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Citations for Cell Signaling Technology :
3651 - 3700 of 4408 citations for Mouse Vang Like Protein 2 VANGL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... HRP-linked goat anti-rabbit IgG (H+L) antibody (7074) and HRP-linked goat anti-mouse IgG (H+L) antibody (7076) were purchased from Cell Signaling Technology. Anti-mCherry antibody (SAB2702291) ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies [HRP-conjugated anti-mouse IgG (1:5000; Cell Signaling Technology; #7076) and HRP-conjugated anti-rabbit IgG (1:5000 ...
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bioRxiv - Bioengineering 2024Quote: ... 45 µL of cells suspension were incubated for 90 min at RT in a total volume of 55 µL with 10 nM BioGCSFR and 1:200 Myc-Tag (9B11) Mouse mAb (2276, Cell Signaling Technology). After one wash with 500 µL PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were rinsed three times for 10 minutes and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse antibodies (1:2,500, Cell Signaling, #7074, #7076) at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels were stained with primary antibodies (mouse anti-KRT7, 1:300, NSJ Bioreagents catalog #V2656; rabbit anti-Ki67, 1:400, Cell Signaling catalog #9129 ...
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bioRxiv - Physiology 2024Quote: ... which was blocked using Odyssey blocking buffer (Li-Cor, USA). Membranes were incubated with primary antibodies (GAPDH, Cell Signaling, Cat. 97166 [D4C6R], mouse mAb, 1:1000; (A-Tubulin, Cell Signaling, Cat ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with Blocking One (Nacalai Tesque, Inc.) for 90 min and then reacted overnight with mouse anti-Myc monoclonal antibody (9B11, Cell Signaling Technology) (1:1000) ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... cells were incubated in the blocking solution with the HRP linked anti-mouse or anti-rabbit secondary antibody (respectively #7076 or #7074; Cell Signaling Technology) at 1:10,000 dilution (1 hr at RT in the dark) ...
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bioRxiv - Bioengineering 2024Quote: ... membranes were washed three times with TBS-T and incubated with HRP-conjugated goat anti-mouse (1:1,000, Cell Signaling Technology #7056) or goat anti-rabbit (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated for 45 min with 3 μg/ml Myc-Tag (9B11) Mouse mAb AlexaFluor 488 conjugate antibody (Cell Signaling Technology) and Phalloidin-Alexa 647 (Invitrogen ...
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bioRxiv - Pathology 2022Quote: Cardiac proteins were extracted using freeze crusher (SK-100, Tokken Inc., Chiba, Japan) and cell lysis buffer (#9803, Cell Signaling Technology, MA, USA). Protein lysate was separated by SDS-PAGE and transferred onto polyvinylidene fluoride membranes ...
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bioRxiv - Biophysics 2021Quote: The phosphorylation of proteins was quantified by western blotting using antibodies against pEGFR (#4407 for pY1173 and #3777 for pY1068; Cell Signaling Technology (CST)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Aliquots of the cell lysates containing 50 μg of protein were submitted to SDS-PAGE and immunoblotted for TP53 (Cell Signaling Technology, #32532) and β-actin (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed on 6% acrylamide SDS-PAGE gels followed by immunoblotting with rabbit anti-myc (1:3000, 71D10, Cell Signaling Technology, 2278S). In vitro dephosphorylation experiments were performed in three biological replicates.
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bioRxiv - Neuroscience 2019Quote: ... Formalin fixed brains were paraffin embedded and analyzed using the following antibodies according to immunohistochemistry protocols described previously (Chakrabarty, 2010; Chakrabarty, 2015): Glial Fibrillary Acidic Protein (GFAP, Cell Signaling, 1:1000) and ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cancer Biology 2019Quote: ... 15 μg of extracted proteins were separated by SDS-PAGE and revealed by antibodies directed against actin (1: 1000, Cell Signaling Technology #8457), GAPDH (1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein of interest was detected using an appropriate antibody specific for phospho-EGFR (Tyr1068) (2234; Cell Signaling Technology [CST], Danvers, MA, USA), EGFR (2232 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Microbiology 2021Quote: ... was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA) substrates (Cell Signaling Technology, Danvers, MA) was used for western blotting at a dilution of 1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... phosphorylated at Ser293 PDHA and acetylated proteins were estimated by western-blotting using primary antibodies from Cell Signaling Technology (Danvers, MA, United States) with the product numbers #5490 ...
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bioRxiv - Immunology 2022Quote: ... Cas9 expression was validated by flow cytometry using intracellular staining of cas9 protein with anti-cas9 monoclonal antibody (7A9-3A3, Cell Signaling, cat #48796). Cas9 activity was assessed by transducing the cas9-BV2 cells with pXPR_047 ...
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bioRxiv - Genomics 2023Quote: ... Correct expression of the mAID-Dam-Lamin B1 fusion protein as well as the wildtype Lamin B1 protein was confirmed using Immunofluorescence staining using an anti-HA antibody at 1 in 500 dilution (Cell Signaling Technologies, C29F4) and an anti-LaminB1 antibody at 1 in 500 dilution (Abcam ...
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bioRxiv - Genetics 2022Quote: ... We quantified changes in protein levels relative to control using Image Lab software after normalization to β-actin or GAPDH (Cell Signaling Technologies), as indicated ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used at the indicated concentrations: Ubq (1:5000, P4D1, Cell Signaling; 1:5000, P4G7-HRP, BioLegend), Actin (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Biophysics 2023Quote: ... mAb #3288) and Lysosome Associated Membrane Protein 1 (LAMP1) specific rabbit monoclonal antibody (cat. no. mAb #9091) were purchased from Cell Signaling Technology (USA). Anti-caveolin-1 rabbit polyclonal antibody was acquired from BD Biosciences (USA ...
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bioRxiv - Biochemistry 2024Quote: ... Bound p52 and His-Bcl3-4E proteins were visualized by immunoblotting using anti-NF-κB p100/p52 antibody (Cell Signaling Technology, Cat#4882) and anti-His antibody (Bio Bharati Life Science ...
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bioRxiv - Cancer Biology 2024Quote: ... and Western blot performed on resultant protein as described by us.[85] Samples were probed using antibody against cleaved caspase 3 (Cell Signaling Technology, #9664) and β-actin (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for 20 hours with a protein complex of anti-HA antibody (Cell signaling technology, C29F4) and Sera-Mag SpeedBeads Protein A/G (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used to detect respective proteins by incubation overnight at 4°C in 5% BSA: CD44 (#E7k2y, Cell signaling, 1:1000), RHAMM (#E7S4Y ...
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bioRxiv - Biochemistry 2020Quote: ... #2983), Histone H3 (1:10,000, #4499), MEK1/2 (1:1000, #8727) and LDH (1:5000, #2012;) were from Cell Signaling Technology, Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... and incubated overnight at 4°C with the primary antibodies Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370S, Cell Signaling) at 1:2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Other commercially available primary antibodies used were: anti-Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit antibodies for anti-myosin-light-chain-2 (Cat #3672) and anti-phospho-myosin-light-chain-2 (raised against phospho-Thr18/Ser19; Cat #3674) were purchased from Cell Signaling and used at 1:500 (Western blot) ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining sample was incubated o/n on a rotary shaker at 4 °C with 2 μg of the appropriate antibody (IgG, 12-370 Merck; pSTAT1, 7649 Cell Signaling). The next day 25 μl Dynabeads resuspended in CDB/c were added and incubated for at least 1 h at 4 °C on a rotary shaker ...
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bioRxiv - Cell Biology 2022Quote: ... then seeded in DMEM/F12 and 1% serum plus antibiotic-antimycotic solution 48 hrs post-transfection cells were blocked and treated with Ab against α5β1 (Novus, 2-52680) and αv (Cell Signaling 4711) at 10ug/ml for 30 minutes prior to cell surface stripping (0.2M acetic acid ...
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bioRxiv - Immunology 2021Quote: ... followed by washing and 2 hour incubation with goat-anti-rabbit-HRP antibody at 1:5000 (all from Cell Signaling Technology). Membranes were washed again and detected with SuperSignal West Pico Chemiluminescent substrate (Thermofisher) ...
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bioRxiv - Biophysics 2021Quote: ... and phospho-myosin (rabbit phospho-myosin light chain 2 (Thr18/Ser19) antibody (dilution 1:50 in PBS 0, 5% BSA, #3674, Cell Signaling) for 2 h at RT ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Plant Biology 2021Quote: ... the membrane was incubated overnight with the primary phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, #9101). Subsequently ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked and incubated with an antibody recognizing Myosin Light Chain 2 phosphorylated at serine19 (1:200, rabbit; Cell signaling technology, #3671), or with the anti-tyrosinated tubulin antibody YL1/2 (1:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... Pre-cleared lysates were incubated over night at 4°C with end-over-end rotation with 2 µg of anti-Notch1 (D1E11, Cell Signaling) or anti-Cbl-b (G1 ...
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bioRxiv - Genetics 2022Quote: ... Beads were then resuspended in 250 μl blocking solution and 2 μg antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were probed overnight at 4 °C with antibodies against the following epitopes: phospho(Ser217/221)-MEK1/2 (1:1000; 41G9; Cell Signaling), MEK1/2 (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Where stated, cells were treated with 2 μM ruxolitinib (INCB018424, Stratech, S1378-SEL) or 50 nM bortezomib (Cell Signaling Technology #2204) or 1000 units of IFNβ (pbl Assay Science ...
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bioRxiv - Genetics 2020Quote: ... and bound Myc bead (1/2 total amount) samples were analyzed by SDS-PAGE and immunoblotting with rabbit anti-Myc monoclonal antibody (1:2000; Cell Signaling) to detect Myc-tagged EXOSC5 and EXOSC2 ...
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bioRxiv - Bioengineering 2020Quote: ... 1 x 108 induced cells were stained with 1 µM of directly AF488-labeled SARS-CoV-2 RBD and 0.5 µM anti-HA AF647 (Cell Signaling Technology) antibody ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Pathology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694 ...