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Citations for Cell Signaling Technology :
3501 - 3550 of 4408 citations for Mouse Vang Like Protein 2 VANGL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 20 µg of total brain protein were loaded and anti-GFAP antibody (D1F4Q) XP Rabbit mAb (1:3000; Cell Signaling Technology, 12389), anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of PSMA protein in each cell line was investigated by western blot using anti-PSMA rabbit antibody (Cell Signaling Technology) [11].
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bioRxiv - Immunology 2020Quote: ... The cell lysates were incubated overnight at 4 °C with NEMO antibody and ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology).
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Biophysics 2022Quote: ... 15 μg of total protein was loaded onto an SDS-PAGE (4-12%) to assess LRP6 and β-catenin protein levels using phospho- and total LRP6 (Cell Signaling), active- and total β-catenin (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Microbiology 2024Quote: ... the ADP-ribosylated proteins were visualized using chemiluminescence using the SignalFire ECL Reagent or the SignalFire Elite ECL Reagent (Cell Signaling Technology) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: MCF10A cells from 2D collagen stimulation assays were pelleted by centrifugation and lysed using 1X RIPA Buffer containing both 1x protease inhibitor cocktail and 1x phosphatase inhibitor Lysate was precleared with Protein-A Magnetic Beads (73778, Cell Signaling Technology).
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bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then the supernatant fraction was incubated with anti-BCR-ABL antibody overnight at 4°C and subsequently with protein A magnetic beads (73778, Cell Signaling Technology) for another 4 h at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then prepared for Western blot analysis and total protein was incubated with E-Cadherin (1:1000, Cell Signaling Technology, #3195), Fibronectin (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated proteins were detected by probing the membranes with an anti-phospho-c-Met antibody (Tyr1234/1235, #3077, Cell Signaling Technology) or an anti-phospho-EGFR antibody (Tyr1068 ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Bioengineering 2023Quote: ... Western blot analysis was performed to assess total and phosphorylated p70S6K1 (70kDa ribosomal protein S6 kinase) (T389, Cell Signaling Technology; Boston, MA) as well as 4EBP1 (4E-binding protein 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... HER2 protein detection on EVs was performed by staining samples with HER2/ErbB2 (29D8) – PE-conjugated antibody or Isotype Control (Cell Signaling Technology) and CellMask plasma membrane stains (C10046 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Biophysics 2023Quote: ... blocked with 5% bovine serum albumin for 1 hour at room temperature and incubated overnight with a primary antibody against the principal ventricular GJ protein Cx43 phosphorylated at Ser368 (pCx43, 1:1000, #3511S, Cell Signaling Technologies), at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Every gel included two molecular-weight markers for separate regions of interest (High Range Color-Coded Prestained Protein Marker; Cell Signaling Technology; and Novex Prestained Protein Standard ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
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bioRxiv - Immunology 2023Quote: ... cycloheximide (CHX) final concentration of 8 ug/mL was used to arrest protein translation and Actinomycin D (Cell Signaling Technology, Cat. 15021) was used to arrest transcription by treating cells with 10 ug/mL Actinomycin D where indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... or EV fraction was loaded alongside a biotinylated ladder and/or pre-stained protein molecular weight markers (Catalog numbers 7727 and 59329, respectively, Cell Signaling Technology) on NuPAGE™ Bis-Tris™ pre-cast gels (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... the resulting supernatants were incubated for 3 hours at 4°C with a 2.5 µL slurry of Sera-Mag SpeedBeads Protein A/G (Cytiva) pre-incubated with 2.5 µL of anti-HA-tag rabbit mAb (Cell signaling technology, C29F4). The beads were washed four times with the lysis buffer and then twice with 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2024Quote: ... Bis(sulfosuccinimidyl)suberate disodium salt (BS3; Dojindo, #B574) was used to crosslink the antibodies to protein G magnetic beads (Cell Signaling Technology). Benzonase (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... which were blocked by 5% non-fat milk (americanBIO, AB10109-01000) and then incubated overnight at 4°C with the following primary antibodies: Nucleocapsid protein (Cell Signaling, #33717), Strep-tagged Nucleocapsid protein (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT# ...
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bioRxiv - Immunology 2024Quote: ... Protein expression levels were detected with the following antibodies: Jak-1 (6G4) and phospho-Jak-1 (Tyr1034/1035) (from Cell Signaling Technology), IL-10Rα (A3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained at 1:100 with anti-phospho S6 ribosomal protein (Ser235/236) Alexa Fluor 647 (D57.2.2E, Cell Signaling Technologies cat#4851S).
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for more than 3 hours with a protein complex of anti-HA antibody (Cell signaling technology) and Dynabeads Protein A (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with 90% (v/v) methanol and then intracellularly stained with anti-phospho-p44/42 MAPK (Erk1/2; Cell Signaling) for 30 min at room temperature and then with secondary antibodies against rabbit IgG (H+L ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: The kinase inhibitors used were against cSrc (Dasatinib 100nM), AKT (Perifosine 10μM), ERK1/2 (U0126, 10μM) (0952, 14240 and 9903, respectively, Cell Signaling Technologies) and pNaKtide (1μM ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4°C with the rabbit anti-phospho-p44/42 MAPK (for pErk1/2) (Cell Signaling Technology) at 1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... Other primary antibodies used are anti–Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, 197G2, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated overnight with rabbit polyclonal primary antibodies against phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, 1:2000) at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were used: rabbit anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370, 1:100, Cell Signaling), rabbit anti-Phospho-Drosophila p70 S6 Kinase (Thr398 ...
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bioRxiv - Physiology 2022Quote: ... anti-p44/42 MAPK (Erk1/2) (#4695) and anti-Acetyl-α-Tubulin (Lys40) (#5335) antibodies were from Cell Signaling Technology. Anti-Lamin A/C (sc-20681) ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibodies used for immunofluorescence staining were as follows: anti-Phospho Myosin Light Chain 2 (Cell Signaling Technology, #3671), anti-ZO-1 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... and the plate was extensively washed with TBST + 100 mM EDTA and a 1:8,000 dilution of primary antibody (p-MEK1/2 (S217/S221) Rabbit Antibody (Cell Signaling)) was added to the plate and incubated for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... phospho-Erk1/2 T202/Y404 (4370) 1:2000 and Erk1/1 (4695) 1:2000-1:5000 (all from Cell Signaling); and Tubulin (T5168 ...
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bioRxiv - Cancer Biology 2021Quote: ... Zebrafish embryos were stained with primary antibodies directed against Phospho-p44/42 MAPK (Erk1/2) Thr202/Tyr204 (4370S, Cell Signaling) at 1:200 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% milk in PBS-T (0.1% Tween 20) and incubated with rabbit anti-mitofusin 2 (mAb D2D10, Cell Signaling), rabbit anti-mitofusin 1 (mAb 13196S ...
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bioRxiv - Cancer Biology 2022Quote: ... their nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 ug/ml, 4083S, Cell Signaling Technology, MA, USA) and imaged by using an LSM 880 laser scanning confocal microscope (Carl Zeiss AG ...
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bioRxiv - Cell Biology 2022Quote: ... phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370) and pS473-Akt (#4060) antibodies were from Cell Signaling Technology (CST). Further antibodies used were against GST (sc-138 ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was anti-p-ERK (anti-pERK MAPK [Phospho-p44/p42 MAPK, ERK1/2, Thr202/Tyr204, D13.14.4E], Cell Signaling Technology) that specifically recognizes the [T-X-Y] MAPK activation motif ...
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bioRxiv - Neuroscience 2023Quote: ... and were then incubated with 25 μg/mL of DAPI (4’,6-diamidino-2-phenylindole dihydrochloride; Cell Signaling Technology, USA) for 5 min ...
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bioRxiv - Systems Biology 2023Quote: ... Constant volumes of sample were incubated overnight with 2 μg α-BRD4 antibody (Cell Signaling, Danvers, MA, USA, Cat. # 13440) or a nonspecific isotype control (LSBio ...
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bioRxiv - Systems Biology 2023Quote: ... The following primary antibodies were used: i) Phospho-Myosin Light Chain 2 (Ser19) (1:50, Rabbit, Cell Signaling Technology #3671S) ii ...
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bioRxiv - Cell Biology 2023Quote: ... a rabbit monoclonal antibody phospho-p44/42 MAPK (Erk1/2, Thr202/Tyr204; cat no. 4376, Cell Signaling Technology, MA, USA) to detect phospho-Slt2 ...
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bioRxiv - Immunology 2023Quote: ... cells were blocked in 2% BSA-PBS and incubated with NF-κB p65 (1:100, Cell Signaling Technology, Cat# 6956S) followed by Alexa Fluor 488 conjugated chicken anti-mouse IgG (1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining supernatants were subjected to immunoprecipitation by incubation with 2 μl of anti-mTOR antibody (#2983, Cell Signaling Technology) for 3 hours (4 °C ...