mL, PeproTech) for iTreg differentiation ...
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bioRxiv - Neuroscience 2024Quote: ... we added a 5 µL droplet of 20 µg/ml of human laminin (RL511-175, Peprotech) to the center of each well and incubated for 2 hours at 37℃ ...
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bioRxiv - Cancer Biology 2022Quote: ... OCI-AML-5 culture media was supplemented with 10 ng/mL of recombinant GM-CSF (Peprotech) or 5637 conditioned medium ...
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bioRxiv - Immunology 2024Quote: ... Resting cells were supplemented with 5 ng/ml recombinant murine IL-7 (Peprotech 217-17-10). In some experiments ...
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bioRxiv - Cell Biology 2024Quote: ... 5 mg/mL insulin (Lantus, Sanofi-Aventis, Germany) and 10 ng/mL TGF-β1 (Peprotech, Germany).
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was washed with PBS and plated in DMEM with 10 ng mL-1 M-CSF (Peprotech Inc, 315-02). Following 5 days of culture ...
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bioRxiv - Bioengineering 2021Quote: ... HepatoSTIM™ medium was supplemented with 10 ng mL−1 of recombinant human TGF-β1 (100-21; PeproTech®, Rocky Hill, NJ), 100 ng mL−1 of recombinant human GDF-15 (120-28C ...
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bioRxiv - Immunology 2022Quote: ... in 500 μL of D10 medium in 24 well plates were stimulated for 7 days at 37°C with anti-mouse CD3 (1 µg/mL) and CD28 (0.5 mg/mL) antibodies plus mouse IL-6 (20 ng/mL) (Peprotech, Rocky Hill, NJ), and human TGF-β1 (2 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... 235 μL of complete DMEM supplemented with 1 % rat serum with or without chemotactic stimuli 30 ng/mL CXCL10 (PeproTech, 400-33) and CCL5 (PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... 100ng/mL cluster of differentiation 200 (CD200; Novoprotein # C31150UG) and100ng/mL fracktaline chemokine C-X3-C motif ligand 1 (CX3CL1; Peprotech # 300-31) (MBM + 5 GFs) ...
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bioRxiv - Systems Biology 2020Quote: ... on Day 1 we changed the medium to RPMI + 2% B-27 (minus insulin) + 5ng/ml BMP4 (Peprotech, cat. AF-120-05ET) + 1uM Chiron 99021 and incubated these cells for 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and incubated in naïve dedifferentiation medium of mTeSR-1 supplemented with 12 ng/mL bFGF (Peprotech #AF-100-18B), 1 µM PD0325901 (MEKi ...
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bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...
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bioRxiv - Neuroscience 2023Quote: ... L-glutamine and 1% Penicillin-Streptomycin (v/v) supplemented with CHIR-99021 (Stemgent, 0.5 μM) and brain derived neurotrophic factor (BDNF, PeproTech, 20 ng/ml). On the day in vitro 50 ...
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bioRxiv - Bioengineering 2023Quote: ... OPCs were cultured using OPC maintenance/proliferation media (OMM) composed of N2B27 supplemented with 10ng/ml insulin-like growth factor (IGF-1; Peprotech, 100-11), 15ng/ml platelet-derived growth factor AA (PDGFaa ...
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bioRxiv - Genomics 2024Quote: ... and the following day cells were fed with neural crest long-term medium (neural crest maintenance medium + 50 pg ml−1 BMP2 (PeproTech, 120-02) + 3 μM CHIR-99021 (Selleck Chemicals ...
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bioRxiv - Neuroscience 2024Quote: ... cells were resuspended in 320 μl of fresh DRG medium (F12, 10% hi-FBS, 1% Pen/Strep, 20 ng/ml mouse GDNF (Peprotech #450-44), 50 ng/ml mouse NGF (Peprotech #450-34) ...
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bioRxiv - Neuroscience 2024Quote: ... and the bottom chamber was prepared by adding migration medium with or without mouse VEGF-A (PeproTech Inc., 10 ng ml−1). The inserts were then put in an empty 24-well plate (BD Falcon ...
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bioRxiv - Cancer Biology 2024Quote: ... were cultured in 6-or 12-well plates for 24-72 hours with or without 1-100 ng/ml IFNγ (PeproTech, Cranbury, NJ), 10 µM benzo(a)pyrene (B(a)P)(Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... the latter HENSM conditions were supplemented with 4-20 ng/ml recombinant human ACTIVIN A (Peprotech 120-14E). We avoided using β-mercaptoethanol or adding additional BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40 ...
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bioRxiv - Bioengineering 2022Quote: ... M0 macrophages were polarized with 20-nM PMA and 10-ng/mL IL-4 (Peprotech, Rocky Hill, NJ) for two days to induce an anti-inflammatory phenotype ...
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bioRxiv - Immunology 2023Quote: ... B cells were cultured in U-bottom 96-well plates at a concentration of 1×106/ml with anti-IgM (1μg/ml, Jackson Immuno), anti-CD40 (1μg/ml, Miltenyi) and IL-4 (40ng/ml, Peprotech) stimulation for four days at 37°C in a humidified incubator with 5% CO2.
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bioRxiv - Cancer Biology 2023Quote: ... containing human embryonic stem cell (hESC) medium supplemented with 4 ng/ml bFGF (PeproTech, AF-100-18B-50) and 50 µM Rho-associated protein kinase (ROCK ...
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bioRxiv - Genomics 2024Quote: ... mesoderm cells (on day 4) were cultured in N2B27 medium supplemented with 10 ng/ml PDGF-BB (PeproTech) and 2 ng/ml Activin A (PeproTech ...
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bioRxiv - Microbiology 2021Quote: ... cells were starved for 3 h by removal of serum from the medium and then stimulated in duplicate with TNF-α (40 ng/ml, PeproTech) in FBS-free DMEM for 0 ...
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated onto 24-well plates in Matrigel and cultured for 3 days before treatment with either recombinant murine INF-y (PeproTech, UK) at 1 ng/mL or recombinant murine TGFβ 1 (R&D ...
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bioRxiv - Cell Biology 2023Quote: ... On day 3, medium was replaced with E6 supplemented with 50 ng/mL vascular endothelial growth factor (VEGF, (PeproTech, 100-20), 10 ng/mL FGF2 and 20 ng/mL rBMP4 ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol) with 100 ng/ml of human FMS-like tyrosine kinase 3 ligand (FLT3L, PeproTech, Cranbury, NJ, USA) for 7 days ...
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bioRxiv - Cell Biology 2021Quote: ... the transwells were dipped twice in PBS and transferred to a 6-well plate containing NSC medium (DMEM:F12 – glutamax, 1% pen/strep, 10 ng/mL EGF (Peprotech, AF-100-15-A), 10 ng/mL FGF (Peptrotech ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were incubated in RPMI1640 for THP-1 culture supplemented with human M-CSF at 20 ng/ml (cross-react with mouse, Peprotech, Cranbury, NJ, USA). After 2 days of culture ...
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bioRxiv - Physiology 2022Quote: ... to trigger their differentiation towards a pro-inflammatory phenotype M(LPS,IFN) or IL4 (20 ng ml−1, rat recombinant; Peprotech, London, United Kingdom) to obtain anti-inflammatory macrophages ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Microbiology 2023Quote: ... for 48h at a concentration of 1 bead/cell in the presence of 100 U/ml IL-2 (PeproTech, Inc., Cat. #200-02) in RPMI +/+ and were subsequently cultured under hypoxia (HOX - 1% O2 ...
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bioRxiv - Immunology 2024Quote: Purified monocytes were plated at a concentration of 1 million cells/mL in 96 U-bottom wells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin with 10 μg/mL of GM-CSF (Peprotech, cat #300-03). Cells were then cultured for 16-20h with or without stimulation.
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Immunology 2024Quote: ... cells were cultured in RPMI 1640 supplemented with 10% FBS and 1X Pen/Strep in the presence of either 1 ng/mL Recombinant murine IL-7 (PeproTech, Cat#217-17) or stimulated with anti-CD3/CD28 Dynabeads at a 4:1 bead:cell ratio (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 1×106 cells were plated in R10 containing 10 ng/mL recombinant murine M-CSF (Peprotech; Cat# 315-02) for 2 days ...
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bioRxiv - Immunology 2024Quote: ... Additional cytokines or anti-cytokine antibodies were used for the specific skewing into Th1 or Th2 cells: IL-12p70 (1 ng/mL; Peprotech, catalog# 210-12), IFN-γ (1 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... FACS-isolated Flk1+ mesodermal cells were cultured at 5 x104 cells/cm2 in SP34 plus SCF (Peprotech) and VEGF (R&D) ...
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bioRxiv - Immunology 2022Quote: ... and cells were plated at 5 × 105 cells/10 ml with 20 ng/ml M-CSF (PeproTech). On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... After 6 hours of serum starvation cells were stimulated with recombinant mouse LIF (5 ng/mL, Peprotech). Cells were lysed with Pierce RIPA buffer (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated FCS (LabTech #80837) and 50 ng/ml M-CSF (PeproTech #300-25) for 7 days.