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Citations for Cell Signaling Technology :
3901 - 3950 of 4408 citations for Mouse Vang Like Protein 2 VANGL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Forty-five μg of protein were resolved in SDS-PAGE and blotted with anti-phospho-eNOS (Ser1177) antibodies (Cell Signaling, Danvers, MA, USA, 1:250), followed by incubation with goat anti-rabbit horseradish peroxidase secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were then incubated at 28°C and the samples were collected at the indicated intervals for determination of GST and GST-WL1 protein abundance by western blots using the anti-GST (#2625, Cell Signaling Technology, 1:1000 dilution) and alkaline phosphatase-conjugated anti-rabbit (# 31340 ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg of protein per lane were subjected to SDS-PAGE and probed with the following primary antibodies: YAP (Cell Signaling Technologies #8418, 1:500), YAP/TAZ (Cell Signaling #4912 ...
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bioRxiv - Immunology 2020Quote: ... Antibodies used to detect proteins involved in glycolysis (Hk1 clone C35C4, Glut1 clone D3J3A and HIF-1a D1S7W) were from Cell Signaling (all at 1:500) were used to intracellular staining ...
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bioRxiv - Systems Biology 2022Quote: ... Immunoblot analysis was used to assess the adequacy of nuclear purification by measuring Lamin A/C (rabbit, 1:1000, Cell signaling # 2032, a nuclear protein).
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysates were extracted at 3 hours post-treatment and analysed by western blot following overnight incubation with antibodies against the following primary proteins: Vinculin (E1E9V, rabbit, Cell Signaling Technology, 13901, 1:1000), Phosphorylated p44/42 MAPK (ERK1/2 ...
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bioRxiv - Physiology 2023Quote: ... we loaded 25 µg protein of each sample to SDS-PAGE gels and incubated the membranes with 1:1000 eIF2α antibody (Cell Signaling Technology #9722, Danvers, MA) or 1:1000 Phospho-eIF2α antibody (Cell Signaling Technology #9721 ...
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bioRxiv - Neuroscience 2023Quote: ... which is consistent with other validation western blots published by vendors for this protein as well as manuscripts listed above (Cell Signaling Technologies, Novus, Rockland, Proteintech). The anti-SARS-CoV-2 spike protein antibody (GTX632604 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies to the following proteins were used at the indicated concentrations: ubiquitin (1:1000, P4G7, BioLegend; 1:200 Cell Signaling; 1:200, Z0458, Dako), GABARAP (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... at room temperature for 1 h and then incubated for 16 h with primary antibodies against the ER tracker protein disulfide isomerase (PDI; rabbit mAb #3501, Cell Signaling Technologies, Danvers, MA, USA) and a loading control GAPDH (rabbit mAb #2118 ...
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bioRxiv - Cancer Biology 2021Quote: ChIPs were performed using cross-linked chromatin from Capan-2 cells and either anti-BCL6 antibodies (1;1000, Cell Signaling Technology Cat# 14895, RRID:AB_2798638), anti-STAT1 antibodies (1;1000 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Cell Biology 2019Quote: ... Caco-2 cells were incubated overnight at 4°C with primary antibodies (1:100 dilutions with blocking buffer) for GRP78 (Cell Signaling, MA, USA) or CHOP (Santa Cruz ...
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bioRxiv - Physiology 2019Quote: ... phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and a-tubulin antibody (mouse,1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... then stained with Hoechst 33342 (2 µg/mL) in OBB and antibodies against p-ERK (1:200, Cell Signaling Technologies, Cat. No. 4370S), then anti-rabbit IgG (1:2000 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with TBS containing 2% (m/v) bovine serum albumin (BSA) and then incubated with antibodies raised against the HA epitope (Cell Signaling Technology, USA) diluted 1:1000 in the same solution ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... using SimpleChIP Plus Enzymatic Chromatin Immunoprecipitation kit (agarose beads) (Cell Signaling Technology, 9004). The enriched DNA was quantified by qPCR analysis using the primers listed in Supplementary Table 3.
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: ... Primary antibodies used were: DNA damage antibody sampler kit (Cell Signaling Technology, #9947), PLK1 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2019Quote: ... the signals were detected with a chemiluminescence phototope-HRP kit (Cell Signaling, USA). As necessary ...
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bioRxiv - Molecular Biology 2019Quote: Senescence assays were performed using the Senescence b-Galactosidase Staining Kit (Cell Signaling) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ChIP assay was conducted by using the ChIP assay kit (Cell Signaling Technology) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... ChIP was performed with Simple ChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP was performed with the SimpleChip Plus Kit with magnetic beads (Cell Signaling) per manufacturer’s protocol with the following exceptions ...
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bioRxiv - Physiology 2020Quote: ... using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads; Cell Signaling). Chromatin lysates were prepared ...
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bioRxiv - Cancer Biology 2021Quote: ChIP assay was conducted by using the ChIP assay kit (Cell Signaling Technology) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... SignalStain® Boost IHC Detection Reagent and DAB substrate kit (Cell Signaling Technology) were used and then the sections were counterstained with hematoxylin ...
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bioRxiv - Physiology 2020Quote: ... using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads; Cell Signaling). Liver from pups and adult control and Alb-D2KO mice were obtained as described (22) ...
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bioRxiv - Cell Biology 2021Quote: DRIP was performed using the SimpleCHIP enzymatic chromatin IP kit (Cell Signaling Technologies) in accordance with manufacturer’s instructions with some modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Cell Fractionation Kit (9038) was purchased from Cell Signaling Technology (Danvers, MA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Detection was performed using DAB substrate kit (8059S, Cell Signaling Technology, Danvers, MA). Samples were counterstained using Mayer’s hematoxylin (MHS32 ...
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bioRxiv - Bioengineering 2022Quote: CUT&RUN was performed using the CUT&RUN Assay Kit (Cell Signaling #86652). Briefly ...
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bioRxiv - Physiology 2022Quote: ... Proliferation was measured using the BrdU Cell Proliferation Assay Kit (Cell Signaling Technology). Migration was measured using the ORIS Cell Migration Assay Kit (Platypus Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... ChIP assay was performed using the ChIP Assay Kit (Cell Signaling Technology, 9003) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... all antibodies were from the NF-kB Pathway Sampler kit (Cell Signaling, 9936S) and β-actin was from Abgent (1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibodies were from NF-kappa B Pathway Sampler kit (Cell Signaling, 9936S). Immunodetection was performed with Chemiluminescent HRP Antibody Detection Reagent (Denville Scientific INC ...
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bioRxiv - Cell Biology 2022Quote: ChIP was performed using the SimpleCHIP enzymatic chromatin IP kit (Cell Signaling Technologies) in accordance with manufacturer’s instructions with some modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection was performed using DAB substrate kit (8059S, Cell Signaling Technology, Danvers, MA). Samples were counterstained using Mayer’s hematoxylin (MHS32 ...