Labshake search
Citations for Peprotech :
1551 - 1600 of 1701 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The wells of a 48-well-plate were coated with 5 μg/ml vitronectin (Peprotech, 140-09) diluted in PBS to increase cell attachment to the surface of well plate in the serum substitute medium [19] ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated FCS (LabTech #80837) and 50 ng/ml M-CSF (PeproTech #300-25) for 7 days.
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bioRxiv - Immunology 2024Quote: Cells from spleens and small intestine lamina propria were stimulated with 5 ng/ml IL-12 (Peprotech) plus 25 ng/ml IL-18 (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... 69 Co-cultures were performed in the presence of 5 ng/mL Flt-3L (Peprotech, 250-31L) and 1 ng/mL IL-7 (Peprotech 217-17 ...
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bioRxiv - Immunology 2021Quote: ... A similar procedure was done by replacing IFN-γ with IL-4 (10 ng/ml, Peprotech, Cat#214-14). To induce ASCs ...
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bioRxiv - Immunology 2023Quote: ... and tumors were induced one week later by topical application of 4-hydroxy-tamoxifen (0.5ug/graft in DMSO)(Peprotech) at the graft site ...
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bioRxiv - Neuroscience 2023Quote: ... Medium changes were done every 48 hours with astroglia medium containing 10 ng/mL BMP-4 (PeproTech, 120-05ET), 10% N2 supplement (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... and cultured for at least 48 hours (as indicated) with N2B27 + 20 ng/µL BMP-4 (Peprotech 315-27). For CUT&Tag adherent ASCs were detached by rinsing once with 1X PBS (without Ca2+/Mg2+ ...
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bioRxiv - Immunology 2024Quote: ... followed by culturing in exosome-free media (EFM) supplemented with 20 ng/mL of recombinant human IL-4 (Peprotech) to produce IL-4 conditioned media (19).
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bioRxiv - Cell Biology 2020Quote: ... from serial transplants were thawed and maintained in medium (DMEM with 15% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13), 10ng/ml IL-6 (Peprotech 216-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml murine Interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated in 5% CO2 at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were seeded in 24-well-plates at a density of 3×105 cells per well and treated either with a serial dilution series of IFNγ (PeproTech, 300-02) (starting at 50 ng/ml in two-fold dilution steps ...
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bioRxiv - Cell Biology 2020Quote: ... were maintained in medium (RPMI with 20% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13). For in vivo experiments ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved for 3 h prior to ligand stimulation with EGF (50 ng/mL, 10 min; PeproTech, Rocky Hill, NJ, USA), and were lysed in ice-cold lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... EBs cell culture media was replaced with X-VIVO 15 media supplemented with 50 ng/ml Flt-3-ligand (PeproTech, 300-19), 50 ng/ml M-CSF (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... bone marrow precursor cells were obtained from flushed femurs and tibias of C57BL/6J mice and cultured at 3*106 cells/ 10 ml in petri dishes in fully supplemented RPMI and rmGM-CSF (200 U/ml; Peprotech, Hamburg, Germany). Medium was exchanged on days 3 and 6 and the cells were cryopreserved on day 7 for further use.56
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were differentiated into primary human osteoclasts over 10 days, with media refreshed every 2-3 days and receptor activated nuclear factor κB ligand (RANKL, 25 ng/µL (PeproTech, London, UK) added on differentiation day 8 to stimulate osteoclast formation.
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bioRxiv - Immunology 2020Quote: ... Cells were then plated into Petri dishes and cultured in BMDC differentiation medium (RPMI, 10% FBS, 1% penicillin-streptomycin, 1x sodium pyruvate, 1x β-mercaptethanol) supplemented with GM-CSF (Peprotech, Rocky Hill, NJ). Media was refreshed on day 2 ...
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bioRxiv - Cancer Biology 2021Quote: Primary mouse or NMR fibroblasts were seeded at 1 × 104 cells/well onto 24-well plates and stimulated with TNF-α (PeproTech; 50 ng/mL), z-VAD-fmk (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... 2.5×105 HUVECs were plated in wells of 6-well plates and stimulated overnight with 20 ng.ml−1 human recombinant TNFα (PeproTech/Tebu Bio, #300-01A-A) or infected at MOI=200 with Neisseria meningitidis for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 1% FBS- containing DMEM for 72 hrs with or without 4ng/mL human recombinant TGF-β (Peprotech; cat #100-21).
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bioRxiv - Immunology 2021Quote: ... in antibiotic-free RPMI-1640 medium supplemented with 10% FBS and murine IL-5 (10 ng/mL, PeproTech). 108 bioluminescent C ...
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bioRxiv - Neuroscience 2020Quote: ... OPCs were then cultured on PDL-coated plates with GM plus 5 ng/ml FGF (#450-33, Peprotech), 4 ng/ml PDGF-AA (#315-17 ...
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bioRxiv - Immunology 2021Quote: ... the PSCs were resuspended at 100,000 cells/mL in the BDM supplemented with 5 ng/mL BMP4 (Peprotech) and plated at 20 uL/drop for inverted culture in 15 cm dishes ...
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bioRxiv - Immunology 2023Quote: ... were incubated for 24 hours in RPMI-10 supplemented with IL-7 (5 ng/mL, Peprotech 217-17). T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S ...
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bioRxiv - Immunology 2022Quote: ... and cultured at 37 °C in 5% CO2 with the indicated amounts of recombinant mouse IL-15 (PeproTech).
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bioRxiv - Immunology 2023Quote: ... The NK cells were counted and were resuspended in cRPMI supplemented with 5 ng/ml rhIL-15 (PeproTech) and rested overnight in a 96-well U-bottom plate at 37 °C and 5 % CO2.
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bioRxiv - Bioengineering 2024Quote: ... After day 5 the media was changed to RPMI containing interferon gamma (IFN-γ) (PEPROTECH, 300-02-20UG), and 100 ng/ml Lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2024Quote: ... splenic and lymph node single cell suspensions were cultured with Interleukin 7 (IL-7) (5 ng/ml, PeproTech) in complete RPMI 1640 medium ...
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bioRxiv - Immunology 2024Quote: ... A portion of the mixed naïve T cells maintained in recombinant mouse IL-7 (5 ng/mL, Peprotech) to verify baseline T cell ratios over time in culture.
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bioRxiv - Immunology 2024Quote: ... 200 IU/ml Penicillin-Streptomycin and 5 ng/ml granulocyte macrophage colony-stimulating factor (GM-CSF) (Peprotech, UK) for 7 days at 37°C.
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bioRxiv - Cell Biology 2024Quote: ... 5 mg/mL insulin (Lantus, Sanofi-Aventis, Frankfurt, Germany) and 10 ng/mL TGF-β1 (Peprotech, Darmstadt, Germany)) ...
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bioRxiv - Immunology 2021Quote: ... BM cells isolated from BALB/c mice were cultured for 6 days in medium supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (Peprotech) to generate DCs ...
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bioRxiv - Cell Biology 2021Quote: ... 100 U/ml penicillin and 100 µg/ml streptomycin sulfate (Nacalai Tesque) and 4 ng/ml basic fibroblast growth factor (Peprotech).
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bioRxiv - Neuroscience 2022Quote: ... in 100 μl embryoid body medium (10 mM ROCK inhibitor, 50 ng/mL BMP-4 (Peprotech; Cat. No. 120-05), 20 ng/mL SCF (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: Human liver organoid cultures derived from healthy and HBV infected livers were seeded and cultured for 4 days in EM without Wnt3a-conditioned medium supplemented with 25ng/ml of BMP7 (Peprotech). Hepatic differentiation was induced by culturing human liver organoids in differentiation medium (DM ...
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bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Immunology 2022Quote: Single bone marrow cell suspensions were plated at 4×106 per well in 2 mL of complete IMDM media supplemented with 150 ng/mL of FLT3L (PeproTech). On day 7 of differentiation ...
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bioRxiv - Immunology 2024Quote: ... R&D), IL-27 (20 ng/mL, R&D) as well as with 2 μg/mL anti-mouse IL-4 (Peprotech). Alternatively ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Neuroscience 2024Quote: ... and IFN-γ (20 ng/mL) (M1 stimulation) or IL-4 (20 ng/mL) and IL-13 (15 ng/mL, all from Peprotech) (M2 stimulation).
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bioRxiv - Molecular Biology 2024Quote: ... followed by an additional 4 hours with or without an apoptotic stimulus of 10 ng/ml TNFα (Peprotech, Cranbury, NJ) and 20 μg/mL CHX ...
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bioRxiv - Immunology 2024Quote: ... 100 units/mL penicillin and 100 μg/mL streptomycin] containing IL-4 (20 ng/mL) and GM-CSF (20 ng/ml) (PeproTech) and cultured for 5d with replacement of half of the medium and addition of fresh cytokines every other day ...
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...